##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	S6_Oral_HiSeq_cami_sub25k.R2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	25000
Total Bases	3.7 Mbp
Sequences flagged as poor quality	0
Sequence length	150
%GC	41
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.15536	34.0	32.0	35.0	29.0	35.0
2	32.27328	34.0	32.0	35.0	29.0	35.0
3	32.3122	35.0	32.0	35.0	29.0	35.0
4	35.76316	38.0	36.0	38.0	33.0	38.0
5	35.79096	38.0	36.0	38.0	33.0	38.0
6	35.79136	38.0	36.0	38.0	33.0	38.0
7	35.75624	38.0	36.0	38.0	33.0	38.0
8	35.75672	38.0	36.0	38.0	33.0	38.0
9	37.29624	40.0	38.0	40.0	33.0	40.0
10-14	37.568336	40.2	38.2	40.4	33.0	40.4
15-19	38.517343999999994	41.0	39.0	42.0	33.0	42.0
20-24	38.406512	41.0	39.0	42.0	32.6	42.0
25-29	38.029072	41.0	38.0	42.0	31.6	42.0
30-34	37.643184	41.0	37.4	42.0	31.0	42.0
35-39	37.174192	40.6	36.4	42.0	30.0	42.0
40-44	36.625375999999996	39.8	36.0	42.0	28.6	42.0
45-49	35.81013599999999	38.8	35.0	41.2	27.2	42.0
50-54	34.905184	37.2	34.0	40.8	25.2	41.8
55-59	34.381936	36.2	34.0	40.4	24.0	42.0
60-64	33.533	36.0	32.8	39.2	23.0	41.6
65-69	32.713352	36.0	32.0	37.6	21.0	40.6
70-74	32.725824	36.0	33.0	36.8	22.6	40.0
75-79	32.30870399999999	36.0	33.0	36.0	21.8	38.0
80-84	31.752968	36.0	32.8	36.0	19.6	37.0
85-89	31.303727999999996	35.8	32.0	36.0	18.2	36.4
90-94	30.948528000000003	35.0	31.6	36.0	14.4	36.0
95-99	30.659744	35.0	31.0	36.0	9.2	36.0
100-104	30.354480000000002	35.0	30.8	36.0	5.6	36.0
105-109	29.882896	35.0	30.0	36.0	3.0	36.0
110-114	29.542032	35.0	30.0	36.0	3.0	36.0
115-119	29.049135999999997	35.0	28.4	36.0	3.0	36.0
120-124	28.625040000000002	34.0	27.6	36.0	3.0	36.0
125-129	28.003839999999997	34.0	25.8	36.0	3.0	36.0
130-134	27.382888	34.0	25.0	36.0	3.0	36.0
135-139	26.684255999999998	33.4	22.2	36.0	3.0	36.0
140-144	25.713839999999998	33.0	16.8	36.0	3.0	36.0
145-149	24.478631999999998	32.2	3.0	35.0	3.0	36.0
150	22.58964	30.0	3.0	35.0	3.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
28	3.0
29	76.0
30	1086.0
31	6360.0
32	11637.0
33	5396.0
34	440.0
35	2.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	19.948	29.364	29.843999999999998	20.843999999999998
2	20.288	29.132	29.872	20.708
3	20.348	29.487999999999996	29.476000000000003	20.688000000000002
4	20.552	29.580000000000002	29.343999999999998	20.524
5	20.524	29.752000000000002	29.224	20.5
6	20.416	29.812	29.304000000000002	20.468
7	20.46	29.536	29.768	20.236
8	20.396	29.496	29.568	20.54
9	19.759999999999998	30.556	29.724	19.96
10-14	20.560000000000002	29.399199999999997	29.4672	20.5736
15-19	20.4104	29.836800000000004	29.193599999999996	20.5592
20-24	20.556	29.592000000000002	29.304000000000002	20.548
25-29	20.5328	29.4144	29.3736	20.6792
30-34	20.4688	29.6464	29.348800000000004	20.535999999999998
35-39	20.488	29.5512	29.404799999999998	20.556
40-44	20.5048	29.386400000000002	29.541600000000003	20.5672
45-49	20.7936	29.182399999999998	29.316799999999997	20.7072
50-54	20.768800000000002	29.243999999999996	29.406399999999998	20.5808
55-59	20.6832	29.4528	29.171200000000002	20.6928
60-64	20.436	29.237600000000004	29.6352	20.691200000000002
65-69	20.6112	29.2352	29.3184	20.8352
70-74	20.6488	29.54	29.1512	20.66
75-79	20.5944	29.2016	29.4072	20.796799999999998
80-84	20.7744	29.1856	29.2816	20.758399999999998
85-89	20.6888	29.3088	29.2992	20.7032
90-94	20.719199999999997	29.0864	29.425600000000003	20.768800000000002
95-99	20.8192	29.162399999999998	29.2504	20.768
100-104	20.9144	29.227199999999996	29.1312	20.7272
105-109	20.6224	29.3904	29.1528	20.8344
110-114	20.8752	29.413600000000002	28.9024	20.808799999999998
115-119	20.918400000000002	29.016	29.343200000000003	20.7224
120-124	20.855999999999998	29.1528	29.136	20.8552
125-129	20.8368	29.017599999999998	29.036800000000003	21.1088
130-134	20.8512	28.9864	29.186400000000003	20.976
135-139	20.8768	28.777599999999996	29.242400000000004	21.1032
140-144	21.4848	28.886400000000002	28.539199999999997	21.0896
145-149	21.438399999999998	28.729599999999998	28.4256	21.4064
150	21.372	28.323999999999998	28.336	21.968
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.5
7	0.5
8	0.0
9	0.0
10	0.0
11	0.0
12	1.5
13	3.0
14	3.5
15	5.0
16	7.0
17	11.0
18	17.0
19	20.5
20	31.5
21	45.5
22	66.0
23	92.5
24	126.0
25	161.5
26	194.5
27	244.0
28	298.5
29	359.0
30	430.0
31	501.5
32	600.0
33	726.0
34	806.0
35	893.0
36	1003.0
37	1067.0
38	1165.0
39	1294.5
40	1342.0
41	1289.5
42	1277.0
43	1245.0
44	1112.5
45	1026.5
46	954.5
47	878.0
48	780.0
49	651.5
50	584.5
51	540.0
52	478.0
53	405.0
54	346.5
55	300.5
56	269.5
57	239.5
58	197.0
59	162.5
60	140.5
61	123.0
62	99.5
63	80.5
64	66.5
65	55.5
66	41.5
67	32.0
68	23.0
69	16.5
70	16.5
71	15.0
72	10.0
73	7.5
74	5.5
75	3.5
76	3.5
77	2.5
78	1.5
79	1.0
80	0.0
81	0.5
82	0.5
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
150	25000.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	99.996
#Duplication Level	Percentage of total
1	99.992
2	0.008
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
>10	0.0
>50	0.0
>100	0.0
>500	0.0
>1k	0.0
>5k	0.0
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	0.0	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	0.0	0.0	0.0
16-17	0.0	0.0	0.0	0.0	0.0	0.0
18-19	0.0	0.0	0.0	0.0	0.0	0.0
20-21	0.0	0.0	0.0	0.0	0.0	0.0
22-23	0.0	0.0	0.0	0.0	0.0	0.0
24-25	0.0	0.0	0.0	0.0	0.0	0.0
26-27	0.0	0.0	0.0	0.0	0.0	0.0
28-29	0.0	0.0	0.0	0.0	0.002	0.0
30-31	0.0	0.0	0.0	0.0	0.004	0.0
32-33	0.0	0.0	0.0	0.0	0.004	0.0
34-35	0.0	0.0	0.0	0.0	0.004	0.0
36-37	0.0	0.0	0.0	0.0	0.004	0.0
38-39	0.0	0.0	0.0	0.0	0.004	0.0
40-41	0.0	0.0	0.0	0.0	0.004	0.0
42-43	0.0	0.0	0.0	0.0	0.004	0.0
44-45	0.0	0.0	0.0	0.0	0.004	0.0
46-47	0.0	0.0	0.0	0.0	0.004	0.0
48-49	0.0	0.0	0.0	0.0	0.004	0.0
50-51	0.0	0.0	0.0	0.0	0.004	0.0
52-53	0.0	0.0	0.0	0.0	0.004	0.0
54-55	0.0	0.0	0.0	0.0	0.004	0.0
56-57	0.0	0.0	0.0	0.0	0.004	0.0
58-59	0.0	0.0	0.0	0.0	0.004	0.0
60-61	0.0	0.0	0.0	0.0	0.004	0.0
62-63	0.0	0.0	0.0	0.0	0.004	0.0
64-65	0.0	0.0	0.0	0.004	0.004	0.0
66-67	0.0	0.0	0.0	0.004	0.004	0.0
68-69	0.0	0.0	0.0	0.004	0.004	0.0
70-71	0.0	0.0	0.0	0.004	0.004	0.0
72-73	0.0	0.0	0.0	0.004	0.004	0.0
74-75	0.0	0.0	0.0	0.004	0.004	0.0
76-77	0.0	0.0	0.0	0.004	0.004	0.0
78-79	0.0	0.0	0.0	0.004	0.004	0.0
80-81	0.0	0.0	0.0	0.004	0.004	0.0
82-83	0.0	0.0	0.0	0.004	0.004	0.0
84-85	0.0	0.0	0.0	0.004	0.004	0.0
86-87	0.0	0.0	0.0	0.004	0.004	0.0
88-89	0.0	0.0	0.0	0.004	0.004	0.0
90-91	0.0	0.0	0.0	0.004	0.004	0.0
92-93	0.0	0.0	0.0	0.004	0.004	0.0
94-95	0.0	0.0	0.0	0.004	0.004	0.0
96-97	0.0	0.0	0.0	0.004	0.004	0.0
98-99	0.0	0.0	0.0	0.004	0.004	0.0
100-101	0.0	0.0	0.0	0.004	0.004	0.0
102-103	0.0	0.0	0.0	0.004	0.004	0.0
104-105	0.0	0.0	0.0	0.004	0.004	0.0
106-107	0.0	0.0	0.0	0.004	0.004	0.0
108-109	0.0	0.0	0.0	0.004	0.004	0.0
110-111	0.0	0.0	0.0	0.004	0.004	0.0
112-113	0.0	0.0	0.0	0.004	0.004	0.0
114-115	0.0	0.0	0.0	0.004	0.004	0.0
116-117	0.0	0.0	0.0	0.004	0.004	0.0
118-119	0.0	0.0	0.0	0.004	0.004	0.0
120-121	0.0	0.0	0.0	0.004	0.004	0.0
122-123	0.0	0.0	0.0	0.004	0.004	0.0
124-125	0.0	0.0	0.0	0.004	0.004	0.0
126-127	0.0	0.0	0.0	0.004	0.004	0.0
128-129	0.0	0.0	0.0	0.004	0.004	0.0
130-131	0.0	0.0	0.0	0.004	0.004	0.0
132-133	0.0	0.0	0.0	0.004	0.004	0.0
134-135	0.0	0.0	0.0	0.004	0.004	0.0
136-137	0.0	0.0	0.0	0.004	0.004	0.0
138-139	0.0	0.0	0.0	0.004	0.004	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGGTCGT	10	0.0071972376	144.0	4
>>END_MODULE
