Mapping the human gastrointestinal tract through space and time

About this Class

Sarah Teichmann Head of the Cellular Genetics Programme - Wellcome Sanger Institute - Cambridge UK Lab homepage: https://www.sanger.ac.uk/group/teichmann-group/ Venue: zoom conference https://nih.zoomgov.com/j/1619988709?pwd=dEIwM0ExOXFtSjNqd0xmcGJCVEk5QT09 Meeting ID: 161 998 8709 Passcode: 20892 Summary The cellular landscape of the human gastrointestinal tract is dynamic throughout life, changing in response to changing functional requirements and environmental exposures. While the human intestines has been explored in depth, we present a comprehensive single-cell analysis across gut regions, and throughout development, adulthood for the first time. Using single-cell RNAseq and VDJ analysis, we survey all cell lineages in the healthy developing, paediatric and adult human gut, including 347,980 cells from up to 10 distinct anatomical sites. We identify BEST4+ enterocytes throughout the gut and implicate Tuft cells in IgG sensing. We define novel cell populations in the developing enteric nervous system and show patterned expression of irritable bowel syndrome and Hirschsprung’s disease. In addition, we identify key cell players and communication networks initiating lymphoid structure formation in early human development. We show that lymphoid organogenesis programs are adopted in inflammatory bowel disease to recruit and retain immune cells at the site of inflammation. Brief Bio Sarah Teichmann is interested in global principles of regulation of gene expression and protein complexes, with a focus on immunity. Sarah did her PhD at the MRC Laboratory of Molecular Biology, Cambridge, UK and was a Beit Memorial Fellow at University College London. She started her group at the MRC Laboratory of Molecular Biology in 2001, discovering stereotypical pathways of assembly and evolution of protein complexes during this time. In 2013, she moved to the Wellcome Genome Campus in Hinxton/Cambridge, jointly with the EMBL-European Bioinformatics Institute and the Wellcome Sanger Institute (WSI). In February 2016 she became Head of the Cellular Genetics Programme at the WSI and co-founded the Human Cell Atlas international initiative which she continues to lead. Sarah was elected a member of EMBO in 2012, a fellow of the Academy of Medical Sciences in 2015 and a fellow of the Royal Society in 2020. For more information, contact: Gregoire.altan-bonnet@nih.gov