FastQCFastQC Report
Wed 10 Aug 2022
EXCITED_3_R1.fq

Summary

[OK]Basic Statistics

MeasureValue
FilenameEXCITED_3_R1.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences196673
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCAGAATTCAAGACGTTAACAGTTCTTGGCGCAAATAGCGCTGAATCGCT19761.0047134075343336No Hit
CTTTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATAT10200.5186273662373585No Hit
CTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGT10180.5176104498329716No Hit
CGCTTTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAAT9730.49472983073426446No Hit
CCGCTTTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAA9510.4835437502860077No Hit
GCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGA7830.3981227723175017No Hit
CTCAATTTGAAGTTTGCCTTCTCTCGTTCTCGCCCGCTTTGCAAAAATAT7790.39608893950872764No Hit
GTTGATTTCATGAATGCGATTTCTGATATGGCGGCGGTCGGTTTCCAGTT7000.3559207415354421No Hit
GCCTTCTCTCGTTCTCGCCCGCTTTGCAAAAATATCTAATATCAATTGCG6960.3538869087266681No Hit
CCGGGATCGGGCAAAGGGGCAACTCGAGCTAATCTCCCCAGCGGCTAGCA6000.3050749213160932No Hit
CTTCTCTCGTTCTCGCCCGCTTTGCAAAAATATCTAATATCAATTGCGTG5950.3025326303051258No Hit
CTGAGACAGAGTCGCTATCGTTATGTCTCCTTCCCGCGGTCAAGGCGAAA5780.29388884086783645No Hit
GTCGGAAGATCCTGAATAAATCCTACTGTATCTGAAAGAAGAACACTGTA5760.29287192446344945No Hit
CCCATTTTTAGTTATAATGATGCCTTATGTGATAGATGCCTCTTTAAAAT5530.2811773858129992No Hit
CGGCTGTCGAGTTGTACGGCCGTTCAGCCACGAGTCACGGGGTCTAACGC5510.2801604694086123No Hit
GGAAGCTATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTG5440.27660126199325785No Hit
CCGCATAATCCTCATTTGAAGAATCAATTAAATGCAGAATTAAATCCGCT5390.2740589709822904No Hit
CAGAATTCAAGACGTTAACAGTTCTTGGCGCAAATAGCGCTGAATCGCTT5370.2730420545779034No Hit
CTTCACTTCAATTGCTGTTGCCAATGACTTCAGCTGACTTGGCGACAGTT5280.268465930758162No Hit
CTGCGTTTGTATAGCCGACAAGCGCAATTTGAAGCACACCGTTTTTCTTT5260.26744901435377505No Hit
CTCAAATTTCGCACTGACCATAATGTGATCCCTTCCGGCGGTCGGTATAA5080.25829676671429225No Hit
CTGTGATCTACTAGCGCCAATCTATATTACTCACAATATCCTAAAAACGA4950.2516868100857769No Hit
CCGGGACCTCTTGCCCCAATTCCTCCGCCTTGCCGGGAAAGGTTGATCCC4920.2501614354791964No Hit
CGCCAATCTATATTACTCACAATATCCTAAAAACGATCAGCCGGCAGGTT4820.24507685345726157No Hit
CTCAGACGCTGCCCTAACTGCGCAGTTAATAATTCTGGCAATTCGTCTCC4700.23897535503093967No Hit
CTCGTTCTCGCCCGCTTTGCAAAAATATCTAATATCAATTGCGTGCGGTC4660.23694152222216575No Hit
CTCAAGCGTTGAGCGGAATGCAGCAATCAATGTCGTCGGAAGATCCTGAA4570.23236539840242434No Hit
ATCGGGTCCAGCGTGGCAAACAGGAGGTCTTCTTCATAGCTGTCAGCACT4510.22931464918926342No Hit
GCCTCACTTAGTTACAGTTTTATGGATAATTGGGATATTCTTTGGTATAG4480.22778927458268292No Hit
GTCAGCACTCGTCAGGCGGTTGAACCATGTTGATTTCCCTGCGTTTGTAT4380.22270469256074804No Hit
CAATGACTTCAGCTGACTTGGCGACAGTTCATCATTAAAGATGAGGAGAT4320.21965394334758712No Hit
GTAAAACGCAAGCACCGGCTGTCGAGTTGTACGGCCGTTCAGCCACGAGT4310.21914548514539361No Hit
CTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGGGATGAGCATAAACCA4170.21202707031468476No Hit
GTCCAGCGTGGCAAACAGGAGGTCTTCTTCATAGCTGTCAGCACTCGTCA4170.21202707031468476No Hit
CCCAATTCCTCCGCCTTGCCGGGAAAGGTTGATCCCTTGTCCCGTCAGAC4150.21101015391029782No Hit
GGTCAATCATCTTCACTTCAATTGCTGTTGCCAATGACTTCAGCTGACTT4080.2074509464949434No Hit
CTTCTTCATAGCTGTCAGCACTCGTCAGGCGGTTGAACCATGTTGATTTC4040.20541711368616944No Hit
GGAAGATCCTGAATAAATCCTACTGTATCTGAAAGAAGAACACTGTAGCC3970.201857906270815No Hit
CTCACTTAGTTACAGTTTTATGGATAATTGGGATATTCTTTGGTATAGTT3940.20033253166423454No Hit
CTCGGCAATAGGATTGTAGCCCAGGATGAGTCCCTCAGCGTGACGCAGTA3920.1993156152598476No Hit
GTTATGAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAA3830.19473949144010616No Hit
CTCTCGTTCTCGCCCGCTTTGCAAAAATATCTAATATCAATTGCGTGCGG3820.1942310332379127No Hit
AGGAGATGTTGGAACTCTACCAATTGGAGCTTTCTTAGCTGTCTTAGCAG3710.18863799301378428No Hit
CCACATTCAAATTGACCTCCCTCAGGAAGCTAAGAAATACTATCTCGGCA3690.18762107660939734No Hit
CCCGCATAATCCTCATTTGAAGAATCAATTAAATGCAGAATTAAATCCGC3680.18711261840720383No Hit
GTCTGCGATAGGCTAGTTCATAAACGAGGGGCGATGCCCGGGCTCAGAAT3630.18457032739623638No Hit
GTCTTCTTCATAGCTGTCAGCACTCGTCAGGCGGTTGAACCATGTTGATT3600.1830449527896559No Hit
CTGCGATAGGCTAGTTCATAAACGAGGGGCGATGCCCGGGCTCAGAATCC3570.18151957818307546No Hit
CCTCACTTAGTTACAGTTTTATGGATAATTGGGATATTCTTTGGTATAGT3540.17999420357649498No Hit
CAGCAATCAATGTCGTCGGAAGATCCTGAATAAATCCTACTGTATCTGAA3530.1794857453743015No Hit
CGCATAATCCTCATTTGAAGAATCAATTAAATGCAGAATTAAATCCGCTT3490.17745191256552756No Hit
GTCGGTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAG3470.17643499616114056No Hit
CGGCAATAGGATTGTAGCCCAGGATGAGTCCCTCAGCGTGACGCAGTAAA3450.1754180797567536No Hit
GTCGGGGGCACCACACCACGCTCGCTTGGCAGTAACGCGAGCAGGGGGCG3440.1749096215545601No Hit
GTCGGTTTAGCTGAGATTGCACCGTTGTTATAATCGACCCGGGCTGCGAC3390.17236733054359266No Hit
GGCAGGTTCATTTTAAACGCGGTGACTAGGATGCTCATTTGAATGTCCCC3370.17135041413920568No Hit
GTGCGGTCAATCATCTTCACTTCAATTGCTGTTGCCAATGACTTCAGCTG3360.17084195593701218No Hit
ATTTTTAGTTATAATGATGCCTTATGTGATAGATGCCTCTTTAAAATATC3320.16880812312823826No Hit
CGGCAGGTTCATTTTAAACGCGGTGACTAGGATGCTCATTTGAATGTCCC3310.16829966492604476No Hit
CTTTGGTATAGTTGGGATTTTAATATCATTAATAGCATGATGGTGATTGT3280.16677429031946428No Hit
GTCGTCATTGCGCCAGCTCGTCAGCGCTGGTCGTCTACCAGCTCGATAAT3250.16524891571288383No Hit
GTCGGTTTCCAGTTTCGTTTCCCCGGGACCTCTTGCCCCAATTCCTCCGC3240.16474045751069033No Hit
CGAGGATGTTCCTGTTGATACCGTCTGCGATAGGCTAGTTCATAAACGAG3210.16321508290410988No Hit
ATCTAATTCACCTCCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAA3190.1621981664997229No Hit
CTGGGGTTATGAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGAT3170.1611812500953359No Hit
GCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGGGATGAGCATAA3140.15965587548875546No Hit
GGGGTTATGAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGG3130.15914741728656195No Hit
GGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATCGGGATATCATC3070.15609666807340103No Hit
GGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGAGATGT3020.15355437706243358No Hit
CGCATATAACCACATTCAAATTGACCTCCCTCAGGAAGCTAAGAAATACT2970.15101208605146615No Hit
GCCATTCGGACCTACCGTAAGCCTATATTTCGTTTTTCTGAGACCTATCC2970.15101208605146615No Hit
CGCTTCTTTAAAGGCTGCAGCGTCGTCCTCAAATTTCGCACTGACCATAA2960.15050362784927265No Hit
ATCACATCTAGGCATGGAATCTTATGCCAGCTAACGGAACAAGCTTTGTG2930.14897825324269218No Hit
GTTTTTTCATGTCCCGCATAATCCTCATTTGAAGAATCAATTAAATGCAG2910.1479613368383052No Hit
GCCAGCTCAATTTGAAGTTTGCCTTCTCTCGTTCTCGCCCGCTTTGCAAA2910.1479613368383052No Hit
GTTGATACCGTCTGCGATAGGCTAGTTCATAAACGAGGGGCGATGCCCGG2900.14745287863611173No Hit
CCCAGGATGAGTCCCTCAGCGTGACGCAGTAAACTAGTAGCATCCCGATC2900.14745287863611173No Hit
CTCAGCGTGACGCAGTAAACTAGTAGCATCCCGATCTTTTCCCTATCTAA2890.14694442043391823No Hit
CGCAAGTTGTAGGTCGGGGGCACCACACCACGCTCGCTTGGCAGTAACGC2880.14643596223172473No Hit
GTCTCCTTCCCGCGGTCAAGGCGAAACCGCAGCAAACTTCCTCAGACGCT2840.1444021294229508No Hit
GTCAGACGCGGCAGTGCATATTGCAGCTGAGCCAGCTCAATTTGAAGTTT2830.1438936712207573No Hit
CGACGAACAACGAAGAGCGACGATGCCCGTTTCAGGTGGTCCTCAGCGTA2820.1433852130185638No Hit
CACGTTTATTGTAAGCCGTCAGCATCGGGATATCATCTGCTTCAAGCTCC2810.14287675481637033No Hit
GCCGTCAGCATCGGGATATCATCTGCTTCAAGCTCCTCAAGCAGCCGAAG2750.13982600560320937No Hit
GTCAGCATCGGGATATCATCTGCTTCAAGCTCCTCAAGCAGCCGAAGCAC2750.13982600560320937No Hit
CCTGTTGATACCGTCTGCGATAGGCTAGTTCATAAACGAGGGGCGATGCC2740.1393175474010159No Hit
CTTACAAGTCCGCTCCTCGGGGAGCTTGATTGATAATTCTGTATAAGGTG2730.13880908919882243No Hit
CAATCATCTTCACTTCAATTGCTGTTGCCAATGACTTCAGCTGACTTGGC2710.13779217279443542No Hit
GTCCCGCATAATCCTCATTTGAAGAATCAATTAAATGCAGAATTAAATCC2690.13677525639004845No Hit
CTTAGTTACAGTTTTATGGATAATTGGGATATTCTTTGGTATAGTTGGGA2670.13575833998566147No Hit
CCGATCTTTTCCCTATCTAATTCACCTCCTATTAGGAGCCGATCGTGCTT2650.1347414235812745No Hit
GCCCTAACTGCGCAGTTAATAATTCTGGCAATTCGTCTCCACACTAGAAG2640.134232965379081No Hit
GGGATAATTGCGCGGTTTTGACTGCGGAAGGAAGCCAAGGCCCACCTAAA2630.13372450717688752No Hit
GTGCATATTGCAGCTGAGCCAGCTCAATTTGAAGTTTGCCTTCTCTCGTT2630.13372450717688752No Hit
CCTGATTTAAAATTGCCTCTGGTGATTTAACCTTTTATCCCTTATTAGAA2620.13321604897469402No Hit
CCCGGGACCTCTTGCCCCAATTCCTCCGCCTTGCCGGGAAAGGTTGATCC2600.13219913257030708No Hit
CCCGTTTCAGGTGGTCCTCAGCGTACGGCGGGACCTCTGAGAATTGGGAT2600.13219913257030708No Hit
CGCATGACGAGGCAGTTTCCAGAAACGTGTATCACATCTAGGCATGGAAT2590.13169067436811357No Hit
CTCCTTCCCGCGGTCAAGGCGAAACCGCAGCAAACTTCCTCAGACGCTGC2560.1301652997615331No Hit
GTGGAAAGCTGTGTGTTGATTTCATGAATGCGATTTCTGATATGGCGGCG2530.12863992515495262No Hit
GTTTATTGTAAGCCGTCAGCATCGGGATATCATCTGCTTCAAGCTCCTCA2500.12711455054837217No Hit
CTCGTTCTTTGGTCGCAATCCTGCGTAATCAACGCCGCAACTTTACGTCG2500.12711455054837217No Hit
CTATATTTCGTTTTTCTGAGACCTATCCGAGTTCAGTGCGACCGTACAGC2460.1250807177395982No Hit
CGACAGTTCATCATTAAAGATGAGGAGATCAGCTTCAAGCTCTTCCACGA2430.12355534313301775No Hit
GTTGATTTCCCTGCGTTTGTATAGCCGACAAGCGCAATTTGAAGCACACC2410.12253842672863079No Hit
GGCGAATGACAGTGGAAAGCTGTGTGTTGATTTCATGAATGCGATTTCTG2400.12202996852643729No Hit
CGGACAACTGATGGACCCACGTTGCGAGTCCAGTAAATCAGGACGGGGAT2380.1210130521220503No Hit
CCGGCAGGTTCATTTTAAACGCGGTGACTAGGATGCTCATTTGAATGTCC2380.1210130521220503No Hit
CCGAGTTCAGTGCGACCGTACAGCTCTGGAACCCAAAGGTTCGTTTTTTT2370.12050459391985682No Hit
GGGATATTCTTTGGTATAGTTGGGATTTTAATATCATTAATAGCATGATG2360.11999613571766334No Hit
CGCTATTAAAACCTTGAAAAGTATATTCAATATCCCTGTGGGATATTCTG2350.11948767751546983No Hit
CGGAAGATCCTGAATAAATCCTACTGTATCTGAAAGAAGAACACTGTAGC2350.11948767751546983No Hit
GGCAGTTTCCAGAAACGTGTATCACATCTAGGCATGGAATCTTATGCCAG2340.11897921931327635No Hit
GCAGCAATCAATGTCGTCGGAAGATCCTGAATAAATCCTACTGTATCTGA2310.11745384470669588No Hit
CTACTGTATCTGAAAGAAGAACACTGTAGCCGCTTGGCAGGACCATTTTT2290.11643692830230891No Hit
GTCAATCATCTTCACTTCAATTGCTGTTGCCAATGACTTCAGCTGACTTG2290.11643692830230891No Hit
GTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGGGA2280.11592847010011542No Hit
GCCTGATTTAAAATTGCCTCTGGTGATTTAACCTTTTATCCCTTATTAGA2270.11542001189792192No Hit
GTTTGTATAGCCGACAAGCGCAATTTGAAGCACACCGTTTTTCTTTCTTC2270.11542001189792192No Hit
CTTCTTTAAAGGCTGCAGCGTCGTCCTCAAATTTCGCACTGACCATAATG2260.11491155369572845No Hit
TCCGCTTTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAA2240.11389463729134146No Hit
CGGGGAACAGTCGCATGACGAGGCAGTTTCCAGAAACGTGTATCACATCT2240.11389463729134146No Hit
CTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTT2230.11338617908914798No Hit
CTTCTATGGCGAATGACAGTGGAAAGCTGTGTGTTGATTTCATGAATGCG2230.11338617908914798No Hit
CCAGCGGCTAGCATGTGTAGTGCGCCATATCATATCCAAGATAGTGTAGG2230.11338617908914798No Hit
CCTGACCCCGGGGAACAGTCGCATGACGAGGCAGTTTCCAGAAACGTGTA2220.11287772088695448No Hit
GTGTACACCGCCCTTAGACGTCTTGGTATACGGACAACTGATGGACCCAC2200.11186080448256752No Hit
CGATAATTGGCGCAAGTTGTAGGTCGGGGGCACCACACCACGCTCGCTTG2190.11135234628037402No Hit
GGACAACTGATGGACCCACGTTGCGAGTCCAGTAAATCAGGACGGGGATA2170.11033542987598706No Hit
CTAGTTCATAAACGAGGGGCGATGCCCGGGCTCAGAATCCAAGGAGCAAA2170.11033542987598706No Hit
GTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGATGCCTTATGT2150.10931851347160007No Hit
TTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTA2110.1072846806628261No Hit
GGATATTCTTTGGTATAGTTGGGATTTTAATATCATTAATAGCATGATGG2090.10626776425843915No Hit
GTCGAGACTGAAAAGCTATAACCCGCAGACCCGAGCGAAAGCGGCGGTCC2090.10626776425843915No Hit
CCCTGACCCCGGGGAACAGTCGCATGACGAGGCAGTTTCCAGAAACGTGT2080.10575930605624564No Hit
CTCGTCAGGCGGTTGAACCATGTTGATTTCCCTGCGTTTGTATAGCCGAC2070.10525084785405216No Hit
CTTAGCAGTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGATGC2060.10474238965185866No Hit
GGGGATAATTGCGCGGTTTTGACTGCGGAAGGAAGCCAAGGCCCACCTAA2050.10423393144966518No Hit
CTTGTCCCGTCAGACGCGGCAGTGCATATTGCAGCTGAGCCAGCTCAATT1990.10118318223650426No Hit
CCCAGCGGCTAGCATGTGTAGTGCGCCATATCATATCCAAGATAGTGTAG1970.10016626583211727No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)