ncibtep@nih.gov

Bioinformatics Training and Education Program

BTEP: Introduction to RNA-Seq technology, Overview and Analyses

BTEP: Introduction to RNA-Seq technology, Overview and Analyses

 When: Nov. 5th, 2018 9:30 am - 11:30 am

To Know

Where:
Bldg 10: FAES Classroom 3 (B1C207)
Organizer:
BTEP
Presented By:
Peter FitzGerald (GAU)
Class Files:
Files
This class has ended.
Video Archive *Class recordings may take ~48 hours to become available.

About this Class

This introductory lecture will provide an overview of RNA-Seq technology, its various application and shortcomings, as well as detailing the steps involved in analyzing the resulting data. The specific topics covered include:
  1. What is RNASeq ?
  2. What can it be used for ?
  3. Sequencing platforms
  4. Quality Control steps
  5. Experimental Design
  6. Data Analysis Workflows
  7. Identifying differentially expressed genes
  8. Discussion of relevant file formats and data conversion tools
  9. Approaches to detect splice variants and fusion genes
  10. NCI specific tools and software

Files

  • RNASeq_Final.pdf: |