FastQCFastQC Report
Wed 10 Aug 2022
BORED_1_R2.fq

Summary

[OK]Basic Statistics

MeasureValue
FilenameBORED_1_R2.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences112193
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCT11110.9902578592247289No Hit
CTTAGATATTTTAAAGAGGCATCTATCACATAAGGCATCATTATAACTAA6060.5401406504862157No Hit
CCTAACTTAATGGACGGGAGGTATCCCAATAGGAGGTTTCCTCCTATGGT5670.5053791234747266No Hit
CTTCCATTTCCGCCCAAGCTGCTCACAGTATACGGGCGTCGGCATCCAGA4780.4260515361920975No Hit
CTCGTAACCAAACATGCACAGCGGTCAAACAGTATGTCCCAAGGGGACTT4500.4010945424402592No Hit
CCCTAACTTAATGGACGGGAGGTATCCCAATAGGAGGTTTCCTCCTATGG4370.38950736676976283No Hit
CCCAATTATCCATAAAACTGTAACTAAGTGAGGCTCTCTCATTGGTTTAT4250.37881151230468924No Hit
CTTCACTGCCCCCTTTCAGCCTTTTGGGTCCTGTATCCCAATTCTCAGAG4130.36811565783961564No Hit
CGAATAGCTCGTAACCAAACATGCACAGCGGTCAAACAGTATGTCCCAAG4040.36009376699081047No Hit
CTCTCATTGGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCA4020.3583111245799649No Hit
CATTAATTAGGGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAAT3710.33068016721185817No Hit
CTAACTTAATGGACGGGAGGTATCCCAATAGGAGGTTTCCTCCTATGGTT3650.32533223997932137No Hit
CGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGGCTAGA3570.31820167033593894No Hit
CTGAACTCGGATAGGTCTCAGAAAAACGAAATATAGGCTTACGGTAGGTC3390.3021578886383286No Hit
GCTGCTCACAGTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGG3330.2968099614057918No Hit
CTGAGGACCACCTGAAACGGGCATCGTCGCTCTTCGTTGTTCGTCGACTT3270.291462034173255No Hit
CTCTCTCATTGGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGC3130.2789835372973358No Hit
AAAAAAACGAACCTTTGGGTTCCAGAGCTGTACGGTCGCACTGAACTCGG3100.27630957368106746No Hit
CCTGAAACGGGCATCGTCGCTCTTCGTTGTTCGTCGACTTCTAGTGTGGA2980.26561371921599386No Hit
CAATTATCCATAAAACTGTAACTAAGTGAGGCTCTCTCATTGGTTTATAC2970.26472239801057107No Hit
GGCCGATTGTCGCAGCCCGGGTCGATTATAACAACGGTGCAATCTCAGCT2830.2522439011346519No Hit
CTGTAACTAAGTGAGGCTCTCTCATTGGTTTATACTTCAATATAAGCCTT2810.2504612587238063No Hit
CTAGCGTACGAGCACTATGGTCAGTAATTCCTGGAGGAATAGGTACCAAG2730.2433306890804239No Hit
GGGATAGATAGCCACCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTT2720.2424393678750011No Hit
GGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGGAGGTTTC2670.2379827618478871No Hit
GCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGGAGGTTTCC2660.23709144064246432No Hit
CTTATACAGAATTATCAATCAAGCTCCCCGAGGAGCGGACTTGTAAGGAC2640.23530879823161874No Hit
TCCATTTCCGCCCAAGCTGCTCACAGTATACGGGCGTCGGCATCCAGACC2520.22461294376654514No Hit
CGTGGTTTTACTAGGCTAGACTAGCGTACGAGCACTATGGTCAGTAATTC2480.22104765894485398No Hit
GTCGCACTGAACTCGGATAGGTCTCAGAAAAACGAAATATAGGCTTACGG2410.21480841050689436No Hit
AAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTCTCTCA2400.21391708930147157No Hit
TATAGATTGGCGCTAGTAGATCACAGAACAACCGCCGCATACGGCCGATT2280.20322123483639798No Hit
CCCAATTCTCAGAGGTCCCGCCGTACGCTGAGGACCACCTGAAACGGGCA2280.20322123483639798No Hit
GACGAATTGCCAGAATTATTAACTGCGCAGTTAGGGCAGCGTCTGAGGAA2250.2005472712201296No Hit
GTTTATTGGAAGCATCAACCTGCGCCGTCTTGTTAACTTGTCATATCGCG2230.198764628809284No Hit
GTTGGTTTATGCTCATCCCTACTCATAACCCCAGCACTTAGATATTTTAA2220.19787330760386118No Hit
CTTGGCTTCCTTCCGCAGTCAAAACCGCGCAATTATCCCCGTCCTGATTT2220.19787330760386118No Hit
CTGAAACGGGCATCGTCGCTCTTCGTTGTTCGTCGACTTCTAGTGTGGAG2190.1951993439875928No Hit
CACGTTTCTGGAAACTGCCTCGTCATGCGACTGTTCCCCGGGGTCAGGGC2180.19430802278217002No Hit
ATTAGGGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGGAG2150.19163405916590162No Hit
GAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTCTCTCATTG2120.18896009554963322No Hit
ATTACTTCCATTTCCGCCCAAGCTGCTCACAGTATACGGGCGTCGGCATC2110.1880687743442104No Hit
CGAATTGCCAGAATTATTAACTGCGCAGTTAGGGCAGCGTCTGAGGAAGT2070.18450348952251924No Hit
CCTTGACCGCGGGAAGGAGACATAACGATAGCGACTCTGTCTCAGGGGAT2050.18272084711167363No Hit
TTATAAACTACTGCTAAGACAGCTAAGAAAGCTCCAATTGGTAGAGTTCC2040.18182952590625084No Hit
CCTCGATCCATCGCGTATAGGGACGCCCCCTGCTCGCGTTACTGCCAAGC2040.18182952590625084No Hit
GTCAAAACCGCGCAATTATCCCCGTCCTGATTTACTGGACTCGCAACGTG2010.17915556228998245No Hit
CTCAGCTAAACCGACGCAGTTTTGCTCCTTGGATTCTGAGCCCGGGCATC2000.17826424108455963No Hit
CTCATCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTA1980.17648159867371405No Hit
ATTAATTAGGGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATA1970.17559027746829123No Hit
CTCGGATAGGTCTCAGAAAAACGAAATATAGGCTTACGGTAGGTCCGAAT1960.17469895626286847No Hit
CCAAGCTGCTCACAGTATACGGGCGTCGGCATCCAGACCGTCGGCTGATC1960.17469895626286847No Hit
CACAAAGCTTGTTCCGTTAGCTGGCATAAGATTCCATGCCTAGATGTGAT1920.17113367144117725No Hit
GTGAGGCTCTCTCATTGGTTTATACTTCAATATAAGCCTTGGTAGGGATA1900.16935102903033167No Hit
GGATAGATAGCCACCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTG1900.16935102903033167No Hit
CTTGACCGCGGGAAGGAGACATAACGATAGCGACTCTGTCTCAGGGGATC1860.16578574420864045No Hit
CAAGCTGCTCACAGTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCG1850.16489442300321766No Hit
CTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGGAGGTTTCCT1830.16311178059237205No Hit
CTTTTGGGTCCTGTATCCCAATTCTCAGAGGTCCCGCCGTACGCTGAGGA1830.16311178059237205No Hit
GGGAGGTATCCCAATAGGAGGTTTCCTCCTATGGTTTTCAAAACAATCAC1790.1595464957706809No Hit
CGCGAATTGGGGACATTCAAATGAGCATCCTAGTCACCGCGTTTAAAATG1780.15865517456525807No Hit
CGTCGCTCTTCGTTGTTCGTCGACTTCTAGTGTGGAGACGAATTGCCAGA1740.15508988974356688No Hit
GTTAGGGCAGCGTCTGAGGAAGTTTGCTGCGGTTTCGCCTTGACCGCGGG1730.1541985685381441No Hit
CCCGGATCGATGACGCGAATTGGGGACATTCAAATGAGCATCCTAGTCAC1720.15330724733272127No Hit
CACAGAACAACCGCCGCATACGGCCGATTGTCGCAGCCCGGGTCGATTAT1700.1515246049218757No Hit
TTATAACTAAAAATGGGATATATTCCTTATAAACTACTGCTAAGACAGCT1700.1515246049218757No Hit
GAACAACCGCCGCATACGGCCGATTGTCGCAGCCCGGGTCGATTATAACA1670.1488506413056073No Hit
AGAAAAACGAAATATAGGCTTACGGTAGGTCCGAATGGCACAAAGCTTGT1640.1461766776893389No Hit
GTTTTACTAGGCTAGACTAGCGTACGAGCACTATGGTCAGTAATTCCTGG1640.1461766776893389No Hit
CGTAACCAAACATGCACAGCGGTCAAACAGTATGTCCCAAGGGGACTTAA1640.1461766776893389No Hit
GTCCGAATGGCACAAAGCTTGTTCCGTTAGCTGGCATAAGATTCCATGCC1620.14439403527849332No Hit
CTATCACATAAGGCATCATTATAACTAAAAATGGGATATATTCCTTATAA1620.14439403527849332No Hit
CAAACATGCACAGCGGTCAAACAGTATGTCCCAAGGGGACTTAAGCGCGG1620.14439403527849332No Hit
CGCTGGTATTTGCTGTAAAGAGGGGCGTTGAGTCCGTCCGACTTCACTGC1620.14439403527849332No Hit
GGGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGGAGGTTT1610.1435027140730705No Hit
CAGGAACATCCTCGTGTTAGATATTGAGGCTGCTTCGTGTCGGCACGAAG1580.1408287504568021No Hit
GAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGGAGGTTTCCTCC1580.1408287504568021No Hit
CATAACGATAGCGACTCTGTCTCAGGGGATCTGCATATGTTTGCAGCATA1580.1408287504568021No Hit
CACCCTTTTGAGCAATGATTGCACAACCTGCGATCACCTTATACAGAATT1580.1408287504568021No Hit
AGAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTCTCTCATT1570.13993742925137934No Hit
TTCCATTTCCGCCCAAGCTGCTCACAGTATACGGGCGTCGGCATCCAGAC1560.13904610804595652No Hit
GCCCAAGCTGCTCACAGTATACGGGCGTCGGCATCCAGACCGTCGGCTGA1540.13726346563511094No Hit
GCGGGTTATAGCTTTTCAGTCTCGACGGGCTAGCACACATCTGGTTGACT1540.13726346563511094No Hit
CCTTGGCTTCCTTCCGCAGTCAAAACCGCGCAATTATCCCCGTCCTGATT1530.13637214442968812No Hit
CAGCGGTCAAACAGTATGTCCCAAGGGGACTTAAGCGCGGTGGCCTCCCC1530.13637214442968812No Hit
CATCATTATAACTAAAAATGGGATATATTCCTTATAAACTACTGCTAAGA1500.13369818081341972No Hit
GTTAACTTGTCATATCGCGCACGTAGTAGCCTAGAGCGCCAGGGGCGGAA1500.13369818081341972No Hit
GGGGCGTTGAGTCCGTCCGACTTCACTGCCCCCTTTCAGCCTTTTGGGTC1490.13280685960799693No Hit
GCTGTACGGTCGCACTGAACTCGGATAGGTCTCAGAAAAACGAAATATAG1480.13191553840257414No Hit
GCATATGTTTGCAGCATACTTTAGGTGGGCCTTGGCTTCCTTCCGCAGTC1480.13191553840257414No Hit
ATCACATAAGGCATCATTATAACTAAAAATGGGATATATTCCTTATAAAC1470.13102421719715132No Hit
CTTGGATCAGTGGGAAGTGCTCACGCGCGGAGCCCACTGGGCGAACAGCA1460.13013289599172856No Hit
CGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGGCTAGACTAGCGT1460.13013289599172856No Hit
TATAACTAAAAATGGGATATATTCCTTATAAACTACTGCTAAGACAGCTA1430.12745893237546016No Hit
CTGGTATTTGCTGTAAAGAGGGGCGTTGAGTCCGTCCGACTTCACTGCCC1430.12745893237546016No Hit
CCCAACTATACCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGT1430.12745893237546016No Hit
AGCTGCTCACAGTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTG1420.12656761117003734No Hit
CTCAGAGGTCCCGCCGTACGCTGAGGACCACCTGAAACGGGCATCGTCGC1400.12478496875919175No Hit
CTCGGGTCTGCGGGTTATAGCTTTTCAGTCTCGACGGGCTAGCACACATC1390.12389364755376894No Hit
CTAGGCTAGACTAGCGTACGAGCACTATGGTCAGTAATTCCTGGAGGAAT1380.12300232634834617No Hit
CTCCCCTATCCCCTACGAGGCTACCCGGATCGATGACGCGAATTGGGGAC1380.12300232634834617No Hit
CTCAGAAAAACGAAATATAGGCTTACGGTAGGTCCGAATGGCACAAAGCT1380.12300232634834617No Hit
CCGGGGTCAGGGCCGCTGGTATTTGCTGTAAAGAGGGGCGTTGAGTCCGT1370.12211100514292336No Hit
ATTCCTTATAAACTACTGCTAAGACAGCTAAGAAAGCTCCAATTGGTAGA1370.12211100514292336No Hit
TGGGAGTATTTGCACTTGTGGTAACGGTATTTGCATTATTGATGGTTTTT1360.12121968393750054No Hit
GTCAAACAGTATGTCCCAAGGGGACTTAAGCGCGGTGGCCTCCCCTATCC1350.12032836273207777No Hit
GCCACCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTAT1340.11943704152665496No Hit
CTGATCGTGGTTTTACTAGGCTAGACTAGCGTACGAGCACTATGGTCAGT1320.11765439911580937No Hit
AGAACAACCGCCGCATACGGCCGATTGTCGCAGCCCGGGTCGATTATAAC1320.11765439911580937No Hit
CCAGCACTTAGATATTTTAAAGAGGCATCTATCACATAAGGCATCATTAT1300.11587175670496376No Hit
GGCTAGACTAGCGTACGAGCACTATGGTCAGTAATTCCTGGAGGAATAGG1290.11498043549954097No Hit
GCCGCTGGTATTTGCTGTAAAGAGGGGCGTTGAGTCCGTCCGACTTCACT1290.11498043549954097No Hit
GAAAAACGAAATATAGGCTTACGGTAGGTCCGAATGGCACAAAGCTTGTT1290.11498043549954097No Hit
CTCGTGTTAGATATTGAGGCTGCTTCGTGTCGGCACGAAGTGTCTTCCGA1270.11319779308869539No Hit
CTCATTGGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACC1270.11319779308869539No Hit
GCTTACGGTAGGTCCGAATGGCACAAAGCTTGTTCCGTTAGCTGGCATAA1270.11319779308869539No Hit
CTTACGGTAGGTCCGAATGGCACAAAGCTTGTTCCGTTAGCTGGCATAAG1270.11319779308869539No Hit
CTCACAGTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTT1270.11319779308869539No Hit
CTGGAAACTGCCTCGTCATGCGACTGTTCCCCGGGGTCAGGGCCGCTGGT1260.11230647188327257No Hit
CTTAATGGACGGGAGGTATCCCAATAGGAGGTTTCCTCCTATGGTTTTCA1250.11141515067784977No Hit
CTTTAGGTGGGCCTTGGCTTCCTTCCGCAGTCAAAACCGCGCAATTATCC1240.11052382947242699No Hit
GTTTGCTGCGGTTTCGCCTTGACCGCGGGAAGGAGACATAACGATAGCGA1220.10874118706158137No Hit
GGCTTCCTTCCGCAGTCAAAACCGCGCAATTATCCCCGTCCTGATTTACT1210.10784986585615859No Hit
GATAGATAGCCACCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGT1210.10784986585615859No Hit
CACAGGCGTCATACTTCCCAAGAAGCGGCCATAGCCCAGATGCGAGGTGG1210.10784986585615859No Hit
CTAAAAATGGGATATATTCCTTATAAACTACTGCTAAGACAGCTAAGAAA1190.10606722344531298No Hit
GTCGACTTCTAGTGTGGAGACGAATTGCCAGAATTATTAACTGCGCAGTT1190.10606722344531298No Hit
CCTGTATCCCAATTCTCAGAGGTCCCGCCGTACGCTGAGGACCACCTGAA1190.10606722344531298No Hit
CCCAATAGGAGGTTTCCTCCTATGGTTTTCAAAACAATCACCATCATGCT1180.10517590223989019No Hit
CCGGAAGATACGCTCTAAGCTCGGCAATCGCTCTTGCCGTGCGAGATGCT1180.10517590223989019No Hit
TAGATAGCCACCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTG1180.10517590223989019No Hit
ATTTTAAAGAGGCATCTATCACATAAGGCATCATTATAACTAAAAATGGG1180.10517590223989019No Hit
CTTCTAGTGTGGAGACGAATTGCCAGAATTATTAACTGCGCAGTTAGGGC1180.10517590223989019No Hit
CTTTGCCCGATCCCGGAAGATACGCTCTAAGCTCGGCAATCGCTCTTGCC1170.10428458103446739No Hit
CCTGGAGGAATAGGTACCAAGAAAAAAACGAACCTTTGGGTTCCAGAGCT1170.10428458103446739No Hit
CGAACCTTTGGGTTCCAGAGCTGTACGGTCGCACTGAACTCGGATAGGTC1150.1025019386236218No Hit
GGCTGATCGTGGTTTTACTAGGCTAGACTAGCGTACGAGCACTATGGTCA1140.10161061741819899No Hit
GAAAGATCATGTTTATTGGAAGCATCAACCTGCGCCGTCTTGTTAACTTG1130.10071929621277621No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)