##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	S9_Air_HiSeq_cami_sub25k.R1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	25000
Total Bases	3.7 Mbp
Sequences flagged as poor quality	0
Sequence length	150
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.44752	35.0	32.0	35.0	32.0	35.0
2	33.62768	35.0	32.0	35.0	32.0	35.0
3	33.62528	35.0	32.0	35.0	32.0	35.0
4	37.0674	38.0	36.0	38.0	36.0	38.0
5	36.97988	38.0	36.0	38.0	36.0	38.0
6	36.95684	38.0	36.0	38.0	36.0	38.0
7	36.95128	38.0	36.0	38.0	36.0	38.0
8	36.94556	38.0	36.0	38.0	36.0	38.0
9	38.6286	40.0	38.0	40.0	36.0	40.0
10-14	38.862199999999994	40.2	38.2	40.4	36.0	40.4
15-19	39.916943999999994	41.0	39.0	42.0	36.0	42.0
20-24	39.787864	41.0	39.0	42.0	35.2	42.0
25-29	39.481975999999996	41.0	39.0	42.0	34.8	42.0
30-34	39.149719999999995	41.0	38.8	42.0	34.0	42.0
35-39	38.768392	41.0	37.8	42.0	33.6	42.0
40-44	38.042488	40.2	36.2	42.0	32.2	42.0
45-49	37.300984	39.4	35.8	41.4	31.0	42.0
50-54	36.613992	38.2	35.0	41.0	30.0	42.0
55-59	36.321912	37.0	35.0	40.8	30.6	42.0
60-64	35.726312	36.0	35.0	39.8	30.0	42.0
65-69	34.980608000000004	36.0	34.0	38.2	29.4	41.0
70-74	34.302688	36.0	34.0	36.8	28.2	39.8
75-79	33.67904	35.8	33.8	36.0	27.8	38.0
80-84	33.554864	36.0	34.0	36.0	28.0	37.0
85-89	33.174023999999996	36.0	34.0	36.0	27.4	36.4
90-94	32.864703999999996	36.0	33.8	36.0	26.2	36.0
95-99	32.561496	36.0	33.0	36.0	25.6	36.0
100-104	32.235256	35.8	32.8	36.0	24.8	36.0
105-109	31.906615999999996	35.4	32.2	36.0	22.8	36.0
110-114	31.472056	35.0	32.0	36.0	21.0	36.0
115-119	31.160584	35.0	31.2	36.0	19.6	36.0
120-124	30.867967999999998	35.0	31.0	36.0	17.6	36.0
125-129	30.307152000000002	35.0	30.4	36.0	11.8	36.0
130-134	29.706336	34.8	29.6	36.0	5.4	36.0
135-139	29.113896	34.0	28.0	36.0	3.0	36.0
140-144	28.349519999999995	34.0	27.2	36.0	3.0	36.0
145-149	27.191608000000002	34.0	24.2	36.0	3.6	36.0
150	24.65	31.0	16.0	35.0	3.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
31	18.0
32	654.0
33	7607.0
34	14168.0
35	2544.0
36	9.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	23.472	26.279999999999998	26.548	23.7
2	23.928	26.268	26.36	23.444000000000003
3	23.424	27.012000000000004	26.072	23.491999999999997
4	23.62	26.104	26.535999999999998	23.74
5	23.544	26.804	26.388	23.264000000000003
6	23.396	26.26	26.604	23.74
7	23.536	26.516000000000002	26.619999999999997	23.328
8	23.548	26.372	26.504	23.576
9	23.528	26.084000000000003	26.704	23.684
10-14	23.5992	26.494400000000002	26.296000000000003	23.610400000000002
15-19	23.640800000000002	26.3672	26.2704	23.721600000000002
20-24	23.505599999999998	26.4104	26.4712	23.6128
25-29	23.5808	26.4264	26.462400000000002	23.5304
30-34	23.6048	26.3552	26.482400000000002	23.5576
35-39	23.825599999999998	26.275199999999998	26.402399999999997	23.4968
40-44	23.3368	26.4232	26.5992	23.640800000000002
45-49	23.6064	26.4144	26.4936	23.4856
50-54	23.5952	26.38	26.4712	23.5536
55-59	23.528188225505804	26.407411259290075	26.339410715285723	23.7249897999184
60-64	23.6712	26.3312	26.419999999999998	23.5776
65-69	23.56	26.4064	26.476	23.5576
70-74	23.828990631925056	26.414611316890536	26.26021008168065	23.496187969503758
75-79	23.6728	26.3248	26.573600000000003	23.4288
80-84	23.448	26.6488	26.484	23.4192
85-89	23.6088	26.4424	26.378400000000003	23.5704
90-94	23.6696	26.4832	26.507199999999997	23.34
95-99	23.576800000000002	26.232	26.595999999999997	23.5952
100-104	23.7632	26.444000000000003	26.2488	23.544
105-109	23.500799999999998	26.173600000000004	26.499200000000002	23.8264
110-114	23.584	26.288800000000002	26.6256	23.5016
115-119	23.6424	26.422400000000003	26.2872	23.648
120-124	23.7104	26.323999999999998	26.1792	23.7864
125-129	23.5792	26.179999999999996	26.5856	23.6552
130-134	23.500988007904063	26.53941231529852	26.227409819278552	23.73218985751886
135-139	23.8064	26.190400000000004	26.2608	23.7424
140-144	23.863581817309075	26.308420934734954	26.314021024336387	23.51397622361958
145-149	23.832	26.029600000000002	26.4832	23.6552
150	23.488	26.423999999999996	26.156000000000002	23.932000000000002
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.5
13	1.5
14	1.5
15	3.0
16	8.5
17	11.5
18	20.0
19	29.5
20	43.0
21	68.0
22	84.5
23	112.0
24	150.0
25	188.0
26	240.0
27	297.0
28	344.5
29	385.5
30	446.0
31	494.0
32	532.5
33	578.0
34	619.5
35	633.0
36	608.0
37	568.5
38	541.0
39	516.0
40	449.5
41	425.0
42	407.0
43	389.0
44	408.5
45	408.5
46	436.5
47	492.5
48	563.5
49	659.0
50	704.5
51	769.0
52	866.5
53	929.0
54	983.5
55	1001.0
56	985.5
57	956.0
58	862.0
59	758.5
60	702.5
61	606.5
62	502.0
63	404.0
64	315.0
65	269.0
66	233.5
67	179.5
68	149.0
69	134.5
70	104.0
71	91.5
72	76.0
73	55.0
74	41.0
75	38.5
76	40.5
77	28.5
78	14.5
79	9.0
80	8.0
81	6.0
82	4.5
83	3.0
84	1.5
85	1.0
86	0.5
87	0.5
88	0.5
89	0.5
90	0.5
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	7.999999999999999E-4
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
150	25000.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	99.968
#Duplication Level	Percentage of total
1	99.936
2	0.064
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
>10	0.0
>50	0.0
>100	0.0
>500	0.0
>1k	0.0
>5k	0.0
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	0.0	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	0.0	0.0	0.0
16-17	0.0	0.0	0.0	0.0	0.0	0.0
18-19	0.0	0.0	0.0	0.0	0.0	0.0
20-21	0.0	0.0	0.0	0.0	0.0	0.0
22-23	0.0	0.0	0.0	0.0	0.0	0.0
24-25	0.0	0.0	0.0	0.0	0.0	0.0
26-27	0.0	0.0	0.0	0.0	0.0	0.0
28-29	0.0	0.0	0.0	0.0	0.0	0.0
30-31	0.0	0.0	0.0	0.0	0.0	0.0
32-33	0.0	0.0	0.0	0.0	0.0	0.0
34-35	0.0	0.0	0.0	0.0	0.0	0.0
36-37	0.0	0.0	0.0	0.0	0.0	0.0
38-39	0.0	0.0	0.0	0.0	0.0	0.0
40-41	0.0	0.0	0.0	0.0	0.0	0.0
42-43	0.0	0.0	0.0	0.0	0.0	0.0
44-45	0.0	0.0	0.0	0.0	0.0	0.0
46-47	0.0	0.0	0.0	0.0	0.0	0.0
48-49	0.0	0.0	0.0	0.0	0.0	0.0
50-51	0.0	0.0	0.0	0.0	0.0	0.0
52-53	0.0	0.0	0.0	0.0	0.0	0.0
54-55	0.0	0.0	0.0	0.0	0.0	0.0
56-57	0.0	0.0	0.0	0.0	0.0	0.0
58-59	0.0	0.0	0.0	0.0	0.0	0.0
60-61	0.0	0.0	0.0	0.0	0.0	0.0
62-63	0.0	0.0	0.0	0.0	0.0	0.0
64-65	0.0	0.0	0.0	0.0	0.0	0.0
66-67	0.0	0.0	0.0	0.0	0.0	0.0
68-69	0.0	0.0	0.0	0.0	0.0	0.0
70-71	0.0	0.0	0.0	0.0	0.0	0.0
72-73	0.0	0.0	0.0	0.0	0.0	0.0
74-75	0.0	0.0	0.0	0.0	0.0	0.0
76-77	0.0	0.0	0.0	0.0	0.0	0.0
78-79	0.0	0.0	0.0	0.0	0.0	0.0
80-81	0.0	0.0	0.0	0.0	0.0	0.0
82-83	0.0	0.0	0.0	0.0	0.0	0.0
84-85	0.0	0.0	0.0	0.0	0.0	0.0
86-87	0.0	0.0	0.0	0.0	0.0	0.0
88-89	0.0	0.0	0.0	0.0	0.0	0.0
90-91	0.0	0.0	0.0	0.0	0.0	0.0
92-93	0.0	0.0	0.0	0.0	0.0	0.0
94-95	0.0	0.0	0.0	0.0	0.0	0.0
96-97	0.0	0.0	0.0	0.0	0.0	0.0
98-99	0.0	0.0	0.0	0.0	0.0	0.0
100-101	0.0	0.0	0.004	0.0	0.0	0.0
102-103	0.0	0.0	0.004	0.0	0.0	0.0
104-105	0.0	0.0	0.004	0.0	0.0	0.0
106-107	0.0	0.0	0.004	0.0	0.002	0.0
108-109	0.0	0.0	0.004	0.0	0.004	0.0
110-111	0.0	0.0	0.004	0.0	0.004	0.0
112-113	0.0	0.0	0.004	0.0	0.004	0.0
114-115	0.0	0.0	0.004	0.0	0.004	0.0
116-117	0.0	0.0	0.004	0.0	0.004	0.0
118-119	0.0	0.0	0.004	0.0	0.004	0.0
120-121	0.0	0.0	0.004	0.0	0.004	0.0
122-123	0.0	0.0	0.004	0.0	0.004	0.0
124-125	0.0	0.0	0.004	0.0	0.004	0.0
126-127	0.0	0.0	0.004	0.0	0.004	0.0
128-129	0.0	0.0	0.004	0.0	0.004	0.0
130-131	0.0	0.0	0.004	0.0	0.004	0.0
132-133	0.0	0.0	0.004	0.0	0.004	0.0
134-135	0.0	0.0	0.004	0.0	0.004	0.0
136-137	0.0	0.0	0.004	0.0	0.004	0.0
138-139	0.0	0.0	0.004	0.0	0.004	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTAATAC	35	0.0037900615	20.571428	125-129
>>END_MODULE
