FastQCFastQC Report
Wed 10 Aug 2022
BORED_1_R1.fq

Summary

[OK]Basic Statistics

MeasureValue
FilenameBORED_1_R1.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences112193
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGT8940.7968411576479815No Hit
CCGCTTTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAA7830.697904503846051No Hit
CTTTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATAT7730.688991291791823No Hit
CGCTTTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAAT7690.6854260069701319No Hit
CGGCTGTCGAGTTGTACGGCCGTTCAGCCACGAGTCACGGGGTCTAACGC7570.6747301525050583No Hit
CTGAGACAGAGTCGCTATCGTTATGTCTCCTTCCCGCGGTCAAGGCGAAA6490.578467462319396No Hit
GCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGA6250.557075753389249No Hit
GTAAAACGCAAGCACCGGCTGTCGAGTTGTACGGCCGTTCAGCCACGAGT5560.49557459021507577No Hit
CTCAGACGCTGCCCTAACTGCGCAGTTAATAATTCTGGCAATTCGTCTCC4800.42783417860294315No Hit
GGAAGCTATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTG4720.42070360895956077No Hit
CCCATTTTTAGTTATAATGATGCCTTATGTGATAGATGCCTCTTTAAAAT4310.38415943953722603No Hit
CCGGGATCGGGCAAAGGGGCAACTCGAGCTAATCTCCCCAGCGGCTAGCA3770.33602809444439496No Hit
GTTATGAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAA3700.3297888460064353No Hit
GCCTCACTTAGTTACAGTTTTATGGATAATTGGGATATTCTTTGGTATAG3630.32354959756847573No Hit
GTCTCCTTCCCGCGGTCAAGGCGAAACCGCAGCAAACTTCCTCAGACGCT3550.31641902792509335No Hit
CGACGAACAACGAAGAGCGACGATGCCCGTTTCAGGTGGTCCTCAGCGTA3430.3057231734600198No Hit
CGGGGAACAGTCGCATGACGAGGCAGTTTCCAGAAACGTGTATCACATCT3240.28878807055698663No Hit
CCCGTTTCAGGTGGTCCTCAGCGTACGGCGGGACCTCTGAGAATTGGGAT3220.287005428146141No Hit
ATCACATCTAGGCATGGAATCTTATGCCAGCTAACGGAACAAGCTTTGTG3190.2843314645298726No Hit
GCCATTCGGACCTACCGTAAGCCTATATTTCGTTTTTCTGAGACCTATCC3180.28344014332444983No Hit
CGGACAACTGATGGACCCACGTTGCGAGTCCAGTAAATCAGGACGGGGAT3180.28344014332444983No Hit
CCGAGTTCAGTGCGACCGTACAGCTCTGGAACCCAAAGGTTCGTTTTTTT3110.27720089488649025No Hit
GGGATAATTGCGCGGTTTTGACTGCGGAAGGAAGCCAAGGCCCACCTAAA3060.27274428885937624No Hit
CTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGGGATGAGCATAAACCA3030.27007032524310787No Hit
CTTACAAGTCCGCTCCTCGGGGAGCTTGATTGATAATTCTGTATAAGGTG2990.26650504042141665No Hit
AGGAGATGTTGGAACTCTACCAATTGGAGCTTTCTTAGCTGTCTTAGCAG2990.26650504042141665No Hit
CTCCTTCCCGCGGTCAAGGCGAAACCGCAGCAAACTTCCTCAGACGCTGC2880.25670050716176585No Hit
GGACAACTGATGGACCCACGTTGCGAGTCCAGTAAATCAGGACGGGGATA2820.2513525799292291No Hit
CCTCACTTAGTTACAGTTTTATGGATAATTGGGATATTCTTTGGTATAGT2820.2513525799292291No Hit
GCCCTAACTGCGCAGTTAATAATTCTGGCAATTCGTCTCCACACTAGAAG2820.2513525799292291No Hit
GTGTACACCGCCCTTAGACGTCTTGGTATACGGACAACTGATGGACCCAC2790.2486786163129607No Hit
CTCACTTAGTTACAGTTTTATGGATAATTGGGATATTCTTTGGTATAGTT2730.2433306890804239No Hit
GGGGTTATGAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGG2700.24065672546415554No Hit
CCTGACCCCGGGGAACAGTCGCATGACGAGGCAGTTTCCAGAAACGTGTA2660.23709144064246432No Hit
GTCGAGACTGAAAAGCTATAACCCGCAGACCCGAGCGAAAGCGGCGGTCC2610.2326348346153503No Hit
GCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGGGATGAGCATAA2600.23174351340992752No Hit
CTGTGATCTACTAGCGCCAATCTATATTACTCACAATATCCTAAAAACGA2590.23085219220450476No Hit
CCAGAATTCAAGACGTTAACAGTTCTTGGCGCAAATAGCGCTGAATCGCT2510.22372162256112235No Hit
GGCAGTTTCCAGAAACGTGTATCACATCTAGGCATGGAATCTTATGCCAG2500.22283030135569953No Hit
CCGGGGAACAGTCGCATGACGAGGCAGTTTCCAGAAACGTGTATCACATC2500.22283030135569953No Hit
CTGGGGTTATGAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGAT2480.22104765894485398No Hit
CGCCAATCTATATTACTCACAATATCCTAAAAACGATCAGCCGGCAGGTT2460.21926501653400837No Hit
CTCGGCAATAGGATTGTAGCCCAGGATGAGTCCCTCAGCGTGACGCAGTA2450.21837369532858555No Hit
ATTTTTAGTTATAATGATGCCTTATGTGATAGATGCCTCTTTAAAATATC2430.21659105291774No Hit
GGATAATTGCGCGGTTTTGACTGCGGAAGGAAGCCAAGGCCCACCTAAAG2360.21035180447978039No Hit
CGCATGACGAGGCAGTTTCCAGAAACGTGTATCACATCTAGGCATGGAAT2350.2094604832743576No Hit
CTATATTTCGTTTTTCTGAGACCTATCCGAGTTCAGTGCGACCGTACAGC2350.2094604832743576No Hit
GTTTTTCTGAGACCTATCCGAGTTCAGTGCGACCGTACAGCTCTGGAACC2350.2094604832743576No Hit
CTTTGGTATAGTTGGGATTTTAATATCATTAATAGCATGATGGTGATTGT2330.20767784086351196No Hit
GTCGGGGGCACCACACCACGCTCGCTTGGCAGTAACGCGAGCAGGGGGCG2320.20678651965808917No Hit
CCCTGACCCCGGGGAACAGTCGCATGACGAGGCAGTTTCCAGAAACGTGT2260.20143859242555243No Hit
CTGCGATAGGCTAGTTCATAAACGAGGGGCGATGCCCGGGCTCAGAATCC2250.2005472712201296No Hit
GTCGAGTTGTACGGCCGTTCAGCCACGAGTCACGGGGTCTAACGCCTACC2240.19965595001470682No Hit
GTCGGTTTAGCTGAGATTGCACCGTTGTTATAATCGACCCGGGCTGCGAC2220.19787330760386118No Hit
GGGGATAATTGCGCGGTTTTGACTGCGGAAGGAAGCCAAGGCCCACCTAA2210.1969819863984384No Hit
GTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGGGA2200.19609066519301563No Hit
GGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGAGATGT2150.19163405916590162No Hit
CTTTACAGCAAATACCAGCGGCCCTGACCCCGGGGAACAGTCGCATGACG2130.189851416755056No Hit
CTCAGCGTGACGCAGTAAACTAGTAGCATCCCGATCTTTTCCCTATCTAA2100.18717745313878764No Hit
TGCCATTCGGACCTACCGTAAGCCTATATTTCGTTTTTCTGAGACCTATC2040.18182952590625084No Hit
CTTAGTTACAGTTTTATGGATAATTGGGATATTCTTTGGTATAGTTGGGA2030.18093820470082803No Hit
GCCCGTTTCAGGTGGTCCTCAGCGTACGGCGGGACCTCTGAGAATTGGGA2030.18093820470082803No Hit
CTCTTTACAGCAAATACCAGCGGCCCTGACCCCGGGGAACAGTCGCATGA1960.17469895626286847No Hit
TCCGCTTTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAA1920.17113367144117725No Hit
TTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTA1920.17113367144117725No Hit
CGGCAATAGGATTGTAGCCCAGGATGAGTCCCTCAGCGTGACGCAGTAAA1900.16935102903033167No Hit
CGGCGCTCTTCCTAGTTGGTACAGAACTGAGTGTCATGTGTCCAAAGTTA1890.16845970782490885No Hit
GTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGATGCCTTATGT1880.16756838661948606No Hit
CGGCAGGTTCATTTTAAACGCGGTGACTAGGATGCTCATTTGAATGTCCC1860.16578574420864045No Hit
GGGGAACAGTCGCATGACGAGGCAGTTTCCAGAAACGTGTATCACATCTA1860.16578574420864045No Hit
CTGACCCCGGGGAACAGTCGCATGACGAGGCAGTTTCCAGAAACGTGTAT1840.16400310179779487No Hit
GTCTGCGATAGGCTAGTTCATAAACGAGGGGCGATGCCCGGGCTCAGAAT1830.16311178059237205No Hit
GGATATTCTTTGGTATAGTTGGGATTTTAATATCATTAATAGCATGATGG1830.16311178059237205No Hit
ATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACCGC1830.16311178059237205No Hit
CCACATTCAAATTGACCTCCCTCAGGAAGCTAAGAAATACTATCTCGGCA1810.16132913818152647No Hit
CCCACCTAAAGTATGCTGCAAACATATGCAGATCCCCTGAGACAGAGTCG1790.1595464957706809No Hit
CTTGGTATACGGACAACTGATGGACCCACGTTGCGAGTCCAGTAAATCAG1780.15865517456525807No Hit
GTTTTTTTCTTGGTACCTATTCCTCCAGGAATTACTGACCATAGTGCTCG1770.15776385335983528No Hit
CGCATATAACCACATTCAAATTGACCTCCCTCAGGAAGCTAAGAAATACT1760.1568725321544125No Hit
CGCAAGTTGTAGGTCGGGGGCACCACACCACGCTCGCTTGGCAGTAACGC1760.1568725321544125No Hit
CTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTT1750.15598121094898967No Hit
CGAGGATGTTCCTGTTGATACCGTCTGCGATAGGCTAGTTCATAAACGAG1750.15598121094898967No Hit
GGGATATTCTTTGGTATAGTTGGGATTTTAATATCATTAATAGCATGATG1750.15598121094898967No Hit
ATTCGGACCTACCGTAAGCCTATATTTCGTTTTTCTGAGACCTATCCGAG1740.15508988974356688No Hit
GTTGATACCGTCTGCGATAGGCTAGTTCATAAACGAGGGGCGATGCCCGG1740.15508988974356688No Hit
GTGCTAGCCCGTCGAGACTGAAAAGCTATAACCCGCAGACCCGAGCGAAA1730.1541985685381441No Hit
GGCAGGTTCATTTTAAACGCGGTGACTAGGATGCTCATTTGAATGTCCCC1700.1515246049218757No Hit
CCTCTTTACAGCAAATACCAGCGGCCCTGACCCCGGGGAACAGTCGCATG1690.1506332837164529No Hit
GGCAATTCGTCTCCACACTAGAAGTCGACGAACAACGAAGAGCGACGATG1660.1479593201001845No Hit
TGGCAATTCGTCTCCACACTAGAAGTCGACGAACAACGAAGAGCGACGAT1660.1479593201001845No Hit
CCCCTCTTTACAGCAAATACCAGCGGCCCTGACCCCGGGGAACAGTCGCA1660.1479593201001845No Hit
GTTGGAACTCTACCAATTGGAGCTTTCTTAGCTGTCTTAGCAGTAGTTTA1630.1452853564839161No Hit
CTACCGTAAGCCTATATTTCGTTTTTCTGAGACCTATCCGAGTTCAGTGC1630.1452853564839161No Hit
ATCTAATTCACCTCCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAA1590.14172007166222492No Hit
CCGCAGACCCGAGCGAAAGCGGCGGTCCTTACAAGTCCGCTCCTCGGGGA1590.14172007166222492No Hit
CCTGTTGATACCGTCTGCGATAGGCTAGTTCATAAACGAGGGGCGATGCC1580.1408287504568021No Hit
CTTAGCAGTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGATGC1580.1408287504568021No Hit
GTCGTCATTGCGCCAGCTCGTCAGCGCTGGTCGTCTACCAGCTCGATAAT1580.1408287504568021No Hit
CCCTAACTGCGCAGTTAATAATTCTGGCAATTCGTCTCCACACTAGAAGT1550.13815478684053373No Hit
GCAGTTTCCAGAAACGTGTATCACATCTAGGCATGGAATCTTATGCCAGC1550.13815478684053373No Hit
CGCAGACCCGAGCGAAAGCGGCGGTCCTTACAAGTCCGCTCCTCGGGGAG1550.13815478684053373No Hit
CCAGCGGCTAGCATGTGTAGTGCGCCATATCATATCCAAGATAGTGTAGG1530.13637214442968812No Hit
TTCCGCTTTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACA1530.13637214442968812No Hit
ATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGG1520.13548082322426533No Hit
GTCCTTACAAGTCCGCTCCTCGGGGAGCTTGATTGATAATTCTGTATAAG1500.13369818081341972No Hit
GTGTGCTAGCCCGTCGAGACTGAAAAGCTATAACCCGCAGACCCGAGCGA1450.12924157478630574No Hit
CGAGACTGAAAAGCTATAACCCGCAGACCCGAGCGAAAGCGGCGGTCCTT1450.12924157478630574No Hit
CTCAAAAGGGTGTACACCGCCCTTAGACGTCTTGGTATACGGACAACTGA1440.12835025358088292No Hit
CCGATCTTTTCCCTATCTAATTCACCTCCTATTAGGAGCCGATCGTGCTT1430.12745893237546016No Hit
CGAACAACGAAGAGCGACGATGCCCGTTTCAGGTGGTCCTCAGCGTACGG1420.12656761117003734No Hit
CTCGTTCTTTGGTCGCAATCCTGCGTAATCAACGCCGCAACTTTACGTCG1420.12656761117003734No Hit
CCCAGGATGAGTCCCTCAGCGTGACGCAGTAAACTAGTAGCATCCCGATC1400.12478496875919175No Hit
CGGGGATAATTGCGCGGTTTTGACTGCGGAAGGAAGCCAAGGCCCACCTA1390.12389364755376894No Hit
TGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAACCAATGAGAG1380.12300232634834617No Hit
GGGTAAAACGCAAGCACCGGCTGTCGAGTTGTACGGCCGTTCAGCCACGA1380.12300232634834617No Hit
CGCAGTTAATAATTCTGGCAATTCGTCTCCACACTAGAAGTCGACGAACA1370.12211100514292336No Hit
AGACAGAGTCGCTATCGTTATGTCTCCTTCCCGCGGTCAAGGCGAAACCG1370.12211100514292336No Hit
CCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGGGATGAGCATAAA1370.12211100514292336No Hit
ACCAGATGTGTGCTAGCCCGTCGAGACTGAAAAGCTATAACCCGCAGACC1360.12121968393750054No Hit
CCCAGCGGCTAGCATGTGTAGTGCGCCATATCATATCCAAGATAGTGTAG1350.12032836273207777No Hit
CTAGTTCATAAACGAGGGGCGATGCCCGGGCTCAGAATCCAAGGAGCAAA1340.11943704152665496No Hit
CTGAGACCTATCCGAGTTCAGTGCGACCGTACAGCTCTGGAACCCAAAGG1340.11943704152665496No Hit
CGATAATTGGCGCAAGTTGTAGGTCGGGGGCACCACACCACGCTCGCTTG1330.11854572032123216No Hit
CAGCGGCCCTGACCCCGGGGAACAGTCGCATGACGAGGCAGTTTCCAGAA1310.11676307791038656No Hit
CCAGGAGATGTTGGAACTCTACCAATTGGAGCTTTCTTAGCTGTCTTAGC1310.11676307791038656No Hit
CGCGGTTTTGACTGCGGAAGGAAGCCAAGGCCCACCTAAAGTATGCTGCA1300.11587175670496376No Hit
CTCTGGAACCCAAAGGTTCGTTTTTTTCTTGGTACCTATTCCTCCAGGAA1290.11498043549954097No Hit
ATGGAATCTTATGCCAGCTAACGGAACAAGCTTTGTGCCATTCGGACCTA1280.11408911429411817No Hit
CCCTCTTTACAGCAAATACCAGCGGCCCTGACCCCGGGGAACAGTCGCAT1280.11408911429411817No Hit
CAGCAAAGGTTTTTCCAGGAGATGTTGGAACTCTACCAATTGGAGCTTTC1280.11408911429411817No Hit
CCTGAGACAGAGTCGCTATCGTTATGTCTCCTTCCCGCGGTCAAGGCGAA1270.11319779308869539No Hit
CCCGCTTTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAA1270.11319779308869539No Hit
CACTTAGTTACAGTTTTATGGATAATTGGGATATTCTTTGGTATAGTTGG1270.11319779308869539No Hit
CAGGAGATGTTGGAACTCTACCAATTGGAGCTTTCTTAGCTGTCTTAGCA1270.11319779308869539No Hit
GTTTATAAGGAATATATCCCATTTTTAGTTATAATGATGCCTTATGTGAT1230.10963250826700419No Hit
GTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAACCAATGAGA1230.10963250826700419No Hit
CCGGCAGGTTCATTTTAAACGCGGTGACTAGGATGCTCATTTGAATGTCC1220.10874118706158137No Hit
GGATAATTGGGATATTCTTTGGTATAGTTGGGATTTTAATATCATTAATA1220.10874118706158137No Hit
TGAGAATTGGGATACAGGACCCAAAAGGCTGAAAGGGGGCAGTGAAGTCG1200.10695854465073579No Hit
CTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGGGATGAGCATAAAC1190.10606722344531298No Hit
CAGCTCTGGAACCCAAAGGTTCGTTTTTTTCTTGGTACCTATTCCTCCAG1190.10606722344531298No Hit
GGTAAAACGCAAGCACCGGCTGTCGAGTTGTACGGCCGTTCAGCCACGAG1190.10606722344531298No Hit
ACCTAAAGTATGCTGCAAACATATGCAGATCCCCTGAGACAGAGTCGCTA1180.10517590223989019No Hit
GTCGCTATCGTTATGTCTCCTTCCCGCGGTCAAGGCGAAACCGCAGCAAA1170.10428458103446739No Hit
GCCAGCTAACGGAACAAGCTTTGTGCCATTCGGACCTACCGTAAGCCTAT1170.10428458103446739No Hit
GCGGTTTTGACTGCGGAAGGAAGCCAAGGCCCACCTAAAGTATGCTGCAA1160.10339325982904458No Hit
GCGGCCCTGACCCCGGGGAACAGTCGCATGACGAGGCAGTTTCCAGAAAC1160.10339325982904458No Hit
GTTTTATGGATAATTGGGATATTCTTTGGTATAGTTGGGATTTTAATATC1150.1025019386236218No Hit
CGCTATCGTTATGTCTCCTTCCCGCGGTCAAGGCGAAACCGCAGCAAACT1130.10071929621277621No Hit
ATCGAGGTCATGACTGGACACTGCATCGGAAGACACTTCGTGCCGACACG1130.10071929621277621No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)