Custom Spatial Biology Analysis Pipelines for NCI CCR Researchers from the High-Throughput Imaging Facility (HiTIF)
To Know
About this Class
Gil Kanfer, PhD, of the NCI CCR High-Throughput Imaging Facility (HiTIF), in the Laboratory of Receptor Biology and Gene Expression (LRBGE), will present the spatial biology analysis stack HiTIF is building to support Center for Cancer Research (CCR) researchers, with a focus on high-resolution spatial transcriptomics and multiplex protein imaging platforms existing in CCR Cores (e.g., Visium HD, Xenium-5k, CODEX) and how they can be turned into robust, reusable analysis workflows.
Using recent liver cancer and melanoma projects run by CCR investigators with the NCI CCR Single Cell Analysis Facility (SCAF) and Spatial Imaging Technology Resource (SpITR) core facilities in collaboration with HiTIF as examples, he will show how in-house algorithms—such as a zonation prediction model for mapping periportal vs pericentral regions and custom methods for collagen-based proximity and niche analysis—are combined with open-source tools to align images, integrate RNA and protein data, quantify cell-type composition and spatial organization, and systematically screen ligand–receptor interactions across conditions and time points. The talk will emphasize generalizable, technology-driven pipelines that take core-generated images all the way to quantitative, biologically interpretable spatial-omics readouts for CCR labs.
Attendance at this event is limited to NCI CCR personnel.