##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	S1_GItract_HiSeq_cami_sub25k.R1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	25000
Total Bases	3.7 Mbp
Sequences flagged as poor quality	0
Sequence length	150
%GC	53
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.38176	35.0	32.0	35.0	31.0	35.0
2	33.60272	35.0	32.0	35.0	32.0	35.0
3	33.65324	35.0	32.0	35.0	32.0	35.0
4	37.04688	38.0	36.0	38.0	36.0	38.0
5	36.9864	38.0	36.0	38.0	36.0	38.0
6	36.95724	38.0	36.0	38.0	36.0	38.0
7	36.95564	38.0	36.0	38.0	36.0	38.0
8	36.95136	38.0	36.0	38.0	36.0	38.0
9	38.6378	40.0	38.0	40.0	36.0	40.0
10-14	38.870792	40.2	38.2	40.4	36.0	40.4
15-19	39.888832	41.0	39.0	42.0	35.8	42.0
20-24	39.786744	41.0	39.0	42.0	35.4	42.0
25-29	39.48022400000001	41.0	39.0	42.0	35.0	42.0
30-34	39.120888	41.0	38.8	42.0	34.0	42.0
35-39	38.771831999999996	41.0	37.8	42.0	33.6	42.0
40-44	38.082464	40.2	36.2	42.0	32.2	42.0
45-49	37.340336	39.2	36.0	41.4	31.2	42.0
50-54	36.604608	38.2	35.2	41.0	30.2	42.0
55-59	36.28736	37.0	35.0	40.8	30.2	42.0
60-64	35.726208	36.0	35.0	39.8	30.0	42.0
65-69	35.005736	36.0	34.2	38.2	29.8	41.0
70-74	34.347608	36.0	34.0	37.0	28.4	39.8
75-79	33.69552	35.8	33.8	36.0	27.8	38.0
80-84	33.537664	36.0	34.0	36.0	28.0	37.0
85-89	33.157176	36.0	34.0	36.0	27.4	36.4
90-94	32.897856000000004	36.0	33.8	36.0	26.4	36.0
95-99	32.610056	36.0	33.0	36.0	25.6	36.0
100-104	32.229991999999996	36.0	33.0	36.0	25.0	36.0
105-109	31.92416	35.0	32.0	36.0	23.6	36.0
110-114	31.460272000000003	35.0	32.0	36.0	21.0	36.0
115-119	31.191232000000003	35.0	31.4	36.0	19.8	36.0
120-124	30.851288	35.0	31.0	36.0	17.8	36.0
125-129	30.220528	35.0	30.0	36.0	11.4	36.0
130-134	29.689536000000004	34.8	29.6	36.0	5.0	36.0
135-139	29.092208	34.0	28.0	36.0	3.0	36.0
140-144	28.39124	34.0	27.2	36.0	3.0	36.0
145-149	27.234087999999996	34.0	24.6	36.0	3.6	36.0
150	24.6148	31.0	16.0	35.0	3.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
30	1.0
31	15.0
32	712.0
33	7508.0
34	14242.0
35	2512.0
36	10.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	26.612000000000002	23.276	22.836000000000002	27.276
2	26.967999999999996	22.695999999999998	23.388	26.948
3	27.304000000000002	22.676	23.156	26.863999999999997
4	26.667999999999996	23.212	23.003999999999998	27.116
5	27.332	22.792	22.96	26.916
6	26.928	23.204	23.04	26.828000000000003
7	26.816000000000003	23.355999999999998	22.636	27.192
8	26.44	23.336000000000002	23.119999999999997	27.104
9	26.907999999999998	23.212	23.088	26.791999999999998
10-14	26.9976	22.904	23.1712	26.927200000000003
15-19	27.0496	23.052	23.0104	26.888
20-24	27.211999999999996	22.608800000000002	23.1112	27.067999999999998
25-29	26.904	23.025599999999997	22.999200000000002	27.0712
30-34	27.044	22.9488	23.0048	27.002399999999998
35-39	26.8384	22.932	23.14	27.089600000000004
40-44	26.8384	22.8904	23.1728	27.0984
45-49	26.96	22.825599999999998	23.0872	27.127200000000002
50-54	26.968799999999998	23.2184	22.8	27.0128
55-59	26.928	23.0224	22.944	27.105600000000003
60-64	26.82	22.961599999999997	23.008	27.2104
65-69	26.989600000000003	22.9648	23.1296	26.916
70-74	26.7264	23.1656	22.9688	27.139200000000002
75-79	26.989600000000003	23.0192	23.012	26.9792
80-84	27.1696	23.0464	22.7832	27.0008
85-89	27.001599999999996	23.028000000000002	23.268	26.702399999999997
90-94	27.011200000000002	22.9376	23.0968	26.9544
95-99	26.984799999999996	22.9824	23.1448	26.888
100-104	27.016800000000003	22.8368	23.355999999999998	26.790399999999998
105-109	26.8368	23.1592	23.1752	26.8288
110-114	26.8432	23.052	23.0192	27.0856
115-119	26.757599999999996	23.1784	23.1024	26.9616
120-124	26.767200000000003	23.051199999999998	23.354400000000002	26.8272
125-129	26.7744	23.2288	22.9656	27.0312
130-134	26.999200000000002	22.9928	23.1512	26.856799999999996
135-139	26.874399999999998	23.26	23.247999999999998	26.617600000000003
140-144	26.7472	23.0104	23.124	27.118399999999998
145-149	26.779199999999996	23.2976	23.192	26.7312
150	26.912000000000003	23.608	23.052	26.428
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.5
8	0.5
9	1.0
10	2.0
11	4.0
12	8.0
13	9.5
14	10.5
15	17.5
16	22.0
17	29.5
18	42.5
19	55.0
20	77.0
21	95.0
22	113.0
23	156.0
24	206.0
25	230.0
26	257.0
27	300.0
28	322.5
29	325.5
30	339.0
31	380.0
32	407.5
33	391.5
34	404.0
35	408.5
36	410.5
37	412.5
38	389.0
39	380.0
40	368.0
41	352.0
42	352.5
43	355.0
44	331.0
45	332.5
46	360.5
47	326.0
48	286.5
49	298.0
50	283.0
51	269.0
52	265.0
53	261.0
54	262.5
55	262.5
56	293.5
57	308.5
58	347.5
59	403.0
60	439.0
61	502.0
62	580.5
63	651.0
64	694.5
65	769.5
66	837.5
67	890.0
68	919.0
69	859.0
70	815.5
71	807.0
72	737.0
73	632.5
74	552.0
75	464.5
76	364.0
77	275.0
78	210.0
79	165.5
80	112.0
81	68.0
82	41.5
83	29.5
84	22.5
85	15.5
86	10.0
87	5.5
88	3.0
89	1.5
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
150	25000.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	99.90400000000001
#Duplication Level	Percentage of total
1	99.80799999999999
2	0.192
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
>10	0.0
>50	0.0
>100	0.0
>500	0.0
>1k	0.0
>5k	0.0
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	0.0	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	0.0	0.0	0.0
16-17	0.0	0.0	0.0	0.0	0.0	0.0
18-19	0.0	0.0	0.0	0.0	0.0	0.0
20-21	0.0	0.0	0.0	0.0	0.0	0.0
22-23	0.0	0.0	0.0	0.0	0.0	0.0
24-25	0.0	0.0	0.0	0.0	0.0	0.0
26-27	0.0	0.0	0.0	0.0	0.0	0.0
28-29	0.0	0.0	0.0	0.0	0.0	0.0
30-31	0.0	0.0	0.0	0.0	0.0	0.0
32-33	0.0	0.0	0.0	0.0	0.0	0.0
34-35	0.0	0.0	0.0	0.0	0.0	0.0
36-37	0.0	0.0	0.0	0.0	0.0	0.0
38-39	0.0	0.0	0.0	0.0	0.0	0.0
40-41	0.0	0.0	0.0	0.0	0.0	0.0
42-43	0.0	0.0	0.0	0.0	0.0	0.0
44-45	0.0	0.0	0.0	0.0	0.0	0.0
46-47	0.0	0.0	0.0	0.0	0.0	0.0
48-49	0.0	0.0	0.0	0.0	0.0	0.0
50-51	0.0	0.0	0.0	0.0	0.0	0.0
52-53	0.0	0.0	0.0	0.0	0.0	0.0
54-55	0.0	0.0	0.0	0.0	0.0	0.0
56-57	0.0	0.0	0.0	0.0	0.0	0.0
58-59	0.0	0.0	0.0	0.0	0.0	0.0
60-61	0.0	0.0	0.0	0.0	0.0	0.0
62-63	0.0	0.0	0.0	0.0	0.0	0.0
64-65	0.0	0.0	0.0	0.0	0.0	0.0
66-67	0.0	0.0	0.0	0.0	0.0	0.0
68-69	0.0	0.0	0.0	0.0	0.0	0.0
70-71	0.0	0.0	0.0	0.0	0.0	0.0
72-73	0.0	0.0	0.0	0.0	0.0	0.0
74-75	0.0	0.0	0.0	0.0	0.0	0.0
76-77	0.0	0.0	0.0	0.0	0.0	0.0
78-79	0.0	0.0	0.0	0.0	0.0	0.0
80-81	0.0	0.0	0.0	0.0	0.0	0.0
82-83	0.0	0.0	0.0	0.0	0.0	0.0
84-85	0.0	0.0	0.0	0.0	0.0	0.0
86-87	0.0	0.0	0.0	0.0	0.0	0.0
88-89	0.0	0.0	0.0	0.0	0.0	0.0
90-91	0.0	0.0	0.0	0.0	0.0	0.0
92-93	0.0	0.0	0.0	0.0	0.0	0.0
94-95	0.0	0.0	0.0	0.0	0.0	0.0
96-97	0.0	0.0	0.0	0.0	0.0	0.0
98-99	0.0	0.0	0.0	0.0	0.0	0.0
100-101	0.0	0.0	0.0	0.0	0.0	0.0
102-103	0.0	0.0	0.0	0.0	0.0	0.0
104-105	0.0	0.0	0.0	0.0	0.0	0.0
106-107	0.0	0.0	0.0	0.0	0.0	0.0
108-109	0.0	0.0	0.0	0.0	0.0	0.0
110-111	0.0	0.0	0.0	0.0	0.0	0.0
112-113	0.0	0.0	0.0	0.0	0.0	0.0
114-115	0.0	0.0	0.0	0.0	0.0	0.0
116-117	0.0	0.0	0.0	0.0	0.0	0.0
118-119	0.0	0.0	0.0	0.0	0.0	0.0
120-121	0.0	0.0	0.0	0.0	0.0	0.0
122-123	0.0	0.0	0.0	0.0	0.0	0.0
124-125	0.0	0.0	0.0	0.0	0.0	0.0
126-127	0.0	0.0	0.0	0.0	0.0	0.0
128-129	0.0	0.0	0.0	0.0	0.0	0.0
130-131	0.0	0.0	0.0	0.0	0.0	0.0
132-133	0.0	0.0	0.0	0.0	0.0	0.0
134-135	0.0	0.0	0.0	0.0	0.0	0.0
136-137	0.0	0.0	0.0	0.0	0.0	0.0
138-139	0.0	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCACAAT	10	0.0071972376	144.0	1
CAGTTTC	25	9.537935E-4	86.399994	6
CCAGTTT	30	0.0019669293	72.0	5
>>END_MODULE
