FastQCFastQC Report
Wed 10 Aug 2022
BORED_2_R2.fq

Summary

[OK]Basic Statistics

MeasureValue
FilenameBORED_2_R2.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences137581
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCT14111.0255776597059185No Hit
CCTAACTTAATGGACGGGAGGTATCCCAATAGGAGGTTTCCTCCTATGGT6040.4390141080527108No Hit
CTCGTAACCAAACATGCACAGCGGTCAAACAGTATGTCCCAAGGGGACTT5660.4113940151619773No Hit
CTTCCATTTCCGCCCAAGCTGCTCACAGTATACGGGCGTCGGCATCCAGA5530.40194503601514747No Hit
CCCTAACTTAATGGACGGGAGGTATCCCAATAGGAGGTTTCCTCCTATGG5400.3924960568683176No Hit
CTCTCATTGGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCA5260.3823202331717315No Hit
CTTAGATATTTTAAAGAGGCATCTATCACATAAGGCATCATTATAACTAA5170.37577863222392627No Hit
CCCAATTATCCATAAAACTGTAACTAAGTGAGGCTCTCTCATTGGTTTAT4820.35033907298246125No Hit
CTAACTTAATGGACGGGAGGTATCCCAATAGGAGGTTTCCTCCTATGGTT4730.34379747203465594No Hit
GCTGCTCACAGTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGG4340.31545053459416633No Hit
CTTCACTGCCCCCTTTCAGCCTTTTGGGTCCTGTATCCCAATTCTCAGAG4260.3096357781961172No Hit
CGAATAGCTCGTAACCAAACATGCACAGCGGTCAAACAGTATGTCCCAAG4250.3089089336463611No Hit
CATTAATTAGGGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAAT4220.3067283999970926No Hit
CGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGGCTAGA4210.3060015554473365No Hit
CTGAACTCGGATAGGTCTCAGAAAAACGAAATATAGGCTTACGGTAGGTC4110.29873310994977503No Hit
CTGAGGACCACCTGAAACGGGCATCGTCGCTCTTCGTTGTTCGTCGACTT3850.2798351516561153No Hit
CCTGAAACGGGCATCGTCGCTCTTCGTTGTTCGTCGACTTCTAGTGTGGA3820.2776546180068469No Hit
GGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGGAGGTTTC3750.27256670615855383No Hit
CAATTATCCATAAAACTGTAACTAAGTGAGGCTCTCTCATTGGTTTATAC3720.2703861725092854No Hit
GGCCGATTGTCGCAGCCCGGGTCGATTATAACAACGGTGCAATCTCAGCT3660.26602510521074857No Hit
AAAAAAACGAACCTTTGGGTTCCAGAGCTGTACGGTCGCACTGAACTCGG3530.2565761260639187No Hit
GCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGGAGGTTTCC3490.25366874786489413No Hit
GGGATAGATAGCCACCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTT3470.2522150587653818No Hit
CTAGCGTACGAGCACTATGGTCAGTAATTCCTGGAGGAATAGGTACCAAG3380.24567345781757655No Hit
GTCGCACTGAACTCGGATAGGTCTCAGAAAAACGAAATATAGGCTTACGG3230.23477078957123437No Hit
CCTTGACCGCGGGAAGGAGACATAACGATAGCGACTCTGTCTCAGGGGAT3170.23040972227269754No Hit
TATAGATTGGCGCTAGTAGATCACAGAACAACCGCCGCATACGGCCGATT3110.22604865497416068No Hit
GACGAATTGCCAGAATTATTAACTGCGCAGTTAGGGCAGCGTCTGAGGAA3110.22604865497416068No Hit
AAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTCTCTCA3090.22459496587464836No Hit
CGTGGTTTTACTAGGCTAGACTAGCGTACGAGCACTATGGTCAGTAATTC3070.22314127677513612No Hit
CTGTAACTAAGTGAGGCTCTCTCATTGGTTTATACTTCAATATAAGCCTT3060.22241443222537996No Hit
CTCTCTCATTGGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGC3050.2216875876756238No Hit
CTTATACAGAATTATCAATCAAGCTCCCCGAGGAGCGGACTTGTAAGGAC3020.2195070540263554No Hit
GAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTCTCTCATTG3020.2195070540263554No Hit
CCCAATTCTCAGAGGTCCCGCCGTACGCTGAGGACCACCTGAAACGGGCA3000.21805336492684307No Hit
ATTACTTCCATTTCCGCCCAAGCTGCTCACAGTATACGGGCGTCGGCATC2880.20933123032976939No Hit
ATTAGGGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGGAG2860.20787754123025706No Hit
CACAAAGCTTGTTCCGTTAGCTGGCATAAGATTCCATGCCTAGATGTGAT2780.20206278483220794No Hit
CCTCGATCCATCGCGTATAGGGACGCCCCCTGCTCGCGTTACTGCCAAGC2770.20133594028245178No Hit
GTTAGGGCAGCGTCTGAGGAAGTTTGCTGCGGTTTCGCCTTGACCGCGGG2720.19770171753367105No Hit
GTCAAAACCGCGCAATTATCCCCGTCCTGATTTACTGGACTCGCAACGTG2700.1962480284341588No Hit
AGAAAAACGAAATATAGGCTTACGGTAGGTCCGAATGGCACAAAGCTTGT2680.1947943393346465No Hit
CTTGGCTTCCTTCCGCAGTCAAAACCGCGCAATTATCCCCGTCCTGATTT2660.19334065023513422No Hit
ATTAATTAGGGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATA2600.18897958293659736No Hit
CTTTTGGGTCCTGTATCCCAATTCTCAGAGGTCCCGCCGTACGCTGAGGA2600.18897958293659736No Hit
GTGAGGCTCTCTCATTGGTTTATACTTCAATATAAGCCTTGGTAGGGATA2590.1882527383868412No Hit
GTTGGTTTATGCTCATCCCTACTCATAACCCCAGCACTTAGATATTTTAA2540.18461851563806048No Hit
CTTGACCGCGGGAAGGAGACATAACGATAGCGACTCTGTCTCAGGGGATC2540.18461851563806048No Hit
GTTTATTGGAAGCATCAACCTGCGCCGTCTTGTTAACTTGTCATATCGCG2530.18389167108830434No Hit
GGATAGATAGCCACCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTG2500.1817111374390359No Hit
CACGTTTCTGGAAACTGCCTCGTCATGCGACTGTTCCCCGGGGTCAGGGC2490.18098429288927975No Hit
CGCGAATTGGGGACATTCAAATGAGCATCCTAGTCACCGCGTTTAAAATG2470.17953060378976748No Hit
GTTTTACTAGGCTAGACTAGCGTACGAGCACTATGGTCAGTAATTCCTGG2450.1780769146902552No Hit
GGGAGGTATCCCAATAGGAGGTTTCCTCCTATGGTTTTCAAAACAATCAC2430.17662322559074292No Hit
CTCAGCTAAACCGACGCAGTTTTGCTCCTTGGATTCTGAGCCCGGGCATC2390.17371584739171833No Hit
CTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGGAGGTTTCCT2380.1729890028419622No Hit
GCTGTACGGTCGCACTGAACTCGGATAGGTCTCAGAAAAACGAAATATAG2360.1715353137424499No Hit
GAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGGAGGTTTCCTCC2350.17080846919269374No Hit
TCCATTTCCGCCCAAGCTGCTCACAGTATACGGGCGTCGGCATCCAGACC2280.16572055734440075No Hit
CGAATTGCCAGAATTATTAACTGCGCAGTTAGGGCAGCGTCTGAGGAAGT2280.16572055734440075No Hit
GAACAACCGCCGCATACGGCCGATTGTCGCAGCCCGGGTCGATTATAACA2270.16499371279464461No Hit
GGGGCGTTGAGTCCGTCCGACTTCACTGCCCCCTTTCAGCCTTTTGGGTC2200.15990580094635162No Hit
CTCGGATAGGTCTCAGAAAAACGAAATATAGGCTTACGGTAGGTCCGAAT2150.1562715781975709No Hit
CTGAAACGGGCATCGTCGCTCTTCGTTGTTCGTCGACTTCTAGTGTGGAG2130.1548178890980586No Hit
CGTCGCTCTTCGTTGTTCGTCGACTTCTAGTGTGGAGACGAATTGCCAGA2120.15409104454830244No Hit
CATAACGATAGCGACTCTGTCTCAGGGGATCTGCATATGTTTGCAGCATA2100.15263735544879017No Hit
GCCCAAGCTGCTCACAGTATACGGGCGTCGGCATCCAGACCGTCGGCTGA2100.15263735544879017No Hit
CCAAGCTGCTCACAGTATACGGGCGTCGGCATCCAGACCGTCGGCTGATC2040.1482762881502533No Hit
CTCATCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTA2020.14682259905074102No Hit
CAAACATGCACAGCGGTCAAACAGTATGTCCCAAGGGGACTTAAGCGCGG2000.14536890995122873No Hit
TTATAAACTACTGCTAAGACAGCTAAGAAAGCTCCAATTGGTAGAGTTCC1980.14391522085171643No Hit
CACAGAACAACCGCCGCATACGGCCGATTGTCGCAGCCCGGGTCGATTAT1970.1431883763019603No Hit
GCGGGTTATAGCTTTTCAGTCTCGACGGGCTAGCACACATCTGGTTGACT1970.1431883763019603No Hit
AGAACAACCGCCGCATACGGCCGATTGTCGCAGCCCGGGTCGATTATAAC1930.14028099810293573No Hit
GCCACCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTAT1930.14028099810293573No Hit
CGCTGGTATTTGCTGTAAAGAGGGGCGTTGAGTCCGTCCGACTTCACTGC1900.13810046445366728No Hit
CGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGGCTAGACTAGCGT1890.13737361990391114No Hit
CAGCGGTCAAACAGTATGTCCCAAGGGGACTTAAGCGCGGTGGCCTCCCC1890.13737361990391114No Hit
GTCAAACAGTATGTCCCAAGGGGACTTAAGCGCGGTGGCCTCCCCTATCC1880.136646775354155No Hit
AGAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTCTCTCATT1880.136646775354155No Hit
GCATATGTTTGCAGCATACTTTAGGTGGGCCTTGGCTTCCTTCCGCAGTC1870.13591993080439888No Hit
CTTCTAGTGTGGAGACGAATTGCCAGAATTATTAACTGCGCAGTTAGGGC1870.13591993080439888No Hit
GTCCGAATGGCACAAAGCTTGTTCCGTTAGCTGGCATAAGATTCCATGCC1860.1351930862546427No Hit
CAAGCTGCTCACAGTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCG1860.1351930862546427No Hit
GGCTAGACTAGCGTACGAGCACTATGGTCAGTAATTCCTGGAGGAATAGG1860.1351930862546427No Hit
GTTAACTTGTCATATCGCGCACGTAGTAGCCTAGAGCGCCAGGGGCGGAA1860.1351930862546427No Hit
CTTACGGTAGGTCCGAATGGCACAAAGCTTGTTCCGTTAGCTGGCATAAG1850.13446624170488655No Hit
CTCAGAAAAACGAAATATAGGCTTACGGTAGGTCCGAATGGCACAAAGCT1850.13446624170488655No Hit
CGTAACCAAACATGCACAGCGGTCAAACAGTATGTCCCAAGGGGACTTAA1780.12937832985659356No Hit
CTATCACATAAGGCATCATTATAACTAAAAATGGGATATATTCCTTATAA1770.12865148530683743No Hit
GAAAAACGAAATATAGGCTTACGGTAGGTCCGAATGGCACAAAGCTTGTT1770.12865148530683743No Hit
GGGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGGAGGTTT1770.12865148530683743No Hit
CAGGAACATCCTCGTGTTAGATATTGAGGCTGCTTCGTGTCGGCACGAAG1760.12792464075708126No Hit
CTTTAGGTGGGCCTTGGCTTCCTTCCGCAGTCAAAACCGCGCAATTATCC1750.12719779620732513No Hit
CCTTGGCTTCCTTCCGCAGTCAAAACCGCGCAATTATCCCCGTCCTGATT1730.12574410710781286No Hit
GATAGATAGCCACCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGT1730.12574410710781286No Hit
ATTCCTTATAAACTACTGCTAAGACAGCTAAGAAAGCTCCAATTGGTAGA1720.1250172625580567No Hit
CACCCTTTTGAGCAATGATTGCACAACCTGCGATCACCTTATACAGAATT1720.1250172625580567No Hit
TATAACTAAAAATGGGATATATTCCTTATAAACTACTGCTAAGACAGCTA1680.12210988435903214No Hit
CTGGTATTTGCTGTAAAGAGGGGCGTTGAGTCCGTCCGACTTCACTGCCC1680.12210988435903214No Hit
GCTTACGGTAGGTCCGAATGGCACAAAGCTTGTTCCGTTAGCTGGCATAA1680.12210988435903214No Hit
GCCGCTGGTATTTGCTGTAAAGAGGGGCGTTGAGTCCGTCCGACTTCACT1660.12065619525951986No Hit
CCTGTATCCCAATTCTCAGAGGTCCCGCCGTACGCTGAGGACCACCTGAA1630.11847566161025141No Hit
CTAGCCTATCGCAGACGGTATCAACAGGAACATCCTCGTGTTAGATATTG1620.11774881706049527No Hit
AATTAGGGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGGA1620.11774881706049527No Hit
CTCATTGGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACC1600.11629512796098299No Hit
CCCGGATCGATGACGCGAATTGGGGACATTCAAATGAGCATCCTAGTCAC1600.11629512796098299No Hit
CGAACCTTTGGGTTCCAGAGCTGTACGGTCGCACTGAACTCGGATAGGTC1590.11556828341122685No Hit
TTCCATTTCCGCCCAAGCTGCTCACAGTATACGGGCGTCGGCATCCAGAC1590.11556828341122685No Hit
CTTGGATCAGTGGGAAGTGCTCACGCGCGGAGCCCACTGGGCGAACAGCA1570.11411459431171454No Hit
CTAGGCTAGACTAGCGTACGAGCACTATGGTCAGTAATTCCTGGAGGAAT1570.11411459431171454No Hit
CTCGGGTCTGCGGGTTATAGCTTTTCAGTCTCGACGGGCTAGCACACATC1560.11338774976195841No Hit
CTTAATGGACGGGAGGTATCCCAATAGGAGGTTTCCTCCTATGGTTTTCA1550.11266090521220226No Hit
CCAGCACTTAGATATTTTAAAGAGGCATCTATCACATAAGGCATCATTAT1540.11193406066244613No Hit
CCTGGAGGAATAGGTACCAAGAAAAAAACGAACCTTTGGGTTCCAGAGCT1540.11193406066244613No Hit
TAAAACTGTAACTAAGTGAGGCTCTCTCATTGGTTTATACTTCAATATAA1530.11120721611268998No Hit
CTCAGAGGTCCCGCCGTACGCTGAGGACCACCTGAAACGGGCATCGTCGC1520.11048037156293382No Hit
TTATAACTAAAAATGGGATATATTCCTTATAAACTACTGCTAAGACAGCT1510.1097535270131777No Hit
TGGGAGTATTTGCACTTGTGGTAACGGTATTTGCATTATTGATGGTTTTT1500.10902668246342154No Hit
AAAACGAATAGCTCGTAACCAAACATGCACAGCGGTCAAACAGTATGTCC1490.1082998379136654No Hit
CGGGAGGTATCCCAATAGGAGGTTTCCTCCTATGGTTTTCAAAACAATCA1480.10757299336390926No Hit
CCGGGGTCAGGGCCGCTGGTATTTGCTGTAAAGAGGGGCGTTGAGTCCGT1470.10684614881415312No Hit
GGAACATCCTCGTGTTAGATATTGAGGCTGCTTCGTGTCGGCACGAAGTG1460.10611930426439697No Hit
GCTCGAGTTGCCCCTTTGCCCGATCCCGGAAGATACGCTCTAAGCTCGGC1460.10611930426439697No Hit
CGGAAGATACGCTCTAAGCTCGGCAATCGCTCTTGCCGTGCGAGATGCTA1450.10539245971464081No Hit
CAGCGTCTGAGGAAGTTTGCTGCGGTTTCGCCTTGACCGCGGGAAGGAGA1450.10539245971464081No Hit
TAGGGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGGAGGT1450.10539245971464081No Hit
GGCCGCTGGTATTTGCTGTAAAGAGGGGCGTTGAGTCCGTCCGACTTCAC1440.10466561516488469No Hit
CTGATCGTGGTTTTACTAGGCTAGACTAGCGTACGAGCACTATGGTCAGT1430.10393877061512853No Hit
GAAAGATCATGTTTATTGGAAGCATCAACCTGCGCCGTCTTGTTAACTTG1430.10393877061512853No Hit
CCCAAGGGGACTTAAGCGCGGTGGCCTCCCCTATCCCCTACGAGGCTACC1410.10248508151561625No Hit
CTGCTCACAGTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGT1410.10248508151561625No Hit
AGCTGCTCACAGTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTG1400.10175823696586012No Hit
CAGAGCTGTACGGTCGCACTGAACTCGGATAGGTCTCAGAAAAACGAAAT1400.10175823696586012No Hit
CAATTCTCAGAGGTCCCGCCGTACGCTGAGGACCACCTGAAACGGGCATC1390.10103139241610397No Hit
GTTTGCTGCGGTTTCGCCTTGACCGCGGGAAGGAGACATAACGATAGCGA1390.10103139241610397No Hit
GCCTTTTGGGTCCTGTATCCCAATTCTCAGAGGTCCCGCCGTACGCTGAG1390.10103139241610397No Hit
CACAGTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTAC1380.10030454786634782No Hit
CACAGGCGTCATACTTCCCAAGAAGCGGCCATAGCCCAGATGCGAGGTGG1380.10030454786634782No Hit
GAGGAAGTTTGCTGCGGTTTCGCCTTGACCGCGGGAAGGAGACATAACGA1380.10030454786634782No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)