FastQCFastQC Report
Wed 10 Aug 2022
BORED_2_R1.fq

Summary

[OK]Basic Statistics

MeasureValue
FilenameBORED_2_R1.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences137581
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGT10330.7508304198980964No Hit
CGGCTGTCGAGTTGTACGGCCGTTCAGCCACGAGTCACGGGGTCTAACGC8310.6040078208473554No Hit
CTTTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATAT8250.5996467535488185No Hit
GCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGA7760.5640313706107675No Hit
CTGAGACAGAGTCGCTATCGTTATGTCTCCTTCCCGCGGTCAAGGCGAAA7540.5480407905161323No Hit
CGCTTTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAAT7020.5102448739288129No Hit
GTAAAACGCAAGCACCGGCTGTCGAGTTGTACGGCCGTTCAGCCACGAGT7000.5087911848293005No Hit
CCGCTTTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAA6950.5051569620805199No Hit
CTCAGACGCTGCCCTAACTGCGCAGTTAATAATTCTGGCAATTCGTCTCC5760.41866246065953877No Hit
CCCATTTTTAGTTATAATGATGCCTTATGTGATAGATGCCTCTTTAAAAT4990.3626954303283157No Hit
GGAAGCTATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTG4910.35688067393026657No Hit
GTTATGAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAA4610.3350753374375822No Hit
GCCTCACTTAGTTACAGTTTTATGGATAATTGGGATATTCTTTGGTATAG4590.33362164833806995No Hit
GCCATTCGGACCTACCGTAAGCCTATATTTCGTTTTTCTGAGACCTATCC4430.32199213554197165No Hit
CTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGGGATGAGCATAAACCA4340.31545053459416633No Hit
CCGGGATCGGGCAAAGGGGCAACTCGAGCTAATCTCCCCAGCGGCTAGCA4280.3110894672956295No Hit
GTCTCCTTCCCGCGGTCAAGGCGAAACCGCAGCAAACTTCCTCAGACGCT4240.3081820890966049No Hit
GGGATAATTGCGCGGTTTTGACTGCGGAAGGAAGCCAAGGCCCACCTAAA4220.3067283999970926No Hit
GGGGTTATGAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGG4110.29873310994977503No Hit
CTCACTTAGTTACAGTTTTATGGATAATTGGGATATTCTTTGGTATAGTT4050.2943720426512382No Hit
GGACAACTGATGGACCCACGTTGCGAGTCCAGTAAATCAGGACGGGGATA4040.29364519810148204No Hit
CCCGTTTCAGGTGGTCCTCAGCGTACGGCGGGACCTCTGAGAATTGGGAT3990.2900109753527013No Hit
CTTACAAGTCCGCTCCTCGGGGAGCTTGATTGATAATTCTGTATAAGGTG3930.28564990805416446No Hit
GCCCTAACTGCGCAGTTAATAATTCTGGCAATTCGTCTCCACACTAGAAG3930.28564990805416446No Hit
GCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGGGATGAGCATAA3830.278381462556603No Hit
CTTTGGTATAGTTGGGATTTTAATATCATTAATAGCATGATGGTGATTGT3820.2776546180068469No Hit
CGACGAACAACGAAGAGCGACGATGCCCGTTTCAGGTGGTCCTCAGCGTA3820.2776546180068469No Hit
CCGAGTTCAGTGCGACCGTACAGCTCTGGAACCCAAAGGTTCGTTTTTTT3770.27402039525806615No Hit
CTCCTTCCCGCGGTCAAGGCGAAACCGCAGCAAACTTCCTCAGACGCTGC3760.27329355070831No Hit
CGGACAACTGATGGACCCACGTTGCGAGTCCAGTAAATCAGGACGGGGAT3750.27256670615855383No Hit
GTGTACACCGCCCTTAGACGTCTTGGTATACGGACAACTGATGGACCCAC3690.268205638860017No Hit
ATCACATCTAGGCATGGAATCTTATGCCAGCTAACGGAACAAGCTTTGTG3610.26239088246196784No Hit
AGGAGATGTTGGAACTCTACCAATTGGAGCTTTCTTAGCTGTCTTAGCAG3590.2609371933624556No Hit
CTGTGATCTACTAGCGCCAATCTATATTACTCACAATATCCTAAAAACGA3420.2485808360166011No Hit
CGGGGAACAGTCGCATGACGAGGCAGTTTCCAGAAACGTGTATCACATCT3350.24349292416830812No Hit
GGCAGTTTCCAGAAACGTGTATCACATCTAGGCATGGAATCTTATGCCAG3330.24203923506879582No Hit
GGGGATAATTGCGCGGTTTTGACTGCGGAAGGAAGCCAAGGCCCACCTAA3310.24058554596928355No Hit
CCCTGACCCCGGGGAACAGTCGCATGACGAGGCAGTTTCCAGAAACGTGT3200.23259025592196597No Hit
CGCCAATCTATATTACTCACAATATCCTAAAAACGATCAGCCGGCAGGTT3120.22677549952391682No Hit
CTATATTTCGTTTTTCTGAGACCTATCCGAGTTCAGTGCGACCGTACAGC3110.22604865497416068No Hit
CCTGACCCCGGGGAACAGTCGCATGACGAGGCAGTTTCCAGAAACGTGTA3100.22532181042440452No Hit
GTCGAGACTGAAAAGCTATAACCCGCAGACCCGAGCGAAAGCGGCGGTCC3080.22386812132489226No Hit
CCTCACTTAGTTACAGTTTTATGGATAATTGGGATATTCTTTGGTATAGT3060.22241443222537996No Hit
CGCATGACGAGGCAGTTTCCAGAAACGTGTATCACATCTAGGCATGGAAT3050.2216875876756238No Hit
GGATAATTGCGCGGTTTTGACTGCGGAAGGAAGCCAAGGCCCACCTAAAG3050.2216875876756238No Hit
CCAGAATTCAAGACGTTAACAGTTCTTGGCGCAAATAGCGCTGAATCGCT3040.22096074312586764No Hit
ATTTTTAGTTATAATGATGCCTTATGTGATAGATGCCTCTTTAAAATATC3020.2195070540263554No Hit
GTCGGGGGCACCACACCACGCTCGCTTGGCAGTAACGCGAGCAGGGGGCG3000.21805336492684307No Hit
CCGGGGAACAGTCGCATGACGAGGCAGTTTCCAGAAACGTGTATCACATC2960.2151459867278185No Hit
GCCCGTTTCAGGTGGTCCTCAGCGTACGGCGGGACCTCTGAGAATTGGGA2940.21369229762830624No Hit
CTGGGGTTATGAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGAT2800.20351647393172023No Hit
GGATATTCTTTGGTATAGTTGGGATTTTAATATCATTAATAGCATGATGG2780.20206278483220794No Hit
GGGGAACAGTCGCATGACGAGGCAGTTTCCAGAAACGTGTATCACATCTA2770.20133594028245178No Hit
CTGCGATAGGCTAGTTCATAAACGAGGGGCGATGCCCGGGCTCAGAATCC2760.20060909573269564No Hit
GTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGGGA2760.20060909573269564No Hit
GGGATATTCTTTGGTATAGTTGGGATTTTAATATCATTAATAGCATGATG2740.19915540663318337No Hit
CTTTACAGCAAATACCAGCGGCCCTGACCCCGGGGAACAGTCGCATGACG2710.19697487298391494No Hit
GTCGAGTTGTACGGCCGTTCAGCCACGAGTCACGGGGTCTAACGCCTACC2700.1962480284341588No Hit
GGCAGGTTCATTTTAAACGCGGTGACTAGGATGCTCATTTGAATGTCCCC2670.19406749478489035No Hit
TGCCATTCGGACCTACCGTAAGCCTATATTTCGTTTTTCTGAGACCTATC2630.19116011658586576No Hit
GTCGGTTTAGCTGAGATTGCACCGTTGTTATAATCGACCCGGGCTGCGAC2620.19043327203610963No Hit
CTCGGCAATAGGATTGTAGCCCAGGATGAGTCCCTCAGCGTGACGCAGTA2620.19043327203610963No Hit
CTGACCCCGGGGAACAGTCGCATGACGAGGCAGTTTCCAGAAACGTGTAT2590.1882527383868412No Hit
GTTTTTCTGAGACCTATCCGAGTTCAGTGCGACCGTACAGCTCTGGAACC2540.18461851563806048No Hit
CCTCTTTACAGCAAATACCAGCGGCCCTGACCCCGGGGAACAGTCGCATG2530.18389167108830434No Hit
GTCTGCGATAGGCTAGTTCATAAACGAGGGGCGATGCCCGGGCTCAGAAT2500.1817111374390359No Hit
ATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACCGC2500.1817111374390359No Hit
GTCGTCATTGCGCCAGCTCGTCAGCGCTGGTCGTCTACCAGCTCGATAAT2460.17880375924001135No Hit
CGGCAGGTTCATTTTAAACGCGGTGACTAGGATGCTCATTTGAATGTCCC2420.17589638104098676No Hit
GTGCTAGCCCGTCGAGACTGAAAAGCTATAACCCGCAGACCCGAGCGAAA2370.17226215829220604No Hit
CGGCAATAGGATTGTAGCCCAGGATGAGTCCCTCAGCGTGACGCAGTAAA2370.17226215829220604No Hit
CTTAGTTACAGTTTTATGGATAATTGGGATATTCTTTGGTATAGTTGGGA2360.1715353137424499No Hit
GGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGAGATGT2300.16717424644391304No Hit
CCCCTCTTTACAGCAAATACCAGCGGCCCTGACCCCGGGGAACAGTCGCA2290.1664474018941569No Hit
CTCAGCGTGACGCAGTAAACTAGTAGCATCCCGATCTTTTCCCTATCTAA2280.16572055734440075No Hit
TTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTA2280.16572055734440075No Hit
GTTGATACCGTCTGCGATAGGCTAGTTCATAAACGAGGGGCGATGCCCGG2270.16499371279464461No Hit
CCTGTTGATACCGTCTGCGATAGGCTAGTTCATAAACGAGGGGCGATGCC2250.16354002369513232No Hit
GGCAATTCGTCTCCACACTAGAAGTCGACGAACAACGAAGAGCGACGATG2210.16063264549610773No Hit
CTTGGTATACGGACAACTGATGGACCCACGTTGCGAGTCCAGTAAATCAG2170.15772526729708317No Hit
ATTCGGACCTACCGTAAGCCTATATTTCGTTTTTCTGAGACCTATCCGAG2140.15554473364781474No Hit
GTTTTTTTCTTGGTACCTATTCCTCCAGGAATTACTGACCATAGTGCTCG2120.15409104454830244No Hit
GTCCTTACAAGTCCGCTCCTCGGGGAGCTTGATTGATAATTCTGTATAAG2090.151910510899034No Hit
CTCAAAAGGGTGTACACCGCCCTTAGACGTCTTGGTATACGGACAACTGA2080.15118366634927788No Hit
GCAGTTTCCAGAAACGTGTATCACATCTAGGCATGGAATCTTATGCCAGC2060.1497299772497656No Hit
CGAGGATGTTCCTGTTGATACCGTCTGCGATAGGCTAGTTCATAAACGAG2050.14900313270000945No Hit
CCCACCTAAAGTATGCTGCAAACATATGCAGATCCCCTGAGACAGAGTCG2050.14900313270000945No Hit
CTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTT2040.1482762881502533No Hit
ATCTAATTCACCTCCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAA2040.1482762881502533No Hit
CTCTTTACAGCAAATACCAGCGGCCCTGACCCCGGGGAACAGTCGCATGA2040.1482762881502533No Hit
CGCATATAACCACATTCAAATTGACCTCCCTCAGGAAGCTAAGAAATACT2000.14536890995122873No Hit
CCACATTCAAATTGACCTCCCTCAGGAAGCTAAGAAATACTATCTCGGCA1990.1446420654014726No Hit
CGCAAGTTGTAGGTCGGGGGCACCACACCACGCTCGCTTGGCAGTAACGC1940.14100784265269187No Hit
GTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGATGCCTTATGT1930.14028099810293573No Hit
CCCAGGATGAGTCCCTCAGCGTGACGCAGTAAACTAGTAGCATCCCGATC1910.13882730900342344No Hit
CCGCAGACCCGAGCGAAAGCGGCGGTCCTTACAAGTCCGCTCCTCGGGGA1910.13882730900342344No Hit
GTGTGCTAGCCCGTCGAGACTGAAAAGCTATAACCCGCAGACCCGAGCGA1880.136646775354155No Hit
GCCAGCTAACGGAACAAGCTTTGTGCCATTCGGACCTACCGTAAGCCTAT1880.136646775354155No Hit
ATGGAATCTTATGCCAGCTAACGGAACAAGCTTTGTGCCATTCGGACCTA1860.1351930862546427No Hit
TGGCAATTCGTCTCCACACTAGAAGTCGACGAACAACGAAGAGCGACGAT1840.13373939715513045No Hit
CGAACAACGAAGAGCGACGATGCCCGTTTCAGGTGGTCCTCAGCGTACGG1840.13373939715513045No Hit
CAGCGGCCCTGACCCCGGGGAACAGTCGCATGACGAGGCAGTTTCCAGAA1840.13373939715513045No Hit
GTTGATTTCATGAATGCGATTTCTGATATGGCGGCGGTCGGTTTCCAGTT1830.13301255260537428No Hit
AGACAGAGTCGCTATCGTTATGTCTCCTTCCCGCGGTCAAGGCGAAACCG1830.13301255260537428No Hit
GCGGTTTTGACTGCGGAAGGAAGCCAAGGCCCACCTAAAGTATGCTGCAA1780.12937832985659356No Hit
CCAGGAGATGTTGGAACTCTACCAATTGGAGCTTTCTTAGCTGTCTTAGC1770.12865148530683743No Hit
GGTAAAACGCAAGCACCGGCTGTCGAGTTGTACGGCCGTTCAGCCACGAG1770.12865148530683743No Hit
CACTTAGTTACAGTTTTATGGATAATTGGGATATTCTTTGGTATAGTTGG1750.12719779620732513No Hit
CGAGACTGAAAAGCTATAACCCGCAGACCCGAGCGAAAGCGGCGGTCCTT1750.12719779620732513No Hit
CGATAATTGGCGCAAGTTGTAGGTCGGGGGCACCACACCACGCTCGCTTG1690.12283672890878827No Hit
CTCGTTCTTTGGTCGCAATCCTGCGTAATCAACGCCGCAACTTTACGTCG1680.12210988435903214No Hit
CGCGGTTTTGACTGCGGAAGGAAGCCAAGGCCCACCTAAAGTATGCTGCA1670.12138303980927599No Hit
CCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGGGATGAGCATAAA1660.12065619525951986No Hit
CCCTAACTGCGCAGTTAATAATTCTGGCAATTCGTCTCCACACTAGAAGT1650.11992935070976371No Hit
GGGTAAAACGCAAGCACCGGCTGTCGAGTTGTACGGCCGTTCAGCCACGA1650.11992935070976371No Hit
CTTAGCAGTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGATGC1650.11992935070976371No Hit
GTTGGAACTCTACCAATTGGAGCTTTCTTAGCTGTCTTAGCAGTAGTTTA1640.11920250616000755No Hit
ACCAGATGTGTGCTAGCCCGTCGAGACTGAAAAGCTATAACCCGCAGACC1640.11920250616000755No Hit
CGGCGCTCTTCCTAGTTGGTACAGAACTGAGTGTCATGTGTCCAAAGTTA1640.11920250616000755No Hit
CCCAGCGGCTAGCATGTGTAGTGCGCCATATCATATCCAAGATAGTGTAG1640.11920250616000755No Hit
TGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAACCAATGAGAG1610.11702197251073913No Hit
CCTGAGACAGAGTCGCTATCGTTATGTCTCCTTCCCGCGGTCAAGGCGAA1610.11702197251073913No Hit
GGATAATTGGGATATTCTTTGGTATAGTTGGGATTTTAATATCATTAATA1600.11629512796098299No Hit
GTTTTATGGATAATTGGGATATTCTTTGGTATAGTTGGGATTTTAATATC1580.1148414388614707No Hit
CTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGGGATGAGCATAAAC1560.11338774976195841No Hit
CCGATCTTTTCCCTATCTAATTCACCTCCTATTAGGAGCCGATCGTGCTT1560.11338774976195841No Hit
GCGGGACCTCTGAGAATTGGGATACAGGACCCAAAAGGCTGAAAGGGGGC1560.11338774976195841No Hit
CTAGTTCATAAACGAGGGGCGATGCCCGGGCTCAGAATCCAAGGAGCAAA1530.11120721611268998No Hit
ATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGG1520.11048037156293382No Hit
CTGAGACCTATCCGAGTTCAGTGCGACCGTACAGCTCTGGAACCCAAAGG1510.1097535270131777No Hit
CTACCGTAAGCCTATATTTCGTTTTTCTGAGACCTATCCGAGTTCAGTGC1500.10902668246342154No Hit
CGCAGTTAATAATTCTGGCAATTCGTCTCCACACTAGAAGTCGACGAACA1490.1082998379136654No Hit
GTTTATAAGGAATATATCCCATTTTTAGTTATAATGATGCCTTATGTGAT1480.10757299336390926No Hit
CTCTGGAACCCAAAGGTTCGTTTTTTTCTTGGTACCTATTCCTCCAGGAA1470.10684614881415312No Hit
CGCAGACCCGAGCGAAAGCGGCGGTCCTTACAAGTCCGCTCCTCGGGGAG1470.10684614881415312No Hit
CCAGCGGCTAGCATGTGTAGTGCGCCATATCATATCCAAGATAGTGTAGG1450.10539245971464081No Hit
GCTTGATTGATAATTCTGTATAAGGTGATCGCAGGTTGTGCAATCATTGC1430.10393877061512853No Hit
GTACACCGCCCTTAGACGTCTTGGTATACGGACAACTGATGGACCCACGT1420.10321192606537241No Hit
GTGCCATTCGGACCTACCGTAAGCCTATATTTCGTTTTTCTGAGACCTAT1410.10248508151561625No Hit
GTCGGAAGATCCTGAATAAATCCTACTGTATCTGAAAGAAGAACACTGTA1410.10248508151561625No Hit
CCACACTAGAAGTCGACGAACAACGAAGAGCGACGATGCCCGTTTCAGGT1400.10175823696586012No Hit
CAGCTCTGGAACCCAAAGGTTCGTTTTTTTCTTGGTACCTATTCCTCCAG1390.10103139241610397No Hit
CGGGGATAATTGCGCGGTTTTGACTGCGGAAGGAAGCCAAGGCCCACCTA1380.10030454786634782No Hit
ATCGAGGTCATGACTGGACACTGCATCGGAAGACACTTCGTGCCGACACG1380.10030454786634782No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)