Oxford Nanopore Technologies Long Read Sequencing Live Workshop: Human Variation Data Analysis
To Know
About this Class
Are you looking to expand the reach of your sequencing to enable what long read technologies can provide? Please join Oxford Nanopore Technologies (ONT) bioinformatics specialists on a deep dive into getting the most from your long read sequencing data.
This workshop will cover the details of analyzing human whole genome sequencing data using our EPI2ME pipeline wf-human-variation. This workflow provides users with tools to perform alignment and variant calling for single nucleotide, structural, and copy number variants as well as clinically relevant short tandem repeats, and cytosine methylation. Participants will learn about the EPI2ME software, pipeline details, and work with an ONT bioinformatics expert in a hands-on data analysis training exercise.
This in-person, hands-on workshop gives learners the opportunity to learn the Nanopore Human Variation Data Analysis Software Workflow. Taught by Nanopore personnel, with assistance from the CCR Genomics Core and BTEP, this session will run in the afternoon. Space is limited. If your plans change and you cannot attend, please cancel your registration. There will be no hybrid option for this class, it is in-person only.
1:30-1:45 – Check in & distribute materials
1:45-2:15 – Data analysis intro from ONT (MinKNOW/EPI2ME/other advanced tools)
2:15-2:45 – Introduction to the Human Variation (WGS) pipeline
2:45-3:00 - Break
3:00-4:00 – Hands on data analysis: human variation EPI2ME App, nextflow command line, and Biowulf HPC demo
4:00-4:30 – Review result
4:30-5:00 – Closing/Q&A