FastQCFastQC Report
Wed 10 Aug 2022
BORED_3_R2.fq

Summary

[OK]Basic Statistics

MeasureValue
FilenameBORED_3_R2.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences123093
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCT11630.9448140836603218No Hit
CCTAACTTAATGGACGGGAGGTATCCCAATAGGAGGTTTCCTCCTATGGT5960.4841867531053756No Hit
CTTAGATATTTTAAAGAGGCATCTATCACATAAGGCATCATTATAACTAA5920.48093717758117843No Hit
CTCGTAACCAAACATGCACAGCGGTCAAACAGTATGTCCCAAGGGGACTT5220.42406960590772835No Hit
CTTCCATTTCCGCCCAAGCTGCTCACAGTATACGGGCGTCGGCATCCAGA5030.4086341221677918No Hit
CTAACTTAATGGACGGGAGGTATCCCAATAGGAGGTTTCCTCCTATGGTT4550.369639215877426No Hit
CCCTAACTTAATGGACGGGAGGTATCCCAATAGGAGGTTTCCTCCTATGG4470.3631400648290317No Hit
CCCAATTATCCATAAAACTGTAACTAAGTGAGGCTCTCTCATTGGTTTAT4460.3623276709479824No Hit
CTCTCATTGGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCA4450.36151527706693315No Hit
CGAATAGCTCGTAACCAAACATGCACAGCGGTCAAACAGTATGTCCCAAG4030.32739473406286307No Hit
CGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGGCTAGA3890.316021219728173No Hit
CTTCACTGCCCCCTTTCAGCCTTTTGGGTCCTGTATCCCAATTCTCAGAG3780.30708488703663084No Hit
CTGAACTCGGATAGGTCTCAGAAAAACGAAATATAGGCTTACGGTAGGTC3620.29408658493984224No Hit
CTGAGGACCACCTGAAACGGGCATCGTCGCTCTTCGTTGTTCGTCGACTT3550.28839982777249723No Hit
CATTAATTAGGGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAAT3520.28596264612934935No Hit
CCTGAAACGGGCATCGTCGCTCTTCGTTGTTCGTCGACTTCTAGTGTGGA3510.28515025224830004No Hit
GGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGGAGGTTTC3400.2762139195567579No Hit
AAAAAAACGAACCTTTGGGTTCCAGAGCTGTACGGTCGCACTGAACTCGG3400.2762139195567579No Hit
GCTGCTCACAGTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGG3290.26727758686521574No Hit
CAATTATCCATAAAACTGTAACTAAGTGAGGCTCTCTCATTGGTTTATAC3110.2526544970063285No Hit
GTCGCACTGAACTCGGATAGGTCTCAGAAAAACGAAATATAGGCTTACGG3100.25184210312527927No Hit
GCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGGAGGTTTCC3090.25102970924422996No Hit
GGGATAGATAGCCACCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTT3070.2494049214821314No Hit
GACGAATTGCCAGAATTATTAACTGCGCAGTTAGGGCAGCGTCTGAGGAA3030.24615534595793426No Hit
CGTGGTTTTACTAGGCTAGACTAGCGTACGAGCACTATGGTCAGTAATTC3020.24534295207688495No Hit
GGCCGATTGTCGCAGCCCGGGTCGATTATAACAACGGTGCAATCTCAGCT2910.2364066193853428No Hit
CTGTAACTAAGTGAGGCTCTCTCATTGGTTTATACTTCAATATAAGCCTT2870.23315704386114564No Hit
CTCTCTCATTGGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGC2850.23153225609904704No Hit
CTAGCGTACGAGCACTATGGTCAGTAATTCCTGGAGGAATAGGTACCAAG2750.22340831728855418No Hit
AAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTCTCTCA2740.2225959234075049No Hit
CTTATACAGAATTATCAATCAAGCTCCCCGAGGAGCGGACTTGTAAGGAC2710.22015874176435704No Hit
CCCAATTCTCAGAGGTCCCGCCGTACGCTGAGGACCACCTGAAACGGGCA2640.214471984597012No Hit
GAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTCTCTCATTG2570.208785227429667No Hit
GTTTATTGGAAGCATCAACCTGCGCCGTCTTGTTAACTTGTCATATCGCG2540.20634804578651914No Hit
TATAGATTGGCGCTAGTAGATCACAGAACAACCGCCGCATACGGCCGATT2530.20553565190546985No Hit
CGCGAATTGGGGACATTCAAATGAGCATCCTAGTCACCGCGTTTAAAATG2530.20553565190546985No Hit
ATTACTTCCATTTCCGCCCAAGCTGCTCACAGTATACGGGCGTCGGCATC2490.20228607638127272No Hit
CCTTGACCGCGGGAAGGAGACATAACGATAGCGACTCTGTCTCAGGGGAT2460.1998488947381248No Hit
GTGAGGCTCTCTCATTGGTTTATACTTCAATATAAGCCTTGGTAGGGATA2460.1998488947381248No Hit
GTCAAAACCGCGCAATTATCCCCGTCCTGATTTACTGGACTCGCAACGTG2400.1949745314518291No Hit
ATTAGGGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGGAG2390.19416213757077982No Hit
CTTGGCTTCCTTCCGCAGTCAAAACCGCGCAATTATCCCCGTCCTGATTT2360.19172495592763195No Hit
CTGAAACGGGCATCGTCGCTCTTCGTTGTTCGTCGACTTCTAGTGTGGAG2350.19091256204658266No Hit
CCTCGATCCATCGCGTATAGGGACGCCCCCTGCTCGCGTTACTGCCAAGC2350.19091256204658266No Hit
GTTGGTTTATGCTCATCCCTACTCATAACCCCAGCACTTAGATATTTTAA2340.19010016816553338No Hit
GTTAGGGCAGCGTCTGAGGAAGTTTGCTGCGGTTTCGCCTTGACCGCGGG2330.1892877742844841No Hit
CACGTTTCTGGAAACTGCCTCGTCATGCGACTGTTCCCCGGGGTCAGGGC2250.18278862323608977No Hit
GTTTTACTAGGCTAGACTAGCGTACGAGCACTATGGTCAGTAATTCCTGG2210.17953904771189264No Hit
TCCATTTCCGCCCAAGCTGCTCACAGTATACGGGCGTCGGCATCCAGACC2190.17791425994979404No Hit
CTCATCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTA2180.17710186606874478No Hit
CGCTGGTATTTGCTGTAAAGAGGGGCGTTGAGTCCGTCCGACTTCACTGC2150.1746646844255969No Hit
ATTAATTAGGGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATA2120.17222750278244905No Hit
CTCGGATAGGTCTCAGAAAAACGAAATATAGGCTTACGGTAGGTCCGAAT2120.17222750278244905No Hit
CGAATTGCCAGAATTATTAACTGCGCAGTTAGGGCAGCGTCTGAGGAAGT2120.17222750278244905No Hit
CACAAAGCTTGTTCCGTTAGCTGGCATAAGATTCCATGCCTAGATGTGAT2100.17060271502035046No Hit
CTTTTGGGTCCTGTATCCCAATTCTCAGAGGTCCCGCCGTACGCTGAGGA2070.1681655333772026No Hit
GCTGTACGGTCGCACTGAACTCGGATAGGTCTCAGAAAAACGAAATATAG2050.16654074561510404No Hit
TTATAAACTACTGCTAAGACAGCTAAGAAAGCTCCAATTGGTAGAGTTCC2040.16572835173405473No Hit
CAAGCTGCTCACAGTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCG2040.16572835173405473No Hit
CTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGGAGGTTTCCT2030.16491595785300545No Hit
CTTGACCGCGGGAAGGAGACATAACGATAGCGACTCTGTCTCAGGGGATC2020.16410356397195616No Hit
GAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGGAGGTTTCCTCC1990.1616663823288083No Hit
CCAAGCTGCTCACAGTATACGGGCGTCGGCATCCAGACCGTCGGCTGATC1980.160853988447759No Hit
CGTCGCTCTTCGTTGTTCGTCGACTTCTAGTGTGGAGACGAATTGCCAGA1980.160853988447759No Hit
AGAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTCTCTCATT1980.160853988447759No Hit
GGGAGGTATCCCAATAGGAGGTTTCCTCCTATGGTTTTCAAAACAATCAC1970.16004159456670972No Hit
GGATAGATAGCCACCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTG1930.15679201904251258No Hit
CTCAGCTAAACCGACGCAGTTTTGCTCCTTGGATTCTGAGCCCGGGCATC1910.15516723128041401No Hit
AGAAAAACGAAATATAGGCTTACGGTAGGTCCGAATGGCACAAAGCTTGT1900.1543548373993647No Hit
GGGGCGTTGAGTCCGTCCGACTTCACTGCCCCCTTTCAGCCTTTTGGGTC1850.15029286799411828No Hit
GCGGGTTATAGCTTTTCAGTCTCGACGGGCTAGCACACATCTGGTTGACT1790.14541850470782255No Hit
CTAGCCTATCGCAGACGGTATCAACAGGAACATCCTCGTGTTAGATATTG1780.14460611082677324No Hit
CATAACGATAGCGACTCTGTCTCAGGGGATCTGCATATGTTTGCAGCATA1760.14298132306467468No Hit
TATAACTAAAAATGGGATATATTCCTTATAAACTACTGCTAAGACAGCTA1750.1421689291836254No Hit
CAAACATGCACAGCGGTCAAACAGTATGTCCCAAGGGGACTTAAGCGCGG1750.1421689291836254No Hit
CAGGAACATCCTCGTGTTAGATATTGAGGCTGCTTCGTGTCGGCACGAAG1730.1405441414215268No Hit
GTTAACTTGTCATATCGCGCACGTAGTAGCCTAGAGCGCCAGGGGCGGAA1730.1405441414215268No Hit
GTCAAACAGTATGTCCCAAGGGGACTTAAGCGCGGTGGCCTCCCCTATCC1720.13973174754047754No Hit
GCCCAAGCTGCTCACAGTATACGGGCGTCGGCATCCAGACCGTCGGCTGA1720.13973174754047754No Hit
TTATAACTAAAAATGGGATATATTCCTTATAAACTACTGCTAAGACAGCT1720.13973174754047754No Hit
CTGGTATTTGCTGTAAAGAGGGGCGTTGAGTCCGTCCGACTTCACTGCCC1700.13810695977837895No Hit
CCAGCACTTAGATATTTTAAAGAGGCATCTATCACATAAGGCATCATTAT1690.13729456589732966No Hit
CTATCACATAAGGCATCATTATAACTAAAAATGGGATATATTCCTTATAA1680.13648217201628038No Hit
CTTCTAGTGTGGAGACGAATTGCCAGAATTATTAACTGCGCAGTTAGGGC1680.13648217201628038No Hit
GCCACCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTAT1670.13566977813523107No Hit
GGGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGGAGGTTT1670.13566977813523107No Hit
CACAGAACAACCGCCGCATACGGCCGATTGTCGCAGCCCGGGTCGATTAT1640.13323259649208322No Hit
CCCGGATCGATGACGCGAATTGGGGACATTCAAATGAGCATCCTAGTCAC1640.13323259649208322No Hit
AGCTGCTCACAGTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTG1620.13160780872998465No Hit
AGAACAACCGCCGCATACGGCCGATTGTCGCAGCCCGGGTCGATTATAAC1610.13079541484893537No Hit
CAGCGGTCAAACAGTATGTCCCAAGGGGACTTAAGCGCGGTGGCCTCCCC1610.13079541484893537No Hit
ATTCCTTATAAACTACTGCTAAGACAGCTAAGAAAGCTCCAATTGGTAGA1590.12917062708683677No Hit
CTCGGGTCTGCGGGTTATAGCTTTTCAGTCTCGACGGGCTAGCACACATC1570.1275458393247382No Hit
GGCTAGACTAGCGTACGAGCACTATGGTCAGTAATTCCTGGAGGAATAGG1570.1275458393247382No Hit
GCATATGTTTGCAGCATACTTTAGGTGGGCCTTGGCTTCCTTCCGCAGTC1560.12673344544368892No Hit
GAACAACCGCCGCATACGGCCGATTGTCGCAGCCCGGGTCGATTATAACA1560.12673344544368892No Hit
GAAAAACGAAATATAGGCTTACGGTAGGTCCGAATGGCACAAAGCTTGTT1560.12673344544368892No Hit
CTCAGAAAAACGAAATATAGGCTTACGGTAGGTCCGAATGGCACAAAGCT1560.12673344544368892No Hit
GATAGATAGCCACCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGT1550.12592105156263964No Hit
GTCCGAATGGCACAAAGCTTGTTCCGTTAGCTGGCATAAGATTCCATGCC1530.12429626380054104No Hit
CACCCTTTTGAGCAATGATTGCACAACCTGCGATCACCTTATACAGAATT1530.12429626380054104No Hit
ATCACATAAGGCATCATTATAACTAAAAATGGGATATATTCCTTATAAAC1520.12348386991949177No Hit
CCGGGGTCAGGGCCGCTGGTATTTGCTGTAAAGAGGGGCGTTGAGTCCGT1520.12348386991949177No Hit
CTAGGCTAGACTAGCGTACGAGCACTATGGTCAGTAATTCCTGGAGGAAT1510.12267147603844247No Hit
TATAAACTACTGCTAAGACAGCTAAGAAAGCTCCAATTGGTAGAGTTCCA1500.12185908215739318No Hit
CTCAGAGGTCCCGCCGTACGCTGAGGACCACCTGAAACGGGCATCGTCGC1500.12185908215739318No Hit
TGGGAGTATTTGCACTTGTGGTAACGGTATTTGCATTATTGATGGTTTTT1480.12023429439529461No Hit
CGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGGCTAGACTAGCGT1480.12023429439529461No Hit
GAAAGATCATGTTTATTGGAAGCATCAACCTGCGCCGTCTTGTTAACTTG1470.11942190051424534No Hit
GTCGACTTCTAGTGTGGAGACGAATTGCCAGAATTATTAACTGCGCAGTT1460.11860950663319604No Hit
GCCGCTGGTATTTGCTGTAAAGAGGGGCGTTGAGTCCGTCCGACTTCACT1440.11698471887109747No Hit
CTGATCGTGGTTTTACTAGGCTAGACTAGCGTACGAGCACTATGGTCAGT1410.11454753722794961No Hit
TAGATAGCCACCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTG1400.11373514334690031No Hit
CCCAACTATACCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGT1400.11373514334690031No Hit
CGTAACCAAACATGCACAGCGGTCAAACAGTATGTCCCAAGGGGACTTAA1390.11292274946585101No Hit
CTCATTGGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACC1370.11129796170375245No Hit
CCTGGAGGAATAGGTACCAAGAAAAAAACGAACCTTTGGGTTCCAGAGCT1370.11129796170375245No Hit
TTCCATTTCCGCCCAAGCTGCTCACAGTATACGGGCGTCGGCATCCAGAC1360.11048556782270316No Hit
CTTGGATCAGTGGGAAGTGCTCACGCGCGGAGCCCACTGGGCGAACAGCA1340.10886078006060458No Hit
CATCATTATAACTAAAAATGGGATATATTCCTTATAAACTACTGCTAAGA1330.1080483861795553No Hit
GCTTACGGTAGGTCCGAATGGCACAAAGCTTGTTCCGTTAGCTGGCATAA1320.107235992298506No Hit
CAGCACTTAGATATTTTAAAGAGGCATCTATCACATAAGGCATCATTATA1320.107235992298506No Hit
GGAACATCCTCGTGTTAGATATTGAGGCTGCTTCGTGTCGGCACGAAGTG1290.10479881065535814No Hit
CTTACGGTAGGTCCGAATGGCACAAAGCTTGTTCCGTTAGCTGGCATAAG1290.10479881065535814No Hit
ACTTAATGGACGGGAGGTATCCCAATAGGAGGTTTCCTCCTATGGTTTTC1280.10398641677430885No Hit
TGAGGCTCTCTCATTGGTTTATACTTCAATATAAGCCTTGGTAGGGATAG1270.10317402289325957No Hit
CTTAATGGACGGGAGGTATCCCAATAGGAGGTTTCCTCCTATGGTTTTCA1270.10317402289325957No Hit
CCTGTATCCCAATTCTCAGAGGTCCCGCCGTACGCTGAGGACCACCTGAA1270.10317402289325957No Hit
TAAAACTGTAACTAAGTGAGGCTCTCTCATTGGTTTATACTTCAATATAA1260.10236162901221027No Hit
GCTCGAGTTGCCCCTTTGCCCGATCCCGGAAGATACGCTCTAAGCTCGGC1260.10236162901221027No Hit
CTTTAGGTGGGCCTTGGCTTCCTTCCGCAGTCAAAACCGCGCAATTATCC1260.10236162901221027No Hit
GGCCGCTGGTATTTGCTGTAAAGAGGGGCGTTGAGTCCGTCCGACTTCAC1260.10236162901221027No Hit
CTGTATCCCAATTCTCAGAGGTCCCGCCGTACGCTGAGGACCACCTGAAA1250.101549235131161No Hit
CCCAATAGGAGGTTTCCTCCTATGGTTTTCAAAACAATCACCATCATGCT1240.1007368412501117No Hit
CGAACCTTTGGGTTCCAGAGCTGTACGGTCGCACTGAACTCGGATAGGTC1240.1007368412501117No Hit
GCTCATCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCT1240.1007368412501117No Hit
GCCGTACGCTGAGGACCACCTGAAACGGGCATCGTCGCTCTTCGTTGTTC1240.1007368412501117No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)