FastQCFastQC Report
Wed 10 Aug 2022
EXCITED_1_R2.fq

Summary

[OK]Basic Statistics

MeasureValue
FilenameEXCITED_1_R2.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences237018
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCT16160.6818047574445822No Hit
CTCGTAACCAAACATGCACAGCGGTCAAACAGTATGTCCCAAGGGGACTT9720.4100954357896869No Hit
CTTAGATATTTTAAAGAGGCATCTATCACATAAGGCATCATTATAACTAA8340.3518720097207807No Hit
CAGAAATCGCATTCATGAAATCAACACACAGCTTTCCACTGTCATTCGCC8010.3379490165303901No Hit
CCTAACTTAATGGACGGGAGGTATCCCAATAGGAGGTTTCCTCCTATGGT7000.29533621919010367No Hit
CGAATAGCTCGTAACCAAACATGCACAGCGGTCAAACAGTATGTCCCAAG6890.2906952214599735No Hit
CAGCAATTGAAGTGAAGATGATTGACCGCACGCAATTGATATTAGATATT6690.2822570437688277No Hit
TGAAAATTCTATGGAAGAGCTAGCATCTCTGACGAAAACAGCAGACGGAA6490.27381886607768186No Hit
CGAAACTGGAAACCGACCGCCGCCATATCAGAAATCGCATTCATGAAATC6430.27128741277033813No Hit
CCCTAACTTAATGGACGGGAGGTATCCCAATAGGAGGTTTCCTCCTATGG6200.2615835084255204No Hit
GGAAGAGCTAGCATCTCTGACGAAAACAGCAGACGGAAAAGTACTGACCA6180.26073969065640584No Hit
CTCTCATTGGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCA6070.25609869292627563No Hit
CTAGCATCTCTGACGAAAACAGCAGACGGAAAAGTACTGACCAGCGTCAC6040.25483296627260377No Hit
CTTGAAGCTGATCTCCTCATCTTTAATGATGAACTGTCGCCAAGTCAGCT5980.25230151296526004No Hit
CAAAAACGGAACAGGGCTGACGCCGCTACATATATAGGAAAAGGGAAGGT5870.2476605152351298No Hit
GGCAAACTTCAAATTGAGCTGGCTCAGCTGCAATATGCACTGCCGCGTCT5560.23458133981385382No Hit
CCCAATTATCCATAAAACTGTAACTAAGTGAGGCTCTCTCATTGGTTTAT5470.2307841598528382No Hit
GGCCGATTGTCGCAGCCCGGGTCGATTATAACAACGGTGCAATCTCAGCT5410.22825270654549443No Hit
CATTAATTAGGGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAAT5360.22614316212270796No Hit
GTTTATTGGAAGCATCAACCTGCGCCGTCTTGTTAACTTGTCATATCGCG5340.22529934435359342No Hit
TATAGATTGGCGCTAGTAGATCACAGAACAACCGCCGCATACGGCCGATT5190.21897071108523403No Hit
CTTCAAATTGCGCTTGTCGGCTATACAAACGCAGGGAAATCAACATGGTT4980.2101106245095309No Hit
CAATTATCCATAAAACTGTAACTAAGTGAGGCTCTCTCATTGGTTTATAC4800.2025162645874997No Hit
CTTCCATTTCCGCCCAAGCTGCTCACAGTATACGGGCGTCGGCATCCAGA4770.20125053793382783No Hit
AAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTCTCTCA4760.2008286290492705No Hit
CTAACTTAATGGACGGGAGGTATCCCAATAGGAGGTTTCCTCCTATGGTT4760.2008286290492705No Hit
GAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTCTCTCATTG4740.19998481128015597No Hit
CTCATCTTTAATGATGAACTGTCGCCAAGTCAGCTGAAGTCATTGGCAAC4730.19956290239559865No Hit
GCTGAAGGCACTCGTGGAAGAGCTTGAAGCTGATCTCCTCATCTTTAATG4550.1919685424735674No Hit
CGGAAAAGTACTGACCAGCGTCACACAAAAACGGAACAGGGCTGACGCCG4540.1915466335890101No Hit
CGCGAATTGGGGACATTCAAATGAGCATCCTAGTCACCGCGTTTAAAATG4520.19070281581989554No Hit
GCAAACTTCAAATTGAGCTGGCTCAGCTGCAATATGCACTGCCGCGTCTG4500.18985899805078094No Hit
GGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGGAGGTTTC4440.1873275447434372No Hit
AGATGATTGACCGCACGCAATTGATATTAGATATTTTTGCAAAGCGGGCG4420.18648372697432264No Hit
GAAAAAACAGTGCTTCGGCTGCTTGAGGAGCTTGAAGCAGATGATATCCC4390.18521800032065075No Hit
CATTGATTGCTGCATTCCGCTCAACGCTTGAGGAAGTAAAAGAAGCGGAT4390.18521800032065075No Hit
GTTGGTTTATGCTCATCCCTACTCATAACCCCAGCACTTAGATATTTTAA4390.18521800032065075No Hit
CCTCGATCCATCGCGTATAGGGACGCCCCCTGCTCGCGTTACTGCCAAGC4380.18479609143609346No Hit
GGAAAAGGGAAGGTAGAAGAGCTGAAGGCACTCGTGGAAGAGCTTGAAGC4330.182686547013307No Hit
CTCAGCTAAACCGACGCAGTTTTGCTCCTTGGATTCTGAGCCCGGGCATC4310.18184272924419242No Hit
GGAAGGTAGAAGAGCTGAAGGCACTCGTGGAAGAGCTTGAAGCTGATCTC4260.17973318482140596No Hit
GGCTGACGCCGCTACATATATAGGAAAAGGGAAGGTAGAAGAGCTGAAGG4220.1780455492831768No Hit
GGGGAAACGAAACTGGAAACCGACCGCCGCCATATCAGAAATCGCATTCA4140.1746702782067185No Hit
GTAAAAGAAGCGGATTTAATTCTGCATTTAATTGATTCTTCAAATGAGGA4140.1746702782067185No Hit
GCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGGAGGTTTCC4110.1734045515530466No Hit
CATGGTTCAACCGCCTGACGAGTGCTGACAGCTATGAAGAAGACCTCCTG4110.1734045515530466No Hit
GCTGCTCACAGTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGG4080.17213882489937474No Hit
ATCAGAAATCGCATTCATGAAATCAACACACAGCTTTCCACTGTCATTCG4080.17213882489937474No Hit
GTCAAACAGTATGTCCCAAGGGGACTTAAGCGCGGTGGCCTCCCCTATCC3950.16665400940012995No Hit
GAGAAGGCAAACTTCAAATTGAGCTGGCTCAGCTGCAATATGCACTGCCG3870.16327873832367162No Hit
GAAAAACGGTGTGCTTCAAATTGCGCTTGTCGGCTATACAAACGCAGGGA3850.16243492055455702No Hit
GGGATAGATAGCCACCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTT3800.16032537613177056No Hit
ATTAGGGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGGAG3730.15737201393986955No Hit
GAACAACCGCCGCATACGGCCGATTGTCGCAGCCCGGGTCGATTATAACA3700.15610628728619766No Hit
CTTCAAATGAGGATTATGCGGGACATGAAAAAACAGTGCTTCGGCTGCTT3690.1556843784016404No Hit
CTGTAACTAAGTGAGGCTCTCTCATTGGTTTATACTTCAATATAAGCCTT3660.1544186517479685No Hit
GAAAATTCTATGGAAGAGCTAGCATCTCTGACGAAAACAGCAGACGGAAA3640.15357483397885394No Hit
ATTTATTCAGGATCTTCCGACGACATTGATTGCTGCATTCCGCTCAACGC3630.15315292509429662No Hit
GGGAAACGAAACTGGAAACCGACCGCCGCCATATCAGAAATCGCATTCAT3630.15315292509429662No Hit
CACAGAACAACCGCCGCATACGGCCGATTGTCGCAGCCCGGGTCGATTAT3530.14893383624872372No Hit
GTGAGGCTCTCTCATTGGTTTATACTTCAATATAAGCCTTGGTAGGGATA3520.14851192736416644No Hit
CTGTCATTCGCCATAGAAGCCGATACCGTGAAAGAAGAAAGAAAAACGGT3500.14766810959505183No Hit
ATTTTTGCAAAGCGGGCGAGAACGAGAGAAGGCAAACTTCAAATTGAGCT3490.14724620071049457No Hit
CTTCACTGCCCCCTTTCAGCCTTTTGGGTCCTGTATCCCAATTCTCAGAG3460.14598047405682268No Hit
AGCGGATTTAATTCTGCATTTAATTGATTCTTCAAATGAGGATTATGCGG3380.14260520298036436No Hit
GTTAACTTGTCATATCGCGCACGTAGTAGCCTAGAGCGCCAGGGGCGGAA3360.14176138521124979No Hit
CGTAACCAAACATGCACAGCGGTCAAACAGTATGTCCCAAGGGGACTTAA3340.1409175674421352No Hit
CGAGAGAAGGCAAACTTCAAATTGAGCTGGCTCAGCTGCAATATGCACTG3310.13965184078846332No Hit
GAAAGATCATGTTTATTGGAAGCATCAACCTGCGCCGTCTTGTTAACTTG3310.13965184078846332No Hit
CGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGGCTAGA3300.13922993190390603No Hit
CAAACATGCACAGCGGTCAAACAGTATGTCCCAAGGGGACTTAAGCGCGG3300.13922993190390603No Hit
CAGGAACATCCTCGTGTTAGATATTGAGGCTGCTTCGTGTCGGCACGAAG3260.13754229636567686No Hit
CCTGAAACGGGCATCGTCGCTCTTCGTTGTTCGTCGACTTCTAGTGTGGA3230.136276569712005No Hit
ATTAATTAGGGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATA3200.1350108430583331No Hit
AGAAGACCTCCTGTTTGCCACGCTGGACCCGATGACCAGAAAAATGGTCC3200.1350108430583331No Hit
CTGAGGACCACCTGAAACGGGCATCGTCGCTCTTCGTTGTTCGTCGACTT3190.13458893417377585No Hit
CTCTGACGAAAACAGCAGACGGAAAAGTACTGACCAGCGTCACACAAAAA3180.13416702528921853No Hit
CAATAACGTCTCTTTCAGAAGGCATTGGTATCTTTTCCCCACTTCCAAGC3140.13247938975098938No Hit
GTATGTCCCAAGGGGACTTAAGCGCGGTGGCCTCCCCTATCCCCTACGAG3090.13036984532820292No Hit
GATTTATTCAGGATCTTCCGACGACATTGATTGCTGCATTCCGCTCAACG3090.13036984532820292No Hit
GAGGAATTGGGGCAAGAGGTCCCGGGGAAACGAAACTGGAAACCGACCGC3020.12741648313630188No Hit
AGAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTCTCTCATT3010.1269945742517446No Hit
GTTAGGGCAGCGTCTGAGGAAGTTTGCTGCGGTTTCGCCTTGACCGCGGG2990.12615075648263002No Hit
GTCGCACTGAACTCGGATAGGTCTCAGAAAAACGAAATATAGGCTTACGG2990.12615075648263002No Hit
CTATGGAAGAGCTAGCATCTCTGACGAAAACAGCAGACGGAAAAGTACTG2980.1257288475980727No Hit
CTCATCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTA2970.12530693871351542No Hit
GGGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGGAGGTTT2970.12530693871351542No Hit
CTCTCTCATTGGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGC2950.12446312094440085No Hit
GGGAGGTATCCCAATAGGAGGTTTCCTCCTATGGTTTTCAAAACAATCAC2940.12404121205984356No Hit
CAGCGGTCAAACAGTATGTCCCAAGGGGACTTAAGCGCGGTGGCCTCCCC2900.1223535765216144No Hit
AGAGAAGGCAAACTTCAAATTGAGCTGGCTCAGCTGCAATATGCACTGCC2890.12193166763705711No Hit
CCCGGATCGATGACGCGAATTGGGGACATTCAAATGAGCATCCTAGTCAC2890.12193166763705711No Hit
GACGAATTGCCAGAATTATTAACTGCGCAGTTAGGGCAGCGTCTGAGGAA2860.12066594098338523No Hit
GTACTGACCAGCGTCACACAAAAACGGAACAGGGCTGACGCCGCTACATA2850.12024403209882793No Hit
GTCAAAACCGCGCAATTATCCCCGTCCTGATTTACTGGACTCGCAACGTG2810.11855639656059877No Hit
GGATAGATAGCCACCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTG2790.11771257879148418No Hit
AGAACAACCGCCGCATACGGCCGATTGTCGCAGCCCGGGTCGATTATAAC2780.11729066990692691No Hit
CAGCTTTCCACTGTCATTCGCCATAGAAGCCGATACCGTGAAAGAAGAAA2770.11686876102236961No Hit
GCCGATACCGTGAAAGAAGAAAGAAAAACGGTGTGCTTCAAATTGCGCTT2760.11644685213781233No Hit
CGCAGGGAAATCAACATGGTTCAACCGCCTGACGAGTGCTGACAGCTATG2760.11644685213781233No Hit
CACAGCTTTCCACTGTCATTCGCCATAGAAGCCGATACCGTGAAAGAAGA2760.11644685213781233No Hit
GCTCGAGTTGCCCCTTTGCCCGATCCCGGAAGATACGCTCTAAGCTCGGC2750.11602494325325503No Hit
CGGGACATGAAAAAACAGTGCTTCGGCTGCTTGAGGAGCTTGAAGCAGAT2740.11560303436869773No Hit
CCTTGACCGCGGGAAGGAGACATAACGATAGCGACTCTGTCTCAGGGGAT2730.11518112548414045No Hit
CTTCTTTCAGATACAGTAGGATTTATTCAGGATCTTCCGACGACATTGAT2710.11433730771502587No Hit
CAGGATCTTCCGACGACATTGATTGCTGCATTCCGCTCAACGCTTGAGGA2700.11391539883046857No Hit
GTCTCTTTCAGAAGGCATTGGTATCTTTTCCCCACTTCCAAGCATTTTTT2690.11349348994591128No Hit
CCGATACCGTGAAAGAAGAAAGAAAAACGGTGTGCTTCAAATTGCGCTTG2680.11307158106135398No Hit
CAACACACAGCTTTCCACTGTCATTCGCCATAGAAGCCGATACCGTGAAA2680.11307158106135398No Hit
CTGAACTCGGATAGGTCTCAGAAAAACGAAATATAGGCTTACGGTAGGTC2660.11222776329223941No Hit
GGAACATCCTCGTGTTAGATATTGAGGCTGCTTCGTGTCGGCACGAAGTG2650.11180585440768212No Hit
CTTATACAGAATTATCAATCAAGCTCCCCGAGGAGCGGACTTGTAAGGAC2630.11096203663856755No Hit
AAAACGAATAGCTCGTAACCAAACATGCACAGCGGTCAAACAGTATGTCC2600.10969630998489566No Hit
CTTTTCCCCACTTCCAAGCATTTTTTCAACTAATCTTATGTTATTAACCA2590.10927440110033837No Hit
GGGAAATCAACATGGTTCAACCGCCTGACGAGTGCTGACAGCTATGAAGA2580.10885249221578108No Hit
ATTACTTCCATTTCCGCCCAAGCTGCTCACAGTATACGGGCGTCGGCATC2580.10885249221578108No Hit
GAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGGAGGTTTCCTCC2540.10716485667755192No Hit
CAACATGGTTCAACCGCCTGACGAGTGCTGACAGCTATGAAGAAGACCTC2530.10674294779299462No Hit
GTCATTGGCAACAGCAATTGAAGTGAAGATGATTGACCGCACGCAATTGA2520.10632103890843735No Hit
GAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATACAGTA2510.10589913002388004No Hit
AAAAAAACGAACCTTTGGGTTCCAGAGCTGTACGGTCGCACTGAACTCGG2500.10547722113932276No Hit
GCATCTCTGACGAAAACAGCAGACGGAAAAGTACTGACCAGCGTCACACA2490.10505531225476546No Hit
AATCAACACACAGCTTTCCACTGTCATTCGCCATAGAAGCCGATACCGTG2490.10505531225476546No Hit
ATTTAATTCTGCATTTAATTGATTCTTCAAATGAGGATTATGCGGGACAT2460.10378958560109358No Hit
ATTGATTGCTGCATTCCGCTCAACGCTTGAGGAAGTAAAAGAAGCGGATT2420.10210195006286442No Hit
GTTCTTCTTTCAGATACAGTAGGATTTATTCAGGATCTTCCGACGACATT2410.10168004117830715No Hit
GCTGAAGTCATTGGCAACAGCAATTGAAGTGAAGATGATTGACCGCACGC2400.10125813229374984No Hit
CGACGACATTGATTGCTGCATTCCGCTCAACGCTTGAGGAAGTAAAAGAA2390.10083622340919256No Hit
CTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGGAGGTTTCCT2390.10083622340919256No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)