FastQCFastQC Report
Tue 21 Jul 2026
SRR12904818_R1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR12904818_R1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2000000
Total Bases271.7 Mbp
Sequences flagged as poor quality0
Sequence length35-150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN215031.07515No Hit
GGCCAATCAAACTCCGTGATAGCTGGTTCTCCCCGAAATGCATTTAGGTG33710.16855No Hit
CCGGGAACGTATTCACCGCAGCATTGCTGATCTGCGATTACTAGCGACTC31760.1588No Hit
CGGGAACGTATTCACCGCAGCATTGCTGATCTGCGATTACTAGCGACTCC30060.1503No Hit
CGGCGAATTAGTACCAGTCACCTCCACGCCTTACAACGCTTCCAGATCTA28530.14265No Hit
CGCGCCTCCACAACCTCTTACAGCTGCTTCACACTGGCCATGGGTAGATC27080.1354No Hit
CGTCAAGTCATCATGCCCCTTATGTCCAGGGCTTCACACATGCTACAATG26860.1343No Hit
CGGCCTTCGGGTTGTAAACTCCTTTCGCCAGGGACGAAGCGTTTTGTGAC25620.1281No Hit
GTCAAGTCATCATGCCCCTTATGTCCAGGGCTTCACACATGCTACAATGG23740.11869999999999999No Hit
GGGTGATCTGCCCTGCACTCTGGGATAAGCCTGGGAAACTGGGTCTAATA21980.1099No Hit
CGGCGATCTGGGCTGTTTCCCTCTCGACTATGAAGCTTATCCCCCACAGT21880.1094No Hit
CGCCGGATGACTAAGGGTTCCTGGGTCAAGTTCGTCTTCCCAGGGTGAGT21680.1084No Hit
GCCGAATATGCACTCGCTAGAGGCTTTTCTCGACAGCACAAGCACACCAC20140.10070000000000001No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGCGAA8350.041.516291
CTGGCTC6950.031.9972179
GGATTCA7850.031.6615419
GCGGGTT1252.5598385E-531.395711
CGGATTC8200.030.3101378
GGAACGG8200.029.5125034
GGTTCGC8150.028.8910314
ATGTACT2053.1042873E-828.7148676
TCACGAG2008.0084465E-726.1630942
CGCGCCT13000.026.1630941
TCCGATC8750.026.1630922
TGATCCT9000.026.1624344
GTTCGCG9800.024.6941325
GATCCTG10450.023.784035
TTTGATC12600.023.3599052
ATTAGTA18600.023.2086097
CTCGCCA1702.0745717E-423.0850811
GCTACAT1702.0745717E-423.0850813
CGATCAA9950.023.0071644
CCGGGAA34950.022.8318841
Produced by FastQC (version 0.12.1)