Practical Analysis with Nephele

Learning Objectives

Apply metatranscriptomic analysis methods using the Nephele platform, with guided instruction on workflow selection, data inputs, result interpretation, and best practices for reproducible analysis.

Getting a Nephele Account

A Nephele account is required for hands-on participation. Need a Nephele account? See How to register for Nephele for further instructions.

Presentation Slides

Step-by-step instructions to reference during the demo are available in the presentation slides.

Data

Demo Data

Demo data for this workshop are available in a zipped directory Demo_Inputs. Simply, click the link to download the Inputs directory. This zipped file contains a mapping file and a directory of subsampled fastq files.

Example Output

Example output files from the demo are available in quality_check_metatranscriptomics.zip and wgsa2_metatranscriptomics_example_output_3_samples.zip. These zipped files contain example outputs from QC and WGSA2 pipelines, respectively, using the demo dataset. Simply, click the links to download the zipped files.

For the next ~90 days, you can access the results on the Nephele website using the following links:

• QC Pipeline
• WGSA2+ Pipeline