About this Class

Please bring a laptop. If you need to borrow a laptop please contact ncibtep@nih.gov No software installation is needed as this program will run on a  web browser. iCn3D provides a powerful, web-based platform to visualize and analyze proteins complexes in 1D (sequence) and 3D(structure), from single domains through large viruses. This hands-on training is geared towards non-expert users with no to little knowledge in protein structure, or experienced users that do not know iCn3D. We will learn how to use it for 3D visualization, interactive protein structure analysis, visualization of SNPs on 3D structure, and sharing annotations with collaborators. Agenda: 2:30-3:45,  Hitting the road running on protein structure analysis

• Analysis of the T-cell receptor complex structure that was recently solved (TCRɑβ/CD3εσ/CD3εδ/CD3ζ, PDB ID 6JXR, https://www.ncbi.nlm.nih.gov/Structure/pdb/6JXR)
• Look at P53 and P53-DNA interactions (PDB ID 1TUP, https://www.ncbi.nlm.nih.gov/Structure/pdb/1TUP), map SNPs from dbSNP and ClinVar and look at potential SNPs disruptions of key interactions.

4:00-4:30,  Play with the protein of your choice Reference/Documentation on iCn3D paper: http://dx.doi.org/10.1093/bioinformatics/btz502 documentation: https://www.ncbi.nlm.nih.gov/Structure/icn3d/docs/icn3d_help.html If you are unable to attend in person, WebEx will be provided: https://cbiit.webex.com/cbiit/j.php?MTID=m375cb4351aefde1b93ae4dc3b9dbbfda