##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	S28_Skin_HiSeq_cami_sub25k.R2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	25000
Total Bases	3.7 Mbp
Sequences flagged as poor quality	0
Sequence length	150
%GC	41
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.12464	34.0	32.0	35.0	29.0	35.0
2	32.25744	35.0	32.0	35.0	29.0	35.0
3	32.30356	35.0	32.0	35.0	29.0	35.0
4	35.78476	38.0	36.0	38.0	33.0	38.0
5	35.77904	38.0	36.0	38.0	33.0	38.0
6	35.7604	38.0	36.0	38.0	33.0	38.0
7	35.79728	38.0	36.0	38.0	33.0	38.0
8	35.72564	38.0	36.0	38.0	33.0	38.0
9	37.40096	40.0	38.0	40.0	33.0	40.0
10-14	37.532303999999996	40.2	38.2	40.4	33.0	40.4
15-19	38.529	41.0	39.0	42.0	33.0	42.0
20-24	38.412888	41.0	39.0	42.0	32.6	42.0
25-29	38.037496	41.0	38.0	42.0	31.6	42.0
30-34	37.63032	41.0	37.4	42.0	31.0	42.0
35-39	37.231671999999996	40.8	36.6	42.0	30.6	42.0
40-44	36.644672	39.8	36.0	42.0	28.8	42.0
45-49	35.799256	38.8	35.0	41.2	27.2	42.0
50-54	34.83252	37.2	34.0	40.8	25.0	41.8
55-59	34.409776	36.2	34.0	40.4	24.4	42.0
60-64	33.55637599999999	36.0	33.0	39.2	23.0	41.6
65-69	32.685928000000004	36.0	32.0	37.6	21.0	40.6
70-74	32.726792	36.0	33.0	36.8	22.4	39.8
75-79	32.297072	36.0	33.0	36.0	21.4	38.0
80-84	31.766816	36.0	32.6	36.0	20.4	37.0
85-89	31.264776	36.0	32.0	36.0	17.0	36.2
90-94	30.877928000000004	35.0	31.6	36.0	12.4	36.0
95-99	30.66264	35.0	31.0	36.0	9.0	36.0
100-104	30.314376000000003	35.0	30.6	36.0	5.6	36.0
105-109	29.848599999999998	35.0	30.0	36.0	3.0	36.0
110-114	29.500848	35.0	30.0	36.0	3.0	36.0
115-119	29.0742	34.8	28.0	36.0	3.0	36.0
120-124	28.620568	34.0	27.6	36.0	3.0	36.0
125-129	27.987703999999997	34.0	25.8	36.0	3.0	36.0
130-134	27.355719999999998	34.0	24.8	36.0	3.0	36.0
135-139	26.628688	33.6	22.0	36.0	3.0	36.0
140-144	25.794351999999996	33.0	18.0	36.0	3.0	36.0
145-149	24.449863999999998	32.2	4.0	35.2	3.0	36.0
150	22.5978	30.0	3.0	35.0	3.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
28	6.0
29	88.0
30	1139.0
31	6296.0
32	11714.0
33	5377.0
34	378.0
35	2.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	20.028000000000002	30.236	29.192	20.544
2	20.552	29.5	29.792	20.156
3	20.064	29.564	29.992	20.380000000000003
4	20.024	29.404000000000003	30.236	20.336000000000002
5	20.596	28.876	29.7	20.828
6	20.24	29.94	29.731999999999996	20.088
7	20.132	29.736	29.459999999999997	20.671999999999997
8	20.624000000000002	29.799999999999997	29.492	20.084
9	20.564	29.536	29.868	20.032
10-14	20.436	29.855999999999998	29.466399999999997	20.241600000000002
15-19	20.3144	29.773600000000002	29.5072	20.4048
20-24	20.2024	29.819200000000002	29.529600000000002	20.4488
25-29	20.4032	29.5784	29.4744	20.544
30-34	20.4552	29.596	29.620800000000003	20.328
35-39	20.4728	29.498400000000004	29.4016	20.627200000000002
40-44	20.3568	29.3792	29.664	20.599999999999998
45-49	20.3808	29.435200000000002	29.662399999999998	20.5216
50-54	20.3928	29.5856	29.583199999999998	20.4384
55-59	20.3816	29.498400000000004	29.443200000000004	20.6768
60-64	20.5072	29.459200000000003	29.5592	20.4744
65-69	20.524	29.4904	29.4904	20.4952
70-74	20.752000000000002	29.4744	29.377599999999997	20.396
75-79	20.632	29.452	29.4536	20.4624
80-84	20.1648	29.612	29.483999999999998	20.7392
85-89	20.4264	29.496	29.6072	20.470399999999998
90-94	20.5952	29.252	29.376	20.7768
95-99	20.6216	29.5136	29.42	20.4448
100-104	20.5304	29.386400000000002	29.4456	20.6376
105-109	20.714399999999998	29.3672	29.365599999999997	20.552799999999998
110-114	20.6888	29.266399999999997	29.326400000000003	20.7184
115-119	20.8072	29.583199999999998	28.9704	20.6392
120-124	20.552	29.2584	29.2688	20.9208
125-129	20.8552	29.0616	29.2016	20.8816
130-134	21.0288	29.111199999999997	29.0352	20.8248
135-139	20.7744	28.8352	29.285600000000002	21.1048
140-144	21.1576	28.9	28.838399999999996	21.104
145-149	21.0424	28.6008	29.0448	21.312
150	21.416	28.948	28.608	21.028
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	2.0
15	8.0
16	11.5
17	12.5
18	32.0
19	48.5
20	65.0
21	108.0
22	151.5
23	203.0
24	260.5
25	343.5
26	440.5
27	511.0
28	634.0
29	782.0
30	906.0
31	997.0
32	1069.5
33	1127.5
34	1170.5
35	1212.0
36	1227.0
37	1217.5
38	1154.5
39	1081.0
40	973.0
41	872.5
42	789.0
43	686.0
44	594.0
45	511.5
46	416.0
47	334.5
48	289.5
49	236.5
50	193.0
51	150.5
52	110.0
53	83.0
54	67.0
55	53.5
56	50.5
57	49.0
58	39.0
59	42.0
60	60.0
61	64.0
62	66.0
63	87.0
64	114.0
65	143.5
66	154.0
67	175.5
68	229.0
69	237.0
70	238.0
71	256.5
72	253.5
73	244.0
74	250.0
75	236.0
76	201.0
77	174.5
78	143.0
79	117.0
80	87.5
81	58.5
82	41.5
83	28.0
84	12.0
85	5.5
86	4.0
87	2.5
88	1.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
150	25000.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	99.97200000000001
#Duplication Level	Percentage of total
1	99.944
2	0.055999999999999994
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
>10	0.0
>50	0.0
>100	0.0
>500	0.0
>1k	0.0
>5k	0.0
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	0.0	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	0.0	0.0	0.0
16-17	0.0	0.0	0.0	0.0	0.0	0.0
18-19	0.0	0.0	0.0	0.0	0.0	0.0
20-21	0.0	0.0	0.0	0.0	0.0	0.0
22-23	0.0	0.0	0.0	0.0	0.0	0.0
24-25	0.0	0.0	0.0	0.0	0.0	0.0
26-27	0.0	0.0	0.0	0.0	0.0	0.0
28-29	0.0	0.0	0.0	0.0	0.0	0.0
30-31	0.0	0.0	0.0	0.0	0.0	0.0
32-33	0.0	0.0	0.0	0.0	0.0	0.0
34-35	0.0	0.0	0.0	0.0	0.0	0.0
36-37	0.0	0.0	0.0	0.0	0.0	0.0
38-39	0.0	0.0	0.0	0.0	0.0	0.0
40-41	0.0	0.0	0.0	0.0	0.0	0.0
42-43	0.0	0.0	0.0	0.0	0.0	0.0
44-45	0.0	0.0	0.0	0.0	0.0	0.0
46-47	0.0	0.0	0.0	0.0	0.0	0.0
48-49	0.0	0.0	0.0	0.0	0.0	0.0
50-51	0.0	0.0	0.0	0.0	0.0	0.0
52-53	0.0	0.0	0.0	0.0	0.0	0.0
54-55	0.0	0.0	0.002	0.0	0.0	0.0
56-57	0.0	0.0	0.004	0.0	0.0	0.0
58-59	0.0	0.0	0.004	0.0	0.0	0.0
60-61	0.0	0.0	0.004	0.0	0.0	0.0
62-63	0.0	0.0	0.004	0.0	0.0	0.0
64-65	0.0	0.0	0.004	0.0	0.0	0.0
66-67	0.0	0.0	0.004	0.0	0.0	0.0
68-69	0.0	0.0	0.004	0.0	0.0	0.0
70-71	0.0	0.0	0.004	0.0	0.0	0.0
72-73	0.0	0.0	0.004	0.0	0.0	0.0
74-75	0.0	0.0	0.004	0.0	0.0	0.0
76-77	0.0	0.0	0.004	0.0	0.0	0.0
78-79	0.0	0.0	0.004	0.0	0.0	0.0
80-81	0.0	0.0	0.004	0.0	0.0	0.0
82-83	0.0	0.0	0.004	0.0	0.0	0.0
84-85	0.0	0.0	0.004	0.0	0.0	0.0
86-87	0.0	0.0	0.004	0.0	0.0	0.0
88-89	0.0	0.0	0.004	0.0	0.0	0.0
90-91	0.0	0.0	0.004	0.0	0.0	0.0
92-93	0.0	0.0	0.004	0.0	0.0	0.0
94-95	0.0	0.0	0.004	0.0	0.0	0.0
96-97	0.0	0.0	0.004	0.0	0.0	0.0
98-99	0.0	0.0	0.004	0.0	0.0	0.0
100-101	0.0	0.0	0.004	0.0	0.0	0.0
102-103	0.0	0.0	0.004	0.0	0.0	0.0
104-105	0.0	0.0	0.004	0.0	0.0	0.0
106-107	0.0	0.0	0.004	0.0	0.0	0.0
108-109	0.0	0.0	0.004	0.0	0.0	0.0
110-111	0.0	0.0	0.004	0.0	0.0	0.0
112-113	0.0	0.0	0.004	0.0	0.0	0.0
114-115	0.0	0.0	0.004	0.0	0.0	0.0
116-117	0.0	0.0	0.004	0.0	0.0	0.0
118-119	0.0	0.0	0.004	0.0	0.0	0.0
120-121	0.0	0.0	0.004	0.0	0.0	0.0
122-123	0.0	0.0	0.004	0.0	0.0	0.0
124-125	0.0	0.0	0.004	0.0	0.0	0.0
126-127	0.0	0.0	0.004	0.0	0.0	0.0
128-129	0.0	0.0	0.004	0.0	0.0	0.0
130-131	0.0	0.0	0.004	0.0	0.0	0.0
132-133	0.0	0.0	0.004	0.0	0.0	0.0
134-135	0.0	0.0	0.004	0.0	0.0	0.0
136-137	0.0	0.0	0.004	0.0	0.0	0.0
138-139	0.0	0.0	0.004	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CATGGGA	10	0.0071972376	144.0	6
GTGATAC	35	0.00362398	61.71429	9
>>END_MODULE
