FastQCFastQC Report
Wed 10 Aug 2022
EXCITED_2_R1.fq

Summary

[OK]Basic Statistics

MeasureValue
FilenameEXCITED_2_R1.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences158009
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCAGAATTCAAGACGTTAACAGTTCTTGGCGCAAATAGCGCTGAATCGCT16891.068926453556443No Hit
CGCTTTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAAT11230.710719009676664No Hit
CTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGT10760.6809738685771063No Hit
CCGCTTTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAA10490.6638862343284244No Hit
CTTTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATAT10160.6430013480244796No Hit
CTCAATTTGAAGTTTGCCTTCTCTCGTTCTCGCCCGCTTTGCAAAAATAT7320.4632647507420463No Hit
GCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGA6260.3961799644324058No Hit
GTTGATTTCATGAATGCGATTTCTGATATGGCGGCGGTCGGTTTCCAGTT6020.38099095621135504No Hit
CTTCTCTCGTTCTCGCCCGCTTTGCAAAAATATCTAATATCAATTGCGTG5680.35947319456486654No Hit
CTGAGACAGAGTCGCTATCGTTATGTCTCCTTCCCGCGGTCAAGGCGAAA5490.34744856305653476No Hit
GCCTTCTCTCGTTCTCGCCCGCTTTGCAAAAATATCTAATATCAATTGCG5390.34111980963109695No Hit
CTGCGTTTGTATAGCCGACAAGCGCAATTTGAAGCACACCGTTTTTCTTT5280.3341581808631154No Hit
CTCAAATTTCGCACTGACCATAATGTGATCCCTTCCGGCGGTCGGTATAA5170.3271965520951338No Hit
CAGAATTCAAGACGTTAACAGTTCTTGGCGCAAATAGCGCTGAATCGCTT4920.31137466853153933No Hit
CGGCTGTCGAGTTGTACGGCCGTTCAGCCACGAGTCACGGGGTCTAACGC4750.3006157877082951No Hit
GTCGGAAGATCCTGAATAAATCCTACTGTATCTGAAAGAAGAACACTGTA4730.29935003702320756No Hit
CCCATTTTTAGTTATAATGATGCCTTATGTGATAGATGCCTCTTTAAAAT4670.29555278496794485No Hit
CTTCACTTCAATTGCTGTTGCCAATGACTTCAGCTGACTTGGCGACAGTT4600.2911226575701384No Hit
CTCAAGCGTTGAGCGGAATGCAGCAATCAATGTCGTCGGAAGATCCTGAA4390.277832275376719No Hit
CCGCATAATCCTCATTTGAAGAATCAATTAAATGCAGAATTAAATCCGCT4380.27719940003417526No Hit
CTCAGACGCTGCCCTAACTGCGCAGTTAATAATTCTGGCAATTCGTCTCC4350.27530077400654396No Hit
CGCCAATCTATATTACTCACAATATCCTAAAAACGATCAGCCGGCAGGTT4320.2734021479789126No Hit
CTGTGATCTACTAGCGCCAATCTATATTACTCACAATATCCTAAAAACGA4320.2734021479789126No Hit
GGAAGCTATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTG4260.26960489592364995No Hit
CTCGTTCTCGCCCGCTTTGCAAAAATATCTAATATCAATTGCGTGCGGTC4190.26517476852584343No Hit
CCGGGATCGGGCAAAGGGGCAACTCGAGCTAATCTCCCCAGCGGCTAGCA4110.2601117657854932No Hit
CTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGGGATGAGCATAAACCA4070.2575802644153181No Hit
CAATGACTTCAGCTGACTTGGCGACAGTTCATCATTAAAGATGAGGAGAT3910.24745425893461767No Hit
CTTCTTCATAGCTGTCAGCACTCGTCAGGCGGTTGAACCATGTTGATTTC3830.2423912561942674No Hit
CTCGGCAATAGGATTGTAGCCCAGGATGAGTCCCTCAGCGTGACGCAGTA3800.24049263016663608No Hit
CTCTCGTTCTCGCCCGCTTTGCAAAAATATCTAATATCAATTGCGTGCGG3780.2392268794815485No Hit
GTCAGCACTCGTCAGGCGGTTGAACCATGTTGATTTCCCTGCGTTTGTAT3680.23289812605611074No Hit
ATCGGGTCCAGCGTGGCAAACAGGAGGTCTTCTTCATAGCTGTCAGCACT3570.22593649728812915No Hit
CCGGGACCTCTTGCCCCAATTCCTCCGCCTTGCCGGGAAAGGTTGATCCC3540.22403787126049785No Hit
CCCGCATAATCCTCATTTGAAGAATCAATTAAATGCAGAATTAAATCCGC3500.2215063698903227No Hit
AGGAGATGTTGGAACTCTACCAATTGGAGCTTTCTTAGCTGTCTTAGCAG3480.22024061920523516No Hit
CTGCGATAGGCTAGTTCATAAACGAGGGGCGATGCCCGGGCTCAGAATCC3430.21707624249251625No Hit
CCCAATTCCTCCGCCTTGCCGGGAAAGGTTGATCCCTTGTCCCGTCAGAC3420.21644336714997248No Hit
GTTATGAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAA3370.21327899043725357No Hit
GTAAAACGCAAGCACCGGCTGTCGAGTTGTACGGCCGTTCAGCCACGAGT3320.2101146137245347No Hit
CGCATAATCCTCATTTGAAGAATCAATTAAATGCAGAATTAAATCCGCTT3270.20695023701181578No Hit
CTTTGGTATAGTTGGGATTTTAATATCATTAATAGCATGATGGTGATTGT3130.1980899822162029No Hit
CGCTTCTTTAAAGGCTGCAGCGTCGTCCTCAAATTTCGCACTGACCATAA3120.1974571068736591No Hit
GTCCAGCGTGGCAAACAGGAGGTCTTCTTCATAGCTGTCAGCACTCGTCA3060.19365985481839643No Hit
CCACATTCAAATTGACCTCCCTCAGGAAGCTAAGAAATACTATCTCGGCA3060.19365985481839643No Hit
GCCTCACTTAGTTACAGTTTTATGGATAATTGGGATATTCTTTGGTATAG3060.19365985481839643No Hit
GTCGGTTTAGCTGAGATTGCACCGTTGTTATAATCGACCCGGGCTGCGAC3030.19176122879076507No Hit
GGTCAATCATCTTCACTTCAATTGCTGTTGCCAATGACTTCAGCTGACTT3030.19176122879076507No Hit
GTCGTCATTGCGCCAGCTCGTCAGCGCTGGTCGTCTACCAGCTCGATAAT2950.18669822605041486No Hit
CACGTTTATTGTAAGCCGTCAGCATCGGGATATCATCTGCTTCAAGCTCC2940.18606535070787106No Hit
CTCACTTAGTTACAGTTTTATGGATAATTGGGATATTCTTTGGTATAGTT2940.18606535070787106No Hit
CTGGGGTTATGAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGAT2900.18353384933769595No Hit
CGCAAGTTGTAGGTCGGGGGCACCACACCACGCTCGCTTGGCAGTAACGC2810.17783797125480194No Hit
GGAAGATCCTGAATAAATCCTACTGTATCTGAAAGAAGAACACTGTAGCC2810.17783797125480194No Hit
CGAGGATGTTCCTGTTGATACCGTCTGCGATAGGCTAGTTCATAAACGAG2800.17720509591225816No Hit
CTCCTTCCCGCGGTCAAGGCGAAACCGCAGCAAACTTCCTCAGACGCTGC2790.17657222056971436No Hit
CCGAGTTCAGTGCGACCGTACAGCTCTGGAACCCAAAGGTTCGTTTTTTT2790.17657222056971436No Hit
GCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGGGATGAGCATAA2790.17657222056971436No Hit
GTCAGCATCGGGATATCATCTGCTTCAAGCTCCTCAAGCAGCCGAAGCAC2770.17530646988462684No Hit
CCTGATTTAAAATTGCCTCTGGTGATTTAACCTTTTATCCCTTATTAGAA2750.17404071919953926No Hit
CAGCAATCAATGTCGTCGGAAGATCCTGAATAAATCCTACTGTATCTGAA2720.17214209317190793No Hit
CGGCAGGTTCATTTTAAACGCGGTGACTAGGATGCTCATTTGAATGTCCC2710.17150921782936415No Hit
CTCAGCGTGACGCAGTAAACTAGTAGCATCCCGATCTTTTCCCTATCTAA2680.16961059180173282No Hit
CGACGAACAACGAAGAGCGACGATGCCCGTTTCAGGTGGTCCTCAGCGTA2670.16897771645918905No Hit
GTCGGGGGCACCACACCACGCTCGCTTGGCAGTAACGCGAGCAGGGGGCG2660.16834484111664527No Hit
GTCTGCGATAGGCTAGTTCATAAACGAGGGGCGATGCCCGGGCTCAGAAT2640.1670790904315577No Hit
ATCACATCTAGGCATGGAATCTTATGCCAGCTAACGGAACAAGCTTTGTG2640.1670790904315577No Hit
ATTTTTAGTTATAATGATGCCTTATGTGATAGATGCCTCTTTAAAATATC2610.16518046440392636No Hit
GGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATCGGGATATCATC2600.16454758906138256No Hit
ATCTAATTCACCTCCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAA2590.16391471371883878No Hit
CCTCACTTAGTTACAGTTTTATGGATAATTGGGATATTCTTTGGTATAGT2520.15948458632103235No Hit
CTTACAAGTCCGCTCCTCGGGGAGCTTGATTGATAATTCTGTATAAGGTG2520.15948458632103235No Hit
CGCATATAACCACATTCAAATTGACCTCCCTCAGGAAGCTAAGAAATACT2510.15885171097848858No Hit
GTCGGTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAG2500.15821883563594477No Hit
GTCTCCTTCCCGCGGTCAAGGCGAAACCGCAGCAAACTTCCTCAGACGCT2480.15695308495085725No Hit
CGCATGACGAGGCAGTTTCCAGAAACGTGTATCACATCTAGGCATGGAAT2440.15442158358068211No Hit
CCGATCTTTTCCCTATCTAATTCACCTCCTATTAGGAGCCGATCGTGCTT2430.15378870823813834No Hit
GTTGATACCGTCTGCGATAGGCTAGTTCATAAACGAGGGGCGATGCCCGG2430.15378870823813834No Hit
GTCGGTTTCCAGTTTCGTTTCCCCGGGACCTCTTGCCCCAATTCCTCCGC2400.15189008221050698No Hit
GTCTTCTTCATAGCTGTCAGCACTCGTCAGGCGGTTGAACCATGTTGATT2380.15062433152541946No Hit
GGCAGGTTCATTTTAAACGCGGTGACTAGGATGCTCATTTGAATGTCCCC2380.15062433152541946No Hit
GTTTTTTCATGTCCCGCATAATCCTCATTTGAAGAATCAATTAAATGCAG2370.14999145618287565No Hit
CAATCATCTTCACTTCAATTGCTGTTGCCAATGACTTCAGCTGACTTGGC2370.14999145618287565No Hit
CGGAAGATCCTGAATAAATCCTACTGTATCTGAAAGAAGAACACTGTAGC2300.1455613287850692No Hit
CCCGTTTCAGGTGGTCCTCAGCGTACGGCGGGACCTCTGAGAATTGGGAT2290.14492845344252542No Hit
CGATAATTGGCGCAAGTTGTAGGTCGGGGGCACCACACCACGCTCGCTTG2290.14492845344252542No Hit
GGGATAATTGCGCGGTTTTGACTGCGGAAGGAAGCCAAGGCCCACCTAAA2270.14366270275743787No Hit
CTTCTTTAAAGGCTGCAGCGTCGTCCTCAAATTTCGCACTGACCATAATG2270.14366270275743787No Hit
CCTGTTGATACCGTCTGCGATAGGCTAGTTCATAAACGAGGGGCGATGCC2270.14366270275743787No Hit
GTCCCGCATAATCCTCATTTGAAGAATCAATTAAATGCAGAATTAAATCC2250.1423969520723503No Hit
CTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTT2210.13986545070217518No Hit
GTCAGACGCGGCAGTGCATATTGCAGCTGAGCCAGCTCAATTTGAAGTTT2210.13986545070217518No Hit
GTGCATATTGCAGCTGAGCCAGCTCAATTTGAAGTTTGCCTTCTCTCGTT2210.13986545070217518No Hit
GGATATTCTTTGGTATAGTTGGGATTTTAATATCATTAATAGCATGATGG2200.1392325753596314No Hit
CTCGTTCTTTGGTCGCAATCCTGCGTAATCAACGCCGCAACTTTACGTCG2190.13859970001708763No Hit
GTGCGGTCAATCATCTTCACTTCAATTGCTGTTGCCAATGACTTCAGCTG2170.13733394933200008No Hit
GGGGTTATGAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGG2170.13733394933200008No Hit
GCCTGATTTAAAATTGCCTCTGGTGATTTAACCTTTTATCCCTTATTAGA2160.1367010739894563No Hit
CTTCTATGGCGAATGACAGTGGAAAGCTGTGTGTTGATTTCATGAATGCG2150.13606819864691252No Hit
CGCTATTAAAACCTTGAAAAGTATATTCAATATCCCTGTGGGATATTCTG2140.13543532330436875No Hit
CCCAGGATGAGTCCCTCAGCGTGACGCAGTAAACTAGTAGCATCCCGATC2140.13543532330436875No Hit
GTCAATCATCTTCACTTCAATTGCTGTTGCCAATGACTTCAGCTGACTTG2130.13480244796182497No Hit
GCCAGCTCAATTTGAAGTTTGCCTTCTCTCGTTCTCGCCCGCTTTGCAAA2110.1335366972767374No Hit
CGGACAACTGATGGACCCACGTTGCGAGTCCAGTAAATCAGGACGGGGAT2110.1335366972767374No Hit
GTTGATTTCCCTGCGTTTGTATAGCCGACAAGCGCAATTTGAAGCACACC2060.1303723205640185No Hit
CTTAGCAGTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGATGC2060.1303723205640185No Hit
CGGGGAACAGTCGCATGACGAGGCAGTTTCCAGAAACGTGTATCACATCT2030.12847369453638716No Hit
CCCGGGACCTCTTGCCCCAATTCCTCCGCCTTGCCGGGAAAGGTTGATCC2020.12784081919384338No Hit
GGGATATTCTTTGGTATAGTTGGGATTTTAATATCATTAATAGCATGATG2020.12784081919384338No Hit
CGGCAATAGGATTGTAGCCCAGGATGAGTCCCTCAGCGTGACGCAGTAAA2010.1272079438512996No Hit
CTTAGTTACAGTTTTATGGATAATTGGGATATTCTTTGGTATAGTTGGGA2000.12657506850875583No Hit
CCACGAGTGCCTTCAGCTCTTCTACCTTCCCTTTTCCTATATATGTAGCG1970.1246764424811245No Hit
GTTTATTGTAAGCCGTCAGCATCGGGATATCATCTGCTTCAAGCTCCTCA1970.1246764424811245No Hit
TTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTA1970.1246764424811245No Hit
GTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGATGCCTTATGT1960.12404356713858072No Hit
GTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGGGA1950.12341069179603693No Hit
GCCCTAACTGCGCAGTTAATAATTCTGGCAATTCGTCTCCACACTAGAAG1940.12277781645349316No Hit
CTGATTTAAAATTGCCTCTGGTGATTTAACCTTTTATCCCTTATTAGAAA1920.12151206576840559No Hit
CTAGTTCATAAACGAGGGGCGATGCCCGGGCTCAGAATCCAAGGAGCAAA1910.12087919042586182No Hit
GGCAGTTTCCAGAAACGTGTATCACATCTAGGCATGGAATCTTATGCCAG1900.12024631508331804No Hit
TCTCGTTCTCGCCCGCTTTGCAAAAATATCTAATATCAATTGCGTGCGGT1900.12024631508331804No Hit
GCCATTCGGACCTACCGTAAGCCTATATTTCGTTTTTCTGAGACCTATCC1880.11898056439823047No Hit
CACTTAGTTACAGTTTTATGGATAATTGGGATATTCTTTGGTATAGTTGG1870.11834768905568671No Hit
GTCGAGACTGAAAAGCTATAACCCGCAGACCCGAGCGAAAGCGGCGGTCC1860.11771481371314294No Hit
CTACTGTATCTGAAAGAAGAACACTGTAGCCGCTTGGCAGGACCATTTTT1850.11708193837059915No Hit
TCCGCTTTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAA1840.11644906302805537No Hit
CGACAGTTCATCATTAAAGATGAGGAGATCAGCTTCAAGCTCTTCCACGA1840.11644906302805537No Hit
GTGGAAAGCTGTGTGTTGATTTCATGAATGCGATTTCTGATATGGCGGCG1840.11644906302805537No Hit
CTCGTCAGGCGGTTGAACCATGTTGATTTCCCTGCGTTTGTATAGCCGAC1820.1151833123429678No Hit
CCCAGCGGCTAGCATGTGTAGTGCGCCATATCATATCCAAGATAGTGTAG1800.11391756165788024No Hit
GTTTGTATAGCCGACAAGCGCAATTTGAAGCACACCGTTTTTCTTTCTTC1790.11328468631533646No Hit
TTCCGCTTTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACA1790.11328468631533646No Hit
CTCATTTGAATGTCCCCAATTCGCGTCATCGATCCGGGTAGCCTCGTAGG1770.11201893563024892No Hit
CTATATTTCGTTTTTCTGAGACCTATCCGAGTTCAGTGCGACCGTACAGC1760.11138606028770513No Hit
CCCGTCAGACGCGGCAGTGCATATTGCAGCTGAGCCAGCTCAATTTGAAG1730.10948743426007379No Hit
CTCGATAATTGGCGCAAGTTGTAGGTCGGGGGCACCACACCACGCTCGCT1700.10758880823244245No Hit
ATCGAGGTCATGACTGGACACTGCATCGGAAGACACTTCGTGCCGACACG1690.10695593288989867No Hit
CAGGGATATTATTTTATTGCATTGTATTTCATCTTACCCAACCCCTTATG1690.10695593288989867No Hit
CTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTA1660.10505730686226734No Hit
CCGGCAGGTTCATTTTAAACGCGGTGACTAGGATGCTCATTTGAATGTCC1640.10379155617717978No Hit
CTTTTCCTATATATGTAGCGGCGTCAGCCCTGTTCCGTTTTTGTGTGACG1640.10379155617717978No Hit
GTTTTTCTGAGACCTATCCGAGTTCAGTGCGACCGTACAGCTCTGGAACC1630.103158680834636No Hit
CTAAAAACGATCAGCCGGCAGGTTCATTTTAAACGCGGTGACTAGGATGC1620.10252580549209223No Hit
GCCGTCAGCATCGGGATATCATCTGCTTCAAGCTCCTCAAGCAGCCGAAG1620.10252580549209223No Hit
CCAGGAGATGTTGGAACTCTACCAATTGGAGCTTTCTTAGCTGTCTTAGC1620.10252580549209223No Hit
CCAGCGGCTAGCATGTGTAGTGCGCCATATCATATCCAAGATAGTGTAGG1620.10252580549209223No Hit
ATCGGCTTCTATGGCGAATGACAGTGGAAAGCTGTGTGTTGATTTCATGA1620.10252580549209223No Hit
GTTGGAACTCTACCAATTGGAGCTTTCTTAGCTGTCTTAGCAGTAGTTTA1600.10126005480700466No Hit
GTTTATAAGGAATATATCCCATTTTTAGTTATAATGATGCCTTATGTGAT1600.10126005480700466No Hit
ATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACCGC1600.10126005480700466No Hit
CTTTACAGCAAATACCAGCGGCCCTGACCCCGGGGAACAGTCGCATGACG1590.10062717946446088No Hit
CTTGGTATACGGACAACTGATGGACCCACGTTGCGAGTCCAGTAAATCAG1590.10062717946446088No Hit
CGTCAGACGCGGCAGTGCATATTGCAGCTGAGCCAGCTCAATTTGAAGTT1590.10062717946446088No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)