FastQCFastQC Report
Thu 3 Nov 2022
hcc1395_normal_rep1_trimmed_r1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenamehcc1395_normal_rep1_trimmed_r1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences223357
Sequences flagged as poor quality0
Sequence length50-151
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGAAAATAACTTTTATTGAGACCCCACCAACTGCAAAATCTGTTCCTG4630.20729146612821625No Hit
GCCAATTCTTATAGAAACGCCTCTTGCATTCATCACTGATGTGCTCAGCA3100.13879126241846013No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT2910.13028470117345772No Hit
GGTCACTTCACCATAGTGGACAAAGCCACCCAGAGGGTTGATGCTCTTGT2600.11640557493161172No Hit
GTTGTCAATGAGGATATTTATTGGGGTTTCATGAGTGCAGGGAGAAGGGC2410.10789901368660933No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)