FastQCFastQC Report
Tue 21 Jul 2026
SRR12904821_R2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR12904821_R2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2000000
Total Bases277.8 Mbp
Sequences flagged as poor quality0
Sequence length35-150
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGCCTTCGGGTTGTAAACTCCTTTCGCCAGGGACGAAGCGTTTTGTGAC59920.2996No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN57130.28565No Hit
GGCCAATCAAACTCCGTGATAGCTGGTTCTCCCCGAAATGCATTTAGGTG38670.19335000000000002No Hit
CGGCGAGCGAACGCGAATGTTGGCTAAACTGCATGTGTGTGATACCTGGT34050.17025No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG31980.1599No Hit
CGCGAATGTTGGCTAAACTGCATGTGTGTGATACCTGGTAGGGGTTGCAT28900.14450000000000002No Hit
CCGGGAACGTATTCACCGCAGCATTGCTGATCTGCGATTACTAGCGACTC27130.13565No Hit
GTGGAAGGCTGCGTTATGCCTCGGGGAGTTGTCAATCGAGCGTTGATCCG27070.13535No Hit
CCCATACTGACTATAAATTCACCAGCAGGTGACTGCGAATAACCACAGCC26910.13455No Hit
GTGGGTTCGCGCCTCCACAACCTCTTACAGCTGCTTCACACTGGCCATGG25610.12805No Hit
GTTTGATCCTGGCTCAGGACGAACGCTGGCGGCGTGCTTAACACATGCAA24010.12004999999999999No Hit
CGGGAACGTATTCACCGCAGCATTGCTGATCTGCGATTACTAGCGACTCC22900.11449999999999999No Hit
GCCTGATGCAGCGACGCCGCGTGGGGGATGACGGCCTTCGGGTTGTAAAC22000.11No Hit
CGCGCCTCCACAACCTCTTACAGCTGCTTCACACTGGCCATGGGTAGATC21930.10965No Hit
CCCTACAGTACTCAAGTCAGCCCGTATCGCCTGCACGCCCACAGTTAAGC21540.1077No Hit
CGGCGATCTGGGCTGTTTCCCTCTCGACTATGAAGCTTATCCCCCACAGT21420.10709999999999999No Hit
GTCCGGAATTACTGGGCGTAAAGAGCTCGTAGGTGGTTTGTCACGTCGTC21410.10705No Hit
GCCGTATCTCAGTCCCAATGTGGCCGACCAACCTCTCAGTCCGGCTACCC21030.10515No Hit
GGGTGAAAGGCCAATCAAACTCCGTGATAGCTGGTTCTCCCCGAAATGCA21000.105No Hit
CTGCGTTATGCCTCGGGGAGTTGTCAATCGAGCGTTGATCCGAGGATGTC20330.10165No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGAAT902.3799676E-644.3891941
CGCAATA4150.041.7151451
TTTGATC8600.040.2599682
GCAATAG4250.039.1669352
GGTTCGC8800.037.8298034
GATCCTG9600.036.7579575
GGCGTCT3000.033.2902263
ATAGTAA5200.033.2902265
CTGGCTC10700.032.3568539
TGATCCT11200.031.506824
GGGTTCG11550.031.1285253
CGCGTTA1502.2056483E-631.0724371
AGTACCA7750.030.0685928
AATAGTA5550.029.9911964
GATCCTA4400.028.7506484
TGGATGC10000.027.9637893
GTCGGAT1206.68083E-427.7432461
ATTAGTA8200.027.6065275
CCTAAGA4700.026.9155037
GAATTAG7750.026.6321813
Produced by FastQC (version 0.12.1)