FastQCFastQC Report
Wed 10 Aug 2022
EXCITED_2_R2.fq

Summary

[OK]Basic Statistics

MeasureValue
FilenameEXCITED_2_R2.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences158009
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCT13190.8347625768152447No Hit
CTCGTAACCAAACATGCACAGCGGTCAAACAGTATGTCCCAAGGGGACTT7790.49300989184160393No Hit
CTTAGATATTTTAAAGAGGCATCTATCACATAAGGCATCATTATAACTAA7220.45693599731660856No Hit
CGAATAGCTCGTAACCAAACATGCACAGCGGTCAAACAGTATGTCCCAAG6290.3980785904600371No Hit
CCTAACTTAATGGACGGGAGGTATCCCAATAGGAGGTTTCCTCCTATGGT5780.36580194799030435No Hit
TGAAAATTCTATGGAAGAGCTAGCATCTCTGACGAAAACAGCAGACGGAA5510.3487143137416223No Hit
CTAGCATCTCTGACGAAAACAGCAGACGGAAAAGTACTGACCAGCGTCAC5330.3373225575758343No Hit
CCCTAACTTAATGGACGGGAGGTATCCCAATAGGAGGTTTCCTCCTATGG5020.31770342195697715No Hit
CAGAAATCGCATTCATGAAATCAACACACAGCTTTCCACTGTCATTCGCC4950.3132732945591707No Hit
CGAAACTGGAAACCGACCGCCGCCATATCAGAAATCGCATTCATGAAATC4800.30378016442101397No Hit
CAAAAACGGAACAGGGCTGACGCCGCTACATATATAGGAAAAGGGAAGGT4760.3012486630508389No Hit
CCCAATTATCCATAAAACTGTAACTAAGTGAGGCTCTCTCATTGGTTTAT4730.29935003702320756No Hit
CTCTCATTGGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCA4360.2759336493490877No Hit
CTTGAAGCTGATCTCCTCATCTTTAATGATGAACTGTCGCCAAGTCAGCT4320.2734021479789126No Hit
CTCATCTTTAATGATGAACTGTCGCCAAGTCAGCTGAAGTCATTGGCAAC4270.2702377712661937No Hit
CTTCCATTTCCGCCCAAGCTGCTCACAGTATACGGGCGTCGGCATCCAGA4220.2670733945534748No Hit
CTAACTTAATGGACGGGAGGTATCCCAATAGGAGGTTTCCTCCTATGGTT4020.2544158877025992No Hit
CAGCAATTGAAGTGAAGATGATTGACCGCACGCAATTGATATTAGATATT3920.24808713427716145No Hit
GGAAGAGCTAGCATCTCTGACGAAAACAGCAGACGGAAAAGTACTGACCA3900.24682138359207387No Hit
CTTCAAATTGCGCTTGTCGGCTATACAAACGCAGGGAAATCAACATGGTT3810.24112550550917985No Hit
TATAGATTGGCGCTAGTAGATCACAGAACAACCGCCGCATACGGCCGATT3620.22910087400084805No Hit
CATTAATTAGGGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAAT3610.22846799865830428No Hit
CGGAAAAGTACTGACCAGCGTCACACAAAAACGGAACAGGGCTGACGCCG3580.22656937263067292No Hit
CTCTCTCATTGGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGC3520.22277212057541027No Hit
CTGTAACTAAGTGAGGCTCTCTCATTGGTTTATACTTCAATATAAGCCTT3500.2215063698903227No Hit
CGACGACATTGATTGCTGCATTCCGCTCAACGCTTGAGGAAGTAAAAGAA3370.21327899043725357No Hit
GGCCGATTGTCGCAGCCCGGGTCGATTATAACAACGGTGCAATCTCAGCT3240.20505161098418445No Hit
TTATAAACTACTGCTAAGACAGCTAAGAAAGCTCCAATTGGTAGAGTTCC3200.20252010961400932No Hit
CCTCGATCCATCGCGTATAGGGACGCCCCCTGCTCGCGTTACTGCCAAGC3200.20252010961400932No Hit
GTTTATTGGAAGCATCAACCTGCGCCGTCTTGTTAACTTGTCATATCGCG3200.20252010961400932No Hit
ATTTATTCAGGATCTTCCGACGACATTGATTGCTGCATTCCGCTCAACGC3180.20125435892892177No Hit
GAAAAAACAGTGCTTCGGCTGCTTGAGGAGCTTGAAGCAGATGATATCCC3160.1999886082438342No Hit
CATGGTTCAACCGCCTGACGAGTGCTGACAGCTATGAAGAAGACCTCCTG3120.1974571068736591No Hit
CAATTATCCATAAAACTGTAACTAAGTGAGGCTCTCTCATTGGTTTATAC3100.19619135618857156No Hit
CAACCTTTCCCGGCAAGGCGGAGGAATTGGGGCAAGAGGTCCCGGGGAAA3040.19239410413330887No Hit
GAAAATTCTATGGAAGAGCTAGCATCTCTGACGAAAACAGCAGACGGAAA3020.1911283534482213No Hit
CGCAGGGAAATCAACATGGTTCAACCGCCTGACGAGTGCTGACAGCTATG2980.1885968520780462No Hit
CTCTGACGAAAACAGCAGACGGAAAAGTACTGACCAGCGTCACACAAAAA2960.18733110139295864No Hit
AGATGATTGACCGCACGCAATTGATATTAGATATTTTTGCAAAGCGGGCG2900.18353384933769595No Hit
CTCAGCTAAACCGACGCAGTTTTGCTCCTTGGATTCTGAGCCCGGGCATC2850.18036947262497707No Hit
CATTGATTGCTGCATTCCGCTCAACGCTTGAGGAAGTAAAAGAAGCGGAT2810.17783797125480194No Hit
CGCGAATTGGGGACATTCAAATGAGCATCCTAGTCACCGCGTTTAAAATG2780.1759393452271706No Hit
GGAAAAGGGAAGGTAGAAGAGCTGAAGGCACTCGTGGAAGAGCTTGAAGC2760.17467359454208306No Hit
CACAGAACAACCGCCGCATACGGCCGATTGTCGCAGCCCGGGTCGATTAT2730.1727749685144517No Hit
CTTCACTGCCCCCTTTCAGCCTTTTGGGTCCTGTATCCCAATTCTCAGAG2720.17214209317190793No Hit
GTTGGTTTATGCTCATCCCTACTCATAACCCCAGCACTTAGATATTTTAA2700.17087634248682038No Hit
CCCGGATCGATGACGCGAATTGGGGACATTCAAATGAGCATCCTAGTCAC2660.16834484111664527No Hit
CTTCAAATGAGGATTATGCGGGACATGAAAAAACAGTGCTTCGGCTGCTT2650.16771196577410147No Hit
AAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTCTCTCA2630.16644621508901392No Hit
GGCAAACTTCAAATTGAGCTGGCTCAGCTGCAATATGCACTGCCGCGTCT2630.16644621508901392No Hit
CTGAACTCGGATAGGTCTCAGAAAAACGAAATATAGGCTTACGGTAGGTC2610.16518046440392636No Hit
CTGTCATTCGCCATAGAAGCCGATACCGTGAAAGAAGAAAGAAAAACGGT2600.16454758906138256No Hit
CTCATCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTA2590.16391471371883878No Hit
GCTGAAGGCACTCGTGGAAGAGCTTGAAGCTGATCTCCTCATCTTTAATG2540.1607503370061199No Hit
TTATAACTAAAAATGGGATATATTCCTTATAAACTACTGCTAAGACAGCT2510.15885171097848858No Hit
CTGAGGACCACCTGAAACGGGCATCGTCGCTCTTCGTTGTTCGTCGACTT2470.15632020960831347No Hit
CGCCGCTACATATATAGGAAAAGGGAAGGTAGAAGAGCTGAAGGCACTCG2470.15632020960831347No Hit
GTTAACTTGTCATATCGCGCACGTAGTAGCCTAGAGCGCCAGGGGCGGAA2460.15568733426576967No Hit
CCTGAAACGGGCATCGTCGCTCTTCGTTGTTCGTCGACTTCTAGTGTGGA2450.1550544589232259No Hit
GGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGGAGGTTTC2440.15442158358068211No Hit
CTATGGAAGAGCTAGCATCTCTGACGAAAACAGCAGACGGAAAAGTACTG2440.15442158358068211No Hit
GGGATAGATAGCCACCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTT2420.15315583289559456No Hit
GTAAAAGAAGCGGATTTAATTCTGCATTTAATTGATTCTTCAAATGAGGA2400.15189008221050698No Hit
ATCAGAAATCGCATTCATGAAATCAACACACAGCTTTCCACTGTCATTCG2370.14999145618287565No Hit
CAGGAACATCCTCGTGTTAGATATTGAGGCTGCTTCGTGTCGGCACGAAG2330.14745995481270055No Hit
CGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGGCTAGA2330.14745995481270055No Hit
CTTCTTTCAGATACAGTAGGATTTATTCAGGATCTTCCGACGACATTGAT2290.14492845344252542No Hit
GGAAGGTAGAAGAGCTGAAGGCACTCGTGGAAGAGCTTGAAGCTGATCTC2280.14429557809998167No Hit
CGAGAGAAGGCAAACTTCAAATTGAGCTGGCTCAGCTGCAATATGCACTG2260.1430298274148941No Hit
CAAAAACTGCCTGATTTTATACCGACCGCCGGAAGGGATCACATTATGGT2250.1423969520723503No Hit
CTTTTCCCCACTTCCAAGCATTTTTTCAACTAATCTTATGTTATTAACCA2230.14113120138726276No Hit
CGTAACCAAACATGCACAGCGGTCAAACAGTATGTCCCAAGGGGACTTAA2220.14049832604471896No Hit
ATTTTTGCAAAGCGGGCGAGAACGAGAGAAGGCAAACTTCAAATTGAGCT2210.13986545070217518No Hit
CTCCCCTATCCCCTACGAGGCTACCCGGATCGATGACGCGAATTGGGGAC2200.1392325753596314No Hit
CTAGCGTACGAGCACTATGGTCAGTAATTCCTGGAGGAATAGGTACCAAG2180.13796682467454385No Hit
ATTAGGGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGGAG2170.13733394933200008No Hit
ATTCCTTATAAACTACTGCTAAGACAGCTAAGAAAGCTCCAATTGGTAGA2170.13733394933200008No Hit
ATTAATTAGGGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATA2150.13606819864691252No Hit
GGCTGACGCCGCTACATATATAGGAAAAGGGAAGGTAGAAGAGCTGAAGG2140.13543532330436875No Hit
GAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTCTCTCATTG2140.13543532330436875No Hit
CAAACATGCACAGCGGTCAAACAGTATGTCCCAAGGGGACTTAAGCGCGG2110.1335366972767374No Hit
AAAAAAACGAACCTTTGGGTTCCAGAGCTGTACGGTCGCACTGAACTCGG2060.1303723205640185No Hit
GCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGGAGGTTTCC2060.1303723205640185No Hit
GAAAAACGGTGTGCTTCAAATTGCGCTTGTCGGCTATACAAACGCAGGGA2060.1303723205640185No Hit
CTATCACATAAGGCATCATTATAACTAAAAATGGGATATATTCCTTATAA2050.12973944522147474No Hit
CAACATGGTTCAACCGCCTGACGAGTGCTGACAGCTATGAAGAAGACCTC2050.12973944522147474No Hit
CGTGGTTTTACTAGGCTAGACTAGCGTACGAGCACTATGGTCAGTAATTC2050.12973944522147474No Hit
AGCGGATTTAATTCTGCATTTAATTGATTCTTCAAATGAGGATTATGCGG2040.12910656987893096No Hit
CTTTTGGGTCCTGTATCCCAATTCTCAGAGGTCCCGCCGTACGCTGAGGA2020.12784081919384338No Hit
AGAAGACCTCCTGTTTGCCACGCTGGACCCGATGACCAGAAAAATGGTCC2020.12784081919384338No Hit
CTTATACAGAATTATCAATCAAGCTCCCCGAGGAGCGGACTTGTAAGGAC2020.12784081919384338No Hit
CTAAAAATGGGATATATTCCTTATAAACTACTGCTAAGACAGCTAAGAAA2000.12657506850875583No Hit
CACAGCTTTCCACTGTCATTCGCCATAGAAGCCGATACCGTGAAAGAAGA2000.12657506850875583No Hit
GATTTATTCAGGATCTTCCGACGACATTGATTGCTGCATTCCGCTCAACG2000.12657506850875583No Hit
CTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGGAGGTTTCCT1980.12530931782366828No Hit
CGCTGGACCCGATGACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTT1970.1246764424811245No Hit
TATAACTAAAAATGGGATATATTCCTTATAAACTACTGCTAAGACAGCTA1950.12341069179603693No Hit
ATCACATAAGGCATCATTATAACTAAAAATGGGATATATTCCTTATAAAC1940.12277781645349316No Hit
CAACACACAGCTTTCCACTGTCATTCGCCATAGAAGCCGATACCGTGAAA1940.12277781645349316No Hit
CAGGATCTTCCGACGACATTGATTGCTGCATTCCGCTCAACGCTTGAGGA1930.12214494111094938No Hit
CTCGTGTTAGATATTGAGGCTGCTTCGTGTCGGCACGAAGTGTCTTCCGA1910.12087919042586182No Hit
GTGAGGCTCTCTCATTGGTTTATACTTCAATATAAGCCTTGGTAGGGATA1890.11961343974077425No Hit
CGCCTGACGAGTGCTGACAGCTATGAAGAAGACCTCCTGTTTGCCACGCT1890.11961343974077425No Hit
GTCAAACAGTATGTCCCAAGGGGACTTAAGCGCGGTGGCCTCCCCTATCC1870.11834768905568671No Hit
CAATAACGTCTCTTTCAGAAGGCATTGGTATCTTTTCCCCACTTCCAAGC1860.11771481371314294No Hit
CGCCCCTCGTTTATGAACTAGCCTATCGCAGACGGTATCAACAGGAACAT1850.11708193837059915No Hit
GGATAGATAGCCACCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTG1850.11708193837059915No Hit
CTCGTGGAAGAGCTTGAAGCTGATCTCCTCATCTTTAATGATGAACTGTC1840.11644906302805537No Hit
GTCGCACTGAACTCGGATAGGTCTCAGAAAAACGAAATATAGGCTTACGG1830.11581618768551158No Hit
GCAAACTTCAAATTGAGCTGGCTCAGCTGCAATATGCACTGCCGCGTCTG1820.1151833123429678No Hit
GAACAACCGCCGCATACGGCCGATTGTCGCAGCCCGGGTCGATTATAACA1800.11391756165788024No Hit
ATTTAATTCTGCATTTAATTGATTCTTCAAATGAGGATTATGCGGGACAT1800.11391756165788024No Hit
TGTTGGTTTATGCTCATCCCTACTCATAACCCCAGCACTTAGATATTTTA1800.11391756165788024No Hit
CCCAATTCTCAGAGGTCCCGCCGTACGCTGAGGACCACCTGAAACGGGCA1790.11328468631533646No Hit
CAGCGGTCAAACAGTATGTCCCAAGGGGACTTAAGCGCGGTGGCCTCCCC1780.11265181097279268No Hit
CTGTCGCCAAGTCAGCTGAAGTCATTGGCAACAGCAATTGAAGTGAAGAT1750.11075318494516136No Hit
CCAGCACTTAGATATTTTAAAGAGGCATCTATCACATAAGGCATCATTAT1740.11012030960261758No Hit
GTTCTTCTTTCAGATACAGTAGGATTTATTCAGGATCTTCCGACGACATT1740.11012030960261758No Hit
CGGGACATGAAAAAACAGTGCTTCGGCTGCTTGAGGAGCTTGAAGCAGAT1740.11012030960261758No Hit
CATCATTATAACTAAAAATGGGATATATTCCTTATAAACTACTGCTAAGA1740.11012030960261758No Hit
GAAAGATCATGTTTATTGGAAGCATCAACCTGCGCCGTCTTGTTAACTTG1740.11012030960261758No Hit
CTAGCCTATCGCAGACGGTATCAACAGGAACATCCTCGTGTTAGATATTG1730.10948743426007379No Hit
CCAAGCTGCTCACAGTATACGGGCGTCGGCATCCAGACCGTCGGCTGATC1720.10885455891753001No Hit
GCTGCTCACAGTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGG1720.10885455891753001No Hit
CCTTGACCGCGGGAAGGAGACATAACGATAGCGACTCTGTCTCAGGGGAT1710.10822168357498624No Hit
CTTGAAGCAGATGATATCCCGATGCTGACGGCTTACAATAAACGTGATCA1690.10695593288989867No Hit
CTCCTGTTTGCCACGCTGGACCCGATGACCAGAAAAATGGTCCTGCCAAG1690.10695593288989867No Hit
GTATGTCCCAAGGGGACTTAAGCGCGGTGGCCTCCCCTATCCCCTACGAG1690.10695593288989867No Hit
TCCATTTCCGCCCAAGCTGCTCACAGTATACGGGCGTCGGCATCCAGACC1680.10632305754735488No Hit
AGAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTCTCTCATT1670.10569018220481113No Hit
CTTGGCTTCCTTCCGCAGTCAAAACCGCGCAATTATCCCCGTCCTGATTT1670.10569018220481113No Hit
CGAGTGCTGACAGCTATGAAGAAGACCTCCTGTTTGCCACGCTGGACCCG1660.10505730686226734No Hit
CTCATTGGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACC1650.10442443151972357No Hit
CTTGGATATGATATGGCGCACTACACATGCTAGCCGCTGGGGAGATTAGC1650.10442443151972357No Hit
CTTATAAACTACTGCTAAGACAGCTAAGAAAGCTCCAATTGGTAGAGTTC1640.10379155617717978No Hit
CAGCTTTCCACTGTCATTCGCCATAGAAGCCGATACCGTGAAAGAAGAAA1640.10379155617717978No Hit
CACAAAGCTTGTTCCGTTAGCTGGCATAAGATTCCATGCCTAGATGTGAT1640.10379155617717978No Hit
CGTGATCAAAAACTGCCTGATTTTATACCGACCGCCGGAAGGGATCACAT1630.103158680834636No Hit
CACGTTTCTGGAAACTGCCTCGTCATGCGACTGTTCCCCGGGGTCAGGGC1610.10189293014954844No Hit
CTGAAACGGGCATCGTCGCTCTTCGTTGTTCGTCGACTTCTAGTGTGGAG1600.10126005480700466No Hit
CAACGGTGCAATCTCAGCTAAACCGACGCAGTTTTGCTCCTTGGATTCTG1590.10062717946446088No Hit
AGAGAAGGCAAACTTCAAATTGAGCTGGCTCAGCTGCAATATGCACTGCC1590.10062717946446088No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)