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Workshop Overview

Description

Metatranscriptomics is the exploration of microbial gene expression, identifying microbes that are alive and performing essential activities in your environment of interest. In this two-part webinar, we will explore how metatranscriptomics can lead to new scientific discoveries, including the benefits and barriers to adoption. We’ll also explore the user-friendly Nephele platform, which makes the bioinformatics analysis easy and accessible for anyone hoping to apply metatranscriptomics in their studies.

Day 1: Foundations of Metatranscriptomics (7/28/2026)

  • Learn the core concepts of metatranscriptomics and discover how microbial gene expression data can be used to identify active microorganisms, understand community function, and address biological research questions.

Day 2: Practical Analysis with Nephele (7/30/2026).

  • Apply metatranscriptomic analysis methods using the Nephele platform, with guided instruction on workflow selection, data inputs, result interpretation, and best practices for reproducible analysis.

Prerequisites

This workshop is intended for a beginner audience. No prior knowledge is required. Attendees will need to register for a Nephele account at https://nephele.niaid.nih.gov. See How to register for Nephele for further instructions.

Questions

This two-day workshop was presented by the Nephele Team, an interdisciplinary group within the Bioinformatics and Computational Biosciences Branch (BCBB) of the NIAID Office of Cyber Infrastructure and Computational Biology (OCICB) in the National Institute of Allergy and Infectious Diseases (NIAID). Please direct Nephele related questions to the Nephele Support Team. For all other questions, feel free to contact the Bioinformatics Training and Education Program (BTEP) at ncibtep@nih.gov.

Recordings

All events will be recorded and made available on the BTEP Video Archive 24 to 48 hours following the event.