Molecular atlassing with MERSCOPE reveals the spatial organization of mouse and human tissues

About this Class

https://nih-irp-singlecell.github.io/SC-UsersGroup/ Presenter: George Emanuel PhD, Co-founder Vizgen, Director of Technology and Partnerships. Abstract: Biological systems are comprised of numerous cell types, intricately organized to form functional tissues and organs. Cell atlas initiatives with single-cell RNA sequencing have begun to characterize cell types based on their RNA expression profiles. However, the tissue organization is lost when cells are dissociated for single-cell sequencing, making it difficult to study how the cellular heterogeneity is contributing to the function of the tissue. This talk introduces a technology which enables in situ profiling of the spatial organization of intact tissue with genomic-scale throughput. It permits spatial profiling of hundreds of thousands of cells with high accuracy and reproducibility through combinatorial labeling, sequential imaging, and error-robust barcoding. The talk will highlight various, including mapping GPCR expression across the mouse brain, identifying rare blood cells by measuring millions of PBMCs, and characterizing the immune landscape and microenvironment of a human colon cancer tumor. Biography: Dr. Emanuel is trained as a biophysicist at Harvard University in the lab of Dr. Xiaowei Zhuang. For the past decade he has worked on the development of highly multiplexed RNA fluorescence in situ hybridization-based technologies. George is a scientific cofounder at Vizgen, where he is currently Director of Technology and Partnerships.