Workshop Overview

Description

In this 2-day workshop, you will learn about various ways to study the microbiome including important concepts and best practices in microbial community analysis. You will also gain hands-on experience setting up and running a shotgun metagenomics pipeline using Nephele, NIAID’s free application for microbiome analysis, and will explore key outputs for downstream analysis.

Day 1: The Basics of Microbiome Analysis (8/12/2025)

• Learn about various omics approaches to studying the microbiome and key concepts.
• Understand best practices, available resources, and bioinformatic considerations for microbial community analysis.

Day 2: Shotgun Metagenomics with Nephele (8/14/2025)

• Gain hands-on experience running a shotgun metagenomics pipeline using Nephele.

Prerequisites

This workshop is intended for a beginner audience. No prior knowledge is required. Attendees will need to register for a Nephele account at https://nephele.niaid.nih.gov. See How to register for Nephele for further instructions.

Questions

If your question(s) remain unanswered following the workshop, please email us at ncibtep@nih.gov and we will submit your question to the appropriate speaker.

Recordings

All events will be recorded and made available on the BTEP Video Archive 24 to 48 hours following the event.