QC to Clustering
Speaker: Alex Emmons, PhD (BTEP)
Date: May 17, 2026
This seminar introduced the major steps in a standard single-cell RNA-seq pre-processing analysis workflow, from quality control and filtering through normalization, dimensionality reduction, and clustering. This presentation focused on the reasoning behind each step, common choices researchers face, and how these early analytical decisions shape downstream interpretation of cell populations and biological results.
Open the Full Report
Get the Data
The R code and data used to generate this report can be downloaded at https://bioinformatics.ccr.cancer.gov/docs/singlecell-spatial-2026/IntroSeurat2026/scRNASeq2026_Example.zip.
Get the slides
The slides from this presentation are available here.