##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	SRR12904821_R2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2000000
Total Bases	277.8 Mbp
Sequences flagged as poor quality	0
Sequence length	35-150
%GC	54
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	35.4761925	37.0	37.0	37.0	37.0	37.0
2	35.60569	37.0	37.0	37.0	37.0	37.0
3	35.8674325	37.0	37.0	37.0	37.0	37.0
4	35.8600005	37.0	37.0	37.0	37.0	37.0
5	35.8776625	37.0	37.0	37.0	37.0	37.0
6	35.8956115	37.0	37.0	37.0	37.0	37.0
7	35.8189605	37.0	37.0	37.0	37.0	37.0
8	35.8417285	37.0	37.0	37.0	37.0	37.0
9	35.9197855	37.0	37.0	37.0	37.0	37.0
10-14	35.894859100000005	37.0	37.0	37.0	37.0	37.0
15-19	35.898453700000005	37.0	37.0	37.0	37.0	37.0
20-24	35.8697255	37.0	37.0	37.0	37.0	37.0
25-29	35.7398051	37.0	37.0	37.0	37.0	37.0
30-34	35.72055300000001	37.0	37.0	37.0	37.0	37.0
35-39	35.780600430463	37.0	37.0	37.0	37.0	37.0
40-44	35.7943485489212	37.0	37.0	37.0	37.0	37.0
45-49	35.76553207146498	37.0	37.0	37.0	37.0	37.0
50-54	35.74836012551786	37.0	37.0	37.0	37.0	37.0
55-59	35.7560159988403	37.0	37.0	37.0	37.0	37.0
60-64	35.77548569839245	37.0	37.0	37.0	37.0	37.0
65-69	35.73476905360107	37.0	37.0	37.0	37.0	37.0
70-74	35.70183555919801	37.0	37.0	37.0	37.0	37.0
75-79	35.67980418790654	37.0	37.0	37.0	37.0	37.0
80-84	35.70685919098225	37.0	37.0	37.0	37.0	37.0
85-89	35.78909473491146	37.0	37.0	37.0	37.0	37.0
90-94	35.8021099325173	37.0	37.0	37.0	37.0	37.0
95-99	35.79866983834817	37.0	37.0	37.0	37.0	37.0
100-104	35.78504831005148	37.0	37.0	37.0	37.0	37.0
105-109	35.74897977901887	37.0	37.0	37.0	37.0	37.0
110-114	35.74960812142054	37.0	37.0	37.0	37.0	37.0
115-119	35.72161561652777	37.0	37.0	37.0	37.0	37.0
120-124	35.64605020530086	37.0	37.0	37.0	37.0	37.0
125-129	35.6587070021469	37.0	37.0	37.0	37.0	37.0
130-134	35.602759479250004	37.0	37.0	37.0	37.0	37.0
135-139	35.62246764703134	37.0	37.0	37.0	37.0	37.0
140-144	35.52324193083318	37.0	37.0	37.0	37.0	37.0
145-149	35.450540527577495	37.0	37.0	37.0	37.0	37.0
150	35.58869926876342	37.0	37.0	37.0	37.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	5713.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	41.0
13	219.0
14	449.0
15	771.0
16	1094.0
17	1322.0
18	1447.0
19	1733.0
20	2007.0
21	2531.0
22	3101.0
23	3856.0
24	4938.0
25	6216.0
26	7239.0
27	8017.0
28	9773.0
29	12716.0
30	17569.0
31	25091.0
32	36619.0
33	55294.0
34	93650.0
35	231630.0
36	1217390.0
37	249574.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.70461623627893	8.334507520732974	12.372893169338214	39.58798307364988
2	34.7711276434	12.516773542632379	25.45902842827142	27.253070385696205
3	33.995909314958176	17.069007620267293	18.497939363792675	30.437143700981856
4	34.68422549011251	22.6139968820937	16.857904604502764	25.84387302329103
5	32.92986415696436	26.503005836171024	18.68702949976608	21.880100507098525
6	27.246930857995867	31.160860999444917	18.949529330532666	22.642678812026553
7	23.813272613219663	17.27850605253908	35.096553304514345	23.811668029726913
8	24.55609448389324	20.03362605281988	25.447290184411774	29.962989278875106
9	26.398507336205874	18.865439126865894	26.87847837347383	27.857575163454406
10-14	27.871545068488135	22.886445130515316	22.65798252708863	26.584027273907918
15-19	27.97168110708238	23.34896632229965	22.588925265019526	26.09042730559844
20-24	27.741583507540856	22.570044462407026	22.817653987501185	26.87071804255093
25-29	27.17643508452541	23.003304954101424	23.023824894900493	26.796435066472675
30-34	27.398682100999338	22.931795677509538	22.81264594062393	26.85687628086719
35-39	27.099634160581715	23.41227819204623	23.067866683092056	26.420220964279995
40-44	27.388433481900393	22.953064066447112	22.779051873854357	26.879450577798142
45-49	27.06361089341555	22.88090559211804	23.17519854286652	26.880284971599888
50-54	27.855226786474308	22.116373886354296	23.604082956759026	26.42431637041237
55-59	26.827140390865868	22.156312589815883	23.949194349516	27.067352669802254
60-64	28.339599248291346	22.798805295752462	22.505147731910427	26.35644772404577
65-69	27.887463334210604	22.76652552799089	23.066237479393987	26.27977365840452
70-74	27.739999359879874	22.77424225275436	22.981362508810484	26.504395878555282
75-79	26.961397242851277	22.999561456661016	23.00019981688181	27.038841483605896
80-84	27.571100530626513	23.267469409336844	22.520097081920813	26.641332978115834
85-89	27.842833866194955	23.30854740142744	22.404597932305368	26.44402080007223
90-94	27.783477195817184	23.188955758526745	22.536373545343388	26.491193500312683
95-99	27.843075362891355	22.764112511797364	22.664595608874567	26.728216516436714
100-104	28.709471191639192	22.615591724947805	22.419722469553566	26.25521461385944
105-109	27.989750319958784	22.869760826133803	22.98773531654252	26.15275353736489
110-114	28.2651257443202	22.63338911809041	22.846263026770686	26.2552221108187
115-119	27.910013254597715	22.502076584822408	22.575891224371247	27.012018936208626
120-124	28.327313843190577	23.140131142362282	21.891851392630198	26.64070362181694
125-129	27.707001811200723	22.734109557429726	22.342327230962116	27.21656140040744
130-134	28.411399487029087	22.76928068822836	22.057475139470736	26.761844685271814
135-139	27.296398163346602	23.435528153455028	22.501996199774208	26.766077483424162
140-144	27.83084023593871	23.996503331566867	21.578682618410262	26.59397381408416
145-149	28.579742279786657	22.085124650941275	22.46978574253377	26.86534732673829
150	31.29420719275831	0.0	32.861065050153584	35.8447277570881
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	5729.0
1	2879.0
2	28.5
3	34.0
4	50.5
5	59.0
6	65.0
7	83.0
8	99.5
9	106.5
10	108.5
11	111.0
12	128.0
13	130.0
14	113.5
15	115.5
16	119.0
17	124.0
18	122.5
19	128.0
20	138.0
21	156.0
22	171.0
23	172.5
24	194.5
25	220.0
26	250.0
27	279.5
28	379.0
29	630.0
30	771.5
31	715.0
32	739.5
33	873.0
34	1003.0
35	1137.5
36	1317.5
37	1599.5
38	1941.5
39	2536.0
40	3524.5
41	4369.5
42	5387.0
43	7149.5
44	9236.5
45	11904.0
46	16406.5
47	29995.5
48	52789.5
49	76698.5
50	92164.0
51	117913.0
52	152463.0
53	170198.5
54	187513.0
55	195262.0
56	185972.5
57	166228.0
58	130743.5
59	92058.5
60	60756.0
61	46403.5
62	41787.0
63	37129.5
64	28648.0
65	16423.0
66	9112.5
67	5684.5
68	4244.0
69	3485.0
70	2959.0
71	2487.0
72	2198.0
73	1839.5
74	1586.0
75	1512.5
76	1365.0
77	1216.5
78	1140.5
79	977.0
80	811.5
81	799.0
82	814.5
83	715.5
84	612.0
85	575.0
86	577.5
87	617.5
88	642.0
89	682.5
90	694.0
91	713.5
92	751.5
93	798.0
94	894.5
95	1015.5
96	1104.5
97	1166.0
98	1236.5
99	1266.5
100	2050.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.28565
2	0.2894
3	0.28565
4	0.28565
5	0.28565
6	0.28565
7	0.28565
8	0.28565
9	0.28565
10-14	0.28565
15-19	0.28565
20-24	0.28654999999999997
25-29	0.29211
30-34	0.30192
35-39	0.0620181835790367
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	2.467942235129441E-4
105-109	2.199784905031986E-5
110-114	0.0
115-119	0.0
120-124	2.4188415176247076E-5
125-129	7.15636365827786E-4
130-134	7.831212314737989E-5
135-139	0.0
140-144	0.0
145-149	1.510014263594734E-5
150	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
35-39	6548.0
40-44	376.0
45-49	705.0
50-54	1063.0
55-59	1687.0
60-64	2549.0
65-69	3902.0
70-74	6137.0
75-79	8549.0
80-84	12507.0
85-89	17995.0
90-94	25873.0
95-99	32407.0
100-104	41996.0
105-109	49052.0
110-114	54308.0
115-119	57879.0
120-124	58876.0
125-129	61141.0
130-134	60974.0
135-139	60715.0
140-144	57876.0
145-149	457210.0
150-151	919675.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	18.983705471640157
#Duplication Level	Percentage of total
1	15.713731399961217
2	2.2113355709778615
3	1.3550438629247992
4	1.030234450573183
5	0.8886928997525814
6	0.786128094688162
7	0.6973672034551457
8	0.6609856136880075
9	0.5797068606266835
>10	12.921935172027307
>50	8.806864141409717
>100	32.12908581301207
>500	12.651155629009109
>1k	8.982545472689456
>5k	0.5851878152047091
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGGCCTTCGGGTTGTAAACTCCTTTCGCCAGGGACGAAGCGTTTTGTGAC	5992	0.2996	No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN	5713	0.28565	No Hit
GGCCAATCAAACTCCGTGATAGCTGGTTCTCCCCGAAATGCATTTAGGTG	3867	0.19335000000000002	No Hit
CGGCGAGCGAACGCGAATGTTGGCTAAACTGCATGTGTGTGATACCTGGT	3405	0.17025	No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG	3198	0.1599	No Hit
CGCGAATGTTGGCTAAACTGCATGTGTGTGATACCTGGTAGGGGTTGCAT	2890	0.14450000000000002	No Hit
CCGGGAACGTATTCACCGCAGCATTGCTGATCTGCGATTACTAGCGACTC	2713	0.13565	No Hit
GTGGAAGGCTGCGTTATGCCTCGGGGAGTTGTCAATCGAGCGTTGATCCG	2707	0.13535	No Hit
CCCATACTGACTATAAATTCACCAGCAGGTGACTGCGAATAACCACAGCC	2691	0.13455	No Hit
GTGGGTTCGCGCCTCCACAACCTCTTACAGCTGCTTCACACTGGCCATGG	2561	0.12805	No Hit
GTTTGATCCTGGCTCAGGACGAACGCTGGCGGCGTGCTTAACACATGCAA	2401	0.12004999999999999	No Hit
CGGGAACGTATTCACCGCAGCATTGCTGATCTGCGATTACTAGCGACTCC	2290	0.11449999999999999	No Hit
GCCTGATGCAGCGACGCCGCGTGGGGGATGACGGCCTTCGGGTTGTAAAC	2200	0.11	No Hit
CGCGCCTCCACAACCTCTTACAGCTGCTTCACACTGGCCATGGGTAGATC	2193	0.10965	No Hit
CCCTACAGTACTCAAGTCAGCCCGTATCGCCTGCACGCCCACAGTTAAGC	2154	0.1077	No Hit
CGGCGATCTGGGCTGTTTCCCTCTCGACTATGAAGCTTATCCCCCACAGT	2142	0.10709999999999999	No Hit
GTCCGGAATTACTGGGCGTAAAGAGCTCGTAGGTGGTTTGTCACGTCGTC	2141	0.10705	No Hit
GCCGTATCTCAGTCCCAATGTGGCCGACCAACCTCTCAGTCCGGCTACCC	2103	0.10515	No Hit
GGGTGAAAGGCCAATCAAACTCCGTGATAGCTGGTTCTCCCCGAAATGCA	2100	0.105	No Hit
CTGCGTTATGCCTCGGGGAGTTGTCAATCGAGCGTTGATCCGAGGATGTC	2033	0.10165	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	5.0E-5	0.0	0.0	0.0	7.5E-4	1.13995
2	5.0E-5	0.0	0.0	0.0	0.00155	1.1424
3	5.0E-5	0.0	0.0	0.0	0.00235	1.1425
4	5.0E-5	0.0	0.0	0.0	0.0036	1.14285
5	5.0E-5	0.0	0.0	0.0	0.00525	1.14345
6	5.0E-5	0.0	0.0	0.0	0.0079	1.1438
7	5.0E-5	0.0	0.0	0.0	0.011	1.14435
8	5.0E-5	5.0E-5	0.0	0.0	0.01465	1.1451
9	5.0E-5	5.0E-5	0.0	0.0	0.01805	1.1474
10-11	5.0E-5	5.0E-5	0.0	0.0	0.0241	1.1518
12-13	5.0E-5	5.0E-5	0.0	0.0	0.0292	1.1625
14-15	7.500000000000001E-5	5.0E-5	0.0	0.0	0.034475000000000006	1.1691500000000001
16-17	1.0E-4	5.0E-5	0.0	0.0	0.0413	1.17095
18-19	1.0E-4	5.0E-5	0.0	0.0	0.049350000000000005	1.1718000000000002
20-21	1.0E-4	5.0E-5	0.0	0.0	0.057499999999999996	1.173125
22-23	1.0E-4	5.0E-5	0.0	0.0	0.064975	1.17445
24-25	1.0E-4	5.0E-5	0.0	0.0	0.0858	1.17535
26-27	1.25E-4	5.0E-5	0.0	0.0	0.094525	1.17595
28-29	1.75E-4	5.0E-5	0.0	0.0	0.0997	1.1769
30-31	2.0E-4	5.0E-5	0.0	0.0	0.1045	1.177575
32-33	2.0E-4	5.0E-5	0.0	0.0	0.11052500000000001	1.178
34-35	2.0E-4	5.0E-5	0.0	0.0	0.120175	1.1784249999999998
36-37	2.0E-4	5.0E-5	0.0	0.0	0.12755	1.1792500000000001
38-39	2.5E-4	5.0E-5	0.0	0.0	0.132775	1.179675
40-41	3.0E-4	5.0E-5	0.0	0.0	0.13672499999999999	1.180475
42-43	3.75E-4	1.0E-4	0.0	0.0	0.14075	1.1810999999999998
44-45	4.75E-4	1.0E-4	0.0	0.0	0.1469	1.18205
46-47	5.5E-4	1.0E-4	0.0	0.0	0.1513	1.182775
48-49	5.75E-4	1.0E-4	0.0	0.0	0.153975	1.18335
50-51	6.249999999999999E-4	1.0E-4	0.0	0.0	0.155725	1.1839
52-53	6.75E-4	1.0E-4	0.0	0.0	0.15785	1.1843499999999998
54-55	8.5E-4	1.0E-4	0.0	0.0	0.1609	1.1848
56-57	9.0E-4	1.0E-4	0.0	0.0	0.16375	1.185625
58-59	0.001	1.0E-4	0.0	0.0	0.167075	1.186525
60-61	0.0010999999999999998	1.0E-4	0.0	0.0	0.17065000000000002	1.1872
62-63	0.0012000000000000001	1.0E-4	0.0	0.0	0.175725	1.1878250000000001
64-65	0.00125	1.0E-4	0.0	0.0	0.187975	1.188475
66-67	0.00135	1.0E-4	0.0	0.0	0.21155	1.1889750000000001
68-69	0.0015249999999999999	1.0E-4	0.0	0.0	0.23572500000000002	1.189775
70-71	0.001725	1.0E-4	0.0	0.0	0.26335	1.190275
72-73	0.002	1.0E-4	0.0	0.0	0.27155	1.1907
74-75	0.002075	1.0E-4	0.0	0.0	0.274025	1.1911999999999998
76-77	0.002225	1.0E-4	0.0	0.0	0.276	1.1923
78-79	0.00245	1.0E-4	0.0	0.0	0.278025	1.19385
80-81	0.0026	1.0E-4	0.0	0.0	0.279925	1.1953
82-83	0.0027	1.0E-4	0.0	0.0	0.28205	1.197625
84-85	0.002875	1.0E-4	0.0	0.0	0.283975	1.2006000000000001
86-87	0.003225	1.0E-4	0.0	0.0	0.285675	1.203875
88-89	0.003775	1.0E-4	0.0	0.0	0.28705	1.20795
90-91	0.00435	1.0E-4	0.0	0.0	0.288375	1.2123
92-93	0.00485	1.0E-4	0.0	0.0	0.289975	1.2171
94-95	0.0056	1.0E-4	0.0	0.0	0.292275	1.222275
96-97	0.00625	1.0E-4	0.0	2.5E-5	0.2943	1.227225
98-99	0.0065249999999999996	1.0E-4	0.0	1.0E-4	0.296025	1.2314
100-101	0.007175	1.0E-4	0.0	1.25E-4	0.29775	1.2352750000000001
102-103	0.008074999999999999	1.5E-4	0.0	1.5E-4	0.299475	1.2386249999999999
104-105	0.00935	1.5E-4	0.0	1.5E-4	0.301025	1.2419250000000002
106-107	0.0102	1.5E-4	0.0	1.5E-4	0.302275	1.245775
108-109	0.011	1.5E-4	2.5E-5	2.0E-4	0.3042	1.2496749999999999
110-111	0.01195	1.5E-4	5.0E-5	2.0E-4	0.30562500000000004	1.2530999999999999
112-113	0.013575	1.5E-4	5.0E-5	2.0E-4	0.307125	1.25635
114-115	0.014475	1.5E-4	5.0E-5	2.0E-4	0.308125	1.2594249999999998
116-117	0.015825	1.5E-4	5.0E-5	2.0E-4	0.3095	1.26295
118-119	0.0169	1.5E-4	5.0E-5	2.0E-4	0.311	1.26675
120-121	0.01745	1.5E-4	5.0E-5	2.0E-4	0.31265	1.2701500000000001
122-123	0.01865	1.5E-4	5.0E-5	2.0E-4	0.31384999999999996	1.2734
124-125	0.020200000000000003	1.5E-4	5.0E-5	2.0E-4	0.31442499999999995	1.276125
126-127	0.021475	2.0E-4	5.0E-5	2.0E-4	0.316275	1.278625
128-129	0.0226	2.0E-4	5.0E-5	2.0E-4	0.31779999999999997	1.2818999999999998
130-131	0.023575	2.0E-4	5.0E-5	2.0E-4	0.31885	1.2860999999999998
132-133	0.02665	2.0E-4	5.0E-5	2.0E-4	0.32095	1.289675
134-135	0.029324999999999997	2.0E-4	5.0E-5	2.0E-4	0.32284999999999997	1.292875
136-137	0.030350000000000002	2.0E-4	5.0E-5	2.5E-4	0.324575	1.29605
138-139	0.0304	2.0E-4	5.0E-5	2.5E-4	0.3255	1.3000500000000001
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGGAAT	90	2.3799676E-6	44.389194	1
CGCAATA	415	0.0	41.715145	1
TTTGATC	860	0.0	40.259968	2
GCAATAG	425	0.0	39.166935	2
GGTTCGC	880	0.0	37.829803	4
GATCCTG	960	0.0	36.757957	5
GGCGTCT	300	0.0	33.290226	3
ATAGTAA	520	0.0	33.290226	5
CTGGCTC	1070	0.0	32.356853	9
TGATCCT	1120	0.0	31.50682	4
GGGTTCG	1155	0.0	31.128525	3
CGCGTTA	150	2.2056483E-6	31.072437	1
AGTACCA	775	0.0	30.068592	8
AATAGTA	555	0.0	29.991196	4
GATCCTA	440	0.0	28.750648	4
TGGATGC	1000	0.0	27.963789	3
GTCGGAT	120	6.68083E-4	27.743246	1
ATTAGTA	820	0.0	27.606527	5
CCTAAGA	470	0.0	26.915503	7
GAATTAG	775	0.0	26.632181	3
>>END_MODULE
