FastQCFastQC Report
Wed 10 Aug 2022
EXCITED_3_R2.fq

Summary

[OK]Basic Statistics

MeasureValue
FilenameEXCITED_3_R2.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences196673
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCT13920.7077738174533362No Hit
CTCGTAACCAAACATGCACAGCGGTCAAACAGTATGTCCCAAGGGGACTT8510.43269793006665885No Hit
CTTAGATATTTTAAAGAGGCATCTATCACATAAGGCATCATTATAACTAA7440.37829290243195557No Hit
CGAATAGCTCGTAACCAAACATGCACAGCGGTCAAACAGTATGTCCCAAG6580.33456549704331556No Hit
CCTAACTTAATGGACGGGAGGTATCCCAATAGGAGGTTTCCTCCTATGGT6430.32693862401041324No Hit
CCCTAACTTAATGGACGGGAGGTATCCCAATAGGAGGTTTCCTCCTATGG6080.3091425869336411No Hit
TGAAAATTCTATGGAAGAGCTAGCATCTCTGACGAAAACAGCAGACGGAA5790.29439729907003No Hit
CAGAAATCGCATTCATGAAATCAACACACAGCTTTCCACTGTCATTCGCC5590.2842281350261602No Hit
CGAAACTGGAAACCGACCGCCGCCATATCAGAAATCGCATTCATGAAATC5400.27456742918448385No Hit
CAGCAATTGAAGTGAAGATGATTGACCGCACGCAATTGATATTAGATATT5400.27456742918448385No Hit
CTAGCATCTCTGACGAAAACAGCAGACGGAAAAGTACTGACCAGCGTCAC5310.2699913053647425No Hit
CAAAAACGGAACAGGGCTGACGCCGCTACATATATAGGAAAAGGGAAGGT5300.269482847162549No Hit
CCCAATTATCCATAAAACTGTAACTAAGTGAGGCTCTCTCATTGGTTTAT5290.26897438896035547No Hit
CTCTCATTGGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCA5140.2613475159274532No Hit
CTTGAAGCTGATCTCCTCATCTTTAATGATGAACTGTCGCCAAGTCAGCT4970.25270372649016387No Hit
CATTAATTAGGGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAAT4550.23134848199803734No Hit
GGCCGATTGTCGCAGCCCGGGTCGATTATAACAACGGTGCAATCTCAGCT4450.22626389997610247No Hit
CTTCAAATTGCGCTTGTCGGCTATACAAACGCAGGGAAATCAACATGGTT4420.224738525369522No Hit
CTCATCTTTAATGATGAACTGTCGCCAAGTCAGCTGAAGTCATTGGCAAC4370.22219623435855454No Hit
GGAAGAGCTAGCATCTCTGACGAAAACAGCAGACGGAAAAGTACTGACCA4240.21558627773003922No Hit
CTTCCATTTCCGCCCAAGCTGCTCACAGTATACGGGCGTCGGCATCCAGA4030.20490865548397597No Hit
TATAGATTGGCGCTAGTAGATCACAGAACAACCGCCGCATACGGCCGATT3900.1982986988554606No Hit
CGCAGGGAAATCAACATGGTTCAACCGCCTGACGAGTGCTGACAGCTATG3890.19779024065326709No Hit
CTAACTTAATGGACGGGAGGTATCCCAATAGGAGGTTTCCTCCTATGGTT3890.19779024065326709No Hit
GAAAAAACAGTGCTTCGGCTGCTTGAGGAGCTTGAAGCAGATGATATCCC3880.1972817824510736No Hit
GTTTATTGGAAGCATCAACCTGCGCCGTCTTGTTAACTTGTCATATCGCG3860.19626486604668666No Hit
CCTCGATCCATCGCGTATAGGGACGCCCCCTGCTCGCGTTACTGCCAAGC3790.1927056586313322No Hit
GGCAAACTTCAAATTGAGCTGGCTCAGCTGCAATATGCACTGCCGCGTCT3710.18863799301378428No Hit
CTCTCTCATTGGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGC3600.1830449527896559No Hit
CTCAGCTAAACCGACGCAGTTTTGCTCCTTGGATTCTGAGCCCGGGCATC3550.18050266177868848No Hit
CAATTATCCATAAAACTGTAACTAAGTGAGGCTCTCTCATTGGTTTATAC3550.18050266177868848No Hit
GGAAAAGGGAAGGTAGAAGAGCTGAAGGCACTCGTGGAAGAGCTTGAAGC3530.1794857453743015No Hit
CGCGAATTGGGGACATTCAAATGAGCATCCTAGTCACCGCGTTTAAAATG3520.178977287172108No Hit
CGGAAAAGTACTGACCAGCGTCACACAAAAACGGAACAGGGCTGACGCCG3480.17694345436333406No Hit
CTGTCATTCGCCATAGAAGCCGATACCGTGAAAGAAGAAAGAAAAACGGT3480.17694345436333406No Hit
GAAAATTCTATGGAAGAGCTAGCATCTCTGACGAAAACAGCAGACGGAAA3430.17440116335236663No Hit
CATTGATTGCTGCATTCCGCTCAACGCTTGAGGAAGTAAAAGAAGCGGAT3430.17440116335236663No Hit
CATGGTTCAACCGCCTGACGAGTGCTGACAGCTATGAAGAAGACCTCCTG3390.17236733054359266No Hit
AGATGATTGACCGCACGCAATTGATATTAGATATTTTTGCAAAGCGGGCG3360.17084195593701218No Hit
GGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGGAGGTTTC3320.16880812312823826No Hit
ATTTATTCAGGATCTTCCGACGACATTGATTGCTGCATTCCGCTCAACGC3250.16524891571288383No Hit
GCTGAAGGCACTCGTGGAAGAGCTTGAAGCTGATCTCCTCATCTTTAATG3240.16474045751069033No Hit
ATTAGGGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGGAG3220.16372354110630336No Hit
CTGTAACTAAGTGAGGCTCTCTCATTGGTTTATACTTCAATATAAGCCTT3180.1616897082975294No Hit
CGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGGCTAGA3150.16016433369094893No Hit
CGAGAGAAGGCAAACTTCAAATTGAGCTGGCTCAGCTGCAATATGCACTG3130.15914741728656195No Hit
CGACGACATTGATTGCTGCATTCCGCTCAACGCTTGAGGAAGTAAAAGAA3100.1576220426799815No Hit
GCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGGAGGTTTCC3080.15660512627559453No Hit
CTTCACTGCCCCCTTTCAGCCTTTTGGGTCCTGTATCCCAATTCTCAGAG3060.15558820987120753No Hit
GGGATAGATAGCCACCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTT3050.15507975166901405No Hit
GTTGGTTTATGCTCATCCCTACTCATAACCCCAGCACTTAGATATTTTAA3010.1530459188602401No Hit
CTCTGACGAAAACAGCAGACGGAAAAGTACTGACCAGCGTCACACAAAAA3000.1525374606580466No Hit
GCAAACTTCAAATTGAGCTGGCTCAGCTGCAATATGCACTGCCGCGTCTG2950.14999516964707915No Hit
AAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTCTCTCA2930.14897825324269218No Hit
CTGAACTCGGATAGGTCTCAGAAAAACGAAATATAGGCTTACGGTAGGTC2910.1479613368383052No Hit
GAAAAACGGTGTGCTTCAAATTGCGCTTGTCGGCTATACAAACGCAGGGA2910.1479613368383052No Hit
CAACCTTTCCCGGCAAGGCGGAGGAATTGGGGCAAGAGGTCCCGGGGAAA2900.14745287863611173No Hit
ATCAGAAATCGCATTCATGAAATCAACACACAGCTTTCCACTGTCATTCG2900.14745287863611173No Hit
CCTGAAACGGGCATCGTCGCTCTTCGTTGTTCGTCGACTTCTAGTGTGGA2900.14745287863611173No Hit
GTAAAAGAAGCGGATTTAATTCTGCATTTAATTGATTCTTCAAATGAGGA2880.14643596223172473No Hit
GGAAGGTAGAAGAGCTGAAGGCACTCGTGGAAGAGCTTGAAGCTGATCTC2850.14491058762514428No Hit
CACAGAACAACCGCCGCATACGGCCGATTGTCGCAGCCCGGGTCGATTAT2840.1444021294229508No Hit
CTGAGGACCACCTGAAACGGGCATCGTCGCTCTTCGTTGTTCGTCGACTT2830.1438936712207573No Hit
CTTCAAATGAGGATTATGCGGGACATGAAAAAACAGTGCTTCGGCTGCTT2810.14287675481637033No Hit
GCTGCTCACAGTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGG2740.1393175474010159No Hit
TTATAAACTACTGCTAAGACAGCTAAGAAAGCTCCAATTGGTAGAGTTCC2720.13830063099662893No Hit
CTTCTTTCAGATACAGTAGGATTTATTCAGGATCTTCCGACGACATTGAT2670.13575833998566147No Hit
GGCTGACGCCGCTACATATATAGGAAAAGGGAAGGTAGAAGAGCTGAAGG2670.13575833998566147No Hit
GTGAGGCTCTCTCATTGGTTTATACTTCAATATAAGCCTTGGTAGGGATA2670.13575833998566147No Hit
GGGGAAACGAAACTGGAAACCGACCGCCGCCATATCAGAAATCGCATTCA2640.134232965379081No Hit
CCCGGATCGATGACGCGAATTGGGGACATTCAAATGAGCATCCTAGTCAC2640.134232965379081No Hit
GAGAAGGCAAACTTCAAATTGAGCTGGCTCAGCTGCAATATGCACTGCCG2630.13372450717688752No Hit
GATTTATTCAGGATCTTCCGACGACATTGATTGCTGCATTCCGCTCAACG2610.13270759077250055No Hit
CAAAAACTGCCTGATTTTATACCGACCGCCGGAAGGGATCACATTATGGT2600.13219913257030708No Hit
AAAAAAACGAACCTTTGGGTTCCAGAGCTGTACGGTCGCACTGAACTCGG2590.13169067436811357No Hit
ATTTTTGCAAAGCGGGCGAGAACGAGAGAAGGCAAACTTCAAATTGAGCT2570.1306737579637266No Hit
CGCCGCTACATATATAGGAAAAGGGAAGGTAGAAGAGCTGAAGGCACTCG2540.12914838335714612No Hit
CAACATGGTTCAACCGCCTGACGAGTGCTGACAGCTATGAAGAAGACCTC2530.12863992515495262No Hit
AGAAGACCTCCTGTTTGCCACGCTGGACCCGATGACCAGAAAAATGGTCC2500.12711455054837217No Hit
ATTAATTAGGGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATA2480.1260976341439852No Hit
CGGGACATGAAAAAACAGTGCTTCGGCTGCTTGAGGAGCTTGAAGCAGAT2460.1250807177395982No Hit
GTTAACTTGTCATATCGCGCACGTAGTAGCCTAGAGCGCCAGGGGCGGAA2460.1250807177395982No Hit
CAGGAACATCCTCGTGTTAGATATTGAGGCTGCTTCGTGTCGGCACGAAG2450.12457225953740472No Hit
CTATGGAAGAGCTAGCATCTCTGACGAAAACAGCAGACGGAAAAGTACTG2430.12355534313301775No Hit
GTCAAACAGTATGTCCCAAGGGGACTTAAGCGCGGTGGCCTCCCCTATCC2420.12304688493082426No Hit
CGCTGGACCCGATGACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTT2410.12253842672863079No Hit
AGCGGATTTAATTCTGCATTTAATTGATTCTTCAAATGAGGATTATGCGG2400.12202996852643729No Hit
CTTATACAGAATTATCAATCAAGCTCCCCGAGGAGCGGACTTGTAAGGAC2400.12202996852643729No Hit
CAGGATCTTCCGACGACATTGATTGCTGCATTCCGCTCAACGCTTGAGGA2390.1215215103242438No Hit
GAAAGATCATGTTTATTGGAAGCATCAACCTGCGCCGTCTTGTTAACTTG2380.1210130521220503No Hit
GAACAACCGCCGCATACGGCCGATTGTCGCAGCCCGGGTCGATTATAACA2350.11948767751546983No Hit
CAAACATGCACAGCGGTCAAACAGTATGTCCCAAGGGGACTTAAGCGCGG2350.11948767751546983No Hit
CTATCACATAAGGCATCATTATAACTAAAAATGGGATATATTCCTTATAA2340.11897921931327635No Hit
CGTAACCAAACATGCACAGCGGTCAAACAGTATGTCCCAAGGGGACTTAA2340.11897921931327635No Hit
CAGCGGTCAAACAGTATGTCCCAAGGGGACTTAAGCGCGGTGGCCTCCCC2320.11796230290888937No Hit
CGCCTGACGAGTGCTGACAGCTATGAAGAAGACCTCCTGTTTGCCACGCT2320.11796230290888937No Hit
GGATAGATAGCCACCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTG2320.11796230290888937No Hit
AGAACAACCGCCGCATACGGCCGATTGTCGCAGCCCGGGTCGATTATAAC2310.11745384470669588No Hit
CTAGCCTATCGCAGACGGTATCAACAGGAACATCCTCGTGTTAGATATTG2310.11745384470669588No Hit
ATTCCTTATAAACTACTGCTAAGACAGCTAAGAAAGCTCCAATTGGTAGA2300.11694538650450238No Hit
CTCATCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTA2290.11643692830230891No Hit
GAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTCTCTCATTG2290.11643692830230891No Hit
CACAGCTTTCCACTGTCATTCGCCATAGAAGCCGATACCGTGAAAGAAGA2250.11440309549353496No Hit
CTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGGAGGTTTCCT2240.11389463729134146No Hit
CGAGTGCTGACAGCTATGAAGAAGACCTCCTGTTTGCCACGCTGGACCCG2240.11389463729134146No Hit
CTAGCGTACGAGCACTATGGTCAGTAATTCCTGGAGGAATAGGTACCAAG2210.112369262684761No Hit
CTCCCCTATCCCCTACGAGGCTACCCGGATCGATGACGCGAATTGGGGAC2210.112369262684761No Hit
TTATAACTAAAAATGGGATATATTCCTTATAAACTACTGCTAAGACAGCT2200.11186080448256752No Hit
AGAGAAGGCAAACTTCAAATTGAGCTGGCTCAGCTGCAATATGCACTGCC2180.11084388807818053No Hit
CCTTGACCGCGGGAAGGAGACATAACGATAGCGACTCTGTCTCAGGGGAT2150.10931851347160007No Hit
CCCAATTCTCAGAGGTCCCGCCGTACGCTGAGGACCACCTGAAACGGGCA2130.1083015970672131No Hit
ATTTAATTCTGCATTTAATTGATTCTTCAAATGAGGATTATGCGGGACAT2110.1072846806628261No Hit
CTGTCGCCAAGTCAGCTGAAGTCATTGGCAACAGCAATTGAAGTGAAGAT2090.10626776425843915No Hit
CTTTTGGGTCCTGTATCCCAATTCTCAGAGGTCCCGCCGTACGCTGAGGA2090.10626776425843915No Hit
CATCATTATAACTAAAAATGGGATATATTCCTTATAAACTACTGCTAAGA2090.10626776425843915No Hit
CTTTTCCCCACTTCCAAGCATTTTTTCAACTAATCTTATGTTATTAACCA2080.10575930605624564No Hit
GGGAAACGAAACTGGAAACCGACCGCCGCCATATCAGAAATCGCATTCAT2080.10575930605624564No Hit
CGTGGTTTTACTAGGCTAGACTAGCGTACGAGCACTATGGTCAGTAATTC2070.10525084785405216No Hit
GGGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGGAGGTTT2070.10525084785405216No Hit
CTCGTGTTAGATATTGAGGCTGCTTCGTGTCGGCACGAAGTGTCTTCCGA2060.10474238965185866No Hit
GGGAGGTATCCCAATAGGAGGTTTCCTCCTATGGTTTTCAAAACAATCAC2050.10423393144966518No Hit
ATTACTTCCATTTCCGCCCAAGCTGCTCACAGTATACGGGCGTCGGCATC2050.10423393144966518No Hit
CGATGACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCA2050.10423393144966518No Hit
GCTCGAGTTGCCCCTTTGCCCGATCCCGGAAGATACGCTCTAAGCTCGGC2050.10423393144966518No Hit
CTTGGCTTCCTTCCGCAGTCAAAACCGCGCAATTATCCCCGTCCTGATTT2040.1037254732474717No Hit
CTGAAACGGGCATCGTCGCTCTTCGTTGTTCGTCGACTTCTAGTGTGGAG2030.1032170150452782No Hit
GAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGGAGGTTTCCTCC2030.1032170150452782No Hit
GAGGAATTGGGGCAAGAGGTCCCGGGGAAACGAAACTGGAAACCGACCGC2010.10220009864089123No Hit
CAATAACGTCTCTTTCAGAAGGCATTGGTATCTTTTCCCCACTTCCAAGC2000.10169164043869773No Hit
CCGATACCGTGAAAGAAGAAAGAAAAACGGTGTGCTTCAAATTGCGCTTG1990.10118318223650426No Hit
CCGCGTTTAAAATGAACCTGCCGGCTGATCGTTTTTAGGATATTGTGAGT1990.10118318223650426No Hit
GACGAATTGCCAGAATTATTAACTGCGCAGTTAGGGCAGCGTCTGAGGAA1980.10067472403431076No Hit
AATCAACACACAGCTTTCCACTGTCATTCGCCATAGAAGCCGATACCGTG1980.10067472403431076No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)