FastQCFastQC Report
Wed 10 Aug 2022
EXCITED_1_R1.fq

Summary

[OK]Basic Statistics

MeasureValue
FilenameEXCITED_1_R1.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences237018
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCAGAATTCAAGACGTTAACAGTTCTTGGCGCAAATAGCGCTGAATCGCT21430.9041507396062746No Hit
CTTTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATAT11720.4944772127011451No Hit
GTTGATTTCATGAATGCGATTTCTGATATGGCGGCGGTCGGTTTCCAGTT11510.48561712612544194No Hit
CTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGT11050.4662093174358065No Hit
GCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGA10600.4472234176307285No Hit
GCCTTCTCTCGTTCTCGCCCGCTTTGCAAAAATATCTAATATCAATTGCG9900.4176897957117181No Hit
GTCGGAAGATCCTGAATAAATCCTACTGTATCTGAAAGAAGAACACTGTA8580.3619978229501557No Hit
GGAAGCTATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTG7780.3282451121855724No Hit
CTTCTCTCGTTCTCGCCCGCTTTGCAAAAATATCTAATATCAATTGCGTG7740.32655747664734325No Hit
CGCTTTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAAT7640.3223383878017704No Hit
CTCAATTTGAAGTTTGCCTTCTCTCGTTCTCGCCCGCTTTGCAAAAATAT7570.3193850256098693No Hit
CCGCTTTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAA7100.2995553080356766No Hit
CCGGGATCGGGCAAAGGGGCAACTCGAGCTAATCTCCCCAGCGGCTAGCA7040.29702385472833287No Hit
GGTCAATCATCTTCACTTCAATTGCTGTTGCCAATGACTTCAGCTGACTT6780.2860542237298433No Hit
GTAAAACGCAAGCACCGGCTGTCGAGTTGTACGGCCGTTCAGCCACGAGT6670.2814132259997131No Hit
CTTCACTTCAATTGCTGTTGCCAATGACTTCAGCTGACTTGGCGACAGTT6630.27972559046148393No Hit
CAGAATTCAAGACGTTAACAGTTCTTGGCGCAAATAGCGCTGAATCGCTT6530.275506501615911No Hit
GTCAGCACTCGTCAGGCGGTTGAACCATGTTGATTTCCCTGCGTTTGTAT6330.2670683239247652No Hit
GTTATGAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAA6240.2632711439637496No Hit
CGGCTGTCGAGTTGTACGGCCGTTCAGCCACGAGTCACGGGGTCTAACGC6230.2628492350791923No Hit
ATCGGGTCCAGCGTGGCAAACAGGAGGTCTTCTTCATAGCTGTCAGCACT5930.25019196854247355No Hit
CAATGACTTCAGCTGACTTGGCGACAGTTCATCATTAAAGATGAGGAGAT5900.2489262418888017No Hit
GCCTCACTTAGTTACAGTTTTATGGATAATTGGGATATTCTTTGGTATAG5840.24639478858145794No Hit
GTCGGTTTAGCTGAGATTGCACCGTTGTTATAATCGACCCGGGCTGCGAC5770.24344142638955693No Hit
CCCATTTTTAGTTATAATGATGCCTTATGTGATAGATGCCTCTTTAAAAT5590.23584706646752568No Hit
GGAAGATCCTGAATAAATCCTACTGTATCTGAAAGAAGAACACTGTAGCC5580.23542515758296836No Hit
GTCCAGCGTGGCAAACAGGAGGTCTTCTTCATAGCTGTCAGCACTCGTCA5560.23458133981385382No Hit
CCGGGACCTCTTGCCCCAATTCCTCCGCCTTGCCGGGAAAGGTTGATCCC5550.23415943092929653No Hit
GTCGGGGGCACCACACCACGCTCGCTTGGCAGTAACGCGAGCAGGGGGCG5510.23247179539106735No Hit
CTCAAATTTCGCACTGACCATAATGTGATCCCTTCCGGCGGTCGGTATAA5460.2303622509682809No Hit
CTGTGATCTACTAGCGCCAATCTATATTACTCACAATATCCTAAAAACGA5320.22445552658447881No Hit
CTGAGACAGAGTCGCTATCGTTATGTCTCCTTCCCGCGGTCAAGGCGAAA5260.2219240732771351No Hit
AGGAGATGTTGGAACTCTACCAATTGGAGCTTTCTTAGCTGTCTTAGCAG5250.22150216439257778No Hit
CCGCATAATCCTCATTTGAAGAATCAATTAAATGCAGAATTAAATCCGCT5220.2202364377389059No Hit
GGCAGGTTCATTTTAAACGCGGTGACTAGGATGCTCATTTGAATGTCCCC5090.2147516222396611No Hit
CTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGGGATGAGCATAAACCA5040.2126420778168747No Hit
CTGCGTTTGTATAGCCGACAAGCGCAATTTGAAGCACACCGTTTTTCTTT5020.2117982600477601No Hit
GTGCGGTCAATCATCTTCACTTCAATTGCTGTTGCCAATGACTTCAGCTG4920.20757917120218716No Hit
GTCGGTTTCCAGTTTCGTTTCCCCGGGACCTCTTGCCCCAATTCCTCCGC4900.2067353534330726No Hit
GTCGGTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAG4780.20167244681838511No Hit
CTCGTTCTCGCCCGCTTTGCAAAAATATCTAATATCAATTGCGTGCGGTC4670.1970314490882549No Hit
CTCAAGCGTTGAGCGGAATGCAGCAATCAATGTCGTCGGAAGATCCTGAA4630.19534381355002572No Hit
GTCGTCATTGCGCCAGCTCGTCAGCGCTGGTCGTCTACCAGCTCGATAAT4590.19365617801179658No Hit
CTCTCGTTCTCGCCCGCTTTGCAAAAATATCTAATATCAATTGCGTGCGG4590.19365617801179658No Hit
GTCTTCTTCATAGCTGTCAGCACTCGTCAGGCGGTTGAACCATGTTGATT4580.19323426912723926No Hit
CAGCAATCAATGTCGTCGGAAGATCCTGAATAAATCCTACTGTATCTGAA4550.1919685424735674No Hit
CGCCAATCTATATTACTCACAATATCCTAAAAACGATCAGCCGGCAGGTT4520.19070281581989554No Hit
GGGGTTATGAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGG4490.18943708916622368No Hit
CTCAGACGCTGCCCTAACTGCGCAGTTAATAATTCTGGCAATTCGTCTCC4470.1885932713971091No Hit
GTCTGCGATAGGCTAGTTCATAAACGAGGGGCGATGCCCGGGCTCAGAAT4460.1881713625125518No Hit
GTTGATACCGTCTGCGATAGGCTAGTTCATAAACGAGGGGCGATGCCCGG4370.18437418255153618No Hit
CCCAATTCCTCCGCCTTGCCGGGAAAGGTTGATCCCTTGTCCCGTCAGAC4350.18353036478242157No Hit
GGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATCGGGATATCATC4350.18353036478242157No Hit
GTCAGCATCGGGATATCATCTGCTTCAAGCTCCTCAAGCAGCCGAAGCAC4330.182686547013307No Hit
GTCAGACGCGGCAGTGCATATTGCAGCTGAGCCAGCTCAATTTGAAGTTT4320.1822646381287497No Hit
GCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGGGATGAGCATAA4290.18099891147507785No Hit
GTCAATCATCTTCACTTCAATTGCTGTTGCCAATGACTTCAGCTGACTTG4120.1738264604376039No Hit
GCCATTCGGACCTACCGTAAGCCTATATTTCGTTTTTCTGAGACCTATCC4080.17213882489937474No Hit
GTGCATATTGCAGCTGAGCCAGCTCAATTTGAAGTTTGCCTTCTCTCGTT4060.17129500713026014No Hit
GGACAACTGATGGACCCACGTTGCGAGTCCAGTAAATCAGGACGGGGATA4050.17087309824570285No Hit
CCACATTCAAATTGACCTCCCTCAGGAAGCTAAGAAATACTATCTCGGCA4030.17002928047658827No Hit
CTGCGATAGGCTAGTTCATAAACGAGGGGCGATGCCCGGGCTCAGAATCC4030.17002928047658827No Hit
GCCAGCTCAATTTGAAGTTTGCCTTCTCTCGTTCTCGCCCGCTTTGCAAA4030.17002928047658827No Hit
CTCACTTAGTTACAGTTTTATGGATAATTGGGATATTCTTTGGTATAGTT4010.1691854627074737No Hit
CTTCTTCATAGCTGTCAGCACTCGTCAGGCGGTTGAACCATGTTGATTTC3990.16834164493835913No Hit
ATCACATCTAGGCATGGAATCTTATGCCAGCTAACGGAACAAGCTTTGTG3980.1679197360538018No Hit
ATCTAATTCACCTCCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAA3930.16581019163101535No Hit
GGGATAATTGCGCGGTTTTGACTGCGGAAGGAAGCCAAGGCCCACCTAAA3920.1653882827464581No Hit
GTTTTTTCATGTCCCGCATAATCCTCATTTGAAGAATCAATTAAATGCAG3900.16454446497734349No Hit
GCCGTCAGCATCGGGATATCATCTGCTTCAAGCTCCTCAAGCAGCCGAAG3890.1641225560927862No Hit
CTCGGCAATAGGATTGTAGCCCAGGATGAGTCCCTCAGCGTGACGCAGTA3840.16201301166999976No Hit
GTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGGGA3830.16159110278544245No Hit
CAATCATCTTCACTTCAATTGCTGTTGCCAATGACTTCAGCTGACTTGGC3810.16074728501632787No Hit
ATTTTTAGTTATAATGATGCCTTATGTGATAGATGCCTCTTTAAAATATC3780.15948155836265598No Hit
CGGCAGGTTCATTTTAAACGCGGTGACTAGGATGCTCATTTGAATGTCCC3780.15948155836265598No Hit
GTCCCGCATAATCCTCATTTGAAGAATCAATTAAATGCAGAATTAAATCC3740.15779392282442684No Hit
GTCTCCTTCCCGCGGTCAAGGCGAAACCGCAGCAAACTTCCTCAGACGCT3740.15779392282442684No Hit
GTGGAAAGCTGTGTGTTGATTTCATGAATGCGATTTCTGATATGGCGGCG3710.15652819617075497No Hit
GTTGATTTCCCTGCGTTTGTATAGCCGACAAGCGCAATTTGAAGCACACC3690.1556843784016404No Hit
CGGCAATAGGATTGTAGCCCAGGATGAGTCCCTCAGCGTGACGCAGTAAA3620.15273101620973936No Hit
GGCGAATGACAGTGGAAAGCTGTGTGTTGATTTCATGAATGCGATTTCTG3550.1497776540178383No Hit
GGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGAGATGT3500.14766810959505183No Hit
GGCAGTTTCCAGAAACGTGTATCACATCTAGGCATGGAATCTTATGCCAG3480.14682429182593726No Hit
GTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGATGCCTTATGT3460.14598047405682268No Hit
GTTTATTGTAAGCCGTCAGCATCGGGATATCATCTGCTTCAAGCTCCTCA3460.14598047405682268No Hit
CGCATATAACCACATTCAAATTGACCTCCCTCAGGAAGCTAAGAAATACT3430.14471474740315082No Hit
GCAGCAATCAATGTCGTCGGAAGATCCTGAATAAATCCTACTGTATCTGA3430.14471474740315082No Hit
CCCGCATAATCCTCATTTGAAGAATCAATTAAATGCAGAATTAAATCCGC3380.14260520298036436No Hit
GTGTACACCGCCCTTAGACGTCTTGGTATACGGACAACTGATGGACCCAC3370.14218329409580707No Hit
CTCAGCGTGACGCAGTAAACTAGTAGCATCCCGATCTTTTCCCTATCTAA3260.13754229636567686No Hit
CGCTTCTTTAAAGGCTGCAGCGTCGTCCTCAAATTTCGCACTGACCATAA3230.136276569712005No Hit
CTGGGGTTATGAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGAT3220.1358546608274477No Hit
GGGGATAATTGCGCGGTTTTGACTGCGGAAGGAAGCCAAGGCCCACCTAA3220.1358546608274477No Hit
CCTCACTTAGTTACAGTTTTATGGATAATTGGGATATTCTTTGGTATAGT3190.13458893417377585No Hit
CCTGTTGATACCGTCTGCGATAGGCTAGTTCATAAACGAGGGGCGATGCC3190.13458893417377585No Hit
CGCATAATCCTCATTTGAAGAATCAATTAAATGCAGAATTAAATCCGCTT3180.13416702528921853No Hit
CTTACAAGTCCGCTCCTCGGGGAGCTTGATTGATAATTCTGTATAAGGTG3180.13416702528921853No Hit
CGAGGATGTTCCTGTTGATACCGTCTGCGATAGGCTAGTTCATAAACGAG3120.1316355719818748No Hit
CGATAATTGGCGCAAGTTGTAGGTCGGGGGCACCACACCACGCTCGCTTG3060.12910411867453106No Hit
CCCGTTTCAGGTGGTCCTCAGCGTACGGCGGGACCTCTGAGAATTGGGAT3040.12826030090541649No Hit
CCGATCTTTTCCCTATCTAATTCACCTCCTATTAGGAGCCGATCGTGCTT3030.12783839202085917No Hit
GTCCCGTCAGACGCGGCAGTGCATATTGCAGCTGAGCCAGCTCAATTTGA3020.12741648313630188No Hit
GTTTCGTTTCCCCGGGACCTCTTGCCCCAATTCCTCCGCCTTGCCGGGAA3020.12741648313630188No Hit
GTCGAGACTGAAAAGCTATAACCCGCAGACCCGAGCGAAAGCGGCGGTCC2990.12615075648263002No Hit
GTTTGTATAGCCGACAAGCGCAATTTGAAGCACACCGTTTTTCTTTCTTC2970.12530693871351542No Hit
GGATAATTGCGCGGTTTTGACTGCGGAAGGAAGCCAAGGCCCACCTAAAG2970.12530693871351542No Hit
CCCAGGATGAGTCCCTCAGCGTGACGCAGTAAACTAGTAGCATCCCGATC2950.12446312094440085No Hit
CTATATTTCGTTTTTCTGAGACCTATCCGAGTTCAGTGCGACCGTACAGC2930.12361930317528627No Hit
CACGTTTATTGTAAGCCGTCAGCATCGGGATATCATCTGCTTCAAGCTCC2920.12319739429072897No Hit
GTTGAACCATGTTGATTTCCCTGCGTTTGTATAGCCGACAAGCGCAATTT2920.12319739429072897No Hit
CCTGATTTAAAATTGCCTCTGGTGATTTAACCTTTTATCCCTTATTAGAA2910.1227754854061717No Hit
GGCGGTTGAACCATGTTGATTTCCCTGCGTTTGTATAGCCGACAAGCGCA2910.1227754854061717No Hit
CAGCACTCGTCAGGCGGTTGAACCATGTTGATTTCCCTGCGTTTGTATAG2880.12150975875249981No Hit
GCCTGATTTAAAATTGCCTCTGGTGATTTAACCTTTTATCCCTTATTAGA2860.12066594098338523No Hit
CGGAAGATCCTGAATAAATCCTACTGTATCTGAAAGAAGAACACTGTAGC2830.11940021432971334No Hit
CTTTGGTATAGTTGGGATTTTAATATCATTAATAGCATGATGGTGATTGT2790.11771257879148418No Hit
GCGATTTCTGATATGGCGGCGGTCGGTTTCCAGTTTCGTTTCCCCGGGAC2790.11771257879148418No Hit
CCTGACCCCGGGGAACAGTCGCATGACGAGGCAGTTTCCAGAAACGTGTA2790.11771257879148418No Hit
GTTGGAACTCTACCAATTGGAGCTTTCTTAGCTGTCTTAGCAGTAGTTTA2770.11686876102236961No Hit
CGGACAACTGATGGACCCACGTTGCGAGTCCAGTAAATCAGGACGGGGAT2770.11686876102236961No Hit
GTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAACCAATGAGA2760.11644685213781233No Hit
GCCCTAACTGCGCAGTTAATAATTCTGGCAATTCGTCTCCACACTAGAAG2720.11475921659958314No Hit
CGGGGAACAGTCGCATGACGAGGCAGTTTCCAGAAACGTGTATCACATCT2710.11433730771502587No Hit
CGCATGACGAGGCAGTTTCCAGAAACGTGTATCACATCTAGGCATGGAAT2700.11391539883046857No Hit
ATTTCATGAATGCGATTTCTGATATGGCGGCGGTCGGTTTCCAGTTTCGT2690.11349348994591128No Hit
GGGATATTCTTTGGTATAGTTGGGATTTTAATATCATTAATAGCATGATG2660.11222776329223941No Hit
GTTGCCAATGACTTCAGCTGACTTGGCGACAGTTCATCATTAAAGATGAG2660.11222776329223941No Hit
CTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTT2640.11138394552312482No Hit
CGACAGTTCATCATTAAAGATGAGGAGATCAGCTTCAAGCTCTTCCACGA2610.11011821886945294No Hit
CTCGTTCTTTGGTCGCAATCCTGCGTAATCAACGCCGCAACTTTACGTCG2580.10885249221578108No Hit
CCGAGTTCAGTGCGACCGTACAGCTCTGGAACCCAAAGGTTCGTTTTTTT2540.10716485667755192No Hit
CGCAAGTTGTAGGTCGGGGGCACCACACCACGCTCGCTTGGCAGTAACGC2530.10674294779299462No Hit
GGGGAACAGTCGCATGACGAGGCAGTTTCCAGAAACGTGTATCACATCTA2520.10632103890843735No Hit
CGACGAACAACGAAGAGCGACGATGCCCGTTTCAGGTGGTCCTCAGCGTA2510.10589913002388004No Hit
CTTAGTTACAGTTTTATGGATAATTGGGATATTCTTTGGTATAGTTGGGA2500.10547722113932276No Hit
AATGACTTCAGCTGACTTGGCGACAGTTCATCATTAAAGATGAGGAGATC2490.10505531225476546No Hit
GCCCGTTTCAGGTGGTCCTCAGCGTACGGCGGGACCTCTGAGAATTGGGA2460.10378958560109358No Hit
GCGAATGACAGTGGAAAGCTGTGTGTTGATTTCATGAATGCGATTTCTGA2450.1033676767165363No Hit
CCCTGACCCCGGGGAACAGTCGCATGACGAGGCAGTTTCCAGAAACGTGT2440.102945767831979No Hit
ATCGGCTTCTATGGCGAATGACAGTGGAAAGCTGTGTGTTGATTTCATGA2420.10210195006286442No Hit
CAGGGATATTATTTTATTGCATTGTATTTCATCTTACCCAACCCCTTATG2400.10125813229374984No Hit
GCGGTCAATCATCTTCACTTCAATTGCTGTTGCCAATGACTTCAGCTGAC2390.10083622340919256No Hit
ATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGG2390.10083622340919256No Hit
CGCTATTAAAACCTTGAAAAGTATATTCAATATCCCTGTGGGATATTCTG2380.10041431452463526No Hit
GTTAACAGTTCTTGGCGCAAATAGCGCTGAATCGCTTCTTTAAAGGCTGC2380.10041431452463526No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)