FastQCFastQC Report
Wed 10 Aug 2022
BORED_3_R1.fq

Summary

[OK]Basic Statistics

MeasureValue
FilenameBORED_3_R1.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences123093
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATAT8740.7100322520370776No Hit
CTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGT8630.7010959193455355No Hit
CCGCTTTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAA7690.6247308945269024No Hit
CGCTTTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAAT7600.6174193495974588No Hit
CGGCTGTCGAGTTGTACGGCCGTTCAGCCACGAGTCACGGGGTCTAACGC7550.6133573801922124No Hit
CTGAGACAGAGTCGCTATCGTTATGTCTCCTTCCCGCGGTCAAGGCGAAA6990.5678633228534523No Hit
GCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGA6930.5629889595671566No Hit
GTAAAACGCAAGCACCGGCTGTCGAGTTGTACGGCCGTTCAGCCACGAGT6300.5118081450610514No Hit
CTCAGACGCTGCCCTAACTGCGCAGTTAATAATTCTGGCAATTCGTCTCC5550.4508786039823548No Hit
GGAAGCTATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTG5340.4338183324803197No Hit
GTTATGAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAA4140.3363310667544052No Hit
GCCATTCGGACCTACCGTAAGCCTATATTTCGTTTTTCTGAGACCTATCC3920.31845840137132087No Hit
CCCATTTTTAGTTATAATGATGCCTTATGTGATAGATGCCTCTTTAAAAT3890.316021219728173No Hit
CCCGTTTCAGGTGGTCCTCAGCGTACGGCGGGACCTCTGAGAATTGGGAT3870.3143964319660744No Hit
CTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGGGATGAGCATAAACCA3850.31277164420397585No Hit
GCCTCACTTAGTTACAGTTTTATGGATAATTGGGATATTCTTTGGTATAG3830.3111468564418773No Hit
CTCCTTCCCGCGGTCAAGGCGAAACCGCAGCAAACTTCCTCAGACGCTGC3800.3087096747987294No Hit
CCGGGATCGGGCAAAGGGGCAACTCGAGCTAATCTCCCCAGCGGCTAGCA3670.29814855434508863No Hit
GGGATAATTGCGCGGTTTTGACTGCGGAAGGAAGCCAAGGCCCACCTAAA3560.2892122216535465No Hit
GTCTCCTTCCCGCGGTCAAGGCGAAACCGCAGCAAACTTCCTCAGACGCT3560.2892122216535465No Hit
CTTACAAGTCCGCTCCTCGGGGAGCTTGATTGATAATTCTGTATAAGGTG3520.28596264612934935No Hit
CTTTGGTATAGTTGGGATTTTAATATCATTAATAGCATGATGGTGATTGT3410.2770263134378072No Hit
AGGAGATGTTGGAACTCTACCAATTGGAGCTTTCTTAGCTGTCTTAGCAG3410.2770263134378072No Hit
CCTGACCCCGGGGAACAGTCGCATGACGAGGCAGTTTCCAGAAACGTGTA3360.27296434403256076No Hit
ATCACATCTAGGCATGGAATCTTATGCCAGCTAACGGAACAAGCTTTGTG3330.2705271623894129No Hit
GCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGGGATGAGCATAA3320.2697147685083636No Hit
GCCCTAACTGCGCAGTTAATAATTCTGGCAATTCGTCTCCACACTAGAAG3310.2689023746273143No Hit
CTGTGATCTACTAGCGCCAATCTATATTACTCACAATATCCTAAAAACGA3300.268089980746265No Hit
GGACAACTGATGGACCCACGTTGCGAGTCCAGTAAATCAGGACGGGGATA3290.26727758686521574No Hit
GGGGTTATGAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGG3260.26484040522206787No Hit
GTGTACACCGCCCTTAGACGTCTTGGTATACGGACAACTGATGGACCCAC3240.2632156174599693No Hit
CGACGAACAACGAAGAGCGACGATGCCCGTTTCAGGTGGTCCTCAGCGTA3120.25346689088737784No Hit
GTCGAGACTGAAAAGCTATAACCCGCAGACCCGAGCGAAAGCGGCGGTCC3110.2526544970063285No Hit
CTCACTTAGTTACAGTTTTATGGATAATTGGGATATTCTTTGGTATAGTT3100.25184210312527927No Hit
CGCCAATCTATATTACTCACAATATCCTAAAAACGATCAGCCGGCAGGTT3040.24696773983898354No Hit
CCGAGTTCAGTGCGACCGTACAGCTCTGGAACCCAAAGGTTCGTTTTTTT3040.24696773983898354No Hit
CGGACAACTGATGGACCCACGTTGCGAGTCCAGTAAATCAGGACGGGGAT3020.24534295207688495No Hit
CCAGAATTCAAGACGTTAACAGTTCTTGGCGCAAATAGCGCTGAATCGCT2980.2420933765526878No Hit
CGGGGAACAGTCGCATGACGAGGCAGTTTCCAGAAACGTGTATCACATCT2880.23396943774219495No Hit
GGCAGTTTCCAGAAACGTGTATCACATCTAGGCATGGAATCTTATGCCAG2880.23396943774219495No Hit
CCCTGACCCCGGGGAACAGTCGCATGACGAGGCAGTTTCCAGAAACGTGT2810.2282826805748499No Hit
GTCGGTTTAGCTGAGATTGCACCGTTGTTATAATCGACCCGGGCTGCGAC2700.21934634788330776No Hit
CCGGGGAACAGTCGCATGACGAGGCAGTTTCCAGAAACGTGTATCACATC2700.21934634788330776No Hit
GTTTTTCTGAGACCTATCCGAGTTCAGTGCGACCGTACAGCTCTGGAACC2700.21934634788330776No Hit
CGCATGACGAGGCAGTTTCCAGAAACGTGTATCACATCTAGGCATGGAAT2670.21690916624015985No Hit
GTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGGGA2670.21690916624015985No Hit
GGGGATAATTGCGCGGTTTTGACTGCGGAAGGAAGCCAAGGCCCACCTAA2670.21690916624015985No Hit
CCTCACTTAGTTACAGTTTTATGGATAATTGGGATATTCTTTGGTATAGT2660.2160967723591106No Hit
CTGGGGTTATGAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGAT2640.214471984597012No Hit
CTGCGATAGGCTAGTTCATAAACGAGGGGCGATGCCCGGGCTCAGAATCC2520.20472325802442054No Hit
GTCGGGGGCACCACACCACGCTCGCTTGGCAGTAACGCGAGCAGGGGGCG2510.20391086414337126No Hit
GGGGAACAGTCGCATGACGAGGCAGTTTCCAGAAACGTGTATCACATCTA2510.20391086414337126No Hit
CTATATTTCGTTTTTCTGAGACCTATCCGAGTTCAGTGCGACCGTACAGC2510.20391086414337126No Hit
CTTAGTTACAGTTTTATGGATAATTGGGATATTCTTTGGTATAGTTGGGA2450.19903650085707553No Hit
GGATAATTGCGCGGTTTTGACTGCGGAAGGAAGCCAAGGCCCACCTAAAG2440.19822410697602627No Hit
GTCGAGTTGTACGGCCGTTCAGCCACGAGTCACGGGGTCTAACGCCTACC2400.1949745314518291No Hit
CTTGGTATACGGACAACTGATGGACCCACGTTGCGAGTCCAGTAAATCAG2340.19010016816553338No Hit
GGCAGGTTCATTTTAAACGCGGTGACTAGGATGCTCATTTGAATGTCCCC2320.1884753804034348No Hit
GGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGAGATGT2310.1876629865223855No Hit
GCCCGTTTCAGGTGGTCCTCAGCGTACGGCGGGACCTCTGAGAATTGGGA2250.18278862323608977No Hit
ATTTTTAGTTATAATGATGCCTTATGTGATAGATGCCTCTTTAAAATATC2250.18278862323608977No Hit
CTTTACAGCAAATACCAGCGGCCCTGACCCCGGGGAACAGTCGCATGACG2230.1811638354739912No Hit
CCCCTCTTTACAGCAAATACCAGCGGCCCTGACCCCGGGGAACAGTCGCA2230.1811638354739912No Hit
TGCCATTCGGACCTACCGTAAGCCTATATTTCGTTTTTCTGAGACCTATC2220.18035144159294192No Hit
CCTCTTTACAGCAAATACCAGCGGCCCTGACCCCGGGGAACAGTCGCATG2220.18035144159294192No Hit
CTGACCCCGGGGAACAGTCGCATGACGAGGCAGTTTCCAGAAACGTGTAT2220.18035144159294192No Hit
TTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTA2220.18035144159294192No Hit
GGGATATTCTTTGGTATAGTTGGGATTTTAATATCATTAATAGCATGATG2220.18035144159294192No Hit
GGATATTCTTTGGTATAGTTGGGATTTTAATATCATTAATAGCATGATGG2190.17791425994979404No Hit
GTCTGCGATAGGCTAGTTCATAAACGAGGGGCGATGCCCGGGCTCAGAAT2170.17628947218769547No Hit
GTCGTCATTGCGCCAGCTCGTCAGCGCTGGTCGTCTACCAGCTCGATAAT2170.17628947218769547No Hit
CTCGGCAATAGGATTGTAGCCCAGGATGAGTCCCTCAGCGTGACGCAGTA2130.17303989666349834No Hit
CGGCAATAGGATTGTAGCCCAGGATGAGTCCCTCAGCGTGACGCAGTAAA2130.17303989666349834No Hit
CTCAGCGTGACGCAGTAAACTAGTAGCATCCCGATCTTTTCCCTATCTAA2110.17141510890139974No Hit
GTTGATACCGTCTGCGATAGGCTAGTTCATAAACGAGGGGCGATGCCCGG2100.17060271502035046No Hit
CGCATATAACCACATTCAAATTGACCTCCCTCAGGAAGCTAAGAAATACT2090.16979032113930118No Hit
CCACATTCAAATTGACCTCCCTCAGGAAGCTAAGAAATACTATCTCGGCA2080.1689779272582519No Hit
CGAGGATGTTCCTGTTGATACCGTCTGCGATAGGCTAGTTCATAAACGAG2050.16654074561510404No Hit
CGGCAGGTTCATTTTAAACGCGGTGACTAGGATGCTCATTTGAATGTCCC2030.16491595785300545No Hit
ATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACCGC2030.16491595785300545No Hit
GTGCTAGCCCGTCGAGACTGAAAAGCTATAACCCGCAGACCCGAGCGAAA1990.1616663823288083No Hit
GCAGTTTCCAGAAACGTGTATCACATCTAGGCATGGAATCTTATGCCAGC1990.1616663823288083No Hit
GGCAATTCGTCTCCACACTAGAAGTCGACGAACAACGAAGAGCGACGATG1970.16004159456670972No Hit
CCCACCTAAAGTATGCTGCAAACATATGCAGATCCCCTGAGACAGAGTCG1960.15922920068566043No Hit
ATTCGGACCTACCGTAAGCCTATATTTCGTTTTTCTGAGACCTATCCGAG1940.15760441292356187No Hit
GTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGATGCCTTATGT1890.15354244351831542No Hit
CGCAAGTTGTAGGTCGGGGGCACCACACCACGCTCGCTTGGCAGTAACGC1870.15191765575621685No Hit
CGGCGCTCTTCCTAGTTGGTACAGAACTGAGTGTCATGTGTCCAAAGTTA1820.1478556863509704No Hit
CCGCAGACCCGAGCGAAAGCGGCGGTCCTTACAAGTCCGCTCCTCGGGGA1800.14623089858887184No Hit
CCTGTTGATACCGTCTGCGATAGGCTAGTTCATAAACGAGGGGCGATGCC1790.14541850470782255No Hit
CTCAAAAGGGTGTACACCGCCCTTAGACGTCTTGGTATACGGACAACTGA1780.14460611082677324No Hit
AGACAGAGTCGCTATCGTTATGTCTCCTTCCCGCGGTCAAGGCGAAACCG1770.14379371694572396No Hit
GTCCTTACAAGTCCGCTCCTCGGGGAGCTTGATTGATAATTCTGTATAAG1760.14298132306467468No Hit
GTTGGAACTCTACCAATTGGAGCTTTCTTAGCTGTCTTAGCAGTAGTTTA1750.1421689291836254No Hit
GCGGTTTTGACTGCGGAAGGAAGCCAAGGCCCACCTAAAGTATGCTGCAA1750.1421689291836254No Hit
ATGGAATCTTATGCCAGCTAACGGAACAAGCTTTGTGCCATTCGGACCTA1730.1405441414215268No Hit
CTCTTTACAGCAAATACCAGCGGCCCTGACCCCGGGGAACAGTCGCATGA1730.1405441414215268No Hit
CTTAGCAGTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGATGC1730.1405441414215268No Hit
CCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGGGATGAGCATAAA1720.13973174754047754No Hit
GTTTTTTTCTTGGTACCTATTCCTCCAGGAATTACTGACCATAGTGCTCG1680.13648217201628038No Hit
GGTAAAACGCAAGCACCGGCTGTCGAGTTGTACGGCCGTTCAGCCACGAG1680.13648217201628038No Hit
CGCAGTTAATAATTCTGGCAATTCGTCTCCACACTAGAAGTCGACGAACA1670.13566977813523107No Hit
CCCAGGATGAGTCCCTCAGCGTGACGCAGTAAACTAGTAGCATCCCGATC1670.13566977813523107No Hit
CGCGGTTTTGACTGCGGAAGGAAGCCAAGGCCCACCTAAAGTATGCTGCA1620.13160780872998465No Hit
GTGTGCTAGCCCGTCGAGACTGAAAAGCTATAACCCGCAGACCCGAGCGA1620.13160780872998465No Hit
TTCCGCTTTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACA1620.13160780872998465No Hit
CTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTT1610.13079541484893537No Hit
CTCTGGAACCCAAAGGTTCGTTTTTTTCTTGGTACCTATTCCTCCAGGAA1610.13079541484893537No Hit
CCAGGAGATGTTGGAACTCTACCAATTGGAGCTTTCTTAGCTGTCTTAGC1600.12998302096788608No Hit
CACTTAGTTACAGTTTTATGGATAATTGGGATATTCTTTGGTATAGTTGG1580.12835823320578751No Hit
ATCTAATTCACCTCCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAA1560.12673344544368892No Hit
CAGCAAAGGTTTTTCCAGGAGATGTTGGAACTCTACCAATTGGAGCTTTC1560.12673344544368892No Hit
TGGCAATTCGTCTCCACACTAGAAGTCGACGAACAACGAAGAGCGACGAT1550.12592105156263964No Hit
CGAACAACGAAGAGCGACGATGCCCGTTTCAGGTGGTCCTCAGCGTACGG1550.12592105156263964No Hit
TCCGCTTTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAA1540.12510865768159035No Hit
GCCAGCTAACGGAACAAGCTTTGTGCCATTCGGACCTACCGTAAGCCTAT1540.12510865768159035No Hit
CTCGTTCTTTGGTCGCAATCCTGCGTAATCAACGCCGCAACTTTACGTCG1530.12429626380054104No Hit
TGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAACCAATGAGAG1500.12185908215739318No Hit
GGGTAAAACGCAAGCACCGGCTGTCGAGTTGTACGGCCGTTCAGCCACGA1490.1210466882763439No Hit
CGCAGACCCGAGCGAAAGCGGCGGTCCTTACAAGTCCGCTCCTCGGGGAG1480.12023429439529461No Hit
ACCAGATGTGTGCTAGCCCGTCGAGACTGAAAAGCTATAACCCGCAGACC1470.11942190051424534No Hit
CCGATCTTTTCCCTATCTAATTCACCTCCTATTAGGAGCCGATCGTGCTT1470.11942190051424534No Hit
GTTGATTTCATGAATGCGATTTCTGATATGGCGGCGGTCGGTTTCCAGTT1440.11698471887109747No Hit
CAGCGGCCCTGACCCCGGGGAACAGTCGCATGACGAGGCAGTTTCCAGAA1430.11617232499004818No Hit
CCCAGCGGCTAGCATGTGTAGTGCGCCATATCATATCCAAGATAGTGTAG1430.11617232499004818No Hit
GTACACCGCCCTTAGACGTCTTGGTATACGGACAACTGATGGACCCACGT1430.11617232499004818No Hit
CTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGGGATGAGCATAAAC1420.11535993110899888No Hit
CGAGACTGAAAAGCTATAACCCGCAGACCCGAGCGAAAGCGGCGGTCCTT1420.11535993110899888No Hit
CGATAATTGGCGCAAGTTGTAGGTCGGGGGCACCACACCACGCTCGCTTG1410.11454753722794961No Hit
CTGAGACCTATCCGAGTTCAGTGCGACCGTACAGCTCTGGAACCCAAAGG1400.11373514334690031No Hit
ATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGG1390.11292274946585101No Hit
CGCAAGCACCGGCTGTCGAGTTGTACGGCCGTTCAGCCACGAGTCACGGG1390.11292274946585101No Hit
GGATAATTGGGATATTCTTTGGTATAGTTGGGATTTTAATATCATTAATA1380.11211035558480174No Hit
CCCTAACTGCGCAGTTAATAATTCTGGCAATTCGTCTCCACACTAGAAGT1370.11129796170375245No Hit
GAGCTTTCTTAGCTGTCTTAGCAGTAGTTTATAAGGAATATATCCCATTT1370.11129796170375245No Hit
CCTAAAGTATGCTGCAAACATATGCAGATCCCCTGAGACAGAGTCGCTAT1360.11048556782270316No Hit
GTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAACCAATGAGA1360.11048556782270316No Hit
CAGCTCTGGAACCCAAAGGTTCGTTTTTTTCTTGGTACCTATTCCTCCAG1350.10967317394165388No Hit
GCGGGACCTCTGAGAATTGGGATACAGGACCCAAAAGGCTGAAAGGGGGC1350.10967317394165388No Hit
GTTTATAAGGAATATATCCCATTTTTAGTTATAATGATGCCTTATGTGAT1340.10886078006060458No Hit
GGGAAGCTATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATT1340.10886078006060458No Hit
TGAGAATTGGGATACAGGACCCAAAAGGCTGAAAGGGGGCAGTGAAGTCG1340.10886078006060458No Hit
CCCGCTTTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAA1330.1080483861795553No Hit
CCAGCGGCTAGCATGTGTAGTGCGCCATATCATATCCAAGATAGTGTAGG1330.1080483861795553No Hit
CCTAACTGCGCAGTTAATAATTCTGGCAATTCGTCTCCACACTAGAAGTC1320.107235992298506No Hit
CTAGTTCATAAACGAGGGGCGATGCCCGGGCTCAGAATCCAAGGAGCAAA1320.107235992298506No Hit
GCGGCCCTGACCCCGGGGAACAGTCGCATGACGAGGCAGTTTCCAGAAAC1320.107235992298506No Hit
CCCGTCGAGACTGAAAAGCTATAACCCGCAGACCCGAGCGAAAGCGGCGG1270.10317402289325957No Hit
CTACCGTAAGCCTATATTTCGTTTTTCTGAGACCTATCCGAGTTCAGTGC1250.101549235131161No Hit
CGGGGATAATTGCGCGGTTTTGACTGCGGAAGGAAGCCAAGGCCCACCTA1240.1007368412501117No Hit
CCGGCAGGTTCATTTTAAACGCGGTGACTAGGATGCTCATTTGAATGTCC1240.1007368412501117No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)