FastQCFastQC Report
Wed 19 Feb 2025
hcc1395_normal_rep2_R1.fq

Summary

[OK]Basic Statistics

MeasureValue
Filenamehcc1395_normal_rep2_R1.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences331958
Total Bases50.1 Mbp
Sequences flagged as poor quality0
Sequence length151
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGAAAATAACTTTTATTGAGACCCCACCAACTGCAAAATCTGTTCCTG6910.20815886347068002No Hit
GCCAATTCTTATAGAAACGCCTCTTGCATTCATCACTGATGTGCTCAGCA4350.13104067382018206No Hit
GTTGTCAATGAGGATATTTATTGGGGTTTCATGAGTGCAGGGAGAAGGGC3570.10754372541104597No Hit
GGTCACTTCACCATAGTGGACAAAGCCACCCAGAGGGTTGATGCTCTTGT3460.10423005319950113No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT3360.10121762391627857No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)