FastQCFastQC Report
Tue 21 Jul 2026
SRR12904817_R1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR12904817_R1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2000000
Total Bases261.5 Mbp
Sequences flagged as poor quality0
Sequence length35-150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN514432.57215No Hit
CGGGAACGTATTCACCGCGGCGTGCTGATCCGCGATTACTAGCGATTCCG29830.14915No Hit
GCGAAATTCCTTGTCGGGTAAGTTCCGACCTGCACGAATGGCATAATGAT21810.10905000000000001No Hit
CCGGGAACGTATTCACCGCGGCGTGCTGATCCGCGATTACTAGCGATTCC20770.10385No Hit
CTCAGTTCCAGTGTGGCTGGTCATCCTCTCAGACCAGCTAGAGATCGCAG20530.10265No Hit
GGAAACGATAGCTAATACCGCATAAGAGTGGATGTTGCATGACATTTGCT20460.1023No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGAGCT3050.039.6999743
TACGAAG4250.029.9900729
TGAACGC5450.029.2334448
TTTGATC8450.027.9049642
CCTCGAG4400.027.520011
GCGAAAT18550.026.7977491
CCCGAAT1501.0584826E-425.4922161
CGAAATT19050.025.4246582
CCGGGAA25250.024.9874211
TGGTTCG7700.024.8294453
GCACGGA4400.024.6225344
CAGATTG7950.024.0486473
CGAGCTA5050.023.9772134
CGCGTAT1652.02079E-423.1747441
TTGATCA6950.022.9240683
GATTGAA8100.022.8165235
AGTTCCA20450.022.4375634
CGAACGC5450.022.2179871
TTTACTC7500.022.0926882
GGAACGT29600.021.9606383
Produced by FastQC (version 0.12.1)