Basic Single Cell RNA-Seq Analysis & Visualization in Partek Flow

About this Class

This one and a half hour online training will provide a demonstration of how to identify cell types based on statistics, visualization, and canonical markers. One Peripheral blood mononuclear cells (PBMCs) sample will be used to illustrate a basic Single Cell RNA-Seq analysis pipeline. Partek Flow is a web-based application for the analysis of next generation sequencing (NGS) including RNA, small RNA, and DNA sequencing. With an easy-to-use graphical interface and the ability to build custom analysis pipelines, Partek Flow enables users to carry out routine NGS data analysis using dozens of popular algorithms without writing codes or running command lines tools.  Attendees will need to have taken the Partek Flow Basic Components class before registering or be comfortable with Partek Flow.

By the end of this training, attendees  will be able to:

• Describe how to access Partek Flow from the NIH Library
• Discuss the Quality Control (QC) and Quality Assurance (QA) tools
• Normalize data
• Descriptions options for cell type classification
• Perform differential analysis

Requirements

Access to the NIH Library Partek Flow requires two accounts:

1. Attendees will need to have taken the Partek Flow Basic Components class before registering or be comfortable with Partek Flow.
2. Access any of the NIH HPC resources require an NIH HPC account. NIH HPC accounts are restricted to NIH employees and contractors as determined by inclusion in the NIH Enterprise Directory. You can request an account from NIH HPC Account page. 
3. You also need to register for a Partek Flow account through the NIH Library.

Note on Technology

Registrants will receive an email with information and instructions to verify Partek Flow before the class. If you register the day before the class, you may not have time to secure access to Partek Flow. If you do not have the software installed, this training will be demo only.