FastQCFastQC Report
Fri 28 Oct 2022
SRR1553606_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1553606_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10000
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGT6846.84No Hit
ATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTT4114.109999999999999RNA PCR Primer, Index 39 (95% over 23bp)
ACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCT3273.27RNA PCR Primer, Index 39 (96% over 25bp)
CATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTGCT1071.0699999999999998RNA PCR Primer, Index 39 (96% over 28bp)
TTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTC690.69No Hit
CACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTG630.63Illumina PCR Primer Index 11 (96% over 25bp)
TATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCT600.6RNA PCR Primer, Index 39 (95% over 22bp)
ACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTGC210.21Illumina PCR Primer Index 11 (96% over 26bp)
CTTTACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTC180.18RNA PCR Primer, Index 39 (95% over 21bp)
TCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTGCTTG110.11RNA PCR Primer, Index 39 (96% over 30bp)
CTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTC110.11No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)