##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	S9_Air_HiSeq_cami_sub25k.R2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	25000
Total Bases	3.7 Mbp
Sequences flagged as poor quality	0
Sequence length	150
%GC	47
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.13192	34.0	32.0	35.0	29.0	35.0
2	32.23836	35.0	32.0	35.0	29.0	35.0
3	32.28288	35.0	32.0	35.0	29.0	35.0
4	35.76976	38.0	36.0	38.0	33.0	38.0
5	35.7748	38.0	36.0	38.0	33.0	38.0
6	35.76936	38.0	36.0	38.0	33.0	38.0
7	35.76932	38.0	36.0	38.0	33.0	38.0
8	35.7172	38.0	36.0	38.0	33.0	38.0
9	37.4112	40.0	38.0	40.0	33.0	40.0
10-14	37.527816	40.2	38.2	40.4	33.0	40.4
15-19	38.510744	41.0	39.0	42.0	33.0	42.0
20-24	38.378712	41.0	39.0	42.0	32.6	42.0
25-29	38.022071999999994	41.0	38.0	42.0	31.4	42.0
30-34	37.651112	41.0	37.6	42.0	31.0	42.0
35-39	37.279128	40.6	36.6	42.0	30.4	42.0
40-44	36.628776	39.8	36.0	42.0	28.6	42.0
45-49	35.884823999999995	38.8	35.2	41.2	27.0	42.0
50-54	34.903704000000005	37.2	34.0	40.8	25.4	41.8
55-59	34.358064000000006	36.2	34.0	40.4	24.0	42.0
60-64	33.606719999999996	36.0	33.2	39.4	23.2	41.4
65-69	32.742576	36.0	32.0	37.6	21.2	40.6
70-74	32.68336000000001	36.0	33.0	36.8	22.2	39.8
75-79	32.28736	36.0	33.0	36.0	21.4	38.0
80-84	31.77212	36.0	32.6	36.0	20.2	37.0
85-89	31.254944000000002	35.8	32.0	36.0	17.2	36.2
90-94	30.919864	35.0	32.0	36.0	13.4	36.0
95-99	30.626112	35.0	31.2	36.0	9.0	36.0
100-104	30.328512	35.0	30.8	36.0	5.2	36.0
105-109	29.858096	35.0	30.0	36.0	3.0	36.0
110-114	29.457416000000002	35.0	29.2	36.0	3.0	36.0
115-119	29.0976	35.0	28.0	36.0	3.0	36.0
120-124	28.580752	34.2	27.4	36.0	3.0	36.0
125-129	28.028768000000003	34.0	25.8	36.0	3.0	36.0
130-134	27.328832	34.0	24.8	36.0	3.0	36.0
135-139	26.677496	33.6	22.0	36.0	3.0	36.0
140-144	25.765576	33.0	16.6	36.0	3.0	36.0
145-149	24.453424000000002	32.0	3.6	35.2	3.0	36.0
150	22.45968	30.0	3.0	35.0	3.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
28	3.0
29	89.0
30	1178.0
31	6292.0
32	11665.0
33	5361.0
34	411.0
35	1.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	23.872	25.86	26.404	23.864
2	23.604	26.548	26.444000000000003	23.404
3	23.472	26.607999999999997	26.628	23.291999999999998
4	24.288	26.06	26.148	23.504
5	23.28	26.996	26.476	23.247999999999998
6	23.555999999999997	26.856	26.200000000000003	23.388
7	23.483999999999998	26.223999999999997	26.672	23.62
8	22.992	26.32	26.895999999999997	23.791999999999998
9	24.043999999999997	26.06	26.304	23.592
10-14	23.534399999999998	26.317600000000002	26.5368	23.6112
15-19	23.5952	26.535999999999998	26.1864	23.6824
20-24	23.619200000000003	26.4984	26.2536	23.6288
25-29	23.6824	26.0704	26.422400000000003	23.8248
30-34	23.4784	26.618399999999998	26.135199999999998	23.768
35-39	23.4832	26.4032	26.462400000000002	23.6512
40-44	23.4488	26.3392	26.3544	23.8576
45-49	23.4384	26.361600000000003	26.460800000000003	23.7392
50-54	23.3744	26.4976	26.4936	23.6344
55-59	23.4744	26.475199999999997	26.441599999999998	23.6088
60-64	23.6488	26.36	26.3728	23.6184
65-69	23.7904	26.4576	26.112800000000004	23.6392
70-74	23.8192	26.528000000000002	26.128800000000002	23.524
75-79	23.552	26.351200000000002	26.4488	23.648
80-84	23.6824	26.2104	26.5544	23.552799999999998
85-89	23.576800000000002	26.439200000000003	26.4104	23.5736
90-94	23.409174546792748	26.350821613145808	26.498023968383492	23.741979871677945
95-99	23.3944	26.383200000000002	26.4384	23.784
100-104	23.448987591900735	26.402611220889767	26.11860894887159	24.029792238337908
105-109	23.6544	26.3488	26.328000000000003	23.6688
110-114	23.627200000000002	26.214399999999998	26.300800000000002	23.8576
115-119	23.9136	26.228800000000003	26.276	23.581599999999998
120-124	23.593600000000002	26.3	26.291199999999996	23.8152
125-129	23.764	26.2768	26.233600000000003	23.7256
130-134	23.732	26.504	25.9488	23.8152
135-139	23.627200000000002	26.1936	26.204	23.9752
140-144	23.7256	26.2368	26.163999999999998	23.8736
145-149	23.820800000000002	25.9904	26.3264	23.8624
150	23.496	26.351999999999997	26.532	23.62
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.5
14	2.0
15	2.0
16	2.5
17	6.5
18	13.0
19	19.5
20	31.5
21	48.0
22	62.0
23	90.5
24	132.5
25	175.0
26	212.0
27	247.0
28	317.5
29	400.5
30	458.0
31	481.5
32	535.5
33	586.5
34	602.0
35	600.0
36	602.0
37	596.5
38	560.5
39	529.5
40	489.0
41	458.5
42	444.0
43	435.0
44	432.5
45	433.0
46	465.5
47	534.0
48	578.5
49	641.5
50	736.0
51	812.5
52	873.0
53	938.5
54	983.5
55	972.5
56	944.5
57	942.5
58	885.0
59	788.5
60	700.0
61	575.5
62	469.5
63	406.5
64	325.0
65	264.5
66	235.5
67	177.0
68	147.5
69	131.0
70	99.0
71	78.0
72	70.5
73	66.5
74	49.0
75	30.0
76	21.0
77	15.5
78	10.5
79	7.5
80	4.5
81	5.0
82	4.0
83	2.0
84	0.5
85	0.0
86	0.0
87	0.5
88	0.5
89	0.0
90	0.0
91	0.5
92	0.5
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0015999999999999999
95-99	0.0
100-104	7.999999999999999E-4
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
150	25000.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	99.988
#Duplication Level	Percentage of total
1	99.976
2	0.024
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
>10	0.0
>50	0.0
>100	0.0
>500	0.0
>1k	0.0
>5k	0.0
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	0.0	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	0.0	0.0	0.0
16-17	0.0	0.0	0.0	0.0	0.0	0.0
18-19	0.0	0.0	0.0	0.0	0.0	0.0
20-21	0.0	0.0	0.0	0.0	0.0	0.0
22-23	0.0	0.0	0.0	0.0	0.0	0.0
24-25	0.0	0.0	0.0	0.0	0.0	0.0
26-27	0.0	0.0	0.0	0.0	0.0	0.0
28-29	0.0	0.0	0.0	0.0	0.0	0.0
30-31	0.0	0.0	0.0	0.0	0.0	0.0
32-33	0.0	0.0	0.0	0.0	0.0	0.0
34-35	0.0	0.0	0.0	0.0	0.0	0.0
36-37	0.0	0.0	0.0	0.0	0.0	0.0
38-39	0.0	0.0	0.0	0.0	0.0	0.0
40-41	0.0	0.0	0.0	0.0	0.0	0.0
42-43	0.0	0.0	0.004	0.0	0.0	0.0
44-45	0.0	0.0	0.004	0.0	0.0	0.0
46-47	0.0	0.0	0.004	0.0	0.0	0.0
48-49	0.0	0.0	0.004	0.0	0.0	0.0
50-51	0.0	0.0	0.004	0.0	0.0	0.0
52-53	0.0	0.0	0.004	0.0	0.0	0.0
54-55	0.0	0.0	0.004	0.0	0.0	0.0
56-57	0.0	0.0	0.004	0.0	0.0	0.0
58-59	0.0	0.0	0.004	0.0	0.0	0.0
60-61	0.0	0.0	0.004	0.0	0.0	0.0
62-63	0.0	0.0	0.004	0.0	0.0	0.0
64-65	0.0	0.0	0.004	0.0	0.0	0.0
66-67	0.0	0.0	0.004	0.0	0.0	0.0
68-69	0.0	0.0	0.004	0.0	0.0	0.0
70-71	0.0	0.0	0.004	0.0	0.0	0.0
72-73	0.0	0.0	0.004	0.0	0.0	0.0
74-75	0.0	0.0	0.004	0.0	0.0	0.0
76-77	0.0	0.0	0.004	0.0	0.0	0.0
78-79	0.0	0.0	0.004	0.0	0.0	0.0
80-81	0.0	0.0	0.004	0.0	0.0	0.0
82-83	0.0	0.0	0.004	0.0	0.0	0.0
84-85	0.0	0.0	0.004	0.0	0.0	0.0
86-87	0.0	0.0	0.004	0.0	0.0	0.0
88-89	0.0	0.0	0.004	0.0	0.0	0.0
90-91	0.0	0.0	0.004	0.0	0.0	0.0
92-93	0.0	0.0	0.004	0.0	0.0	0.0
94-95	0.0	0.0	0.004	0.0	0.0	0.0
96-97	0.0	0.0	0.004	0.0	0.0	0.0
98-99	0.0	0.0	0.004	0.0	0.0	0.0
100-101	0.0	0.0	0.004	0.0	0.0	0.0
102-103	0.0	0.0	0.004	0.0	0.0	0.0
104-105	0.0	0.0	0.004	0.0	0.0	0.0
106-107	0.0	0.0	0.004	0.0	0.0	0.0
108-109	0.0	0.0	0.004	0.0	0.0	0.0
110-111	0.0	0.0	0.004	0.0	0.0	0.0
112-113	0.0	0.0	0.004	0.0	0.0	0.0
114-115	0.0	0.0	0.004	0.0	0.0	0.0
116-117	0.0	0.0	0.004	0.0	0.0	0.0
118-119	0.0	0.0	0.006	0.0	0.0	0.0
120-121	0.0	0.0	0.008	0.0	0.0	0.0
122-123	0.0	0.0	0.008	0.0	0.0	0.0
124-125	0.0	0.0	0.008	0.0	0.0	0.0
126-127	0.0	0.0	0.008	0.0	0.0	0.0
128-129	0.0	0.0	0.008	0.0	0.0	0.0
130-131	0.0	0.0	0.008	0.0	0.0	0.0
132-133	0.0	0.0	0.008	0.0	0.0	0.0
134-135	0.0	0.0	0.008	0.0	0.0	0.0
136-137	0.0	0.0	0.008	0.0	0.0	0.0
138-139	0.0	0.0	0.008	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTGTCTG	35	0.00362398	61.71429	8
AAATCCA	45	0.009794569	48.0	3
>>END_MODULE
