Over Representation Analysis

In addition to GSEA, over representation analysis is another method for determining which molecular biology functions, component, etc. are affected as a result of gene expression between biological conditions. Essentially, statistics such as Fisher Exact test are used to determine whether the genes in a differential expression list occur in or overlap with those participating in specific biological functions by chance. To learn about over representation analysis, please refer to https://bioinformatics.ccr.cancer.gov/docs/btep-coding-club/CC2023/FunctionalEnrich_clusterProfiler/.

The filtered differential analysis table will be used as input for over representation analysis (see video below for steps).

In the results table, users can click the link under the "Gene set" column to view the pathway along with information such as enrichment score, p-value, and false discovery rate (FDR).