From One Cell to Multiple Molecular Views: A Practical Introduction to Single-Cell Multi-omics
Speaker: Stefan Cordes, PhD, MD (NHLBI)
This session introduced major concepts and practical workflows for single-cell multi-omics, with emphasis on paired same-cell measurements such as CITE-seq and single-cell RNA+ATAC multiome, as well as integration of unpaired and spatial modalities. Dr. Cordes discussed how transcriptomic, protein, chromatin-accessibility, genotype/repertoire, perturbation, and spatial readouts can be combined to improve cell-state annotation and biological interpretation, with practical examples comparing Seurat/Signac and scverse-oriented workflows.
Presentation Slides
Workflow Resources
- A Juptyer Notebook of scverse-orinted workflows: multiomics_scverse.ipynb
- An RMarkdown Notebook of Seurat/Signac-oriented workflows: multiomics_seurat_signac.Rmd