FastQCFastQC Report
Tue 21 Jul 2026
SRR12904821_R1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR12904821_R1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2000000
Total Bases275 Mbp
Sequences flagged as poor quality0
Sequence length35-150
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN297451.48725No Hit
CGGCCTTCGGGTTGTAAACTCCTTTCGCCAGGGACGAAGCGTTTTGTGAC60700.3035No Hit
GGCCAATCAAACTCCGTGATAGCTGGTTCTCCCCGAAATGCATTTAGGTG40030.20015No Hit
CGGCGAGCGAACGCGAATGTTGGCTAAACTGCATGTGTGTGATACCTGGT35620.1781No Hit
CGCGAATGTTGGCTAAACTGCATGTGTGTGATACCTGGTAGGGGTTGCAT28960.1448No Hit
GTGGAAGGCTGCGTTATGCCTCGGGGAGTTGTCAATCGAGCGTTGATCCG28180.1409No Hit
GTTTGATCCTGGCTCAGGACGAACGCTGGCGGCGTGCTTAACACATGCAA26270.13135No Hit
CCCATACTGACTATAAATTCACCAGCAGGTGACTGCGAATAACCACAGCC26090.13045No Hit
GTGGGTTCGCGCCTCCACAACCTCTTACAGCTGCTTCACACTGGCCATGG24340.1217No Hit
CGGCGATCTGGGCTGTTTCCCTCTCGACTATGAAGCTTATCCCCCACAGT22980.1149No Hit
GGGTGAAAGGCCAATCAAACTCCGTGATAGCTGGTTCTCCCCGAAATGCA22520.1126No Hit
CGCGCCTCCACAACCTCTTACAGCTGCTTCACACTGGCCATGGGTAGATC22140.11069999999999999No Hit
CGGTGTTCCTCCTGATATCTGCGCATTTCACCGCTACACCAGGAATTCCA22030.11015No Hit
GCCTGATGCAGCGACGCCGCGTGGGGGATGACGGCCTTCGGGTTGTAAAC21990.10995No Hit
CCGGGAACGTATTCACCGCAGCATTGCTGATCTGCGATTACTAGCGACTC21640.1082No Hit
GTCCGGAATTACTGGGCGTAAAGAGCTCGTAGGTGGTTTGTCACGTCGTC21540.1077No Hit
GCCGTATCTCAGTCCCAATGTGGCCGACCAACCTCTCAGTCCGGCTACCC21120.10560000000000001No Hit
CCCTACAGTACTCAAGTCAGCCCGTATCGCCTGCACGCCCACAGTTAAGC21070.10535No Hit
CGGAATTACTGGGCGTAAAGAGCTCGTAGGTGGTTTGTCACGTCGTCTGT20430.10215No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTGATC9450.042.946712
GATCCTG10500.038.0184025
GGCGTCT3350.037.734683
ACCGATT901.2413641E-436.962336
GGTTCGC10050.036.4106564
CTGGCTC10750.034.039739
TGATCCT11550.033.9861454
GTTATAT800.003104344533.26611
GCCCACT3000.033.2660981
CCGCCAT1807.410563E-933.2660981
GGGTTCG11850.032.564283
GCGTCTT3700.032.3670164
CGCGGTT1651.2809323E-732.2580381
CCCGCTA1651.2809323E-732.2580382
GTTTCGA3100.032.1929975
TGCAAAG5100.030.0047172
GCAAAGG5250.029.147443
GATCCTA3450.028.9270424
CTGCAAA5200.028.1482371
CGCAATA4550.027.7826771
Produced by FastQC (version 0.12.1)