##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	S1_GItract_HiSeq_cami_sub25k.R2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	25000
Total Bases	3.7 Mbp
Sequences flagged as poor quality	0
Sequence length	150
%GC	53
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.12488	34.0	32.0	35.0	29.0	35.0
2	32.24328	35.0	32.0	35.0	29.0	35.0
3	32.31156	35.0	32.0	35.0	29.0	35.0
4	35.79472	38.0	36.0	38.0	33.0	38.0
5	35.84328	38.0	36.0	38.0	33.0	38.0
6	35.74844	38.0	36.0	38.0	33.0	38.0
7	35.77112	38.0	36.0	38.0	33.0	38.0
8	35.67048	38.0	36.0	38.0	33.0	38.0
9	37.34256	40.0	38.0	40.0	33.0	40.0
10-14	37.543688	40.2	38.2	40.4	33.0	40.4
15-19	38.510360000000006	41.0	39.0	42.0	33.0	42.0
20-24	38.400712	41.0	39.0	42.0	32.4	42.0
25-29	38.00816	41.0	38.0	42.0	31.4	42.0
30-34	37.632984	41.0	37.6	42.0	31.0	42.0
35-39	37.252559999999995	40.8	36.4	42.0	30.4	42.0
40-44	36.58855200000001	39.8	36.0	42.0	28.6	42.0
45-49	35.846199999999996	38.8	35.0	41.2	27.2	42.0
50-54	34.892432	37.2	34.2	40.8	25.4	41.8
55-59	34.373104	36.2	34.0	40.4	24.0	42.0
60-64	33.555552	36.0	32.8	39.4	23.0	41.4
65-69	32.743504	36.0	32.0	37.6	21.2	40.6
70-74	32.685288	36.0	33.0	37.0	22.4	39.8
75-79	32.307472000000004	36.0	33.0	36.0	21.2	38.0
80-84	31.762808	36.0	32.6	36.0	20.0	37.0
85-89	31.278016	35.8	32.0	36.0	18.0	36.2
90-94	30.940143999999997	35.0	32.0	36.0	13.8	36.0
95-99	30.665816	35.0	31.0	36.0	8.6	36.0
100-104	30.264488	35.0	30.8	36.0	4.2	36.0
105-109	29.85756	35.0	30.0	36.0	3.0	36.0
110-114	29.500456000000003	35.0	30.0	36.0	3.0	36.0
115-119	29.078408000000003	35.0	28.0	36.0	3.0	36.0
120-124	28.60256	34.0	27.6	36.0	3.0	36.0
125-129	27.979167999999998	34.0	25.6	36.0	3.0	36.0
130-134	27.366824	34.0	24.8	36.0	3.0	36.0
135-139	26.729696	33.6	22.8	36.0	3.0	36.0
140-144	25.789640000000002	33.0	16.8	36.0	3.0	36.0
145-149	24.326880000000003	32.0	3.0	35.0	3.0	36.0
150	22.58952	30.0	3.0	35.0	3.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
28	5.0
29	84.0
30	1154.0
31	6354.0
32	11632.0
33	5365.0
34	406.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	26.772000000000002	23.028000000000002	23.028000000000002	27.172
2	26.676	23.427999999999997	23.24	26.656000000000002
3	26.984	22.747999999999998	23.216	27.052
4	27.288	23.072	22.747999999999998	26.892
5	27.128000000000004	23.183999999999997	22.944	26.744
6	26.928	23.355999999999998	22.856	26.86
7	27.112000000000002	23.3	22.7	26.888
8	27.004	23.568	22.476	26.951999999999998
9	26.612000000000002	22.688	23.268	27.432000000000002
10-14	27.096799999999998	23.0024	23.000799999999998	26.900000000000002
15-19	26.996	23.0368	23.0072	26.96
20-24	26.927200000000003	22.844	23.1856	27.0432
25-29	27.084000000000003	23.169600000000003	22.9456	26.800800000000002
30-34	26.9872	23.0928	22.948	26.972
35-39	26.7776	22.9544	23.2232	27.044800000000002
40-44	27.0272	23.1568	22.9584	26.857599999999998
45-49	27.0176	23.119999999999997	22.9544	26.907999999999998
50-54	26.879199999999997	23.1	23.119999999999997	26.900800000000004
55-59	26.76	23.3	23.1464	26.7936
60-64	27.023999999999997	23.2272	22.933600000000002	26.8152
65-69	26.884000000000004	22.916800000000002	23.116	27.0832
70-74	26.746399999999998	23.0392	23.285600000000002	26.928800000000003
75-79	26.946399999999997	23.112	23.2024	26.7392
80-84	27.095200000000002	23.0312	23.0576	26.816000000000003
85-89	27.007199999999997	23.088800000000003	23.1176	26.7864
90-94	27.023999999999997	22.825599999999998	23.231199999999998	26.9192
95-99	27.0224	23.285600000000002	22.844	26.848
100-104	26.8808	23.0432	23.0328	27.0432
105-109	26.934399999999997	23.0744	23.2208	26.7704
110-114	26.840000000000003	23.2848	23.1856	26.689600000000002
115-119	26.7816	23.145599999999998	23.3272	26.745600000000003
120-124	26.817600000000002	23.2472	23.2224	26.712799999999998
125-129	26.9152	23.1184	23.3264	26.640000000000004
130-134	26.7696	23.368	23.3352	26.5272
135-139	26.604	23.604	23.1648	26.627200000000002
140-144	26.9264	23.3368	22.9832	26.7536
145-149	26.7656	23.2864	23.4768	26.4712
150	26.296000000000003	23.552	23.276	26.876
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.5
11	1.0
12	0.5
13	4.0
14	9.0
15	16.0
16	19.5
17	28.5
18	47.0
19	59.5
20	66.0
21	74.5
22	109.5
23	145.0
24	173.0
25	202.0
26	224.0
27	276.5
28	291.5
29	297.5
30	354.5
31	395.5
32	407.5
33	409.0
34	415.5
35	419.0
36	410.0
37	396.5
38	405.0
39	412.5
40	391.0
41	389.5
42	381.0
43	351.0
44	339.0
45	337.5
46	336.5
47	328.5
48	302.0
49	287.0
50	285.5
51	272.5
52	273.5
53	276.0
54	274.0
55	285.0
56	316.0
57	344.5
58	380.5
59	420.5
60	479.0
61	559.5
62	629.0
63	705.5
64	774.0
65	830.5
66	832.5
67	871.5
68	918.5
69	885.0
70	818.0
71	737.5
72	688.5
73	617.5
74	523.5
75	413.0
76	310.5
77	231.5
78	173.5
79	124.0
80	78.0
81	59.0
82	40.5
83	23.0
84	17.0
85	9.5
86	4.0
87	3.0
88	1.0
89	1.0
90	0.5
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
150	25000.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	99.968
#Duplication Level	Percentage of total
1	99.936
2	0.064
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
>10	0.0
>50	0.0
>100	0.0
>500	0.0
>1k	0.0
>5k	0.0
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	0.0	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	0.0	0.0	0.0
16-17	0.0	0.0	0.0	0.0	0.0	0.0
18-19	0.0	0.0	0.0	0.0	0.0	0.0
20-21	0.0	0.0	0.0	0.0	0.0	0.0
22-23	0.0	0.0	0.0	0.0	0.0	0.0
24-25	0.0	0.0	0.0	0.0	0.0	0.0
26-27	0.0	0.0	0.0	0.0	0.0	0.0
28-29	0.0	0.0	0.0	0.0	0.0	0.0
30-31	0.0	0.0	0.0	0.0	0.0	0.0
32-33	0.0	0.0	0.0	0.0	0.0	0.0
34-35	0.0	0.0	0.0	0.0	0.0	0.0
36-37	0.0	0.0	0.0	0.0	0.0	0.0
38-39	0.0	0.0	0.0	0.0	0.0	0.0
40-41	0.0	0.0	0.0	0.0	0.0	0.0
42-43	0.0	0.0	0.0	0.0	0.0	0.0
44-45	0.0	0.0	0.0	0.0	0.0	0.0
46-47	0.0	0.0	0.0	0.0	0.0	0.0
48-49	0.0	0.0	0.0	0.0	0.0	0.0
50-51	0.0	0.0	0.0	0.0	0.0	0.0
52-53	0.0	0.0	0.0	0.0	0.0	0.0
54-55	0.0	0.0	0.0	0.0	0.0	0.0
56-57	0.0	0.0	0.0	0.0	0.0	0.0
58-59	0.0	0.0	0.0	0.0	0.0	0.0
60-61	0.0	0.0	0.0	0.004	0.0	0.0
62-63	0.0	0.0	0.0	0.004	0.0	0.0
64-65	0.0	0.0	0.0	0.004	0.0	0.0
66-67	0.0	0.0	0.0	0.004	0.0	0.0
68-69	0.0	0.0	0.0	0.004	0.0	0.0
70-71	0.0	0.0	0.0	0.004	0.0	0.0
72-73	0.0	0.0	0.0	0.004	0.0	0.0
74-75	0.0	0.0	0.0	0.004	0.0	0.0
76-77	0.0	0.0	0.0	0.004	0.0	0.0
78-79	0.0	0.0	0.0	0.004	0.0	0.0
80-81	0.0	0.0	0.0	0.004	0.0	0.0
82-83	0.0	0.0	0.0	0.004	0.0	0.0
84-85	0.0	0.0	0.0	0.004	0.0	0.0
86-87	0.0	0.0	0.0	0.004	0.0	0.0
88-89	0.0	0.0	0.0	0.004	0.0	0.0
90-91	0.0	0.0	0.0	0.004	0.0	0.0
92-93	0.0	0.0	0.0	0.004	0.0	0.0
94-95	0.0	0.0	0.0	0.004	0.002	0.0
96-97	0.0	0.0	0.0	0.004	0.004	0.0
98-99	0.0	0.0	0.0	0.004	0.004	0.0
100-101	0.0	0.0	0.0	0.004	0.004	0.0
102-103	0.0	0.0	0.0	0.004	0.004	0.0
104-105	0.0	0.0	0.0	0.004	0.004	0.0
106-107	0.0	0.0	0.0	0.004	0.004	0.0
108-109	0.0	0.0	0.0	0.004	0.008	0.0
110-111	0.0	0.0	0.0	0.004	0.008	0.0
112-113	0.0	0.0	0.0	0.004	0.008	0.0
114-115	0.0	0.0	0.0	0.004	0.008	0.0
116-117	0.0	0.0	0.0	0.004	0.008	0.0
118-119	0.0	0.0	0.0	0.004	0.008	0.0
120-121	0.0	0.0	0.0	0.004	0.008	0.0
122-123	0.0	0.0	0.0	0.004	0.008	0.0
124-125	0.0	0.0	0.0	0.004	0.008	0.0
126-127	0.0	0.0	0.0	0.004	0.008	0.0
128-129	0.0	0.0	0.0	0.004	0.008	0.0
130-131	0.0	0.0	0.0	0.004	0.008	0.0
132-133	0.0	0.0	0.0	0.004	0.008	0.0
134-135	0.0	0.0	0.0	0.004	0.008	0.0
136-137	0.0	0.0	0.0	0.004	0.008	0.0
138-139	0.0	0.0	0.0	0.004	0.008	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGTAAA	10	0.0071972376	144.0	3
CGATACG	10	0.0071972376	144.0	3
>>END_MODULE
