FastQCFastQC Report
Tue 21 Jul 2026
SRR12904817_R2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR12904817_R2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2000000
Total Bases266.4 Mbp
Sequences flagged as poor quality0
Sequence length35-150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN90960.4548No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG59660.2983No Hit
CGGGAACGTATTCACCGCGGCGTGCTGATCCGCGATTACTAGCGATTCCG27350.13675No Hit
GCGAAATTCCTTGTCGGGTAAGTTCCGACCTGCACGAATGGCATAATGAT20230.10115No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGAGCT4400.039.2167973
CCTCGAG4650.038.4846841
TTTACTC7100.036.0067982
TGAACGC5500.033.69748
CGAGCTA5400.033.1379284
CGGAATT5400.027.221811
CTCTTCG9150.026.5413866
TTGATCA7100.026.1036173
GATTGAA8950.024.9922925
TACGAAG4350.024.9758769
TTTGATC9550.024.7617442
CAGATTG8350.024.4920183
GCGAAAT17150.024.2232321
TGGTTCG8050.023.8169673
ACGCTCA10750.023.780043
GCCGTAT1350.001686400323.6711391
AACGTAT32000.023.5663915
CGAAATT16550.023.5567152
CGCTCAT10600.023.5136364
CGTATTC32500.023.4004737
Produced by FastQC (version 0.12.1)