Biostar Class - Sequencing Instruments
Below are a list of links and resources mentioned in the BIOSTAR Sequencing Instruments class given on 06/10/20 and 06/11/20
Sequencing Technologies - Company Web Sites
CCR Sequencing Related Resources
- Office of Science and Technology Resources (OSTR)
- Supplemental Technology Award Review System (STARS)
- Collaborative Research Exchange (CREx)
CCR Sequencing Resources
- NCI Sequencing Facility (SF) - ATRF, Frederick
- NCI CCR Genomics Core (GC) - Bethesda, Bldg 37
- NCI CCR Single Cell Analysis Facility (SCAF) - Bethesda, Bldg 37
- NCI Genomics Technology Laboratory - ATRF, Frederick
- NIH Intramural Sequencing Center (NISC) - Rockville
Experimental Considerations
Remember: Replicates, batch effects, consult BEFORE sequencing
- CCBR Best Practices for Experimental Design
- Sequence depth and machine requirements estimates can be obtained from the Illumina Sequencing Coverage Calculator
- Cost estimates can be previewed at the NCI Sequencing Facility
Illumina Sequencing Technology
- Illumina Sequencing Technology Video
- Introduction to Illumina Sequencing
- Summary of Illumina DNA-seq applications and library preparation methods
- Summary of Illumina RNA-seq applications and library preparation methods
Data Transfer Utilities
- Globus Data Transfer Utilities
- Data Sharing and Transfer Using Globus - NIH Biowulf/Helix
- CBIIT DME Documentation