FastQCFastQC Report
Wed 19 Feb 2025
hcc1395_tumor_rep3_R1.fq

Summary

[OK]Basic Statistics

MeasureValue
Filenamehcc1395_tumor_rep3_R1.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences390607
Total Bases58.9 Mbp
Sequences flagged as poor quality0
Sequence length151
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT8010.20506544941590907No Hit
CTACGTTCTCCTACAAATGGACCTTGAGAGCTTGTTTGGAGGTTCTAGCA6690.171271892208793No Hit
GGGAGCAGAGGCAGCTGCCTTTATTGGGGGCCATGGGCTGGCTGATTTCA6570.1681997506445097No Hit
GTGGAAAATAACTTTTATTGAGACCCCACCAACTGCAAAATCTGTTCCTG6280.1607754085308251No Hit
GGGAACTTGAATTCGTATCCATCTGGCAGCTTGACGGTCAGGTTGGCCTG4580.11725340303681193No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)