FastQCFastQC Report
Wed 19 Feb 2025
hcc1395_normal_rep3_R1.fq

Summary

[OK]Basic Statistics

MeasureValue
Filenamehcc1395_normal_rep3_R1.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences331956
Total Bases50.1 Mbp
Sequences flagged as poor quality0
Sequence length151
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGAAAATAACTTTTATTGAGACCCCACCAACTGCAAAATCTGTTCCTG6820.20544891491643472No Hit
GCCAATTCTTATAGAAACGCCTCTTGCATTCATCACTGATGTGCTCAGCA4340.13074021858318574No Hit
GTTGTCAATGAGGATATTTATTGGGGTTTCATGAGTGCAGGGAGAAGGGC3740.11266553398643195No Hit
GGTCACTTCACCATAGTGGACAAAGCCACCCAGAGGGTTGATGCTCTTGT3500.10543566014773041No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT3330.10031449951198351No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)