FastQCFastQC Report
Tue 21 Jul 2026
SRR12904818_R2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR12904818_R2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2000000
Total Bases273.6 Mbp
Sequences flagged as poor quality0
Sequence length35-150
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN49270.24634999999999999No Hit
CGGGAACGTATTCACCGCAGCATTGCTGATCTGCGATTACTAGCGACTCC42930.21465No Hit
CCGGGAACGTATTCACCGCAGCATTGCTGATCTGCGATTACTAGCGACTC36010.18005No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG32370.16185No Hit
GGCCAATCAAACTCCGTGATAGCTGGTTCTCCCCGAAATGCATTTAGGTG32300.16149999999999998No Hit
CGGCGAATTAGTACCAGTCACCTCCACGCCTTACAACGCTTCCAGATCTA27770.13885No Hit
CGCGCCTCCACAACCTCTTACAGCTGCTTCACACTGGCCATGGGTAGATC26000.13No Hit
CGGCCTTCGGGTTGTAAACTCCTTTCGCCAGGGACGAAGCGTTTTGTGAC25560.1278No Hit
CGTCAAGTCATCATGCCCCTTATGTCCAGGGCTTCACACATGCTACAATG23610.11804999999999999No Hit
GGGTGATCTGCCCTGCACTCTGGGATAAGCCTGGGAAACTGGGTCTAATA21950.10975No Hit
CGGCGATCTGGGCTGTTTCCCTCTCGACTATGAAGCTTATCCCCCACAGT21700.1085No Hit
GTCAAGTCATCATGCCCCTTATGTCCAGGGCTTCACACATGCTACAATGG20300.1015No Hit
GCCGAATATGCACTCGCTAGAGGCTTTTCTCGACAGCACAAGCACACCAC20120.1006No Hit
CGCCGGATGACTAAGGGTTCCTGGGTCAAGTTCGTCTTCCCAGGGTGAGT20110.10055000000000001No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGCGAA9450.047.8071631
CTGGCTC8000.040.9208649
GATCCTG9550.034.279265
TCCGATC7200.033.6468852
CCCCCCT1401.4675243E-632.737511
TGATCCT10950.031.6903154
GGCGAAT14050.031.2229652
GGTTCGC8200.031.139784
GTCCGAT1701.915505E-730.8117791
TTTGATC14550.030.6000122
CGATCAA8550.028.3335114
ATTAGTA19700.027.252887
AATTAGT19050.027.1516916
GTTCGCG11200.026.8915271
CGGGTTC3205.456968E-1226.5985623
CGCGCCT13400.026.385461
TCGCGCC11400.025.8447573
CCGCCAT1300.001175880125.1827031
GCGAATT18200.024.4625843
GGCGATC19050.024.0590652
Produced by FastQC (version 0.12.1)