##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	SRR12904817_R2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2000000
Total Bases	266.4 Mbp
Sequences flagged as poor quality	0
Sequence length	35-150
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	35.369305	37.0	37.0	37.0	37.0	37.0
2	35.4569935	37.0	37.0	37.0	37.0	37.0
3	35.719414	37.0	37.0	37.0	37.0	37.0
4	35.696558	37.0	37.0	37.0	37.0	37.0
5	35.7438505	37.0	37.0	37.0	37.0	37.0
6	35.785314	37.0	37.0	37.0	37.0	37.0
7	35.67428	37.0	37.0	37.0	37.0	37.0
8	35.667948	37.0	37.0	37.0	37.0	37.0
9	35.742299	37.0	37.0	37.0	37.0	37.0
10-14	35.6933088	37.0	37.0	37.0	37.0	37.0
15-19	35.707984	37.0	37.0	37.0	37.0	37.0
20-24	35.6644085	37.0	37.0	37.0	37.0	37.0
25-29	35.47342880000001	37.0	37.0	37.0	37.0	37.0
30-34	35.4632518	37.0	37.0	37.0	37.0	37.0
35-39	35.5697049755981	37.0	37.0	37.0	37.0	37.0
40-44	35.60164701304205	37.0	37.0	37.0	37.0	37.0
45-49	35.556748544990846	37.0	37.0	37.0	37.0	37.0
50-54	35.54102074049921	37.0	37.0	37.0	37.0	37.0
55-59	35.56765668750869	37.0	37.0	37.0	37.0	37.0
60-64	35.60102775259857	37.0	37.0	37.0	37.0	37.0
65-69	35.53655410788572	37.0	37.0	37.0	37.0	37.0
70-74	35.48326741517155	37.0	37.0	37.0	37.0	37.0
75-79	35.46058316551142	37.0	37.0	37.0	37.0	37.0
80-84	35.506783793262024	37.0	37.0	37.0	37.0	37.0
85-89	35.61592369531446	37.0	37.0	37.0	37.0	37.0
90-94	35.66368489149609	37.0	37.0	37.0	37.0	37.0
95-99	35.66632383225358	37.0	37.0	37.0	37.0	37.0
100-104	35.657123617722775	37.0	37.0	37.0	37.0	37.0
105-109	35.64955480742626	37.0	37.0	37.0	37.0	37.0
110-114	35.65346927622012	37.0	37.0	37.0	37.0	37.0
115-119	35.60024769966043	37.0	37.0	37.0	37.0	37.0
120-124	35.53218769364865	37.0	37.0	37.0	37.0	37.0
125-129	35.5266596823251	37.0	37.0	37.0	37.0	37.0
130-134	35.45909511782156	37.0	37.0	37.0	37.0	37.0
135-139	35.464924421918965	37.0	37.0	37.0	37.0	37.0
140-144	35.36175221131604	37.0	37.0	37.0	37.0	37.0
145-149	35.25353724184893	37.0	37.0	37.0	32.2	37.0
150	35.39879835608436	37.0	37.0	37.0	37.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	9096.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	1.0
12	46.0
13	282.0
14	599.0
15	1047.0
16	1513.0
17	1874.0
18	2208.0
19	2552.0
20	3038.0
21	3540.0
22	4398.0
23	5233.0
24	6553.0
25	8564.0
26	9939.0
27	10602.0
28	11987.0
29	14503.0
30	19018.0
31	26285.0
32	37010.0
33	55898.0
34	92853.0
35	225719.0
36	1174315.0
37	271327.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.580413721605865	9.391110771790101	15.11278293679655	36.91569256980748
2	31.399104999555465	14.984300573075545	30.167462228198495	23.4491321991705
3	32.615284313055774	20.415399235724074	21.185752803751463	25.78356364746869
4	33.09175128484347	28.28604493235234	17.306710921269936	21.315492861534256
5	31.928476676161523	29.257126037782854	19.916289241615488	18.898108044440136
6	26.026568835061862	32.16267584976473	20.805071465022923	21.005683850150483
7	21.931795807331746	16.228105423465923	38.45102526289565	23.389073506306683
8	24.601286651691893	19.96655790535355	26.89205506644218	28.540100376512378
9	25.970061841254022	19.785685296729525	28.210551588625066	26.033701273391387
10-14	26.90173910946987	24.726556378409004	24.204783354697163	24.166921157423964
15-19	27.28349533679173	24.071225935554903	24.79411362878371	23.851165098869657
20-24	27.44130144477392	24.218882088136663	24.438184497519714	23.901631969569706
25-29	26.2954874679573	25.01976765892444	24.382440250429866	24.30230462268839
30-34	25.82770643020476	24.764801545400356	25.021051899614893	24.386440124779984
35-39	25.79875625123311	25.23988040730345	24.755923375362848	24.205439966100595
40-44	26.313265488215908	25.100669319144338	24.571437188809327	24.014628003830428
45-49	25.215424924450286	24.79151980565618	25.39974942649403	24.593305843399506
50-54	25.4698517043846	24.872277207096054	25.501916382846623	24.155954705672723
55-59	25.88000778936007	24.260028423306384	25.228983713165377	24.630980074168164
60-64	26.477504918490702	24.221497982147312	25.189282170804226	24.111714928557763
65-69	26.117395051318677	24.77754285088739	24.793103469208493	24.311958628585444
70-74	25.661640890751087	25.385403945083514	24.799148676779197	24.153806487386202
75-79	25.61029512372817	25.354949479919124	24.71943550713655	24.31531988921616
80-84	25.944773005764294	25.396380689340088	24.60402279895068	24.054823505944935
85-89	26.718442621873827	25.10758217991943	24.42669175402129	23.747283444185456
90-94	26.66815512037028	24.94711082755519	24.275952870025975	24.10878118204856
95-99	26.398410849028448	24.998640334101967	24.385707368947	24.217241447922586
100-104	27.19463725436136	24.911207782369317	24.009122781166965	23.885032182102353
105-109	27.069503388176724	24.642320134734874	24.225145411060197	24.063031066028202
110-114	26.81127690641348	24.8601886041263	23.93457842489799	24.39395606456223
115-119	27.14804852041088	24.50390676233071	23.95191564920399	24.396129068054417
120-124	27.22595650899125	24.587940022620725	23.715950624425428	24.470152843962595
125-129	27.125956002409218	24.339207986759973	23.739574279562305	24.795261731268504
130-134	27.11811455285219	24.57568877173752	23.646749866113815	24.65944680929647
135-139	27.309752340611826	24.49247232096773	23.943196898946795	24.254578439473647
140-144	27.32180054482951	24.28928762251445	23.184899785099393	25.204012047556652
145-149	27.877511074004502	23.162429555416406	24.159521324706045	24.80053804587305
150	30.540809301614015	0.0	34.11778613798043	35.34140456040555
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	9162.0
1	4605.0
2	59.0
3	92.0
4	115.0
5	130.0
6	149.5
7	162.0
8	180.5
9	207.0
10	209.5
11	189.5
12	200.0
13	196.5
14	201.0
15	238.0
16	239.5
17	258.0
18	322.5
19	429.5
20	603.0
21	787.0
22	1047.0
23	1396.0
24	1828.0
25	2337.5
26	2929.5
27	3750.5
28	4704.5
29	5629.0
30	6565.0
31	7683.0
32	8850.0
33	10021.0
34	11219.5
35	12393.5
36	14207.0
37	16145.5
38	17866.0
39	20941.0
40	25235.5
41	31885.5
42	39265.0
43	44311.0
44	49029.5
45	60855.0
46	73879.5
47	87977.0
48	109048.0
49	124880.0
50	124537.5
51	124720.5
52	130137.5
53	133517.5
54	131427.5
55	118982.5
56	101231.0
57	73362.5
58	49060.0
59	36299.0
60	27955.5
61	24306.5
62	22549.0
63	19207.5
64	14077.5
65	10321.0
66	8495.5
67	7060.0
68	6132.5
69	5284.0
70	4488.0
71	3871.0
72	3362.5
73	2961.5
74	2686.5
75	2469.0
76	2232.5
77	2055.5
78	1931.0
79	1657.0
80	1445.0
81	1294.0
82	1155.0
83	1070.5
84	974.0
85	935.0
86	918.0
87	927.0
88	992.5
89	1057.5
90	1090.5
91	1160.0
92	1271.5
93	1400.0
94	1503.5
95	1681.5
96	1968.0
97	2139.0
98	2333.0
99	2584.0
100	3958.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.4548
2	0.45814999999999995
3	0.4548
4	0.4548
5	0.45485
6	0.4548
7	0.4548
8	0.4548
9	0.4548
10-14	0.4548
15-19	0.4548
20-24	0.45709
25-29	0.46874000000000005
30-34	0.48403999999999997
35-39	0.09954463900308363
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	1.0296065655951474E-4
105-109	1.1894807084071307E-5
110-114	0.0
115-119	0.0
120-124	0.0
125-129	3.032455066958052E-4
130-134	3.052405684861396E-5
135-139	0.0
140-144	0.0
145-149	0.0
150	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
35-39	10424.0
40-44	642.0
45-49	1082.0
50-54	1618.0
55-59	2770.0
60-64	4177.0
65-69	7107.0
70-74	11632.0
75-79	17386.0
80-84	26742.0
85-89	36613.0
90-94	48383.0
95-99	57126.0
100-104	65491.0
105-109	69157.0
110-114	73683.0
115-119	75675.0
120-124	74745.0
125-129	75685.0
130-134	73785.0
135-139	69468.0
140-144	66152.0
145-149	411442.0
150-151	719015.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	37.30595472014953
#Duplication Level	Percentage of total
1	33.28333978941489
2	3.301569748009127
3	1.6319579748400186
4	1.2340624225101635
5	0.9780579696415894
6	0.8666926970749581
7	0.7166456253037299
8	0.6862019814418273
9	0.6257595820819749
>10	14.23643072488964
>50	9.548885590553436
>100	24.85351620091907
>500	5.35380408666904
>1k	1.9301508239368912
>5k	0.7529247827135982
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN	9096	0.4548	No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG	5966	0.2983	No Hit
CGGGAACGTATTCACCGCGGCGTGCTGATCCGCGATTACTAGCGATTCCG	2735	0.13675	No Hit
GCGAAATTCCTTGTCGGGTAAGTTCCGACCTGCACGAATGGCATAATGAT	2023	0.10115	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	1.5E-4	0.0	0.0	0.0	0.00635	1.9509
2	1.5E-4	0.0	0.0	0.0	0.0095	1.954
3	1.5E-4	0.0	0.0	0.0	0.01195	1.9547
4	1.5E-4	0.0	0.0	0.0	0.0164	1.95515
5	1.5E-4	0.0	0.0	0.0	0.0201	1.95565
6	1.5E-4	0.0	0.0	0.0	0.02455	1.9566
7	1.5E-4	0.0	0.0	0.0	0.03125	1.95765
8	1.5E-4	0.0	0.0	0.0	0.04015	1.9594
9	1.5E-4	0.0	0.0	0.0	0.0502	1.96455
10-11	1.5E-4	0.0	0.0	0.0	0.063525	1.9711750000000001
12-13	1.5E-4	0.0	0.0	0.0	0.07375000000000001	1.9888249999999998
14-15	1.5E-4	0.0	0.0	0.0	0.086675	2.000225
16-17	2.0E-4	0.0	0.0	0.0	0.1033	2.00195
18-19	2.0E-4	0.0	0.0	0.0	0.11835000000000001	2.00345
20-21	2.0E-4	0.0	0.0	0.0	0.136375	2.0044750000000002
22-23	2.25E-4	0.0	0.0	0.0	0.15455	2.005725
24-25	3.0E-4	0.0	0.0	0.0	0.1899	2.006925
26-27	3.5E-4	0.0	0.0	0.0	0.209925	2.00825
28-29	3.5E-4	0.0	0.0	0.0	0.221925	2.009225
30-31	3.75E-4	0.0	0.0	0.0	0.23320000000000002	2.0098000000000003
32-33	4.0E-4	0.0	0.0	0.0	0.24509999999999998	2.0108249999999996
34-35	4.0E-4	0.0	0.0	0.0	0.262925	2.0116
36-37	4.0E-4	0.0	0.0	0.0	0.27497499999999997	2.0123249999999997
38-39	4.75E-4	0.0	0.0	0.0	0.28415	2.012925
40-41	5.5E-4	0.0	0.0	0.0	0.29325	2.01375
42-43	6.000000000000001E-4	0.0	0.0	0.0	0.300275	2.0143
44-45	6.5E-4	0.0	0.0	0.0	0.31372500000000003	2.0147
46-47	7.5E-4	0.0	0.0	0.0	0.324625	2.0153
48-49	8.749999999999999E-4	0.0	0.0	0.0	0.33240000000000003	2.01605
50-51	9.25E-4	0.0	0.0	0.0	0.33895	2.016775
52-53	0.0010999999999999998	0.0	0.0	0.0	0.34597500000000003	2.01785
54-55	0.0012499999999999998	0.0	0.0	0.0	0.35702500000000004	2.01885
56-57	0.00135	0.0	0.0	0.0	0.365625	2.0203249999999997
58-59	0.001475	0.0	0.0	0.0	0.37365	2.0215
60-61	0.00155	0.0	0.0	0.0	0.382525	2.0223
62-63	0.001575	0.0	0.0	0.0	0.39397499999999996	2.022925
64-65	0.00165	0.0	0.0	0.0	0.417625	2.0233499999999998
66-67	0.001725	0.0	0.0	0.0	0.460425	2.023875
68-69	0.0019	0.0	0.0	5.0E-5	0.503475	2.0244
70-71	0.002275	0.0	0.0	5.0E-5	0.5561	2.0248999999999997
72-73	0.002375	0.0	0.0	5.0E-5	0.5729	2.025475
74-75	0.0025250000000000003	0.0	0.0	5.0E-5	0.5805750000000001	2.02645
76-77	0.0026750000000000003	0.0	0.0	5.0E-5	0.588425	2.027875
78-79	0.00295	0.0	0.0	5.0E-5	0.59615	2.0301
80-81	0.003325	0.0	0.0	5.0E-5	0.60215	2.033225
82-83	0.003725	0.0	0.0	7.500000000000001E-5	0.60885	2.0374499999999998
84-85	0.0043	0.0	0.0	1.0E-4	0.618125	2.0424499999999997
86-87	0.00465	0.0	0.0	1.0E-4	0.627125	2.046825
88-89	0.0055	0.0	0.0	1.0E-4	0.6331249999999999	2.05375
90-91	0.00655	0.0	0.0	1.0E-4	0.639625	2.060425
92-93	0.0074	0.0	0.0	1.0E-4	0.6456999999999999	2.0673
94-95	0.008324999999999999	0.0	0.0	1.0E-4	0.655725	2.0749
96-97	0.00925	0.0	0.0	1.0E-4	0.663425	2.083675
98-99	0.010499999999999999	0.0	0.0	1.0E-4	0.6683749999999999	2.091375
100-101	0.0118	0.0	0.0	1.0E-4	0.673125	2.0976999999999997
102-103	0.0132	0.0	0.0	1.0E-4	0.6794	2.103275
104-105	0.01485	0.0	0.0	1.0E-4	0.684675	2.1094
106-107	0.016025	0.0	0.0	1.0E-4	0.689875	2.115475
108-109	0.0174	5.0E-5	0.0	1.0E-4	0.6957249999999999	2.12245
110-111	0.01875	5.0E-5	0.0	1.0E-4	0.700225	2.129025
112-113	0.020175	5.0E-5	0.0	1.0E-4	0.704475	2.134975
114-115	0.02145	5.0E-5	0.0	1.25E-4	0.708975	2.140125
116-117	0.02305	5.0E-5	0.0	1.5E-4	0.7147	2.1448
118-119	0.024675	5.0E-5	0.0	1.5E-4	0.719025	2.1487249999999998
120-121	0.026425	5.0E-5	0.0	1.5E-4	0.72285	2.153375
122-123	0.028525000000000002	5.0E-5	0.0	1.5E-4	0.726075	2.1586499999999997
124-125	0.031200000000000002	5.0E-5	0.0	1.5E-4	0.729825	2.163725
126-127	0.033375	5.0E-5	0.0	1.5E-4	0.7348	2.168575
128-129	0.0349	5.0E-5	0.0	1.5E-4	0.739375	2.1734999999999998
130-131	0.035975	5.0E-5	0.0	1.75E-4	0.744075	2.1784499999999998
132-133	0.040625	5.0E-5	0.0	2.0E-4	0.749825	2.1847250000000003
134-135	0.04517500000000001	5.0E-5	0.0	2.0E-4	0.75515	2.190575
136-137	0.046875	5.0E-5	0.0	2.0E-4	0.75995	2.196325
138-139	0.0469	5.0E-5	0.0	2.0E-4	0.7626	2.202025
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGAGCT	440	0.0	39.216797	3
CCTCGAG	465	0.0	38.484684	1
TTTACTC	710	0.0	36.006798	2
TGAACGC	550	0.0	33.6974	8
CGAGCTA	540	0.0	33.137928	4
CGGAATT	540	0.0	27.22181	1
CTCTTCG	915	0.0	26.541386	6
TTGATCA	710	0.0	26.103617	3
GATTGAA	895	0.0	24.992292	5
TACGAAG	435	0.0	24.975876	9
TTTGATC	955	0.0	24.761744	2
CAGATTG	835	0.0	24.492018	3
GCGAAAT	1715	0.0	24.223232	1
TGGTTCG	805	0.0	23.816967	3
ACGCTCA	1075	0.0	23.78004	3
GCCGTAT	135	0.0016864003	23.671139	1
AACGTAT	3200	0.0	23.566391	5
CGAAATT	1655	0.0	23.556715	2
CGCTCAT	1060	0.0	23.513636	4
CGTATTC	3250	0.0	23.400473	7
>>END_MODULE
