##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	SRR12904818_R2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2000000
Total Bases	273.6 Mbp
Sequences flagged as poor quality	0
Sequence length	35-150
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	35.3971995	37.0	37.0	37.0	37.0	37.0
2	35.45464	37.0	37.0	37.0	37.0	37.0
3	35.7648575	37.0	37.0	37.0	37.0	37.0
4	35.7757295	37.0	37.0	37.0	37.0	37.0
5	35.7815725	37.0	37.0	37.0	37.0	37.0
6	35.8269535	37.0	37.0	37.0	37.0	37.0
7	35.7782325	37.0	37.0	37.0	37.0	37.0
8	35.7982385	37.0	37.0	37.0	37.0	37.0
9	35.8920195	37.0	37.0	37.0	37.0	37.0
10-14	35.873177899999995	37.0	37.0	37.0	37.0	37.0
15-19	35.88836310000001	37.0	37.0	37.0	37.0	37.0
20-24	35.8583343	37.0	37.0	37.0	37.0	37.0
25-29	35.749595799999994	37.0	37.0	37.0	37.0	37.0
30-34	35.736677900000004	37.0	37.0	37.0	37.0	37.0
35-39	35.78721838907438	37.0	37.0	37.0	37.0	37.0
40-44	35.805501317678186	37.0	37.0	37.0	37.0	37.0
45-49	35.76820081287939	37.0	37.0	37.0	37.0	37.0
50-54	35.76278920292849	37.0	37.0	37.0	37.0	37.0
55-59	35.76518188290943	37.0	37.0	37.0	37.0	37.0
60-64	35.784780741018906	37.0	37.0	37.0	37.0	37.0
65-69	35.73373651324015	37.0	37.0	37.0	37.0	37.0
70-74	35.69355935832273	37.0	37.0	37.0	37.0	37.0
75-79	35.67360848260946	37.0	37.0	37.0	37.0	37.0
80-84	35.703458491210334	37.0	37.0	37.0	37.0	37.0
85-89	35.7651121974126	37.0	37.0	37.0	37.0	37.0
90-94	35.75622741976271	37.0	37.0	37.0	37.0	37.0
95-99	35.73394729409824	37.0	37.0	37.0	37.0	37.0
100-104	35.70376518810913	37.0	37.0	37.0	37.0	37.0
105-109	35.67365617654383	37.0	37.0	37.0	37.0	37.0
110-114	35.67376222062619	37.0	37.0	37.0	37.0	37.0
115-119	35.636747835667904	37.0	37.0	37.0	37.0	37.0
120-124	35.55467124302199	37.0	37.0	37.0	37.0	37.0
125-129	35.559803695704105	37.0	37.0	37.0	37.0	37.0
130-134	35.49243919824681	37.0	37.0	37.0	37.0	37.0
135-139	35.50303357822791	37.0	37.0	37.0	37.0	37.0
140-144	35.39828447810224	37.0	37.0	37.0	37.0	37.0
145-149	35.308266105116935	37.0	37.0	37.0	34.6	37.0
150	35.448127899789064	37.0	37.0	37.0	37.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	4927.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	49.0
13	257.0
14	464.0
15	626.0
16	850.0
17	964.0
18	1076.0
19	1176.0
20	1517.0
21	1962.0
22	2546.0
23	3231.0
24	4290.0
25	5457.0
26	7031.0
27	8884.0
28	11093.0
29	14564.0
30	19841.0
31	27769.0
32	39887.0
33	59861.0
34	102225.0
35	255322.0
36	1191017.0
37	233114.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	36.935340210608835	8.948043505174999	13.904253127579794	40.21236315663637
2	30.773422008176425	14.456211616618312	29.52645708981035	25.243909285394917
3	31.988954790125472	19.614570494412988	20.222818914395614	28.173655801065927
4	32.693690907550746	26.629050666316473	17.013913776588627	23.66334464954415
5	30.698626065311895	29.331508170377724	19.36525630891702	20.604609455393362
6	25.37731702047995	32.48126760273935	20.48722026712807	21.65419510965263
7	21.07481781368401	16.914819658228044	37.81931788962108	24.191044638466863
8	22.74869140126702	20.239660403403786	26.362042892666082	30.64960530266311
9	25.395612090384663	19.88894641950445	27.46912017755741	27.246321312553473
10-14	26.53106928919393	24.15216886800633	23.680406681860763	25.636355160938972
15-19	26.86547309296452	24.10632593393826	23.7551608387262	25.273040134371023
20-24	26.32860646530486	23.76813802568258	24.026587818143817	25.876667690868743
25-29	25.988352275005816	24.164918872963803	23.93491189515644	25.91181695687394
30-34	25.647152600747475	24.083209882113486	24.428321840463425	25.841315676675613
35-39	25.687755902234944	24.454120358160342	24.40973639374774	25.448387345856975
40-44	26.154507826473854	24.14170370501875	24.01049099850944	25.693297469997955
45-49	25.62661043801105	24.068610892091485	24.60525049819928	25.699528171698187
50-54	26.67199835231642	23.283105883543218	24.535018194176537	25.50987756996382
55-59	25.78671193470443	23.566687898613736	24.885318359688466	25.761281806993363
60-64	27.376289489030547	23.77452794144262	23.65992680327374	25.189255766253094
65-69	26.380323980164285	23.935979492294905	24.403631230337417	25.280065297203397
70-74	26.5613369888761	24.160187599928364	23.943242860232257	25.335232550963276
75-79	26.17565919862544	24.306952739045588	23.75415257110116	25.763235491227814
80-84	26.25893128435787	24.751920403654104	23.62675277126002	25.362395540728006
85-89	26.439661887803425	24.73802416072242	23.53530446387629	25.287009487597867
90-94	26.42081264351418	24.597112841922765	23.387367253638487	25.594707260924572
95-99	26.66566793570324	24.080970723754756	23.996000232087408	25.257361108454596
100-104	26.97870801996089	24.342687489386876	23.471069309859384	25.20753518079285
105-109	26.65796227857521	24.220112355057704	23.925342420416296	25.196582945950784
110-114	26.6256277719192	24.159332533076057	23.78666599355307	25.428373701451683
115-119	26.53635643542217	23.524928901474613	23.96880098756526	25.969913675537953
120-124	26.744788979220495	24.62949726145608	22.95686769581808	25.668846063505345
125-129	26.09448215797507	23.99439670414275	23.754565273794768	26.156555864087412
130-134	26.79383471963524	24.205878946040578	23.25811094908411	25.742175385240067
135-139	26.014088400677878	24.447292492574814	24.064940337150563	25.47367876959675
140-144	26.61427503715274	24.623156329929465	23.05745919905562	25.705109433862173
145-149	27.16010512812141	23.13319078023947	24.052709096693228	25.65399499494589
150	29.568287702368895	0.0	35.22222997236406	35.20948232526704
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	4953.0
1	2491.0
2	36.0
3	42.5
4	52.5
5	71.0
6	82.0
7	95.0
8	113.5
9	118.0
10	104.5
11	93.0
12	97.0
13	108.0
14	108.0
15	112.5
16	127.0
17	149.5
18	168.0
19	205.5
20	307.0
21	501.0
22	762.0
23	1092.0
24	1729.0
25	2412.0
26	2723.0
27	3395.0
28	4661.0
29	6137.0
30	7331.5
31	7893.5
32	8387.0
33	9116.0
34	9492.5
35	9734.5
36	9768.0
37	9550.5
38	10025.5
39	11313.5
40	12263.0
41	12359.0
42	13571.5
43	15746.0
44	17819.5
45	22168.5
46	31695.0
47	51234.5
48	75979.5
49	94799.5
50	111262.5
51	132509.5
52	155428.5
53	173827.5
54	182678.5
55	170713.0
56	153792.0
57	129593.0
58	91172.5
59	61836.5
60	42580.5
61	34017.5
62	29320.5
63	24290.5
64	17511.0
65	9163.5
66	4927.5
67	2737.5
68	1908.0
69	1485.5
70	1199.5
71	1066.5
72	980.0
73	892.0
74	900.5
75	965.5
76	954.0
77	981.0
78	950.5
79	781.5
80	667.0
81	615.5
82	584.0
83	509.5
84	429.0
85	400.5
86	406.0
87	398.5
88	408.5
89	418.5
90	431.0
91	484.0
92	502.0
93	530.5
94	630.0
95	722.0
96	777.0
97	875.5
98	1025.0
99	1224.0
100	2057.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.24634999999999999
2	0.24979999999999997
3	0.24634999999999999
4	0.24634999999999999
5	0.24634999999999999
6	0.24634999999999999
7	0.24634999999999999
8	0.24634999999999999
9	0.24634999999999999
10-14	0.24634999999999999
15-19	0.24634999999999999
20-24	0.24755
25-29	0.2552
30-34	0.26425
35-39	0.054482553054841565
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	1.2051016114290083E-4
105-109	1.12955970779194E-5
110-114	0.0
115-119	0.0
120-124	1.2662321464432932E-5
125-129	4.892875774512481E-4
130-134	8.303550113654842E-5
135-139	0.0
140-144	0.0
145-149	0.0
150	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
35-39	5815.0
40-44	456.0
45-49	710.0
50-54	1050.0
55-59	1782.0
60-64	2804.0
65-69	4567.0
70-74	7544.0
75-79	11396.0
80-84	17341.0
85-89	24505.0
90-94	33809.0
95-99	42540.0
100-104	52378.0
105-109	57396.0
110-114	62180.0
115-119	67564.0
120-124	67353.0
125-129	66556.0
130-134	67948.0
135-139	66161.0
140-144	65214.0
145-149	441405.0
150-151	831526.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	20.78186571890633
#Duplication Level	Percentage of total
1	16.94864789573159
2	2.701464565904089
3	1.6101413427540063
4	1.2527418195316735
5	1.037014824260964
6	0.9361193495099597
7	0.8467918411368627
8	0.768981021828418
9	0.7037301600202712
>10	14.141732696307699
>50	9.645778778109777
>100	31.452375778885706
>500	11.16441426051631
>1k	6.790065665502652
>5k	0.0
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN	4927	0.24634999999999999	No Hit
CGGGAACGTATTCACCGCAGCATTGCTGATCTGCGATTACTAGCGACTCC	4293	0.21465	No Hit
CCGGGAACGTATTCACCGCAGCATTGCTGATCTGCGATTACTAGCGACTC	3601	0.18005	No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG	3237	0.16185	No Hit
GGCCAATCAAACTCCGTGATAGCTGGTTCTCCCCGAAATGCATTTAGGTG	3230	0.16149999999999998	No Hit
CGGCGAATTAGTACCAGTCACCTCCACGCCTTACAACGCTTCCAGATCTA	2777	0.13885	No Hit
CGCGCCTCCACAACCTCTTACAGCTGCTTCACACTGGCCATGGGTAGATC	2600	0.13	No Hit
CGGCCTTCGGGTTGTAAACTCCTTTCGCCAGGGACGAAGCGTTTTGTGAC	2556	0.1278	No Hit
CGTCAAGTCATCATGCCCCTTATGTCCAGGGCTTCACACATGCTACAATG	2361	0.11804999999999999	No Hit
GGGTGATCTGCCCTGCACTCTGGGATAAGCCTGGGAAACTGGGTCTAATA	2195	0.10975	No Hit
CGGCGATCTGGGCTGTTTCCCTCTCGACTATGAAGCTTATCCCCCACAGT	2170	0.1085	No Hit
GTCAAGTCATCATGCCCCTTATGTCCAGGGCTTCACACATGCTACAATGG	2030	0.1015	No Hit
GCCGAATATGCACTCGCTAGAGGCTTTTCTCGACAGCACAAGCACACCAC	2012	0.1006	No Hit
CGCCGGATGACTAAGGGTTCCTGGGTCAAGTTCGTCTTCCCAGGGTGAGT	2011	0.10055000000000001	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	2.0E-4	0.0	0.0	0.0	0.0017	0.83445
2	2.0E-4	0.0	0.0	0.0	0.00275	0.8371
3	2.0E-4	0.0	0.0	0.0	0.0042	0.83735
4	2.0E-4	0.0	0.0	0.0	0.0061	0.83775
5	2.0E-4	0.0	0.0	0.0	0.0084	0.83835
6	2.0E-4	0.0	0.0	0.0	0.0115	0.83855
7	2.0E-4	0.0	0.0	0.0	0.01435	0.83885
8	2.0E-4	0.0	0.0	0.0	0.01855	0.8395
9	2.0E-4	0.0	0.0	0.0	0.02275	0.84125
10-11	2.0E-4	0.0	0.0	0.0	0.028675	0.8445750000000001
12-13	2.0E-4	0.0	0.0	0.0	0.034625	0.851525
14-15	2.0E-4	0.0	0.0	0.0	0.040125	0.8559749999999999
16-17	2.5E-4	0.0	0.0	0.0	0.046325000000000005	0.85705
18-19	2.5E-4	0.0	0.0	0.0	0.0531	0.8576250000000001
20-21	2.5E-4	0.0	0.0	0.0	0.06125	0.85805
22-23	2.7499999999999996E-4	0.0	0.0	0.0	0.06775	0.8584750000000001
24-25	3.5E-4	5.0E-5	0.0	0.0	0.08055	0.8591500000000001
26-27	3.75E-4	5.0E-5	0.0	0.0	0.08785000000000001	0.8598250000000001
28-29	4.0E-4	5.0E-5	0.0	0.0	0.092	0.8605499999999999
30-31	4.0E-4	5.0E-5	0.0	0.0	0.096625	0.861075
32-33	4.0E-4	5.0E-5	0.0	0.0	0.10155	0.86165
34-35	4.0E-4	5.0E-5	0.0	0.0	0.108975	0.8619749999999999
36-37	4.2500000000000003E-4	5.0E-5	0.0	0.0	0.11382500000000001	0.8623
38-39	4.75E-4	5.0E-5	0.0	0.0	0.1184	0.8626750000000001
40-41	5.0E-4	5.0E-5	0.0	0.0	0.122225	0.8632
42-43	5.250000000000001E-4	5.0E-5	0.0	0.0	0.12564999999999998	0.8634999999999999
44-45	5.5E-4	5.0E-5	0.0	0.0	0.13055	0.863925
46-47	5.75E-4	5.0E-5	0.0	0.0	0.135125	0.864325
48-49	6.0E-4	5.0E-5	0.0	0.0	0.138125	0.8647
50-51	6.0E-4	5.0E-5	0.0	0.0	0.14032499999999998	0.865
52-53	7.5E-4	5.0E-5	0.0	0.0	0.14277499999999999	0.8656
54-55	8.0E-4	5.0E-5	0.0	0.0	0.146125	0.86615
56-57	9.0E-4	5.0E-5	0.0	0.0	0.149425	0.8665499999999999
58-59	9.5E-4	5.0E-5	0.0	0.0	0.15225	0.8672249999999999
60-61	0.0010999999999999998	5.0E-5	0.0	0.0	0.1558	0.8679250000000001
62-63	0.00145	5.0E-5	0.0	0.0	0.160475	0.86825
64-65	0.0016749999999999998	5.0E-5	0.0	0.0	0.169025	0.8686
66-67	0.001775	5.0E-5	0.0	0.0	0.183175	0.868825
68-69	0.001925	5.0E-5	0.0	0.0	0.198875	0.869075
70-71	0.0021	5.0E-5	0.0	0.0	0.21760000000000002	0.8696
72-73	0.002125	5.0E-5	0.0	0.0	0.22397499999999998	0.8701
74-75	0.0023	5.0E-5	0.0	0.0	0.225925	0.8707
76-77	0.0026249999999999997	5.0E-5	0.0	0.0	0.228925	0.8711
78-79	0.002975	5.0E-5	0.0	0.0	0.23135	0.872025
80-81	0.003425	5.0E-5	0.0	0.0	0.23349999999999999	0.8728
82-83	0.0037	5.0E-5	0.0	0.0	0.2359	0.8738250000000001
84-85	0.00395	5.0E-5	0.0	0.0	0.23862499999999998	0.875675
86-87	0.004725	5.0E-5	0.0	0.0	0.241375	0.877525
88-89	0.00515	5.0E-5	0.0	0.0	0.242925	0.8796999999999999
90-91	0.00585	5.0E-5	0.0	0.0	0.245025	0.882425
92-93	0.0064	5.0E-5	0.0	0.0	0.2471	0.884875
94-95	0.00715	5.0E-5	0.0	0.0	0.2497	0.887425
96-97	0.007925	5.0E-5	0.0	0.0	0.25205	0.890675
98-99	0.008575	5.0E-5	0.0	5.0E-5	0.25434999999999997	0.8936999999999999
100-101	0.009399999999999999	5.0E-5	0.0	5.0E-5	0.25595	0.896075
102-103	0.010325	5.0E-5	0.0	5.0E-5	0.2577	0.898325
104-105	0.011575	5.0E-5	0.0	5.0E-5	0.259775	0.900875
106-107	0.01295	5.0E-5	0.0	5.0E-5	0.26145	0.903575
108-109	0.014624999999999999	5.0E-5	0.0	5.0E-5	0.263475	0.9064
110-111	0.015825	5.0E-5	0.0	5.0E-5	0.265225	0.9092
112-113	0.0174	5.0E-5	0.0	5.0E-5	0.267075	0.9118999999999999
114-115	0.018725	5.0E-5	0.0	5.0E-5	0.269175	0.9145
116-117	0.020275	5.0E-5	0.0	5.0E-5	0.27095	0.916975
118-119	0.021525000000000002	5.0E-5	0.0	5.0E-5	0.27312499999999995	0.918725
120-121	0.02265	5.0E-5	0.0	5.0E-5	0.27495	0.921225
122-123	0.025349999999999998	5.0E-5	2.5E-5	5.0E-5	0.27625	0.923875
124-125	0.02695	5.0E-5	5.0E-5	5.0E-5	0.27785	0.9257
126-127	0.028499999999999998	5.0E-5	5.0E-5	5.0E-5	0.27945	0.927875
128-129	0.030225000000000002	5.0E-5	5.0E-5	1.0E-4	0.281025	0.929925
130-131	0.031575000000000006	5.0E-5	5.0E-5	1.0E-4	0.28247500000000003	0.932375
132-133	0.035625	5.0E-5	5.0E-5	1.0E-4	0.284375	0.935175
134-135	0.0393	5.0E-5	5.0E-5	1.0E-4	0.2859	0.93805
136-137	0.040975	5.0E-5	5.0E-5	1.0E-4	0.28725	0.940275
138-139	0.04105	5.0E-5	5.0E-5	1.0E-4	0.28825	0.942975
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGCGAA	945	0.0	47.807163	1
CTGGCTC	800	0.0	40.920864	9
GATCCTG	955	0.0	34.27926	5
TCCGATC	720	0.0	33.646885	2
CCCCCCT	140	1.4675243E-6	32.73751	1
TGATCCT	1095	0.0	31.690315	4
GGCGAAT	1405	0.0	31.222965	2
GGTTCGC	820	0.0	31.13978	4
GTCCGAT	170	1.915505E-7	30.811779	1
TTTGATC	1455	0.0	30.600012	2
CGATCAA	855	0.0	28.333511	4
ATTAGTA	1970	0.0	27.25288	7
AATTAGT	1905	0.0	27.151691	6
GTTCGCG	1120	0.0	26.891527	1
CGGGTTC	320	5.456968E-12	26.598562	3
CGCGCCT	1340	0.0	26.38546	1
TCGCGCC	1140	0.0	25.844757	3
CCGCCAT	130	0.0011758801	25.182703	1
GCGAATT	1820	0.0	24.462584	3
GGCGATC	1905	0.0	24.059065	2
>>END_MODULE
