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Unix for Bioinformatics Beginners

  • ssh (connect to remote computer such as a high performance computing cluster)
  • pwd (print working directory)
  • cd (change directory)
  • ls (list directory content)
  • mkdir (make a directory)
  • touch (creates an empty file)
  • nano (basic editor for editing text files)
  • cp (make a copy of files or folders)
  • mv (rename files/folder or move files or folders)
  • rm (to remove files or folders)
  • cat (print file content to screen)
  • less (page through file content)
  • column (display tabular data nicely aligned on screen)
  • wc (word count, line count and character count)
  • grep (pattern search)
  • sinteractive (request compute and time resources on Biowulf's compute node to perform data analysis and file transfers)
  • module (find, load, and unload applications installed on high performance computing clusters)
  • man (view manual for a command)

Tip

All Unix commands follow the syntax of command option(s) input. Think of the command as a verb in natural language. The input is what users would like the command to act on and option(s) changes the default way in which a command runs.

Connecting to Remote Computer

As bioinformatics often deal with dataset that are too large to be analyzed using compute resources on a personal computer, a motive for learning Unix command line is to enable scientists to perform bioinformatics analyses on high performance computing systems such as Biowulf at NIH. To connect to a remote computer, the ssh command construct below can be used and the breakdown is as follows.

  • ssh: The command for connecting to a remote computer.
  • username: The user name that is used to sign onto a remote computer such as high performance computing (for Biowulf, username will be the user's NIH user name). Username is followed by @ (ie. the "at" symbol).
  • remote: This is the name of the remote computer (Biowulf would be biowulf.nih.gov) to connect to.
ssh username@remote.computer

The command above could be annunciated as ssh username at remote.computer.

To sign onto Biowulf, the NIH high performance computing system, use the following. Again, replace username with the user's NIH user name.

ssh username@biowulf.nih.gov

Next, enter the user specific password for the remote computer. Note that nothing shows up when the password is being type, but keep typing and hit enter when ready to sign on.

The Command Prompt

In Unix, the command prompt is where users issue commands to interact with the computer. For example, upon connecting to Biowulf, users will see the prompt shown below. Replace username with the user's NIH user name. The prompt below tells users that they are connected to Biowulf and are currently in the home directory (denoted by ~).

[username@biowulf ~]$

Caution

The prompt will look different depending on the computer system. It can also be customized, so do not panic if a prompt does not look exactly like that above.

Definition

The working directory is the folder in a computer system in which a user is currently in.

It is a good idea to know which directory a user is currently in as data analysis progresses. To check, use the following.

pwd

For instance, upon signing on to Biowulf users will land in their home directory (also denoted by ~). The pwd command will give the directory path below (replace username with the user's specific user name).

/home/username

Note

In Unix, a directory path indicates where in the file system hierarchy a user is. Directory paths that start with / are known as absolute. Each part of the directory path is separated by / and followed by the name of the folder in the file system hierarchy.

Change Directory

Note

The user's home directory on Biowulf is not the place to store analysis input and output as it only has 16gb of storage space and users cannot request an increase in quota. The user's data directory can be used for this as storage quota can be increased at the request of the user.

To change directory use the cd command follow by the directory to change into. Upon signing onto Biowulf, users should change into their data directory to performing analyses. To do this use the following. Replace username with the user's specific user name.

cd /data/username

The pwd command can be used to check whether the directory change is successful.

pwd

If the directory change is successful, the following path will be shown, again replace username with the user's NIH user name.

/data/username

To go back to the user's home directory use cd, cd ~, or cd /home/username. Use cd .. to go back one directory. To go back two directories, use cd ../../. To go back three directories use cd ../../../ and so on.

Note

The examples below will be done using a folder called unix_demonstration in the instructor's /data folder. There is no need to follow along. To change into unix_demonstration from the /data folder, just do cd unix_demonstration.

Listing Directory Content

To view the files and subfolder within a directory, the ls command can be used. For instance, ls will retrieve the following items in the unix_demonstration directory when issued.

file.txt

Recall that a Unix command come with options that alter its default behavior. The -l option of ls gives a detailed view of each item in a directory. For instance, on the left hand column of the table below, lines that start with - are files while those starting with d are folders. The fifth column list the file or folder size. Note file.txt has a file size of 0 bytes since it is empty. Also, ls -l by default displays the files size in bytes.

-rw-r-----. 1 owner group    0 Jun 15 16:20 file.txt

Creating a New Directory

To make a new folder, use the mkdir command follow by the name of the folder.

mkdir folder

Recall

As mentioned previously, if the first column in the ls -l results starts with d then this indicates a directory, which is what folder should be.

ls -l
-rw-r-----. 1 owner group    0 Jun 16 10:09 file.txt
drwxr-x---. 2 owner group 4096 Jun 16 10:18 folder

Editing Files

Nano is a basic file editor that is built into Unix and enables editing of plain text files including txt, csv, fasta, genbank, gtf, and fastq. To edit a file using Nano, just use nano followed by the file name.

Unix is an operating system, just like Windows or MacOS.
Linux is a variety of Unix, and sometimes the names are used interchangeably.

For instance, to add the text above to file.txt, use:

nano file.txt

Then copy and paste the text into the editor. Hit control-x and then save to go back to the command prompt.

Note

If a file does not exist, then the nano command will create it as well as open the editor to enable editing. To create a blank file without opening the Nano editor, use the touch command instead.

touch bioinformatics.txt
ls -l
-rw-r-----. 1 owner group    0 Jun 16 18:13 bioinformatics.txt
-rw-r-----. 1 owner group  135 Jun 16 18:13 file.txt
drwxr-x---. 2 owner group 4096 Jun 16 10:18 folder

Copying Files and Folders

To copy a file use cp followed by the file name and the name of the duplicate.

For instance, to make a copy of bioinformatics.txt called btep_bioinformatics.txt, do:

cp bioinformatics.txt btep_bioinformatics.txt
ls
bioinformatics.txt  btep_bioinformatics.txt  file.txt  folder

The cp command can be used to copy a file into a folder. Just provide the file and the path to the folder in which to copy it. If a new name is desired for the duplicate, then append it to the destination folder path separated by a /. For instance, the command below will make a duplicate of bioinformatics.txt in folder called bioinformatics_for_beginners.txt.

cp bioinformatics.txt folder/bioinformatics_for_beginners.txt

Tip

To see contents of a folder other than those in the working directory, supply the path to the folder to the ls command.

ls folder
bioinformatics_for_beginners.txt

To copy a folder include the -r option in cp. The -r option recursively copies everything in a folder. The arguments are the folder to copy and the name of the duplicate folder.

cp -r folder bioinformatics_folder

The -1 option of ls prints directory content one item per line.

ls -1
bioinformatics_folder
bioinformatics.txt
btep_bioinformatics.txt
file.txt
folder
ls bioinformatics_folder
bioinformatics_for_beginners.txt

Renaming Files and Folders

The mv command can be used to rename files and folders.

To rename a file, use the mv command then provide the name of the file to rename and the new name of the file.

mv btep_bioinformatics.txt bioinformatics_for_noobies.txt
ls -1
bioinformatics_folder
bioinformatics_for_noobies.txt
bioinformatics.txt
file.txt
folder

To rename a folder, use the mv command and then provide the name of the folder to rename and the new name of the folder.

mv folder project_folder
ls -1
bioinformatics_folder
bioinformatics_for_noobies.txt
bioinformatics.txt
file.txt
project_folder

Moving Files and Folders

To move a file from one folder to another, the mv command can be used. The arguments in this application of the mv command is the file to be moved and the name of the folder in which the file will be moved to.

mv bioinformatics.txt bioinformatics_folder
ls bioinformatics_folder
bioinformatics_for_beginners.txt  bioinformatics.txt

Tip

mv can also move folders. Just supply the name of the folder to be moved as the first argument and the path of the destination folder.

Deleting Files and Folders

To delete a file use the rm command followed by the file name.

For instance, to delete bioinformatics_for_noobies.txt do:

rm bioinformatics_for_noobies.txt

Warning

There is no trash can or recycling bin in Unix. Once a file is deleted, it cannot be recovered. Use the -i option with rm to confirm deletion.

rm -i bioinformatics_for_noobies.txt

Type n for no and y for yes (to delete).

rm: remove regular empty file 'bioinformatics_for_noobies.txt'? n

Empty folders can be deleted using rmdir. On the other hand, folders with content can be removed using rm -r, where the -r option tells rm to delete the folder and recursively delete everything inside that folder.

rm -r -i project_folder

The command above will ask if the user wants to delete the project_folder and everything in it. Type y to confirm deletion. Users will be asked to confirm the deletion of each item in the folder.

rm: descend into directory 'project_folder'? y
rm: remove regular empty file 'project_folder/bioinformatics_for_beginners.txt'? y
rm: remove directory 'project_folder'? y
ls -1
bioinformatics_folder
bioinformatics_for_noobies.txt
file.txt

Viewing File Content

The cat command will print the entire content of a file to the terminal screen.

For instance, to view the content of file.txt in the terminal screen do:

cat file.txt
Unix is an operating system, just like Windows or MacOS.
Linux is a variety of Unix, and sometimes the names are used interchangeably.

The cat command can also be used to view fastq or fq files, which contain sequences from high throughput sequencers. For long files that take a while to completely print, hit control-c to exit the cat command and return to the prompt.

cat example_fastq.fq

Tabular data in the form of csv files can be displayed by cat as well.

cat example_rna_sequencing_counts.csv

Paging through Files

Rather than printing file content in its entirety to the terminal, users can page through files using less.

less example_rna_sequencing_counts.csv

Users can use the up and down arrow on the keyboard to scroll up/down the file to view content. The up/down arrows enable scrolling line by line.

Printing Tabular Data to Terminal Nicely Aligned

For tabular data in the form of csv files, which could contain multiple columns, the columns do not print to the terminal nicely aligned. The column command can fix this.

The options and arguments in the column command include:

  • Option -t: Creates a table.
  • Option -s: Prompts users to provide the column separator (ie. comma for csv files)
  • Argument: Name of the file which users would like to view (ie. example_rna_sequencing_counts.csv)
  • | is the pipe operator, which sends output from one command as input for another (ie.less to enable paging through the data table)
column -t -s ',' example_rna_sequencing_counts.csv | less

column command output
Geneid              HBR_1.bam  HBR_2.bam  HBR_3.bam  UHR_1.bam  UHR_2.bam  UHR_3.bam
U2                  0          0          0          0          0          0
CU459211.1          0          0          0          0          0          0
CU104787.1          0          0          0          0          0          0
BAGE5               0          0          0          0          0          0
ACTR3BP6            0          0          0          0          0          0
5_8S_rRNA           0          0          0          0          0          0
AC137488.1          0          0          0          0          0          0
AC137488.2          0          0          0          0          0          0
CU013544.1          0          0          0          0          0          0
CT867976.1          0          0          0          0          0          0
CT867977.1          0          0          0          0          0          0
CT978678.1          0          0          0          0          0          0
CU459202.1          0          0          0          0          0          0
AC116618.1          0          0          0          0          0          0
CU463998.1          0          0          0          0          0          0
CU463998.3          0          0          0          0          0          0
CU463998.2          0          0          0          0          0          0
U6                  0          0          0          0          0          0
LA16c-60D12.1       0          0          0          3          2          0
LA16c-13E4.3        0          0          0          0          0          1
LA16c-60D12.2       0          0          0          0          4          1
ZNF72P              0          0          0          0          1          0
BNIP3P2             0          0          0          0          2          0
YME1L1P1            0          0          0          0          0          0
OR11H1              0          0          0          0          0          0
LA16c-60G3.6        0          0          0          0          3          0
ARHGAP42P3          0          0          0          0          2          0
LA16c-23H5.4        0          0          0          0          1          0
LA16c-60G3.8        0          0          0          0          2          0
LA16c-60G3.7        0          0          0          0          0          0
LA16c-60G3.5        0          0          0          0          0          0
LA16c-2F2.8         0          0          0          0          3          0
NEK2P2              0          0          0          0          1          1
LA16c-2F2.5         0          0          0          0          0          0
NF1P6               0          0          0          1          4          0
MED15P7             0          0          0          0          2          0
POTEH               0          0          1          7          7          11
POTEH-AS1           0          0          0          0          2          0
RNU6-816P           0          0          0          0          0          0
LA16c-3G11.7        0          0          0          2          1          2
LA16c-83F12.6       0          0          0          3          1          0
GRAMD4P2            0          0          0          0          1          0
DUXAP8              10         4          9          250        199        213
LL22NC03-N64E9.1    0          1          0          16         9          19
BMS1P22             0          1          3          25         24         24
LL22NC03-N14H11.1   4          2          0          98         38         49
NBEAP3              0          0          0          5          3          0
LA16c-60H5.7        0          0          0          11         6          6
LA16c-4G1.4         0          0          0          0          0          0
LA16c-4G1.3         0          0          0          0          3          0
AC092854.1          0          0          0          0          0          0
ABCD1P4             0          0          0          0          2          0
LA16c-17H1.3        0          0          0          0          0          0
PABPC1P9            0          0          0          0          1          0
SLC9B1P4            0          0          0          0          1          0
ACTR3BP2            0          0          0          0          0          0
CHEK2P4             0          0          0          0          0          0
KB-67B5.17          0          0          0          0          0          0
KB-67B5.12          0          0          0          0          0          0
KCNMB3P1            0          0          0          2          1          1
CCT8L2              0          0          0          1          2          0
FABP5P11            0          0          0          0          0          0
AP000547.1          0          0          0          0          1          0
TPTEP1              19         23         20         141        94         122
KB-7G2.9            0          0          1          3          4          2
SLC25A15P5          0          0          0          2          2          4
PARP4P3             0          0          0          1          1          2
ANKRD62P1-PARP4P3   0          1          0          3          3          0
ANKRD62P1           0          0          0          2          0          1
KB-7G2.8            0          0          0          0          0          0
VWFP1               2          2          4          1          2          0
AC005301.8          0          0          0          1          0          0
XKR3                0          0          0          3          4          8
HSFY1P1             0          0          0          0          0          0
GPM6BP3             0          0          0          0          0          0
AC007064.22         0          0          0          0          0          0
ZNF402P             0          0          0          1          1          0
AC007064.24         1          3          2          3          0          1
MTND1P17            0          0          0          0          0          0
AC007064.25         0          0          0          0          0          0
AC006548.19         0          0          0          0          0          0
IGKV1OR22-5         0          0          0          0          0          0
IGKV2OR22-4         0          0          0          0          0          0
IGKV2OR22-3         0          0          0          0          0          0
IGKV3OR22-2         0          0          0          0          1          0
IGKV1OR22-1         0          0          0          0          0          0
AC006548.28         1          1          1          4          40         4
GAB4                0          0          0          0          0          0
AC006548.26         0          0          0          0          0          0
VN1R9P              0          0          0          0          0          0
CECR7               22         27         32         31         27         24
AC006946.16         7          6          4          3          3          4
AC006946.17         0          2          2          0          3          2
IL17RA              58         65         58         95         71         81
AC006946.12         0          1          1          1          1          1
CECR6               26         45         32         9          6          4
AC006946.15         3          3          1          0          2          0
CECR5               30         44         33         127        82         139
CECR5-AS1           0          2          2          1          2          1
CECR1               30         28         32         39         35         22
AC005300.5          0          0          0          1          0          0
FAM32B              0          1          0          0          0          0
CECR3               1          0          1          0          1          0
CECR9               0          0          0          0          0          0
RN7SL843P           0          0          0          0          0          0
CECR2               115        88         119        119        81         98
AC004019.10         1          5          1          0          1          1
CLCP1               1          2          0          0          0          0
DNAJA1P6            0          0          0          0          0          0
SLC25A18            100        111        74         6          8          7
AC004019.13         1          2          1          1          2          3
ATP6V1E1            392        454        391        244        181        203
BCL2L13             212        284        264        244        166        200
BID                 70         85         84         112        76         109
MIR3198-1           0          0          0          0          0          1
LINC00528           1          0          0          1          2          4
MICAL3              339        429        425        211        145        193
XXbac-B461K10.4     6          6          9          1          2          1
MIR648              0          0          6          0          0          0
XXbac-B476C20.14    2          7          2          2          1          1
XXbac-B476C20.13    2          9          4          1          2          3
RHEBP3              1          2          3          0          0          1
FLJ41941            0          0          0          0          2          0
XXbac-B476C20.9     3          5          4          2          3          5
PEX26               135        174        137        205        135        180
XXbac-B476C20.11    0          0          1          3          0          0
XXbac-B476C20.10    0          0          0          0          0          0
ARL2BPP10           0          0          0          0          0          0
TUBA8               13         13         15         2          2          1
AC008079.10         55         68         53         14         14         8
USP18               3          6          2          28         14         21
AC008079.9          0          0          0          0          0          0
GGTLC5P             0          0          0          0          0          0
AC011718.2          1          0          0          0          0          0
AC011718.3          0          0          0          0          0          0
PPP1R26P3           0          0          0          0          0          0
XXbac-B33L19.6      0          0          0          0          0          0
FAM230A             0          0          0          2          4          0
XXbac-B33L19.12     0          0          0          0          0          0
GGTLC3              0          0          0          0          0          0
Metazoa_SRP         0          0          1          0          2          2
XXbac-B33L19.15     0          0          0          0          0          0
TMEM191B            6          1          3          0          2          2
PI4KAP1             18         11         36         65         33         34
SCARNA17            0          0          0          0          0          0
RN7SKP131           0          0          0          0          0          0
RIMBP3              1          2          0          2          3          6
XXbac-B33L19.10     0          0          0          0          0          0
CA15P2              0          0          0          0          0          0
PPP1R26P2           0          0          0          0          0          0
PPP1R26P4           0          0          0          0          0          0
AC008132.15         0          0          0          0          1          0
GGT3P               0          0          0          0          0          1
AC008132.14         0          0          0          0          0          0
E2F6P1              0          0          0          3          0          0
AC008132.13         0          0          1          1          3          1
BCRP7               0          0          0          0          0          0
AC008103.3          0          0          0          0          0          0
AC008103.4          0          0          0          0          0          0
DGCR6               73         91         76         24         13         18
PRODH               102        127        123        44         34         39
AC007326.1          0          0          0          3          1          3
AC007326.10         0          1          0          0          3          0
DGCR5               196        239        201        8          9          8
AC007326.9          11         9          12         1          0          0
DGCR9               30         30         27         5          2          4
AC000095.9          0          0          0          0          0          0
AC000095.11         9          5          9          0          0          0
CA15P1              4          3          5          1          2          1
DGCR2               304        354        314        308        178        274
Y_RNA               4          2          2          5          2          3
DGCR11              4          10         13         19         6          14
AC004461.4          1          1          0          0          2          0
DGCR12              2          1          7          2          2          4
AC004471.9          5          4          5          4          3          5
AC004471.10         1          1          0          0          2          0
TSSK1A              0          0          0          0          2          1
DGCR14              46         50         37         77         39         64
TSSK2               0          0          0          0          0          0
GSC2                0          0          0          0          0          0
LINC01311           3          1          3          11         8          11
SLC25A1             32         50         41         226        138        216
CLTCL1              33         40         36         102        71         81
AC000081.2          0          0          1          0          1          0
SNORA15             0          0          0          0          1          0
KRT18P62            1          1          0          1          0          1
HIRA                20         15         10         59         27         39
C22orf39            64         74         76         100        88         90
RN7SL168P           0          1          0          4          2          1
MRPL40              29         36         31         113        70         91
AC000068.5          5          4          1          6          8          5
UFD1L               38         58         53         228        148        168
AC000068.9          0          0          0          0          0          0
AC000068.10         0          0          0          0          2          4
CDC45               2          1          0          224        154        175
CLDN5               60         86         57         2          2          0
LINC00895           0          0          0          0          0          0
AC000077.2          0          0          0          0          1          0
AC000067.1          0          0          0          0          0          0
SEPT5               481        635        547        145        97         110
GP1BB               0          0          0          0          0          0
TBX1                1          0          2          7          9          7
GNB1L               14         17         8          39         36         30
AC000089.3          1          0          0          2          1          1
C22orf29            83         123        95         211        149        168
TXNRD2              55         52         41         84         68         74
AC000078.5          2          9          2          8          4          4
COMT                96         113        87         205        111        166
MIR4761             0          0          1          1          0          0
ARVCF               143        157        135        86         35         52
TANGO2              36         47         38         68         48         43
MIR185              0          0          0          0          0          0
AC006547.13         7          17         15         13         9          16
AC006547.15         0          0          0          0          0          0
DGCR8               80         96         67         238        162        203
MIR3618             0          0          0          0          0          0
MIR1306             0          0          0          0          0          0
AC006547.8          0          0          0          0          0          0
TRMT2A              42         62         49         164        82         128
MIR6816             0          0          0          0          0          0
RANBP1              52         74         70         593        399        475
SNORA77             0          0          0          0          0          0
ZDHHC8              95         97         91         114        84         82
AC006547.14         6          5          4          3          3          2
XXbac-B444P24.8     0          1          3          0          0          0
LINC00896           0          3          0          7          4          7
RTN4R               74         93         84         19         11         11
MIR1286             1          3          0          0          0          0
XXbac-B444P24.10    1          1          0          1          4          0
DGCR6L              47         42         38         96         46         86
XXbac-B444P24.13    1          2          3          2          1          4
XXbac-B444P24.14    3          3          3          3          0          2
XXbac-B33L19.4      0          0          0          1          0          0
USP41               0          0          0          3          2          0
ZNF74               38         40         41         110        79         90
RNU6-225P           0          0          0          0          0          0
SCARF2              11         7          2          34         23         38
XXbac-B562F10.12    0          0          0          0          0          0
KLHL22              63         80         83         81         52         65
XXbac-B562F10.11    4          8          13         10         5          3
RNY1P9              0          0          0          0          0          0
RN7SL812P           0          0          3          1          0          3
KRT18P5             4          3          1          4          2          8
AC007731.1          0          0          0          0          0          0
MED15               94         110        87         258        156        210
AC007050.17         0          0          0          3          2          2
AC007050.18         0          0          1          0          0          0
SMPD4P1             2          4          2          1          3          1
IGLL4P              0          0          0          0          1          0
SLC9A3P2            0          0          0          0          0          2
AC007050.1          0          0          0          0          0          0
ABHD17AP4           0          1          3          0          2          3
POM121L4P           0          0          0          0          1          0
BCRP5               0          0          0          0          0          0
TMEM191A            5          7          5          0          4          6
PI4KA               1102       1211       1079       433        343        391
SERPIND1            13         11         8          158        109        124
SNAP29              33         48         44         114        82         76
AC007308.7          0          0          0          0          0          0
CRKL                343        423        367        758        482        613
XXbac-B135H6.15     5          3          4          1          6          0
AIFM3               85         82         100        7          13         10
LZTR1               140        179        126        297        188        223
XXbac-B135H6.18     4          5          1          1          2          1
THAP7               36         56         39         95         77         87
THAP7-AS1           4          7          6          9          2          4
TUBA3FP             2          1          2          6          6          5
P2RX6               35         33         31         23         19         27
SLC7A4              19         25         14         9          4          3
AC002472.11         0          0          1          0          0          0
MIR649              0          0          0          0          0          1
P2RX6P              5          2          2          0          1          2
LRRC74B             0          2          2          1          1          1
TUBA3GP             0          0          0          0          0          0
BCRP2               1          0          0          0          1          0
KB-1592A4.15        0          0          0          0          0          0
POM121L7            1          0          1          0          0          0
E2F6P2              0          1          0          0          0          0
FAM230B             0          0          0          0          1          0
KB-1592A4.14        0          0          0          0          0          0
GGT2                0          0          1          3          2          0
E2F6P3              0          0          0          0          0          0
POM121L8P           0          0          0          0          0          0
BCRP6               0          0          0          0          0          0
KB-1183D5.13        0          0          0          0          0          0
KB-1183D5.14        0          0          0          0          0          0
PPP1R26P5           0          0          0          0          1          0
KB-1183D5.18        0          0          0          0          0          0
KB-1183D5.16        0          0          0          0          0          0
RIMBP3B             0          1          0          0          2          2
RN7SKP63            0          0          0          0          0          0
HIC2                29         25         28         209        106        140
TMEM191C            1          1          6          1          5          3
PI4KAP2             28         26         20         96         60         67
RN7SKP221           0          0          0          0          0          0
RIMBP3C             0          0          0          0          1          0
UBE2L3              207        241        198        470        310        346
YDJC                18         28         24         155        83         125
CCDC116             2          0          1          5          3          2
KB-1440D3.14        0          0          0          2          1          1
SDF2L1              5          14         3          51         34         44
PPIL2               60         65         60         243        146        211
MIR301B             0          0          0          0          0          0
MIR130B             0          0          0          0          0          0
KB-1440D3.13        0          0          0          4          5          1
YPEL1               25         45         31         17         9          8
RN7SL280P           1          0          1          1          1          0
MAPK1               741        955        901        840        502        664
RNA5SP493           0          0          1          3          4          1
KB-1027C11.4        0          1          1          1          0          1
PPM1F               80         106        108        217        140        169
LL22NC03-86G7.1     26         50         28         69         74         70
TOP3B               2          0          1          12         10         7
PRAMENP             0          0          0          0          0          0
IGLVI-70            0          0          0          0          1          0
IGLV4-69            0          0          0          0          0          0
IGLVI-68            0          0          0          0          0          0
LL22NC03-23C6.13    0          0          0          0          1          0
LL22NC03-23C6.12    0          0          0          0          1          0
IGLV10-67           0          0          0          0          0          0
LL22NC03-23C6.15    0          0          0          0          0          0
IGLVIV-66-1         0          0          0          0          0          0
IGLVV-66            0          0          0          0          0          0
IGLVIV-65           0          0          0          0          0          0
IGLVIV-64           0          0          0          0          0          0
IGLVI-63            0          0          0          0          1          0
IGLV1-62            0          0          0          0          2          0
LL22NC03-88E1.18    0          0          0          0          0          0
IGLV8-61            0          0          0          0          0          0
LL22NC03-88E1.17    0          0          0          0          0          0
ABHD17AP5           0          0          0          0          0          0
LL22NC03-30E12.10   0          0          0          0          0          0
LL22NC03-30E12.11   0          0          0          0          0          0
IGLV4-60            0          0          0          0          0          0
LL22NC03-30E12.13   0          0          0          0          0          0
SOCS2P2             0          0          0          0          1          0
IGLVIV-59           0          0          0          0          0          0
IGLVV-58            0          0          0          0          0          0
BMP6P1              0          0          0          0          0          0
IGLV6-57            0          0          0          1          0          0
IGLVI-56            0          0          0          0          0          0
IGLV11-55           0          0          0          0          0          0
IGLV10-54           0          0          0          0          0          0
IGLVIV-53           0          0          0          0          1          0
TOP3BP1             0          0          0          1          0          2
LL22NC03-123E1.5    0          0          0          0          1          0
VPREB1              0          0          0          1          0          1
CH17-264L24.1       12         12         18         16         15         13
LL22NC03-2H8.5      2          0          1          2          6          0
LL22NC03-2H8.4      0          0          0          0          0          0
LL22NC03-80A10.6    9          11         3          10         3          13
BMS1P20             3          1          7          6          1          2
IGLV5-52            1          2          3          0          0          0
IGLV1-51            1          4          1          0          1          0
IGLV1-50            1          3          0          0          0          0
LL22NC03-80A10.11   0          0          0          0          1          0
IGLV9-49            0          0          0          0          1          0
LL22NC03-75A1.9     0          0          0          0          0          0
IGLV5-48            0          0          0          0          0          0
IGLV1-47            0          0          0          0          0          0
LL22NC03-22A12.9    0          0          0          0          1          0
IGLV7-46            0          0          0          0          0          0
LL22NC03-22A12.12   0          0          0          0          0          0
IGLV5-45            0          0          0          0          0          0
IGLV1-44            0          0          0          0          0          0
IGLV7-43            0          0          0          0          0          0
IGLVI-42            0          0          0          0          0          0
IGLVVII-41-1        0          0          0          0          0          0
IGLV1-41            0          0          0          0          0          0
IGLV1-40            0          0          0          0          1          0
ASH2LP1             0          0          0          0          0          0
IGLVI-38            0          0          0          0          1          0
IGLV5-37            0          0          0          0          0          0
IGLV1-36            0          0          0          0          1          0
KB-288A10.17        0          1          0          0          1          0
IGLV7-35            0          0          0          0          0          0
KB-288A10.19        0          0          1          0          1          0
ZNF280B             28         37         39         65         55         42
ZNF280A             0          0          0          11         11         14
PRAME               0          0          0          822        472        606
LL22NC03-63E9.3     0          0          0          1          1          0
IGLV2-34            0          0          0          0          0          0
IGLV2-33            0          0          0          0          0          0
IGLV3-32            0          0          0          0          0          0
IGLV3-31            0          0          0          0          1          0
IGLV3-30            0          0          0          0          0          0
BCRP4               0          0          0          0          0          0
POM121L1P           0          0          0          0          1          0
GGTLC2              0          0          0          0          1          0
IGLV3-29            0          0          0          0          1          0
AC244250.2          0          0          0          0          0          0
IGLV2-28            0          0          0          0          0          0
IGLV3-27            0          0          0          2          0          0
IGLV3-26            0          0          0          0          0          0
IGLVVI-25-1         0          0          0          0          0          0
IGLV3-25            0          0          0          0          2          0
LL22NC03-102D1.16   0          0          0          0          0          0
LL22NC03-102D1.18   0          0          0          0          0          0
IGLV3-24            0          0          0          0          0          0
IGLV2-23            0          0          0          0          0          0
AC244250.1          0          0          0          0          0          0
IGLVVI-22-1         0          0          0          0          0          0
IGLV3-22            0          0          0          0          0          0
IGLV3-21            0          0          0          0          0          0
IGLVI-20            0          0          0          0          0          0
IGLV3-19            0          0          0          0          1          0
LL22NC03-48A11.14   0          0          0          0          0          0
AC244250.4          0          0          0          0          0          0
IGLV2-18            0          0          0          0          0          0
IGLL5               0          0          0          1          0          0
IGLV3-17            0          0          0          0          0          0
IGLV3-16            0          0          0          0          0          0
IGLV3-15            0          0          0          0          0          0
AC244250.3          0          0          0          0          0          0
IGLV2-14            0          0          0          26         12         13
IGLV3-13            0          0          0          0          0          0
IGLV3-12            0          0          0          0          0          0
LL22NC03-24A12.8    0          0          0          0          0          0
LL22NC03-24A12.9    0          0          0          0          0          0
AC244157.1          0          0          0          0          0          0
IGLV2-11            0          0          0          0          0          0
LL22NC03-84E4.13    0          0          0          0          0          0
IGLV3-10            0          0          0          0          0          0
LL22NC03-84E4.11    0          0          0          0          0          0
IGLV3-9             0          0          0          0          1          0
IGLV2-8             0          0          0          0          1          0
MIR650              0          0          0          0          0          0
LL22NC03-84E4.8     0          0          0          0          1          0
IGLV3-7             0          0          0          0          0          0
IGLV3-6             0          0          0          0          0          0
AC245028.1          0          0          0          0          0          0
IGLV2-5             0          0          0          0          0          0
IGLV3-4             0          0          0          0          0          0
IGLV4-3             0          0          0          0          0          0
IGLV3-2             0          0          0          0          0          0
IGLV3-1             0          0          0          0          0          0
MIR5571             0          0          0          0          0          0
IGLJ1               0          0          0          0          0          0
IGLC1               0          0          0          0          0          0
IGLJ2               0          0          0          0          0          0
IGLC2               0          0          0          706        503        534
IGLJ3               0          0          0          0          1          0
IGLC3               0          0          0          1170       317        803
IGLJ4               0          0          0          0          0          0
IGLC4               0          0          0          0          0          0
IGLJ5               0          0          0          0          1          0
IGLC5               0          0          0          0          0          0
IGLJ6               0          0          0          0          0          0
IGLC6               0          0          0          2          1          2
IGLJ7               0          0          0          0          0          0
IGLC7               0          0          0          8          5          5
AP000361.2          0          0          0          0          0          0
AP000362.1          0          0          0          1          0          0
RSPH14              4          8          5          1          0          0
GNAZ                223        247        214        32         17         26
AC000029.1          0          1          0          0          0          0
U7                  0          0          0          0          0          0
RAB36               29         30         33         20         15         17
BCR                 228        357        302        332        204        270
BCRP8               0          1          0          0          0          0
RN7SL263P           1          1          0          1          0          2
FBXW4P1             2          4          5          8          6          10
AP000343.1          0          2          0          0          0          0
AP000343.2          0          0          1          0          0          0
CES5AP1             2          2          0          0          1          0
ZDHHC8P1            21         16         14         1          2          3
KB-1269D1.8         1          1          0          0          0          0
AP000344.3          0          0          0          0          1          1
AP000344.4          0          1          0          1          3          1
AP000345.1          0          0          1          0          1          0
AP000345.4          0          0          0          4          4          3
PCAT14              0          0          1          202        156        181
AP000345.2          0          0          0          0          2          0
IGLL1               0          0          0          0          0          2
KB-208E9.1          1          3          1          0          1          0
KB-1572G7.5         0          1          0          1          1          0
DRICH1              2          2          1          0          4          2
KB-1572G7.4         0          0          1          0          0          1
KB-1572G7.3         0          3          0          0          0          1
GUSBP11             1          0          1          1          0          1
AP000347.4          0          0          0          1          5          1
ASLP1               1          1          2          5          3          4
KB-1572G7.2         0          0          1          0          0          1
AP000347.2          4          4          8          12         8          4
RGL4                16         27         18         17         15         8
ZNF70               26         46         32         80         48         41
VPREB3              0          0          0          0          0          3
C22orf15            2          7          7          4          3          6
CHCHD10             44         53         39         46         36         48
MMP11               3          1          1          15         17         17
AP000349.2          0          0          0          0          0          0
SMARCB1             70         78         62         169        131        139
DERL3               4          8          7          81         43         75
KB-1125A3.11        0          3          0          1          0          2
SLC2A11             54         63         46         28         34         27
AP000350.10         0          0          0          0          0          0
KB-1125A3.12        4          4          1          8          5          6
RN7SL268P           1          1          1          4          1          2
MIF                 3          3          3          3          1          1
MIF-AS1             5          1          5          8          11         12
AP000350.5          11         14         12         30         24         27
AP000350.1          0          0          0          0          0          0
AP000350.6          2          2          1          3          1          7
AP000350.7          0          0          0          0          0          0
AP000350.8          0          0          0          0          0          0
GSTT2B              3          4          3          10         4          6
KB-1125A3.10        0          0          0          0          0          0
DDTL                3          3          3          2          17         5
KB-226F1.2          1          2          1          1          4          3
DDT                 4          5          4          18         8          13
AP000351.3          0          0          0          0          0          0
GSTT2               0          0          0          0          0          0
AP000351.4          0          0          0          1          0          0
GSTTP1              0          0          0          0          1          1
AP000351.13         0          0          0          0          1          0
CABIN1              280        320        309        509        296        374
KB-318B8.7          13         11         7          14         10         13
SUSD2               8          8          7          11         17         10
GGT5                22         31         23         10         4          3
AP000354.4          0          0          1          0          0          0
POM121L9P           1          1          2          0          1          1
BCRP1               0          0          0          0          0          0
AP000354.2          0          0          0          0          1          0
SPECC1L             64         80         70         156        82         101
SPECC1L-ADORA2A     0          1          0          0          1          2
ADORA2A             2          4          2          4          0          0
ADORA2A-AS1         2          5          5          6          12         4
UPB1                0          1          2          1          10         2
AP000355.2          0          0          0          0          1          0
GUCD1               62         62         62         384        214        290
SNRPD3              71         87         98         293        211        254
GGT1                9          14         13         86         42         61
LRRC75B             20         30         26         21         24         24
AP000356.2          0          0          0          0          0          0
BCRP3               2          1          2          5          5          8
POM121L10P          0          0          0          0          0          0
ARL5AP4             0          0          0          0          0          0
AP000357.4          0          0          0          0          1          0
LL22NC03-N95F10.1   0          0          0          0          0          0
AP000358.5          0          0          0          0          0          0
CRIP1P4             0          0          0          0          1          0
PIWIL3              0          0          0          1          3          0
SGSM1               151        187        161        5          8          7
SNORD56             0          0          1          0          0          0
TMEM211             0          0          1          0          1          0
KIAA1671            126        148        131        158        97         142
CTA-243E7.4         3          3          0          1          3          0
CTA-243E7.3         0          1          1          1          1          0
CTA-243E7.2         0          2          1          4          4          3
CTA-243E7.1         5          6          3          12         19         6
CTA-221G9.12        7          7          6          4          1          1
CTA-221G9.10        0          0          0          0          0          0
CRYBB3              0          1          1          1          0          0
CTA-221G9.7         0          1          0          0          0          0
CRYBB2              0          1          0          0          4          0
Z99916.1            0          0          0          0          0          0
CTA-221G9.11        0          0          0          0          0          0
RP3-462D8.2         0          0          0          0          1          0
IGLL3P              0          0          0          0          0          0
CTA-246H3.8         0          1          0          2          2          1
CTA-246H3.11        0          2          2          4          2          1
LRP5L               7          5          5          41         24         26
CTA-246H3.12        0          0          0          1          2          1
CTA-390C10.9        0          1          0          0          0          2
IGLVIVOR22-1        0          0          0          0          0          0
CRYBB2P1            24         27         23         54         37         54
MIR6817             0          0          0          0          0          0
CTA-390C10.10       10         18         11         12         8          4
AL008721.1          0          0          0          0          0          0
CTA-407F11.8        1          1          0          1          2          1
ADRBK2              206        283        260        170        134        129
CTA-407F11.7        0          0          1          2          3          5
YES1P1              3          5          4          9          4          4
RNA5SP494           0          0          0          0          0          0
MYO18B              0          0          0          86         85         66
CTA-407F11.9        0          0          0          2          1          3
CTA-125H2.2         0          0          0          1          6          4
Z98949.1            0          0          0          1          1          0
CTA-125H2.1         0          0          0          0          0          0
RN7SKP169           0          0          0          0          1          0
CTA-125H2.3         0          0          0          0          0          0
CTA-796E4.3         0          0          0          0          1          0
CTA-796E4.4         1          0          0          0          1          1
SEZ6L               399        508        463        16         17         22
RP11-259P1.1        3          5          2          0          0          0
RNA5SP495           0          0          0          0          0          0
CTB-1048E9.7        0          0          0          0          1          0
ASPHD2              44         56         80         18         13         24
HPS4                114        176        183        318        202        256
SRRD                35         37         43         62         47         51
TFIP11              60         93         52         141        77         96
CTA-445C9.14        14         23         13         27         13         17
TPST2               38         39         38         85         46         85
MIR548J             0          0          0          2          0          0
HMGB1P10            2          3          2          9          10         6
CRYBB1              2          0          1          3          3          4
CRYBA4              1          0          0          0          1          1
ISCA2P1             0          0          0          0          1          0
MIAT                429        453        443        43         34         35
MIAT_exon1          0          0          0          0          0          0
CTA-373H7.7         0          4          0          0          0          0
MIAT_exon5_1        0          0          0          0          0          0
MIAT_exon5_2        0          0          0          0          0          0
MIATNB              4          6          8          11         14         12
MIAT_exon5_3        0          0          0          0          0          0
LINC01422           2          4          6          3          9          2
RP1-40G4P.1         0          0          0          0          0          0
CTA-992D9.11        0          0          0          0          8          0
RNU6-1066P          0          0          0          0          0          0
CTA-992D9.10        0          0          0          0          0          0
CTA-992D9.9         0          0          0          0          0          0
CTA-992D9.6         1          0          0          0          2          0
CTA-992D9.8         0          0          0          1          4          0
CTA-992D9.7         0          0          0          0          1          0
CTA-503F6.2         0          0          0          0          1          0
RP5-1172A22.1       1          0          0          2          1          3
CTA-929C8.5         0          0          0          0          0          0
CTA-929C8.7         0          0          0          0          0          0
CTA-929C8.6         0          0          0          0          3          0
CTA-929C8.8         1          3          1          0          0          0
RP1-205F14P.1       0          0          0          1          0          0
RP11-46E17.6        0          0          0          0          0          0
RP1-231P7P.1        0          0          0          0          2          0
RP1-213J1P__B.1     0          0          0          2          1          1
RP1-213J1P__B.2     0          0          0          0          0          0
RP11-375H17.1       0          0          0          0          1          0
MN1                 44         34         31         123        93         124
PITPNB              80         92         64         111        87         87
TTC28-AS1           42         51         66         48         46         48
TTC28-AS1_1         0          0          0          0          0          0
MIR3199-2           1          0          0          1          0          1
TTC28-AS1_2         0          0          0          0          0          0
TTC28               87         123        79         191        108        106
TTC28-AS1_3         0          0          0          0          0          0
TTC28-AS1_4         0          0          0          0          0          0
RN7SL757P           1          0          0          0          0          0
SNORD42             0          0          0          0          0          0
MIR5739             0          0          1          3          2          2
RN7SL162P           0          0          0          0          0          1
CHEK2               1          5          3          98         82         99
HSCB                7          9          11         35         20         18
CCDC117             68         75         58         259        176        175
XBP1                67         102        79         1082       636        767
Z93930.1            0          0          0          1          0          1
CTA-292E10.6        4          7          2          10         12         16
CTA-292E10.8        1          0          0          4          3          2
CTA-292E10.7        0          0          0          0          0          0
ZNRF3               97         103        102        99         89         83
ZNRF3-IT1           1          6          3          4          3          2
ZNRF3-AS1           6          3          3          8          2          2
C22orf31            0          2          0          0          1          0
KREMEN1             50         62         36         75         59         69
CTA-747E2.10        1          0          2          0          0          0
Z95116.1            0          0          0          0          0          0
RNU6-810P           1          0          0          0          0          0
RNU6-1219P          0          0          0          0          0          0
EMID1               11         19         29         12         6          7
RHBDD3              23         40         33         79         57         67
CTA-984G1.5         2          1          3          9          3          5
EWSR1               346        356        357        920        675        835
GAS2L1              73         77         66         32         23         34
RASL10A             9          27         17         0          2          0
AC002059.10         0          0          1          1          1          0
AP1B1               230        272        256        358        244        272
MIR3653             0          0          0          0          0          0
SNORD125            0          0          0          0          0          0
AC000041.8          0          0          0          0          0          0
RFPL4AP6            0          0          0          0          1          0
RFPL1S              116        107        115        6          2          0
AC000041.10         0          0          0          0          0          0
RFPL1               0          0          0          0          0          0
AC000035.3          0          0          0          0          0          0
NEFH                194        265        253        27         24         21
THOC5               73         123        65         218        137        149
CTA-256D12.11       0          0          1          1          2          0
NIPSNAP1            105        131        119        291        188        233
NF2                 206        270        235        445        307        342
RPEP4               2          4          1          2          0          3
RP1-76B20.11        0          1          0          0          1          0
RP1-76B20.12        0          0          0          0          0          0
CABP7               18         21         25         2          1          6
ZMAT5               16         12         6          20         20         12
UQCR10              83         94         104        185        98         137
ASCC2               35         42         39         144        91         131
MTMR3               36         46         35         29         15         17
AC004819.1          1          0          1          0          1          0
RNU6-331P           0          0          0          0          0          0
AC003681.1          0          0          1          0          0          0
RP3-394A18.1        81         94         75         86         63         78
MIR6818             0          0          0          0          0          0
HORMAD2-AS1         2          0          0          1          1          0
CNN2P1              0          1          0          0          1          0
HORMAD2             0          0          0          1          5          0
CTA-85E5.7          0          0          0          0          0          0
CTA-85E5.6          0          0          0          0          0          0
RP3-438O4.4         0          0          0          0          2          0
RP1-102K2.6         0          0          0          2          0          0
LIF                 2          3          3          154        97         106
RP1-102K2.8         0          0          0          3          1          0
OSM                 0          0          1          0          0          2
RP1-102K2.9         2          3          0          0          1          1
GATSL3              5          6          3          2          0          1
RP1-130H16.18       0          0          0          0          0          0
TBC1D10A            13         15         14         39         30         39
SF3A1               184        232        179        388        259        285
CCDC157             3          15         9          9          8          7
RNF215              54         65         42         57         40         47
SEC14L2             53         81         68         42         33         34
RP4-539M6.19        0          0          0          0          0          0
AC004832.1          0          0          1          0          0          0
RP4-539M6.20        3          6          4          4          6          4
RNU6-564P           1          1          0          0          0          0
RP4-539M6.21        0          2          1          4          4          2
MTFP1               0          0          0          3          5          2
RP4-539M6.22        0          0          0          1          1          1
RP4-539M6.18        0          0          0          1          0          0
SEC14L3             1          0          0          0          2          0
RP4-539M6.14        0          0          0          0          0          0
SDC4P               0          0          0          0          0          0
SEC14L4             0          0          0          3          2          4
SEC14L6             4          12         3          3          7          5
SIRPAP1             0          0          0          0          0          0
GAL3ST1             19         18         21         4          6          4
PES1                65         80         70         271        202        255
RP1-56J10.8         0          0          0          0          1          0
TCN2                19         26         23         56         49         40
SLC35E4             18         32         26         21         12         13
DUSP18              8          13         9          12         10         9
CTA-963H5.5         1          2          0          3          0          1
OSBP2               119        137        98         113        57         84
AL022336.1          2          0          0          0          0          0
EIF4HP2             1          4          9          3          6          6
MORC2-AS1           1          2          3          5          2          3
MORC2               75         82         50         146        100        151
TUG1                188        227        207        545        349        397
TUG1_1              0          0          0          0          0          0
TUG1_2              0          0          0          0          0          0
TUG1_3              0          0          0          0          0          0
TUG1_4              0          0          0          0          0          0
RP3-430N8.11        0          0          0          0          0          0
RP3-430N8.10        0          0          0          0          0          0
RP3-412A9.17        0          0          0          2          5          2
RN7SL633P           0          0          0          0          0          0
SMTN                42         51         47         142        91         141
RP3-412A9.16        1          2          2          11         6          4
SELM                83         86         77         25         18         18
RP3-412A9.12        0          0          0          0          0          0
INPP5J              59         77         58         7          8          14
PLA2G3              1          5          2          13         12         8
RP3-412A9.15        0          0          0          0          0          0
MIR3928             0          0          0          0          0          0
RNF185              57         105        74         106        82         90
RNF185-AS1          0          0          0          0          0          0
LIMK2               96         119        103        113        70         95
RNU6-1128P          0          0          0          0          1          0
PIK3IP1             112        165        128        52         32         34
PIK3IP1-AS1         1          1          3          2          0          2
RNA5SP496           0          0          0          0          0          0
PATZ1               72         74         61         191        116        136
LINC01521           12         14         13         8          9          6
RNU6-338P           0          0          0          0          0          0
DRG1                83         105        96         191        143        151
EIF4ENIF1           95         107        96         94         77         78
RP11-247I13.11      0          0          2          1          1          0
RNU6-28P            1          0          0          0          1          1
SFI1                112        125        107        142        102        134
H2AFZP6             1          0          1          0          0          0
RP11-247I13.6       0          0          0          0          0          0
RP11-247I13.3       0          1          1          4          1          4
PISD                195        250        233        156        95         108
MIR7109             0          0          0          0          0          0
PRR14L              219        269        234        418        294        333
DEPDC5              51         79         76         77         50         65
RN7SL20P            0          0          0          1          2          1
CTA-440B3.1         0          0          1          1          2          0
RNU6-201P           0          0          0          0          0          0
RP1-180M12.1        0          1          2          0          0          0
C22orf24            0          6          6          4          2          3
YWHAH               1029       1363       1146       336        220        293
CTA-342B11.1        0          0          0          1          0          0
CTA-342B11.2        0          0          0          0          2          0
RN7SL305P           0          0          0          0          0          0
SC22CB-1E7.1        0          0          0          0          0          0
SLC5A1              0          0          0          0          6          0
AP1B1P1             0          0          0          0          1          0
RP1-127L4.10        0          0          0          0          0          0
AP1B1P2             0          0          0          0          0          0
RP1-127L4.7         0          0          0          1          0          0
C22orf42            0          2          0          0          1          1
RP1-90G24.8         0          0          0          0          0          0
RFPL3-AS1_1         0          0          0          0          0          0
RFPL2               5          3          10         1          1          0
AL008723.1          0          0          0          1          0          0
IGLCOR22-1          0          0          0          0          0          1
RP1-90G24.10        4          7          3          2          3          1
SLC5A4              7          12         5          13         9          4
CPSF1P1             41         43         42         6          12         10
RP1-90G24.6         1          1          3          0          0          0
RP1-90G24.11        0          3          0          1          0          0
RP1-149A16.12       0          1          1          1          0          0
RFPL3               0          0          0          0          0          0
IGLCOR22-2          0          0          0          0          0          0
RFPL3S              1          1          1          0          0          1
RFPL3-AS1_2         0          0          0          0          0          0
RP1-149A16.3        1          3          2          1          1          1
IGLVIVOR22-2        0          0          0          0          0          0
RP1-149A16.17       0          1          0          4          2          0
RP1-149A16.16       2          0          0          2          0          1
RTCB                109        150        124        339        182        249
BPIFC               0          0          0          0          2          0
FBXO7               163        181        194        426        375        371
SYN3                74         110        78         11         12         9
LL22NC03-104C7.1    1          4          5          1          1          1
RNA5SP497           0          0          0          0          0          0
LL22NC01-116C6.1    0          0          1          0          1          0
TIMP3               214        271        243        805        491        604
CTA-415G2.2         0          0          0          0          0          0
RP1-302D9.1         0          0          0          0          0          0
RP1-302D9.2         0          0          0          0          0          1
RP1-302D9.5         0          0          0          0          1          0
LARGE               173        213        199        83         58         84
RP1-302D9.3         0          0          0          0          0          0
SC22CB-1D7.1        1          1          2          3          1          0
MIR4764             1          0          0          0          0          0
SNORA76             0          0          0          0          0          0
SNORA50             0          0          0          0          0          0
LARGE-IT1           0          0          0          0          0          1
LARGE-AS1           7          6          5          5          7          5
LL22NC03-32F9.1     0          0          0          1          0          0
LL22NC03-86D4.1     0          0          0          0          1          0
LL22NC03-13G6.2     0          1          2          0          0          0
RP1-101G11.2        0          0          0          0          0          0
RP1-101G11.3        0          0          0          3          4          2
RP1-288L1.5         2          3          0          0          1          0
RP1-288L1.4         0          0          1          0          0          0
ISX-AS1             1          0          0          2          1          1
ISX                 0          0          0          6          4          1
LINC01399           0          0          0          1          0          2
CTA-714B7.7         0          0          0          0          0          0
RP3-323A16.1        0          0          1          75         77         62
CTA-714B7.4         0          0          0          0          0          0
COX7BP1             0          0          0          0          0          0
RNU7-167P           0          0          0          0          0          0
HMGXB4              32         45         49         124        103        102
RP3-510H16.3        2          0          1          0          0          0
TOM1                99         119        103        84         56         68
MIR3909             1          0          0          1          1          0
MIR6069             0          0          0          0          0          0
CTA-286B10.7        0          0          0          1          0          0
CTA-286B10.8        0          0          0          0          0          0
HMOX1               13         19         13         83         51         81
MCM5                29         32         34         544        294        446
RP4-569D19.5        0          0          0          4          1          5
RP4-569D19.8        0          0          0          0          0          0
RASD2               55         81         60         15         15         17
MB                  0          1          0          9          8          8
CITF22-62D4.1       0          0          0          0          1          0
APOL6               64         54         56         331        197        254
RP1-41P2.7          0          0          0          0          0          0
MRPS16P3            0          0          0          0          0          0
APOL5               0          0          1          0          0          0
RBFOX2              621        738        711        515        367        405
NDUFA9P1            3          3          2          4          4          3
RP1-78B3.1          10         8          4          21         23         18
CTA-212A2.4         0          0          0          0          0          0
CTA-212A2.3         0          0          0          0          9          0
CTA-212A2.2         0          0          0          0          0          0
CTA-212A2.1         1          0          1          0          1          0
APOL3               8          6          12         39         33         19
Z95114.4            0          0          0          0          1          0
Z95114.3            0          0          0          0          0          0
Z95114.5            0          0          0          0          1          0
Z95114.6            0          0          0          0          1          0
Z95114.7            0          0          0          0          0          0
MTND1P10            0          0          0          0          1          0
APOL4               10         6          8          9          5          4
APOL2               113        151        135        148        112        112
APOL1               4          9          6          87         65         76
MYH9                449        512        485        2685       1608       2111
MIR6819             0          0          0          1          1          0
RP4-633O19__A.1     1          2          1          2          0          0
RPS15AP38           0          0          0          0          1          0
BX470187.1          0          0          0          0          0          0
RP5-1119A7.14       0          1          3          0          0          0
TXN2                27         41         39         82         49         39
FOXRED2             74         108        107        218        104        122
EIF3D               103        119        131        745        494        640
RP5-1119A7.11       0          0          0          0          0          1
RP5-1119A7.10       0          0          0          0          0          0
RP5-1119A7.17       41         56         44         0          0          0
CACNG2              33         34         48         0          1          0
RP1-293L6.1         3          2          4          0          0          0
IFT27               53         58         56         77         57         60
PVALB               100        129        102        25         17         15
CITF22-24E5.1       2          0          0          0          0          0
CTA-833B7.2         0          0          1          1          4          1
NCF4                3          7          1          24         17         15
CSF2RB              2          4          4          50         42         40
CSF2RBP1            0          0          0          0          1          0
LL22NC01-81G9.3     0          0          0          0          1          0
TEX33               0          0          0          0          0          0
TST                 18         21         18         88         61         51
MPST                34         29         33         131        76         112
KCTD17              47         74         73         32         21         14
RN7SKP214           0          0          0          1          0          0
TMPRSS6             1          3          0          5          5          3
RP5-1170K4.7        0          0          0          0          1          0
IL2RB               1          0          0          6          6          4
AL022314.1          0          0          0          0          1          0
RP1-151B14.6        0          0          0          1          0          0
C1QTNF6             11         8          10         85         65         67
SSTR3               30         36         48         14         12         12
RP1-151B14.9        0          0          0          0          0          0
RAC2                8          8          5          111        79         92
CYTH4               13         18         16         22         19         12
ELFN2               106        137        114        19         17         23
RP1-63G5.7          1          2          1          1          3          0
RP1-63G5.8          0          0          0          0          0          0
MFNG                4          5          6          75         30         49
CARD10              2          5          5          97         76         74
RP5-1177I5.3        1          1          0          0          0          0
CDC42EP1            68         100        65         144        81         126
LGALS2              0          0          0          7          5          3
GGA1                119        155        128        307        193        221
SH3BP1              20         13         16         55         34         41
Z83844.1            1          3          1          7          2          3
PDXP                5          6          5          2          1          1
RN7SL385P           0          1          0          0          0          0
LGALS1              75         93         78         729        447        618
NOL12               9          10         15         28         17         19
RP1-37E16.12        0          0          0          0          0          0
TRIOBP              36         28         25         164        103        142
H1F0                106        146        112        252        167        190
GCAT                34         27         33         76         56         52
GALR3               1          0          2          1          1          0
ANKRD54             33         48         55         91         50         73
MIR658              0          0          0          0          0          0
MIR659              0          0          0          1          0          0
EIF3L               191        222        206        704        454        597
RP5-1014D13.2       12         23         15         48         32         20
RNU6-900P           0          0          0          0          0          0
MICALL1             52         64         62         149        96         114
RP5-1039K5.12       1          8          1          4          2          1
C22orf23            2          1          5          9          6          5
AL031587.1          0          2          0          0          0          1
RP5-1039K5.19       167        192        150        105        83         72
RP5-1039K5.17       0          0          0          0          0          0
POLR2F              15         28         17         12         8          7
MIR6820             0          0          0          0          0          0
SOX10               0          0          0          0          0          0
MIR4534             0          0          0          0          0          0
RP5-1039K5.16       6          3          10         4          7          0
RP5-1039K5.18       0          0          1          0          1          0
PICK1               79         81         85         46         33         60
RP5-1039K5.13       0          0          0          0          0          0
SLC16A8             9          13         11         11         5          6
BAIAP2L2            5          17         8          18         8          9
CTA-228A9.3         10         3          3          4          2          3
PLA2G6              61         57         62         86         48         50
CTA-228A9.4         2          3          4          13         7          6
MAFF                31         27         20         97         63         68
TMEM184B            207        235        215        191        140        170
RP1-5O6.5           0          0          0          1          0          0
RN7SL704P           0          0          0          0          1          0
AL020993.1          0          0          0          0          0          0
CSNK1E              232        229        222        592        338        447
RP3-449O17.1        10         15         10         30         28         23
RP1-5O6.4           2          0          1          6          4          3
RP3-434P1.6         0          0          0          0          0          0
KCNJ4               49         70         50         2          1          3
KDELR3              6          7          7          106        92         91
DDX17               1532       1797       1637       2814       1818       2243
DMC1                2          4          0          15         11         8
RP1-199H16.6        1          0          0          0          0          0
FAM227A             16         12         17         40         48         22
CBY1                17         19         13         25         22         38
RP3-508I15.10       3          1          0          0          4          2
RP3-508I15.9        12         7          15         7          9          8
TOMM22              52         67         51         178        112        156
JOSD1               149        161        133        261        204        210
GTPBP1              146        187        152        335        182        247
PRDX3P1             3          5          2          11         3          10
RP3-508I15.18       0          0          0          0          2          0
SUN2                289        369        314        266        151        233
RP3-508I15.19       0          2          0          4          1          2
RP3-508I15.20       1          1          1          2          3          3
RP3-508I15.14       1          0          2          3          0          1
RP3-508I15.21       1          5          2          4          9          6
RP3-508I15.22       0          1          1          4          0          2
DNAL4               19         42         34         46         18         27
NPTXR               390        446        394        69         59         50
CBX6                738        873        830        362        240        300
CTA-150C2.13        0          0          0          0          0          0
APOBEC3A            0          0          0          0          1          0
APOBEC3B            1          0          0          19         22         15
APOBEC3B-AS1        0          0          0          0          0          0
APOBEC3C            4          3          3          80         48         59
APOBEC3D            1          1          2          16         8          9
APOBEC3F            1          1          0          11         6          7
APOBEC3G            4          9          3          41         25         22
CTA-150C2.20        0          0          0          0          0          0
RP4-742C19.12       0          2          1          0          0          0
APOBEC3H            4          3          0          0          0          0
CBX7                185        257        208        25         21         24
COX5BP7             0          0          0          0          0          0
RP4-742C19.13       6          19         10         8          6          6
FUNDC2P4            0          0          0          0          0          0
PDGFB               34         45         32         40         28         43
RP3-333H23.8        0          0          0          0          0          1
RPL3                659        761        721        4027       2406       3047
SNORD83B            0          0          0          0          0          0
SNORD83A            2          0          0          7          5          2
AL022326.1          0          0          0          1          1          3
SNORD43             0          0          0          0          0          0
SYNGR1              763        997        870        54         51         42
RP3-333H23.9        1          2          2          0          0          0
TAB1                99         91         70         82         75         86
MGAT3               257        328        274        35         15         24
MGAT3-AS1           5          9          8          1          2          1
MIEF1               95         122        114        312        164        204
ATF4                291        358        307        1354       819        1065
RPS19BP1            72         88         72         129        95         95
CACNA1I             106        139        136        2          7          0
ENTHD1              0          0          0          2          3          3
RP1-172B20.6        0          0          0          0          1          0
RN7SKP210           0          0          0          0          0          0
UQCRFS1P1           1          5          6          4          8          4
GRAP2               1          0          1          64         45         44
RP3-370M22.8        0          0          0          8          1          7
FAM83F              0          1          6          28         20         14
RP3-496C20.1        0          2          0          1          0          2
TNRC6B              582        686        592        528        327        405
RPL7P52             2          4          2          3          2          2
ADSL                40         41         48         302        176        212
RP5-1042K10.14      0          0          1          1          0          0
SGSM3               109        136        114        147        104        131
RP5-1042K10.10      1          2          1          4          4          5
MKL1                139        132        104        137        81         93
RP5-1042K10.13      0          0          1          1          1          3
RP5-1042K10.12      2          0          2          0          4          0
RP4-591N18.2        4          10         8          11         7          9
AL031594.1          1          1          1          2          2          0
COX6B1P3            1          0          0          1          0          0
RPL4P6              1          2          1          8          5          11
GAPDHP37            0          1          0          0          1          0
MCHR1               12         21         17         2          3          2
SLC25A17            39         39         40         119        96         116
RP3-408N23.4        1          1          3          5          1          1
JTBP1               1          1          0          6          1          4
MIR4766             0          0          0          2          0          1
ST13                389        470        463        880        640        760
XPNPEP3             31         43         39         149        105        122
DNAJB7              2          2          0          2          1          0
RNU6-379P           0          0          0          0          0          1
RBX1                50         68         60         122        77         91
AL080243.1          0          0          0          0          0          0
RP11-12M9.3         0          0          0          0          0          0
RP11-12M9.4         0          0          0          0          0          0
EP300               302        403        405        569        328        404
MIR1281             0          0          0          0          0          0
RNU6-375P           0          0          0          0          1          0
RP1-85F18.6         0          3          0          3          3          1
EP300-AS1           1          2          0          1          1          2
LRRC37A14P          0          1          0          0          0          1
L3MBTL2             91         96         83         108        82         88
RP4-756G23.5        6          8          9          2          5          6
CHADL               32         40         39         2          3          1
RANGAP1             256        316        267        464        296        392
MIR6889             0          0          0          0          0          0
ZC3H7B              417        531        475        453        288        372
TEF                 335        384        341        43         34         28
RNU6-495P           0          2          0          0          0          0
CTA-223H9.9         0          0          2          4          1          0
TOB2                203        279        197        173        116        145
PHF5A               34         36         17         134        79         89
ACO2                301        366        347        254        168        199
POLR3H              106        93         91         131        77         96
CSDC2               72         94         65         6          5          1
PMM1                34         40         55         51         31         25
DESI1               129        188        159        190        134        135
XRCC6               365        415        378        1431       973        1121
NHP2L1              168        201        178        366        219        291
RNU6-476P           0          1          0          3          1          0
C22orf46            34         36         32         56         46         49
MEI1                1          5          1          8          6          2
HMGN2P10            0          1          0          0          0          0
RNU6ATAC22P         0          0          0          0          0          0
CCDC134             9          24         19         39         24         30
RP5-821D11.7        12         14         13         34         18         23
SREBF2              497        669        575        627        384        539
MIR33A              0          1          2          0          0          0
SHISA8              33         31         18         0          0          4
TNFRSF13C           4          2          2          11         9          12
MIR378I             0          0          0          0          0          0
CENPM               0          3          1          65         35         52
LINC00634           28         16         21         0          1          1
SEPT3               721        907        863        54         38         55
CTA-250D10.19       4          3          4          0          1          0
WBP2NL              14         11         8          5          9          2
SLC25A5P1           0          0          1          0          1          0
NAGA                30         43         53         144        107        132
FAM109B             9          3          4          6          5          6
SNORD13P1           0          0          0          0          1          0
SMDT1               25         42         34         28         15         25
NDUFA6              67         101        81         167        109        126
RP1-257I20.14       2          1          2          8          4          2
NDUFA6-AS1          10         18         21         15         6          11
OLA1P1              1          0          3          3          0          0
CYP2D6              15         23         16         17         9          4
RP4-669P10.19       2          1          0          4          1          5
RP4-669P10.16       1          3          0          2          0          0
RP4-669P10.20       4          6          5          2          2          1
CYP2D7              6          9          8          2          3          10
CYP2D8P             2          3          4          6          7          7
TCF20               205        259        209        455        281        355
OGFRP1              0          3          1          13         6          7
Z83851.4            1          2          1          8          5          5
CTA-989H11.1        1          3          1          8          1          4
LINC01315           1          3          5          2          4          1
NFAM1               5          5          11         8          9          9
CTA-126B4.7         0          0          0          1          1          2
SERHL               4          3          5          2          2          0
RRP7A               59         89         80         157        86         117
Z93241.1            0          0          0          0          0          0
SERHL2              6          11         7          18         14         19
RRP7BP              20         30         18         52         42         45
RN7SKP80            284        319        228        250        413        377
RNU6-513P           0          0          0          0          0          0
POLDIP3             139        155        150        263        169        210
RNU12               6          10         7          17         4          11
CYB5R3              126        169        136        372        250        309
ATP5L2              0          3          1          2          0          0
A4GALT              8          5          2          36         23         25
RPL5P34             0          0          0          0          0          1
GOLGA2P4            0          1          0          0          1          0
RP1-47A17.1         0          0          1          1          2          1
ARFGAP3             53         65         44         187        145        130
PACSIN2             114        127        111        261        174        221
AL049758.2          0          1          0          1          0          0
AL022476.2          5          3          1          0          4          0
TTLL1               16         29         26         10         12         19
AL022237.3          0          1          0          0          0          0
BIK                 1          3          6          4          5          6
MCAT                19         27         25         43         27         35
TSPO                12         10         13         136        88         110
TTLL12              57         94         59         278        212        234
SCUBE1              1          5          3          11         15         7
Z82214.3            0          0          0          0          0          0
Z82214.2            0          0          0          0          0          0
Z99756.1            0          0          0          1          0          0
RP4-754E20__A.5     1          0          0          1          3          0
MPPED1              122        154        138        1          3          3
EFCAB6-AS1          2          3          0          0          2          0
EFCAB6              36         43         35         6          4          3
RP3-388M5.8         1          0          1          0          0          1
HMGN2P9             0          0          0          0          0          0
RP3-388M5.9         3          3          6          2          4          5
SULT4A1             290        334        288        5          7          3
PNPLA5              3          2          2          0          3          0
Z97055.1            0          0          0          0          0          0
PNPLA3              6          5          5          45         37         38
SAMM50              45         46         50         132        118        100
RP4-796I17.5        0          1          0          0          0          2
PARVB               69         89         68         162        134        145
CTA-414D7.1         3          6          4          10         10         10
RP4-671O14.6        3          7          5          18         15         8
PARVG               7          10         8          18         19         9
RP4-671O14.5        0          0          1          3          1          0
RP4-671O14.7        0          3          0          3          7          3
KIAA1644            149        205        176        162        111        134
RP1-32I10.10        0          0          0          0          0          0
RP1-32I10.11        0          0          0          0          0          0
CTA-397C4.2         0          0          0          0          0          0
MRPS18CP6           0          0          0          0          0          0
RP5-1033E15.3       0          0          0          0          0          0
LDOC1L              174        261        176        237        149        180
KRT18P23            0          0          0          0          2          0
LINC00207           0          0          0          0          4          0
LINC00229           0          0          0          0          0          0
ANP32BP2            0          0          0          0          0          0
PRR5                10         5          6          26         23         15
PRR5-ARHGAP8        0          1          0          1          1          0
ARHGAP8             2          1          2          6          6          3
PHF21B              15         14         9          16         16         12
RP1-127B20.4        1          1          0          1          1          0
RP4-753M9.1         1          5          0          5          4          2
NUP50-AS1           27         24         31         32         29         28
CTA-217C2.2         1          3          0          6          2          4
NUP50               131        172        154        413        289        319
CTA-268H5.12        0          0          0          0          1          0
KIAA0930            312        364        315        260        166        191
MIR1249             1          2          4          0          1          1
CTA-268H5.14        1          3          1          0          0          0
CTA-268H5.9         0          0          0          0          0          0
UPK3A               0          0          0          2          1          1
FAM118A             53         50         50         113        66         97
SMC1B               3          0          4          6          11         9
RIBC2               0          4          1          12         11         4
RP1-102D24.5        0          0          0          0          1          0
FBLN1               43         47         33         122        96         116
LINC01589           0          0          0          3          1          0
RNU6-1161P          0          0          0          1          0          0
CTA-941F9.10        16         22         15         31         17         29
ATXN10              193        253        246        199        154        170
MIR4762             1          0          0          0          1          1
RP1-37M3.8          0          3          2          5          1          0
WI2-85898F10.2      0          4          3          0          1          0
WI2-85898F10.1      1          0          0          0          0          0
WNT7B               7          11         6          9          9          17
CR536603.1          0          0          0          0          0          0
CITF22-92A6.2       3          2          2          4          5          3
CITF22-92A6.1       0          0          1          1          6          4
LINC00899           7          14         9          7          5          8
PRR34               7          12         15         7          2          3
RP6-109B7.5         4          8          3          0          0          0
PRR34-AS1           6          13         5          15         5          10
MIRLET7BHG          82         137        87         61         56         52
RP6-109B7.2         10         14         11         2          1          6
RP6-109B7.4         17         15         12         6          6          8
MIR3619             2          2          1          5          1          2
MIRLET7A3           0          0          0          0          0          0
MIR4763             0          0          0          0          0          0
MIRLET7B            0          0          0          0          0          0
PPARA               72         101        84         193        147        138
RP4-695O20.1        0          1          1          3          5          4
CDPF1               15         13         11         28         11         19
PKDREJ              6          6          7          8          12         8
TTC38               29         28         20         90         71         85
GTSE1-AS1           2          2          3          2          2          3
GTSE1               2          2          1          145        111        118
TRMU                64         78         75         191        111        140
CELSR1              0          6          4          250        136        195
RP5-996D20.3        0          0          0          0          0          0
RP3-439F8.1         0          0          0          0          9          1
GRAMD4              97         106        75         156        96         135
CERK                209        249        268        298        188        243
CTA-29F11.1         2          6          12         14         13         15
TBC1D22A            39         52         47         78         50         74
U51561.1            1          1          2          1          4          2
FP325331.1          2          4          3          2          1          3
RP1-111J24.1        1          1          0          1          3          0
CITF22-49D8.1       0          1          0          0          2          0
LL22NC03-75H12.2    0          0          0          0          1          0
LINC00898           7          6          1          5          3          1
RP11-191L9.4        8          8          8          32         28         21
RP11-191L9.5        1          0          2          0          2          1
RP13-455A7.1        1          0          0          2          3          0
CTA-280A3.2         4          3          3          7          12         14
CTA-280A3__B.2      0          0          0          0          0          0
LL22NC03-27C5.1     0          0          0          0          2          0
LL22NC03-121E8.4    0          0          0          0          1          0
LL22NC03-121E8.3    0          0          0          0          0          0
MIR3201             0          0          0          0          0          0
RP11-536P6.3        0          0          0          0          0          0
FAM19A5             140        163        151        18         16         14
CTA-299D3.8         17         16         18         0          1          1
MIR4535             0          0          0          0          1          0
AL954742.1          0          0          0          0          0          1
LINC01310           8          7          6          0          9          1
RPL35P8             0          0          0          0          1          0
RP1-34P24.3         0          0          0          0          3          0
C22orf34            2          4          2          36         25         23
CTA-722E9.1         0          0          0          0          3          3
RP4-566L20.1        0          0          0          0          1          5
MIR3667             0          0          0          0          0          0
RP1-29C18.10        0          0          1          13         19         18
RP1-29C18.9         0          0          0          7          1          6
RP1-29C18.8         1          0          1          17         14         8
RP5-983L19.2        0          0          0          0          4          0
RN7SKP252           0          0          0          0          0          0
RPL5P35             0          0          0          0          1          0
BRD1                92         116        103        186        121        160
RP3-522J7.7         3          2          8          5          8          3
RP3-522J7.5         2          3          2          3          3          3
RP3-522J7.6         1          2          1          1          2          0
RP11-494O16.3       0          1          0          0          0          1
RP11-494O16.4       0          0          0          2          0          0
ZBED4               51         68         77         196        153        157
ALG12               21         30         40         53         27         30
CITF22-1A6.3        0          0          0          1          0          1
CRELD2              30         40         43         74         64         57
CITF22-49E9.3       0          0          0          1          4          2
PIM3                47         59         55         159        103        118
MIR6821             0          0          0          0          0          0
IL17REL             0          1          2          0          5          2
TTLL8               0          0          0          0          0          1
MLC1                297        324        307        13         12         12
MOV10L1             10         8          8          0          7          1
RP5-898I4.1         1          0          0          0          1          0
PANX2               59         71         74         9          7          7
TRABD               33         40         37         103        79         87
RP3-402G11.25       2          7          3          4          5          5
RP3-402G11.26       9          17         7          2          0          2
SELO                52         72         33         77         37         65
RP3-402G11.27       7          2          0          5          1          2
RP3-402G11.28       2          2          3          4          2          3
TUBGCP6             248        289        237        279        174        206
HDAC10              4          9          12         9          9          7
MAPK12              60         57         56         116        73         114
MAPK11              64         98         82         25         23         29
PLXNB2              241        301        248        475        285        370
DENND6B             61         101        67         36         20         20
XX-C283C717.1       0          1          0          0          0          0
XX-C00717C00720L.1  0          0          0          0          0          0
PPP6R2              170        249        259        227        150        185
RN7SL500P           0          0          1          0          0          1
SBF1                682        840        696        530        339        422
ADM2                0          2          1          27         21         31
MIOX                0          0          0          1          1          1
LMF2                57         73         69         174        113        164
NCAPH2              86         93         78         219        131        176
SCO2                4          24         10         19         14         22
CTA-384D8.36        8          12         8          7          1          9
TYMP                23         24         30         58         22         33
ODF3B               9          11         9          11         12         11
CTA-384D8.35        0          1          0          0          1          0
CTA-384D8.34        0          0          0          0          0          0
CTA-384D8.31        0          0          0          6          2          0
KLHDC7B             0          0          1          3          8          1
SYCE3               0          0          1          0          0          0
CPT1B               5          3          5          9          8          7
CHKB-CPT1B          2          4          2          6          5          5
CHKB                6          8          4          11         8          4
CHKB-AS1            5          1          3          1          2          1
CTA-384D8.33        0          2          4          1          2          1
MAPK8IP2            410        545        482        13         17         9
ARSA                53         55         65         63         47         53
SHANK3              363        456        375        43         25         35
RNU6-409P           0          0          0          0          0          0
AC000036.4          0          2          1          0          1          0
ACR                 0          0          0          0          2          0
AC002056.5          0          0          0          0          0          0
AC002056.3          0          0          0          0          0          0
RPL23AP82           41         59         54         32         23         34
RABL2B              74         62         54         68         50         47

Word, Character, and Line Count in a File

The wc command is used to obtain word, character, and line count in a file.

wc file.txt

The output (from left to right) for wc can be interpreted as follows.

  • 2 indicates that the file has two lines.
  • 23 indicates that the file has 23 words.
  • 135 indicates that the file has 135 characters.
  • The file in which statistics were generated (ie. file.txt).
  2  23 135 file.txt

The wc results above can be obtained separately. For instance, to just get the number of lines in a file include the -l option.

wc -l file.txt
2 file.txt

Word count can be obtained using the -w option.

wc -w file.txt
23 file.txt

Character count can be obtained uisng the -m option.

wc -m file.txt
135 file.txt

Pattern Searching

Sometimes users may want to search for a keyword in a file. The grep command can be used to do this. The grep command prints every line in a file that contains the search pattern. The arguments in grep are as follows.

  • Search pattern (ie. Linux)
  • File to search (ie. file.txt)
grep Linux file.txt
Linux is a variety of Unix, and sometimes the names are used interchangeably.

To search for lines that do not contain a pattern include the -v option.

grep -v Linux file.txt
Unix is an operating system, just like Windows or MacOS.

What would happen if "linux" with a lower case "l" is used as the search pattern instead of an upper case "L" as shown in the file.txt? Nothing is returned because grep is case sensitive. To ignore case, include the -i option.

grep linux file.txt
grep -i linux file.txt
Linux is a variety of Unix, and sometimes the names are used interchangeably.

Working with Software Installed on Biowulf

The module command is important for anyone who wishe to work with software that are installed on Biowulf. This avail option enables users to browse the software that are available on the cluster.

module avail

Glimpse of module avail results. Users can scroll up/down using the arrow keys to learn what software are available. Hit Q to exit the module avail results and return to the prompt.

----------------------------------------------------- Global Aliases -----------------------------------------------------
   bowtie1        -> bowtie/1.3.1                  deeptrio/1.6.0 -> deepvariant/1.6.0-deeptrio
   bowtie2        -> bowtie/2-2.5.3                deeptrio/1.6.1 -> deepvariant/1.6.1-deeptrio
   deeptrio/1.5.0 -> deepvariant/1.5.0-deeptrio

-------------------------------------------- /data/classes/BTEP/apps/modules ---------------------------------------------
   biostars/1.0

---------------------------------------------- /usr/local/lmod/modulefiles -----------------------------------------------
   3DSlicer/4.8.1                                           hwloc/2.9.3/gcc-8.5.0
   3DSlicer/5.2.2                               (D)         hwloc/2.9.3/gcc-9.2.0

To find if a particular software is available include the software name with the avail option.

module avail fastqc
----------------------------------------------------- Global Aliases -----------------------------------------------------


---------------------------------------------- /usr/local/lmod/modulefiles -----------------------------------------------
   fastqc/0.11.8    fastqc/0.11.9    fastqc/0.12.1 (D)

  Where:
   D:  Default Module

Module defaults are chosen based on Find First Rules due to Name/Version/Version modules found in the module tree.
See https://lmod.readthedocs.io/en/latest/060_locating.html for details.

If the avail list is too long consider trying:

"module --default avail" or "ml -d av" to just list the default modules.
"module overview" or "ml ov" to display the number of modules for each name.

Use "module spider" to find all possible modules and extensions.
Use "module keyword key1 key2 ..." to search for all possible modules matching any of the "keys".

To get a description of a software use module whatis.

module whatis fastqc
fastqc/0.12.1       : fastqc: It provide quality control functions to next gen sequencing data.
fastqc/0.12.1       : Version: 0.12.1

Warning

Upon signing onto Biowulf, users will land in the "log-in" node, which is not meant for analyzing data or transferring files. To perform these task, request time and resources on a compute node using the sinteractive command. To learn more about the sinteractive command, see https://bioinformatics.ccr.cancer.gov/docs/unix-on-biowulf-2024/Lesson4/#requesting-an-interactive-session

To load a software use module load followed by the name of the package.

module load samtools
[+] Loading samtools 1.19  ...

Getting Help

To get help, use the man command which pulls up the manual for a command. Use the up and down arrow keys to scroll through the manual and learn about the different options. Hit q to return to exit man and return to the prompt.

For instance, to pull up the manual for grep, do the following.

man grep

Glimpse of man command output for grep.

GREP(1)                             General Commands Manual                             GREP(1)

NAME
       grep, egrep, fgrep - print lines matching a pattern

SYNOPSIS
       grep [OPTIONS] PATTERN [FILE...]
       grep [OPTIONS] -e PATTERN ... [FILE...]
       grep [OPTIONS] -f FILE ... [FILE...]

DESCRIPTION
       grep searches for PATTERN in each FILE.  A FILE of “-” stands for standard input.  If no
       FILE is given, recursive  searches  examine  the  working  directory,  and  nonrecursive
       searches read standard input.  By default, grep prints the matching lines.

       In  addition,  the variant programs egrep and fgrep are the same as grep -E and grep -F,
       respectively.   These  variants  are  deprecated,  but   are   provided   for   backward
       compatibility.

OPTIONS
   Generic Program Information
       --help Output a usage message and exit.

       -V, --version
              Output the version number of grep and exit.

Continual Learning