##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	S6_Oral_HiSeq_cami_sub25k.R1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	25000
Total Bases	3.7 Mbp
Sequences flagged as poor quality	0
Sequence length	150
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.42544	35.0	32.0	35.0	32.0	35.0
2	33.62112	35.0	32.0	35.0	32.0	35.0
3	33.65912	35.0	32.0	35.0	32.0	35.0
4	37.09808	38.0	36.0	38.0	36.0	38.0
5	36.98324	38.0	36.0	38.0	36.0	38.0
6	36.96424	38.0	36.0	38.0	36.0	38.0
7	36.97412	38.0	36.0	38.0	36.0	38.0
8	36.92072	38.0	36.0	38.0	36.0	38.0
9	38.63716	40.0	38.0	40.0	36.0	40.0
10-14	38.862536	40.2	38.2	40.4	36.0	40.4
15-19	39.917728000000004	41.0	39.0	42.0	36.0	42.0
20-24	39.802768	41.0	39.0	42.0	35.4	42.0
25-29	39.466616	41.0	39.0	42.0	34.8	42.0
30-34	39.110776	41.0	38.8	42.0	34.0	42.0
35-39	38.754336	41.0	37.8	42.0	33.6	42.0
40-44	38.070152	40.2	36.2	42.0	32.2	42.0
45-49	37.333535999999995	39.4	36.0	41.4	31.2	42.0
50-54	36.625128000000004	38.2	35.0	41.0	30.4	42.0
55-59	36.300200000000004	37.0	35.0	40.8	30.6	42.0
60-64	35.739951999999995	36.0	35.0	40.0	30.0	42.0
65-69	34.998496	36.0	34.4	38.2	29.4	41.0
70-74	34.331424	36.0	34.0	37.0	28.6	40.0
75-79	33.665096	35.8	33.8	36.0	27.8	38.0
80-84	33.52628800000001	36.0	34.0	36.0	28.0	37.0
85-89	33.150023999999995	36.0	34.0	36.0	27.8	36.6
90-94	32.867976	36.0	33.8	36.0	26.2	36.0
95-99	32.562664	36.0	33.0	36.0	25.6	36.0
100-104	32.196799999999996	36.0	32.8	36.0	25.0	36.0
105-109	31.900232	35.2	32.0	36.0	23.2	36.0
110-114	31.478192	35.0	32.0	36.0	20.8	36.0
115-119	31.104375999999995	35.0	31.2	36.0	19.4	36.0
120-124	30.848055999999996	35.0	30.8	36.0	18.0	36.0
125-129	30.268647999999995	35.0	30.0	36.0	11.2	36.0
130-134	29.662879999999994	34.8	29.6	36.0	5.2	36.0
135-139	29.135168	34.0	28.4	36.0	3.0	36.0
140-144	28.416000000000004	34.0	27.2	36.0	3.0	36.0
145-149	27.20956	33.8	24.2	36.0	3.6	36.0
150	24.7232	31.0	16.0	35.0	3.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
30	1.0
31	19.0
32	701.0
33	7530.0
34	14256.0
35	2484.0
36	9.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	20.7	29.424	29.504	20.372
2	20.46	29.34	29.831999999999997	20.368
3	20.82	29.348000000000003	29.86	19.972
4	20.535999999999998	29.436	29.576	20.452
5	20.552	29.456	29.272	20.72
6	20.392	29.452	29.864	20.291999999999998
7	20.284	29.564	29.392000000000003	20.76
8	20.548	29.328	29.364	20.76
9	20.8	29.324	29.355999999999998	20.52
10-14	20.294400000000003	29.5456	29.852800000000002	20.3072
15-19	20.465600000000002	29.464000000000002	29.3928	20.677599999999998
20-24	20.3216	29.492800000000003	29.5528	20.6328
25-29	20.1664	29.438399999999998	29.7848	20.610400000000002
30-34	20.2968	29.438399999999998	29.7	20.564799999999998
35-39	20.408	29.7288	29.3136	20.5496
40-44	20.5112	29.4824	29.6184	20.388
45-49	20.5568	29.6536	29.628	20.1616
50-54	20.505599999999998	29.4752	29.6112	20.408
55-59	20.3768	29.3152	29.7024	20.6056
60-64	20.3632	29.4264	29.711199999999998	20.499200000000002
65-69	20.5896	29.4872	29.413600000000002	20.5096
70-74	20.5096	29.5304	29.509600000000002	20.4504
75-79	20.460800000000003	29.616799999999998	29.195999999999998	20.7264
80-84	20.7168	29.696	29.1528	20.4344
85-89	20.6696	29.5184	29.3256	20.4864
90-94	20.6496	29.4896	29.3624	20.4984
95-99	20.688000000000002	29.439999999999998	29.2184	20.6536
100-104	20.6128	29.326400000000003	29.4288	20.632
105-109	20.5736	29.260799999999996	29.4752	20.6904
110-114	20.6144	29.2648	29.403200000000002	20.7176
115-119	20.773600000000002	29.256	29.212	20.758399999999998
120-124	20.796799999999998	29.3584	29.392000000000003	20.4528
125-129	20.7328	29.5064	29.043999999999997	20.7168
130-134	20.8232	29.1096	29.132799999999996	20.9344
135-139	20.7344	29.2944	29.2752	20.696
140-144	21.113599999999998	29.128	28.9856	20.7728
145-149	20.9672	28.9912	28.9968	21.0448
150	21.556	28.092	28.688000000000002	21.664
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.5
9	0.5
10	0.5
11	1.0
12	1.5
13	1.5
14	2.5
15	6.0
16	10.0
17	13.5
18	18.0
19	30.0
20	45.0
21	64.5
22	87.0
23	103.0
24	139.0
25	184.5
26	219.5
27	277.0
28	338.0
29	379.0
30	438.5
31	540.5
32	629.0
33	721.5
34	814.5
35	916.5
36	1008.0
37	1084.0
38	1181.5
39	1245.5
40	1260.0
41	1273.5
42	1243.0
43	1191.0
44	1161.0
45	1073.5
46	950.0
47	825.5
48	742.0
49	663.5
50	550.0
51	474.0
52	422.5
53	386.0
54	346.0
55	285.0
56	259.5
57	235.0
58	220.0
59	182.5
60	145.0
61	130.0
62	110.5
63	86.5
64	65.0
65	53.0
66	31.5
67	21.0
68	17.0
69	17.0
70	11.5
71	10.0
72	15.5
73	9.0
74	3.5
75	5.5
76	6.5
77	5.0
78	5.0
79	4.0
80	2.0
81	1.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
150	25000.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	99.97200000000001
#Duplication Level	Percentage of total
1	99.944
2	0.055999999999999994
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
>10	0.0
>50	0.0
>100	0.0
>500	0.0
>1k	0.0
>5k	0.0
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	0.0	0.0	0.0	0.0	0.004	0.0
2	0.0	0.0	0.0	0.0	0.004	0.0
3	0.0	0.0	0.0	0.0	0.004	0.0
4	0.0	0.0	0.0	0.0	0.004	0.0
5	0.0	0.0	0.0	0.0	0.004	0.0
6	0.0	0.0	0.0	0.0	0.004	0.0
7	0.0	0.0	0.0	0.0	0.004	0.0
8	0.0	0.0	0.0	0.0	0.004	0.0
9	0.0	0.0	0.0	0.0	0.004	0.0
10-11	0.0	0.0	0.0	0.0	0.004	0.0
12-13	0.0	0.0	0.0	0.0	0.004	0.0
14-15	0.0	0.0	0.0	0.0	0.004	0.0
16-17	0.0	0.0	0.0	0.0	0.004	0.0
18-19	0.0	0.0	0.0	0.0	0.004	0.0
20-21	0.0	0.0	0.0	0.0	0.004	0.0
22-23	0.0	0.0	0.0	0.0	0.004	0.004
24-25	0.0	0.0	0.0	0.0	0.004	0.004
26-27	0.0	0.0	0.0	0.0	0.004	0.004
28-29	0.0	0.0	0.0	0.0	0.004	0.004
30-31	0.0	0.0	0.0	0.0	0.004	0.004
32-33	0.0	0.0	0.0	0.0	0.004	0.004
34-35	0.0	0.0	0.0	0.0	0.004	0.004
36-37	0.0	0.0	0.0	0.0	0.004	0.004
38-39	0.0	0.0	0.0	0.0	0.004	0.004
40-41	0.0	0.0	0.0	0.0	0.004	0.004
42-43	0.0	0.0	0.0	0.0	0.004	0.004
44-45	0.0	0.0	0.0	0.0	0.004	0.004
46-47	0.0	0.0	0.0	0.0	0.004	0.004
48-49	0.0	0.0	0.0	0.0	0.004	0.004
50-51	0.0	0.0	0.0	0.0	0.004	0.004
52-53	0.0	0.0	0.0	0.0	0.004	0.004
54-55	0.0	0.0	0.0	0.0	0.004	0.004
56-57	0.0	0.0	0.0	0.0	0.004	0.004
58-59	0.0	0.0	0.0	0.0	0.004	0.004
60-61	0.0	0.0	0.0	0.0	0.004	0.004
62-63	0.0	0.0	0.0	0.0	0.004	0.004
64-65	0.0	0.0	0.0	0.0	0.004	0.004
66-67	0.0	0.0	0.0	0.0	0.004	0.004
68-69	0.0	0.0	0.0	0.0	0.004	0.004
70-71	0.0	0.0	0.0	0.0	0.004	0.004
72-73	0.0	0.0	0.0	0.0	0.004	0.004
74-75	0.0	0.0	0.0	0.0	0.004	0.004
76-77	0.0	0.0	0.0	0.0	0.004	0.004
78-79	0.0	0.0	0.0	0.0	0.004	0.004
80-81	0.0	0.0	0.0	0.0	0.004	0.004
82-83	0.0	0.0	0.0	0.0	0.004	0.004
84-85	0.0	0.0	0.0	0.0	0.004	0.004
86-87	0.0	0.0	0.0	0.0	0.004	0.004
88-89	0.0	0.0	0.0	0.0	0.004	0.004
90-91	0.0	0.0	0.0	0.0	0.004	0.004
92-93	0.0	0.0	0.0	0.0	0.004	0.004
94-95	0.0	0.0	0.0	0.0	0.004	0.004
96-97	0.0	0.0	0.0	0.0	0.004	0.004
98-99	0.0	0.0	0.0	0.0	0.004	0.004
100-101	0.0	0.0	0.0	0.0	0.004	0.004
102-103	0.0	0.0	0.0	0.0	0.004	0.004
104-105	0.0	0.0	0.0	0.0	0.004	0.004
106-107	0.0	0.0	0.0	0.0	0.004	0.004
108-109	0.0	0.0	0.0	0.0	0.004	0.004
110-111	0.0	0.0	0.0	0.0	0.004	0.004
112-113	0.0	0.0	0.0	0.0	0.004	0.004
114-115	0.0	0.0	0.0	0.0	0.004	0.004
116-117	0.0	0.0	0.0	0.0	0.004	0.004
118-119	0.0	0.0	0.0	0.0	0.004	0.004
120-121	0.0	0.0	0.0	0.0	0.004	0.004
122-123	0.0	0.0	0.0	0.0	0.004	0.004
124-125	0.0	0.0	0.0	0.0	0.004	0.004
126-127	0.0	0.0	0.0	0.0	0.004	0.004
128-129	0.0	0.0	0.0	0.0	0.004	0.004
130-131	0.0	0.0	0.0	0.0	0.004	0.004
132-133	0.0	0.0	0.0	0.0	0.004	0.004
134-135	0.0	0.0	0.0	0.0	0.004	0.004
136-137	0.0	0.0	0.0	0.0	0.004	0.004
138-139	0.0	0.0	0.0	0.0	0.004	0.004
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTAGAGG	10	0.0071972376	144.0	2
AACTACA	10	0.0071972376	144.0	6
CAACAAC	40	0.00819905	18.0	80-84
>>END_MODULE
