FastQCFastQC Report
Wed 19 Feb 2025
hcc1395_normal_rep1_R1.fq

Summary

[OK]Basic Statistics

MeasureValue
Filenamehcc1395_normal_rep1_R1.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences331958
Total Bases50.1 Mbp
Sequences flagged as poor quality0
Sequence length151
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGAAAATAACTTTTATTGAGACCCCACCAACTGCAAAATCTGTTCCTG6430.19369920291121165No Hit
GCCAATTCTTATAGAAACGCCTCTTGCATTCATCACTGATGTGCTCAGCA4630.13947547581320527No Hit
GGTCACTTCACCATAGTGGACAAAGCCACCCAGAGGGTTGATGCTCTTGT3780.11386982690581339No Hit
GTTGTCAATGAGGATATTTATTGGGGTTTCATGAGTGCAGGGAGAAGGGC3540.1066399966260792No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT3350.10091638098795631No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)