##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	S28_Skin_HiSeq_cami_sub25k.R1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	25000
Total Bases	3.7 Mbp
Sequences flagged as poor quality	0
Sequence length	150
%GC	40
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.41724	35.0	32.0	35.0	32.0	35.0
2	33.61424	35.0	32.0	35.0	32.0	35.0
3	33.64284	35.0	32.0	35.0	32.0	35.0
4	37.0682	38.0	36.0	38.0	36.0	38.0
5	36.9446	38.0	36.0	38.0	36.0	38.0
6	36.95768	38.0	36.0	38.0	36.0	38.0
7	36.96268	38.0	36.0	38.0	36.0	38.0
8	36.94908	38.0	36.0	38.0	36.0	38.0
9	38.66368	40.0	38.0	40.0	36.0	40.0
10-14	38.874808	40.2	38.2	40.4	36.0	40.4
15-19	39.913064	41.0	39.0	42.0	36.0	42.0
20-24	39.766287999999996	41.0	39.0	42.0	35.2	42.0
25-29	39.470600000000005	41.0	39.0	42.0	34.8	42.0
30-34	39.13879999999999	41.0	38.6	42.0	34.0	42.0
35-39	38.745144	41.0	37.8	42.0	33.4	42.0
40-44	38.05732	40.2	36.2	42.0	32.2	42.0
45-49	37.328255999999996	39.2	36.0	41.4	31.2	42.0
50-54	36.657344	38.2	35.0	41.0	30.2	42.0
55-59	36.284912000000006	37.0	35.0	40.8	30.4	42.0
60-64	35.683	36.0	35.0	39.8	30.0	42.0
65-69	35.0116	36.0	34.2	38.2	29.6	41.0
70-74	34.310224000000005	36.0	34.0	36.8	28.2	39.8
75-79	33.735328	35.8	33.8	36.0	27.8	38.0
80-84	33.53629600000001	36.0	34.0	36.0	28.0	37.0
85-89	33.172936	36.0	34.0	36.0	27.6	36.6
90-94	32.844032	36.0	33.6	36.0	26.0	36.0
95-99	32.584512	36.0	33.0	36.0	25.8	36.0
100-104	32.239951999999995	36.0	32.8	36.0	25.0	36.0
105-109	31.875912	35.0	32.0	36.0	23.2	36.0
110-114	31.466503999999997	35.0	32.0	36.0	20.8	36.0
115-119	31.169712000000004	35.0	31.2	36.0	19.8	36.0
120-124	30.888455999999998	35.0	31.0	36.0	18.4	36.0
125-129	30.250192000000006	35.0	30.0	36.0	11.4	36.0
130-134	29.673544	35.0	30.0	36.0	4.4	36.0
135-139	29.122536000000004	34.0	28.0	36.0	3.0	36.0
140-144	28.38084	34.0	27.2	36.0	3.0	36.0
145-149	27.235144000000002	34.0	24.6	36.0	3.6	36.0
150	24.7022	31.0	16.0	35.0	3.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
31	15.0
32	718.0
33	7517.0
34	14253.0
35	2484.0
36	13.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	20.588	29.587999999999997	29.412	20.412
2	19.924	30.312	29.884	19.88
3	20.612	29.736	30.020000000000003	19.631999999999998
4	20.464	29.415999999999997	29.82	20.3
5	20.448	29.332	30.14	20.080000000000002
6	20.564	29.220000000000002	30.076000000000004	20.14
7	20.384	29.724	29.096	20.796
8	20.4	29.764000000000003	29.804000000000002	20.032
9	19.944	30.192000000000004	29.720000000000002	20.144000000000002
10-14	20.247999999999998	29.7864	29.704000000000004	20.261599999999998
15-19	20.268800000000002	29.828	29.6328	20.2704
20-24	20.36	29.6856	29.683999999999997	20.2704
25-29	20.348	29.7968	29.6288	20.226399999999998
30-34	20.363999999999997	29.82	29.5848	20.231199999999998
35-39	20.612	29.836000000000002	29.3296	20.2224
40-44	20.2728	29.4288	29.8832	20.4152
45-49	20.355999999999998	29.566399999999998	29.688799999999997	20.3888
50-54	20.22	29.796	29.496	20.488
55-59	20.3712	29.6072	29.8688	20.152800000000003
60-64	20.2424	29.785600000000002	29.7024	20.269599999999997
65-69	20.3872	29.443200000000004	29.8176	20.352
70-74	20.408	29.6176	29.604000000000003	20.3704
75-79	20.388	29.676799999999997	29.4672	20.468
80-84	20.3112	29.727199999999996	29.6376	20.324
85-89	20.308799999999998	29.7184	29.439999999999998	20.5328
90-94	20.491200000000003	29.4144	29.7744	20.32
95-99	20.677599999999998	29.5648	29.3016	20.456
100-104	20.604	29.3408	29.6416	20.413600000000002
105-109	20.555200000000003	29.520000000000003	29.5504	20.3744
110-114	20.5232	29.4576	29.5232	20.496
115-119	20.4984	29.3368	29.580000000000002	20.5848
120-124	20.488799999999998	29.564	29.512	20.435200000000002
125-129	20.84	29.3368	29.3664	20.4568
130-134	20.846400000000003	29.1288	29.2744	20.7504
135-139	20.8568	29.280800000000003	29.0712	20.7912
140-144	20.7	29.360799999999998	29.1416	20.7976
145-149	20.837600000000002	29.0712	29.2384	20.8528
150	21.372	28.796	28.671999999999997	21.16
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.5
12	1.5
13	4.0
14	5.5
15	6.5
16	14.5
17	21.5
18	34.0
19	60.5
20	92.5
21	115.5
22	161.0
23	234.5
24	301.5
25	383.0
26	494.5
27	597.0
28	705.0
29	777.5
30	891.5
31	1048.0
32	1111.5
33	1188.0
34	1207.5
35	1176.5
36	1208.0
37	1178.0
38	1117.5
39	1024.0
40	930.5
41	848.5
42	723.5
43	628.0
44	559.5
45	470.5
46	374.5
47	320.0
48	262.0
49	201.5
50	172.0
51	150.0
52	109.5
53	85.5
54	75.0
55	53.0
56	39.0
57	43.5
58	49.5
59	44.5
60	41.5
61	43.5
62	58.0
63	81.5
64	102.5
65	132.0
66	155.0
67	174.5
68	201.0
69	219.0
70	240.0
71	247.5
72	241.5
73	241.0
74	246.5
75	244.5
76	218.5
77	191.5
78	165.0
79	139.0
80	108.0
81	70.0
82	51.5
83	38.0
84	20.0
85	14.0
86	7.0
87	2.5
88	2.5
89	1.0
90	0.5
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
150	25000.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	99.94800000000001
#Duplication Level	Percentage of total
1	99.896
2	0.104
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
>10	0.0
>50	0.0
>100	0.0
>500	0.0
>1k	0.0
>5k	0.0
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	0.0	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	0.0	0.0	0.0
16-17	0.0	0.0	0.0	0.0	0.0	0.0
18-19	0.0	0.0	0.0	0.0	0.0	0.0
20-21	0.0	0.0	0.0	0.0	0.0	0.0
22-23	0.0	0.0	0.0	0.0	0.0	0.0
24-25	0.0	0.0	0.0	0.0	0.0	0.0
26-27	0.0	0.0	0.0	0.0	0.0	0.0
28-29	0.0	0.0	0.0	0.0	0.0	0.0
30-31	0.0	0.0	0.0	0.0	0.0	0.0
32-33	0.0	0.0	0.0	0.0	0.0	0.0
34-35	0.0	0.0	0.0	0.0	0.0	0.0
36-37	0.0	0.0	0.0	0.0	0.0	0.0
38-39	0.0	0.0	0.0	0.0	0.0	0.0
40-41	0.0	0.0	0.0	0.0	0.0	0.0
42-43	0.0	0.0	0.0	0.0	0.0	0.0
44-45	0.0	0.0	0.0	0.0	0.0	0.0
46-47	0.0	0.0	0.0	0.0	0.0	0.0
48-49	0.0	0.0	0.0	0.0	0.0	0.0
50-51	0.0	0.0	0.0	0.0	0.0	0.0
52-53	0.0	0.0	0.0	0.0	0.0	0.0
54-55	0.0	0.0	0.0	0.0	0.0	0.0
56-57	0.0	0.0	0.0	0.0	0.0	0.0
58-59	0.0	0.0	0.0	0.0	0.0	0.0
60-61	0.0	0.0	0.0	0.0	0.0	0.0
62-63	0.0	0.0	0.0	0.0	0.0	0.0
64-65	0.0	0.0	0.0	0.0	0.0	0.0
66-67	0.0	0.0	0.0	0.0	0.0	0.0
68-69	0.0	0.0	0.0	0.0	0.0	0.0
70-71	0.0	0.0	0.0	0.0	0.0	0.0
72-73	0.0	0.0	0.0	0.0	0.0	0.0
74-75	0.0	0.0	0.0	0.0	0.0	0.0
76-77	0.0	0.0	0.0	0.0	0.0	0.0
78-79	0.0	0.0	0.0	0.0	0.0	0.0
80-81	0.0	0.0	0.0	0.0	0.0	0.0
82-83	0.0	0.0	0.0	0.0	0.0	0.0
84-85	0.0	0.0	0.0	0.0	0.0	0.0
86-87	0.0	0.0	0.0	0.0	0.0	0.0
88-89	0.0	0.0	0.0	0.0	0.0	0.0
90-91	0.0	0.0	0.0	0.0	0.0	0.0
92-93	0.0	0.0	0.0	0.0	0.0	0.0
94-95	0.0	0.0	0.0	0.0	0.0	0.0
96-97	0.0	0.0	0.0	0.0	0.0	0.0
98-99	0.0	0.0	0.0	0.0	0.0	0.0
100-101	0.0	0.0	0.0	0.0	0.0	0.0
102-103	0.0	0.0	0.0	0.0	0.004	0.0
104-105	0.0	0.0	0.0	0.0	0.004	0.0
106-107	0.0	0.0	0.0	0.0	0.004	0.0
108-109	0.0	0.0	0.0	0.0	0.004	0.0
110-111	0.0	0.0	0.0	0.0	0.004	0.0
112-113	0.0	0.0	0.0	0.0	0.004	0.0
114-115	0.0	0.0	0.0	0.0	0.004	0.0
116-117	0.0	0.0	0.0	0.0	0.004	0.0
118-119	0.0	0.0	0.0	0.0	0.004	0.0
120-121	0.0	0.0	0.0	0.0	0.004	0.0
122-123	0.0	0.0	0.0	0.0	0.004	0.0
124-125	0.0	0.0	0.0	0.0	0.004	0.0
126-127	0.0	0.0	0.0	0.0	0.004	0.0
128-129	0.0	0.0	0.0	0.0	0.004	0.0
130-131	0.0	0.0	0.0	0.0	0.004	0.0
132-133	0.0	0.0	0.0	0.0	0.004	0.0
134-135	0.0	0.0	0.0	0.0	0.004	0.0
136-137	0.0	0.0	0.0	0.0	0.004	0.0
138-139	0.0	0.0	0.0	0.0	0.004	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GAGCTCG	10	0.0071972376	144.0	1
AGGCAGA	10	0.0071972376	144.0	1
>>END_MODULE
